BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0412.0010.p (421 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060242.1| COG4753: Response regulator containing CheY-l... 139 1e-31 ref|ZP_00014494.1| COG4753: Response regulator containing CheY-l... 125 2e-27 ref|NP_388577.1| similar to two-component response regulator [Ye... 123 1e-26 ref|NP_242975.1| BH2109~unknown conserved protein [Bacillus halo... 120 5e-26 ref|NP_756406.1| Hypothetical protein [Escherichia coli CFT073] ... 106 1e-21 >ref|ZP_00060242.1| COG4753: Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain [Clostridium thermocellum ATCC 27405] Length = 362 Score = 139 bits (351), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 102/351 (29%), Positives = 179/351 (50%), Gaps = 21/351 (5%) Query: 74 RDIVRTFGSWEEHGMEIAGEAEDGREALRLTDELSPQIVITDMRMPGADGVMLMNNLHAR 133 R +++ + WE G +AGEA DGREALR D+ +VI+D++MPG DG+ L++ L Sbjct: 14 RYMLKRYKGWESFGFVLAGEASDGREALRKLDKEPFDVVISDIKMPGMDGIELLSELRNN 73 Query: 134 YPEIKVIVVSGYDDFKYAQNAVRYGAVDYLLKPVDPKELNAVLDKCRRELEEAERGQKAY 193 +I V+ +S + DF YA+ +R G DYL KP + L LD+ + L+E ++ QKA Sbjct: 74 GNDICVLFLSTHSDFSYAKQGIRLGVFDYLTKPFSDETLGEALDRVKVYLDEKKK-QKAL 132 Query: 194 ALDIDVSLCLSSYKQLMRVHFNELNAEGVNSV-----LEGIR---RELELGGAKGPGALR 245 + + SS +V++++ + + + SV LE I R E G + Sbjct: 133 VNIYNKNALESS-----QVYYSKNDEKKLVSVILSGSLEAINLGDRLFEKMAQFTEGDAK 187 Query: 246 QAANEMLLLLKELMRGNRLEDELLK------AEISHEALASAANVEAYLKALYGAALELL 299 + A M +L E+ G +K + S E + E ++ + G + L+ Sbjct: 188 KLAILMENILLEVDEGIYKAIPWIKNLKNRPSNKSFEGMDEKELKERFIDHISG-WVRLV 246 Query: 300 IGQRKFKNKLKLDEVRLYIDGHFAEAVTLDGLARAFFVSKEYLSKVFKQEYGCNVTDYIL 359 + ++ + ++ Y+ H E + ++ +A +VS++Y+ K+FKQ+ G N+++YI Sbjct: 247 VKFELHQSDSLMRKICEYVINHVEEDIKIENIANELYVSRDYIGKLFKQKAGYNLSEYIT 306 Query: 360 GLRMEKAKEWLADENIPIKTVAELAGYEDVGYFYRVFKKHYGVAPGEMRKQ 410 ++ME AK ++ ++E+ GY+ YF +VFK + G P E RK Sbjct: 307 KVKMEHAKYLISKGEYKNYEISEILGYKKADYFSQVFKSYVGCTPSEYRKN 357 >ref|ZP_00014494.1| COG4753: Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain [Rhodospirillum rubrum] Length = 356 Score = 125 bits (315), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 98/356 (27%), Positives = 173/356 (48%), Gaps = 22/356 (6%) Query: 61 MIKVLLVDDESWNRDIVRTFGSWEEHGMEIAGEAEDGREALRLTDELSPQIVITDMRMPG 120 M +++V+DE R +RT + G+ I GEA G EA+ L D +++ D+ +P Sbjct: 1 MYGIVIVEDEELERQALRTILTENIDGIRILGEARTGTEAVALIDSCEIDLMLVDINIPN 