BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0412.0019.p (203 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_623072.1| conserved hypothetical protein [Thermoanaerobac... 103 2e-21 ref|NP_563281.1| conserved hypothetical protein [Clostridium per... 92 6e-18 ref|NP_781960.1| transporter [Clostridium tetani E88] >gi|282034... 89 6e-17 ref|NP_347071.1| Hypothetical protein, YitT family [Clostridium ... 88 1e-16 ref|NP_242544.1| BH1678~unknown conserved protein [Bacillus halo... 86 4e-16 >ref|NP_623072.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] gb|AAM24676.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] Length = 294 Score = 103 bits (258), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 63/186 (33%), Positives = 101/186 (54%), Gaps = 3/186 (1%) Query: 3 LNNIMISFIGCFIMGLGVNLGYVPVHLFSTGFPGIGVLALYVLDWNTGIVVFALNVPLFL 62 + NI +G FI +G+N+ V L S G GI ++ Y + G +F LN+PL + Sbjct: 18 IKNIFFILVGTFISSIGINMFLVHARLLSGGVSGIALIIQYFTKFPAGYTIFLLNIPLLI 77 Query: 63 LAWKSIGRSFVIQTIIVAGVLSVFLDLLYPIRN--WVHPPLWLGIVVGGAFMGLGAGIVF 120 L++K + F I TII LS FL L Y +RN ++ PL L + GG GLG GIVF Sbjct: 78 LSYKKVNLRFTIFTIIGTAALSFFLVLTYSVRNVLLINDPLLLSL-YGGVLNGLGMGIVF 136 Query: 121 RQGLTSGGVGLLARLIQLHYPKLKMGTIHIGFDFFVLILGAVVMDITTAFYTFLASIIMG 180 ++GG+ +++ +++ Y ++G I +F ++ LGA+ +T+A YT ++ I Sbjct: 137 SNHGSTGGLDIISVIVKKKYDNFEIGRISFTVNFIIVALGAIFFGLTSALYTLVSMYITS 196 Query: 181 RTMDFT 186 +D T Sbjct: 197 YMVDKT 202 >ref|NP_563281.1| conserved hypothetical protein [Clostridium perfringens] dbj|BAB82071.1| conserved hypothetical protein [Clostridium perfringens str. 13] Length = 285 Score = 92.4 bits (228), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 55/166 (33%), Positives = 92/166 (55%), Gaps = 1/166 (0%) Query: 11 IGCFIMGLGVNLGYVPVHLFSTGFPGIGVLALYVLDWNTGIVVFALNVPLFLLAWKSIGR 70 IGCFI LGVNL L S G GI ++ Y++ N+GIVV +N+PLF+L++ + + Sbjct: 19 IGCFIASLGVNLFLSNAKLLSGGATGIALIFQYLMGVNSGIVVLLINIPLFILSYFKLSK 78 Query: 71 SFVIQTIIVAGVLSVFLDLLYPIRNWVH-PPLWLGIVVGGAFMGLGAGIVFRQGLTSGGV 129 F + I LS+ L + P+ + + L V GGA GLG G+VF +G ++GG Sbjct: 79 KFTFNSAIGMLALSLSLMITAPVSHLITLDDKLLYCVFGGAICGLGYGLVFSKGGSTGGT 138 Query: 130 GLLARLIQLHYPKLKMGTIHIGFDFFVLILGAVVMDITTAFYTFLA 175 ++ +I+ Y +G++ + ++ +GA+ + TA YT ++ Sbjct: 139 DIVTMIIRKKYSNFNIGSLSFVLNMCIVAVGAIFFGLETALYTLIS 184 >ref|NP_781960.1| transporter [Clostridium tetani E88] gb|AAO35897.1| transporter [Clostridium tetani E88] Length = 289 Score = 89.0 bits (219), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 56/185 (30%), Positives = 98/185 (52%), Gaps = 1/185 (0%) Query: 1 LKLNNIMISFIGCFIMGLGVNLGYVPVHLFSTGFPGIGVLALYVLDWNTGIVVFALNVPL 60 L L NI+ +G I +G+N V L G G+ ++ Y ++ TG VF +N+PL Sbjct: 10 LSLKNIIFIILGSLISAIGINTFIVNASLLGGGVSGLALILEYTMNIPTGYSVFLINLPL 69 Query: 61 FLLAWKSIGRSFVIQTIIVAGVLSVFLDLLYPI-RNWVHPPLWLGIVVGGAFMGLGAGIV 119 L++K + F I TII + LS+ L + P+ R V + L + GG G+G G+ Sbjct: 70 LYLSYKKMDTRFTINTIIGSVSLSIMLIITRPLQRLVVLDDILLLCIYGGILNGVGVGLS 