BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0413.0003.p (216 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_655392.1| hypothetical protein predicted by GeneMark [Bac... 159 5e-38 ref|NP_242486.1| BH1620~unknown conserved protein in bacilli [Ba... 159 6e-38 ref|NP_831269.1| genetic competence negative regulator mecA [Bac... 157 2e-37 ref|NP_390178.1| similar to negative regulation of competence Me... 152 6e-36 ref|NP_692732.1| negative regulation of genetic competence [Ocea... 127 2e-28 >ref|NP_655392.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_843961.1| negative regulator of competence MecA, putative [Bacillus anthracis str. Ames] ref|NP_977937.1| negative regulator of competence MecA, putative [Bacillus cereus ATCC 10987] sp|Q81SY4|MEA2_BACAA Adapter protein mecA 2 gb|AAP25447.1| negative regulator of competence MecA, putative [Bacillus anthracis str. Ames] gb|AAS40545.1| negative regulator of competence MecA, putative [Bacillus cereus ATCC 10987] Length = 202 Score = 159 bits (401), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 88/207 (42%), Positives = 129/207 (62%), Gaps = 8/207 (3%) Query: 10 MRIERLSQDKIRIFLTFDDLSERGIQKEDMWQEVPKVHDLFTEMMDQAYSELGFDATGPL 69 MR+ERL+ +KI+IFLTFDDLSERG+ KED+W+ PKV LF +MM +A ELGF+A GP+ Sbjct: 1 MRLERLNYNKIKIFLTFDDLSERGLTKEDLWRNAPKVQQLFRDMMQEANKELGFEADGPI 60 Query: 70 XXXAVEVFALPAQGMVVIVTRGKYDHHQYGASGXXXXXXXXXXXXVTLEQSDSIVYAFRD 129 AVEVF+L AQGMVVIVT+ HQ A VTL++S+ I+Y F Sbjct: 61 ---AVEVFSLQAQGMVVIVTK----EHQ-EADTDDEFRDEFIEMQVTLDESEHILYEFAT 112 Query: 130 FEALVEAAHVLIGNITSQGKLYSYNDIWYLYFDPKEFEEAALSGLVGVLSEFGDSSPVTE 189 + ++ ++ L + GKLY+++ +YL+ + +E + + + +L+E+G+ S T Sbjct: 113 LDDVINLSNRLYNLDVTGGKLYTWDGRFYLWMEEEEQIQLLKADFIAILAEYGNPSTATI 172 Query: 190 AMLAEYGKAVMPENAIELICTHFKRQQ 216 L EYGK +M AIE I +F ++Q Sbjct: 173 YRLMEYGKELMASQAIEQIHNYFVKKQ 199 >ref|NP_242486.1| BH1620~unknown conserved protein in bacilli [Bacillus halodurans] sp|Q9KCF1|MEA1_BACHD Adapter protein mecA 1 pir||D83852 hypothetical protein BH1620 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05339.1| BH1620~unknown conserved protein in bacilli [Bacillus halodurans] Length = 201 Score = 159 bits (401), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 88/207 (42%), Positives = 127/207 (61%), Gaps = 8/207 (3%) Query: 10 MRIERLSQDKIRIFLTFDDLSERGIQKEDMWQEVPKVHDLFTEMMDQAYSELGFDATGPL 69 MR+ERL+ +KI++FLTFDDL ERGI K+++WQ++PKVHDLF +MM +A ELGF A GP+ Sbjct: 1 MRLERLAVNKIKVFLTFDDLKERGITKDELWQDIPKVHDLFRDMMLEADDELGFKADGPI 60 Query: 70 XXXAVEVFALPAQGMVVIVTRGKYDHHQYGASGXXXXXXXXXXXXVTLEQSDSIVYAFRD 129 AVEVFALPAQGMV+IVT+G HQ VTL++SD + Y F D Sbjct: 61 ---AVEVFALPAQGMVIIVTKG----HQESDFDEDGLEDGYIEMQVTLDESDEVFYEFAD 113 Query: 130 FEALVEAAHVLIGNITSQGKLYSYNDIWYLYFDPKEFEEAALSGLVGVLSEFGDSSPVTE 189 E ++ L+ + G LYSY +Y+ F + + L+ +L+E+G S ++ Sbjct: 114 IEDVISLVPRLVTLGMTGGTLYSYQGRYYISFTDLDLDGIDEESLIALLAEYGYPSTIST 173 Query: 190 AMLAEYGKAVMPENA-IELICTHFKRQ 215 +AEYG ++ +A I+L + FK + Sbjct: 174 YRVAEYGSLIIANDACIKLYQSFFKAR 200 >ref|NP_831269.1| genetic competence negative regulator mecA [Bacillus cereus ATCC 14579] sp|Q81FS9|MEA2_BACCR Adapter protein mecA 2 gb|AAP08470.1| genetic competence negative regulator mecA [Bacillus cereus ATCC 14579] Length = 202 Score = 157 bits (396), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 85/207 (41%), Positives = 129/207 (62%), Gaps = 8/207 (3%) Query: 10 