60 Query: 121 ADGVMLMNNLHARYPEIKVIVVSGYDDFKYAQNAVRYGAVDYLLKPVDPKELNAVLDKCR 180 +G+ ++ +L R+ + KVI+ + YD F+ + A++ +YLLKP+ + L + C Sbjct: 61 LNGLEVIRHLRDRHADTKVIITTAYDYFETTRTAIQLKVDEYLLKPIRTQALVTTIQSCI 120 Query: 181 RELEEAERGQKAYALDIDVSLCLSSYKQ---LMRVHFNELNAEGVNSVLEGIRRELELGG 237 +L + ++ D+ + Y++ L+R H E + + + R+L L Sbjct: 121 GQLGAGRQCRELVGRIADL-VEQDGYQEGVTLVRRHI-----EWICTQRGHVPRDLILDF 174 Query: 238 AKGPGALRQAANEMLLLLKELMRGN--RLEDELLKAEISHEALASAANVEAYLKALYGAA 295 A GAL A + L L + + +L L A+ ++AL + V + L+ Sbjct: 175 A---GALVALARDKGLRLPDALTQQIGQLRTMPLDAQTGYQALGTFLGV---VDLLFDVT 228 Query: 296 LELLIGQRKFKNKLKLDEVRLYIDGHFAEAVTLDGLARAFFVSKEYLSKVFKQEYGCNVT 355 G+R + + +V YI+ + + +TL+ A +S YLS++FK+ N Sbjct: 229 -----GERFGYSSNTVKKVLNYIERNLTKRITLEDAAEYANISPSYLSRMFKKTLNVNFI 283 Query: 356 DYILGLRMEKAKEWLADENIPIKTVAELAGYEDVGYFYRVFKKHYGVAPGEMRKQN 411 Y+ R+E AKE L PI +++ Y DV YF + FKK GV+P E R+Q+ Sbjct: 284 SYLTTRRIELAKETLIGTERPITAISQELSYSDVNYFCKSFKKEVGVSPTEYRRQS 339 >ref|NP_388577.1| similar to two-component response regulator [YesM] [Bacillus subtilis] pir||E69796 two-component response regulator [YesM] homolog yesN - Bacillus subtilis emb|CAB12515.1| yesN [Bacillus subtilis subsp. subtilis str. 168] Length = 368 Score = 123 bits (308), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 109/375 (29%), Positives = 176/375 (46%), Gaps = 37/375 (9%) Query: 61 MIKVLLVDDESWNRDIVRTFGSWEEHGMEIAGEAEDGREALRLTDELSPQIVITDMRMPG 120 M K+LL DDE D + WE G + G+A++G EA P IVITD++MPG Sbjct: 1 MYKILLADDERIILDGMAGIIEWESLGASLIGKAQNGHEAYEKIVHKQPHIVITDVKMPG 60 Query: 121 ADGVMLMNNLHARYPEIKVIVVSGYDDFKYAQNAVRYGAVDYLLKPVDPKELNAVLDKCR 180 DG+ L+ + A P ++ IV+SG+ +F+YA+ A++YG YLLKP + +++ + L++ Sbjct: 61 MDGLELIKKVSAVSPSVQFIVLSGFGEFEYAKEAMKYGVKHYLLKPCNEQQIISSLEEII 120 Query: 181 RELEEAERGQKAYA-----LDIDVSLCLSSYKQLMRVHFNELNAEGVNSVLEGIRRELEL 235 EL+ + +K A LD S Y + + +L+ L G +++ L Sbjct: 121 AELKRQDVHKKKTAHLKHELDHIRSFAADQYLEGLIAGVAQLSPP---PSLAG--KKIRL 175 Query: 236 GGAKGPGALRQAANEMLLLLKELMRGNRLEDELLKAE-----ISHEALASAANVEAYLKA 290 KG ++ AA E L G+ L E + A A V A K Sbjct: 176 LILKGEQSIDAAAREAL--------GSALTAVCSSGEWTVLAVEENAAEKVAEVFADRKM 227 Query: 291 LYGAALELLIGQRKFKNKLKLDE-----------VRLYIDGHFAEAVTLDGLAR-AFFVS 338 A EL + F++ + +RL D ++L A+ F++ Sbjct: 228 AISQAGELRHAGQLFRDTAEASGDLHGSAVISKMIRLIADELGNPNLSLKWAAKDMLFMN 287 Query: 339 KEYLSKVFKQEYGCNVTDYILGLRMEKA-KEWLADENIPIKTVAELAGYED-VGYFYRVF 