129 Query: 120 FRQGLTSGGVGLLARLIQLHYPKLKMGTIHIGFDFFVLILGAVVMDITTAFYTFLASIIM 179 F ++GG+ +++ +I+ ++ +G I GF+F +++ GA + DI A YT ++ + Sbjct: 130 FSNQGSTGGLDIISSVIKKNHENFDIGKISFGFNFLIVLAGAFIFDIKIALYTLVSMYLT 189 Query: 180 GRTMD 184 +D Sbjct: 190 ATMID 194 >ref|NP_347071.1| Hypothetical protein, YitT family [Clostridium acetobutylicum] pir||H96952 hypothetical protein CAC0431 [imported] - Clostridium acetobutylicum gb|AAK78411.1| Hypothetical protein, YitT family [Clostridium acetobutylicum] Length = 291 Score = 87.8 bits (216), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 60/182 (32%), Positives = 92/182 (50%), Gaps = 3/182 (1%) Query: 5 NIMISFIGCFIMGLGVNLGYVPVHLFSTGFPGIGVLALYVLDWNTGIVVFALNVPLFLLA 64 NI + IGCF +G+NL + HL S G GI ++ Y+ GI +N+PLFLL+ Sbjct: 16 NIFYTVIGCFTSSIGINLFLIHAHLLSGGVSGISLILQYLFKIPAGISYLIMNLPLFLLS 75 Query: 65 WKSIGRSFVIQTIIVAGVLSVFLDLLYPIRNW--VHPPLWLGIVVGGAFMGLGAGIVFRQ 122 +K IG+ F TI+ SV +L P +N + P+ L V GG GLG GIV Sbjct: 76 YKVIGKKFTAMTILGTLTFSVAFNLTAPFKNLLTIKDPILL-CVYGGVLNGLGMGIVLSN 134 Query: 123 GLTSGGVGLLARLIQLHYPKLKMGTIHIGFDFFVLILGAVVMDITTAFYTFLASIIMGRT 182 + GG+ ++A I+ + + T + + ++ LGA+ + +A YT + I Sbjct: 135 YGSLGGLDIVAAAIKKKHENFQFSTTSLIINILIITLGAIFFGLPSALYTLFSIYISYSV 194 Query: 183 MD 184 MD Sbjct: 195 MD 196 >ref|NP_242544.1| BH1678~unknown conserved protein [Bacillus halodurans] pir||F83859 hypothetical protein BH1678 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05397.1| BH1678~unknown conserved protein [Bacillus halodurans] Length = 290 Score = 86.3 bits (212), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 62/196 (31%), Positives = 96/196 (48%), Gaps = 10/196 (5%) Query: 1 LKLNNIMISFIGCFIMGLGVNLGYVPVHLFSTGFPGIGVLALYVLDWNTGIVVFALNVPL 60 LK NI+ +G I G+ + +L GF GI ++ ++ N + LN+P+ Sbjct: 5 LKWKNIVFILLGSAIFSFGLVYFNMENNLAEGGFTGITLILYFMFQINPAVTNLVLNIPI 64 Query: 61 FLLAWKSIGRSFVIQTIIVAGVLSVFLDLLYPIRNW------VHPPLWLGIVVGGAFMGL 114 L+ WK +GR +I TII +SVFL++ + W +H + L + G F+G Sbjct: 65 LLIGWKILGRVTLIYTIIGTVSVSVFLEMF---QRWKFMDIPLHDDMTLAALFAGVFVGT 121 Query: 115 GAGIVFRQGLTSGGVGLLARLIQLHYPKLKMGTIHIGFDFFVLILGAVVMDITTAFYTFL 174 G GIVFR G T+GGV ++A+L Y MG FD V+ + ++ A YT L Sbjct: 122 GLGIVFRFGGTTGGVDIIAKL-GFRYLGWSMGKTMFMFDAVVIASSLIYLNYREAMYTLL 180 Query: 175 ASIIMGRTMDFTKSVP 190 A I + +DF + Sbjct: 181 AVFIAAKVIDFIQQTA 196 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.332 0.149 0.471 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 309,001,211 Number of extensions: 13437543 Number of successful extensions: 48476 Number of sequences better than 1.0e-05: 162 Number of HSP's gapped: 48276 Number of HSP's successfully gapped: 162 Length of query: 203 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 83 Effective length of database: 431,425,698 Effective search space: 35808332934 Effective search space used: 35808332934 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 123 (52.0 bits)