MRIERLSQDKIRIFLTFDDLSERGIQKEDMWQEVPKVHDLFTEMMDQAYSELGFDATGPL 69 MR+ERL+ +KI+IFLTFDDLSERG+ KED+W+ PKV LF +MM +A ELGF+A GP+ Sbjct: 1 MRLERLNYNKIKIFLTFDDLSERGLTKEDLWRNAPKVQQLFRDMMQEANKELGFEADGPI 60 Query: 70 XXXAVEVFALPAQGMVVIVTRGKYDHHQYGASGXXXXXXXXXXXXVTLEQSDSIVYAFRD 129 AVEVF+L AQGMVVIVT+ + VTL++S+ I+Y F Sbjct: 61 ---AVEVFSLQAQGMVVIVTKENQE-----MDTDDEFRDEFIEMQVTLDESEHILYEFAT 112 Query: 130 FEALVEAAHVLIGNITSQGKLYSYNDIWYLYFDPKEFEEAALSGLVGVLSEFGDSSPVTE 189 + ++ ++ L + GKLY++++ +YL+ + +E + + + +L+E+G+ S T Sbjct: 113 LDDVINLSNRLYNLGVTGGKLYTWDERFYLWVEEEEQIQLLKADFIAILAEYGNPSTATI 172 Query: 190 AMLAEYGKAVMPENAIELICTHFKRQQ 216 + EYGK +M NAIE I +F ++Q Sbjct: 173 YRIMEYGKELMDVNAIEQIHNYFVKKQ 199 >ref|NP_390178.1| similar to negative regulation of competence MecA homolog [Bacillus subtilis] sp|P50734|MEA2_BACSU Adapter protein mecA 2 pir||C69933 negative regulation of competence MecA hom homolog ypbH - Bacillus subtilis gb|AAC83952.1| putative [Bacillus subtilis] emb|CAB14213.1| ypbH [Bacillus subtilis subsp. subtilis str. 168] Length = 194 Score = 152 bits (383), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 84/203 (41%), Positives = 126/203 (62%), Gaps = 10/203 (4%) Query: 10 MRIERLSQDKIRIFLTFDDLSERGIQKEDMWQEVPKVHDLFTEMMDQAYSELGFDATGPL 69 MR+ERL+ +KI+IFLT DDL++RG+ KED+W++ KVH LF +MM++A +ELGF+A GP+ Sbjct: 1 MRLERLNYNKIKIFLTLDDLTDRGLTKEDLWKDSFKVHQLFKDMMNEANTELGFEANGPI 60 Query: 70 XXXAVEVFALPAQGMVVIVTRGKYDHHQYGASGXXXXXXXXXXXXVTLEQSDSIVYAFRD 129 AVEV++L AQGMVVIVT+ +Q S V L++S I+Y F Sbjct: 61 ---AVEVYSLQAQGMVVIVTK-----NQDADSEDDEYDDDYIEMQVKLDESADIIYQFHS 112 Query: 130 FEALVEAAHVLIGNITSQGKLYSYNDIWYLYFDPKEFEEAALSGLVGVLSEFGDSSPVTE 189 FE +++ + L + G +Y Y+ ++L + + G+V VL+E+G+ + +T Sbjct: 113 FEDIIQLSESLQRIGITGGTVYHYDGQYFLSLE--DLGSHTAEGVVAVLAEYGNPTTLTI 170 Query: 190 AMLAEYGKAVMPENAIELICTHF 212 L EYGK +M NA+E I THF Sbjct: 171 YRLQEYGKLIMDGNAVETIQTHF 193 >ref|NP_692732.1| negative regulation of genetic competence [Oceanobacillus iheyensis HTE831] sp|Q8EQ97|MECA_OCEIH Adapter protein mecA dbj|BAC13767.1| negative regulation of genetic competence [Oceanobacillus iheyensis HTE831] Length = 192 Score = 127 bits (318), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 73/203 (35%), Positives = 109/203 (53%), Gaps = 12/203 (5%) Query: 10 MRIERLSQDKIRIFLTFDDLSERGIQKEDMWQEVPKVHDLFTEMMDQAYSELGFDATGPL 69 MRIER+S D+ +IFLTFDDL ERG +ED+W + V LF++MM +A SELG + G L Sbjct: 1 MRIERVSTDQFKIFLTFDDLIERGFTREDLWHDASNVRSLFSDMMYEASSELGIELEGML 60 Query: 70 XXXAVEVFALPAQGMVVIVTRGKYDHHQYGASGXXXXXXXXXXXXVTLEQSDSIVYAFRD 129 V+V + AQGM + VT+ D+ + VTL++S ++++F D Sbjct: 61 ---LVQVHLMQAQGMHIFVTQQYEDNSE---------DEDYIEMKVTLDESKELIFSFMD 108 Query: 130 FEALVEAAHVLIGNITSQGKLYSYNDIWYLYFDPKEFEEAALSGLVGVLSEFGDSSPVTE 189 FE ++ L +LY D +Y+ D E ++GV+SE+ + S VT Sbjct: 109 FEDIISVTSYLDPMGLENIRLYYMEDRYYMILDHIELSRVDKEDIIGVMSEYANPSIVTS 168 Query: 190 AMLAEYGKAVMPENAIELICTHF 212 + EYGK +M EN ++ I F Sbjct: 169 HRIEEYGKLIMEENTVQQIKRFF 191 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 280,163,117 Number of extensions: 9979430 Number of successful extensions: 23131 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 23116 Number of HSP's successfully gapped: 25 Length of query: 216 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 95 Effective length of database: 428,710,599 Effective search space: 40727506905 Effective search space used: 40727506905 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)