396 +YL K+FKQE G + Y+ +R+E A K+ ++ + +AE G+ D YF VF Sbjct: 288 PDYLGKLFKQETGEKFSQYVTRVRLEHAMKQMKIRRDVSVSEIAEEIGFGDNPKYFSLVF 347 Query: 397 KKHYGVAPGEMRKQN 411 KK+ G+ P E R++ Sbjct: 348 KKYTGLTPSEFRRKQ 362 >ref|NP_242975.1| BH2109~unknown conserved protein [Bacillus halodurans] pir||E83913 hypothetical protein BH2109 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05828.1| BH2109~unknown conserved protein [Bacillus halodurans] Length = 525 Score = 120 bits (302), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 58/126 (46%), Positives = 84/126 (66%), Gaps = 1/126 (0%) Query: 61 MIKVLLVDDESWNRDIVRTFGSWEEHGMEIAGEAEDGREALRLTDELSPQIVITDMRMPG 120 M KVLL DDE + R +++ WE+ G E+A EA++G +A +L E+ P +VITD+RMP Sbjct: 1 MYKVLLADDEFFVRHGLKSLIEWEKCGYEVAAEADNGEDAFKLVQEIKPDLVITDIRMPV 60 Query: 121 ADGVMLMNNLHARYP-EIKVIVVSGYDDFKYAQNAVRYGAVDYLLKPVDPKELNAVLDKC 179 DG+ L+ ++ K I++SGY+DF YAQ AVR+GAVD++LKP+D EL A L K Sbjct: 61 FDGLQLIQKVNETLSFNTKFIIISGYNDFSYAQKAVRFGAVDFVLKPIDQDELEAALIKL 120 Query: 180 RRELEE 185 L++ Sbjct: 121 AATLDQ 126 Score = 67.4 bits (163), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 34/108 (31%), Positives = 61/108 (56%) Query: 303 RKFKNKLKLDEVRLYIDGHFAEAVTLDGLARAFFVSKEYLSKVFKQEYGCNVTDYILGLR 362 RK K + +V+ YI+ H+ E ++L +A F+++ Y+ ++FK+ +G +Y+L LR Sbjct: 415 RKENMKSDIYKVKTYIENHYHENISLKSIASLFYMNPVYMGQLFKKTFGVYFKEYLLKLR 474 Query: 363 MEKAKEWLADENIPIKTVAELAGYEDVGYFYRVFKKHYGVAPGEMRKQ 410 M +AK+ L ++ + VAE G+ YF F+K + P E R + Sbjct: 475 MNEAKKLLRQTDMRVYEVAERVGFGSTDYFVTQFEKINKMTPTEYRNK 522 >ref|NP_756406.1| Hypothetical protein [Escherichia coli CFT073] gb|AAN82980.1| Hypothetical protein [Escherichia coli CFT073] Length = 351 Score = 106 bits (264), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 96/365 (26%), Positives = 167/365 (45%), Gaps = 38/365 (10%) Query: 61 MIKVLLVDDESWNRDIVRTFGSWEEHGMEIAGEAEDGREALRLTDELSP-QIVITDMRMP 119 M +++V+DE + +R S I EA G++A+ L D+LS +++ D+ +P Sbjct: 1 MFTIVIVEDEPIELESLRQIISQCVENAAI-HEASTGKKAIHLIDQLSQIDMILVDINIP 59 Query: 120 GADGVMLMNNLHARYPEIKVIVVSGYDDFKYAQNAVRYGAVDYLLKPVDPKELNAVLDKC 179 +G ++ L + + K+IV++ DDF ++ DYLLKPV L + K Sbjct: 60 LPNGKQVIEYLKKKNSDTKIIVITANDDFDIVRSMYNLKVDDYLLKPVKKCILTDTIKKT 119 Query: 180 RRELEEAER------GQKAYALDIDVSLCLSSYKQLMRVHFNELN-AEGVNSVLEGIRRE 232 +E E QK +A+ D Y Q F+ LN A V+ + E R++ Sbjct: 120 L-AFDEGENEKSRALKQKVFAMIDD-----CDYTQWHNFIFDTLNGACPVDMIEEDKRKD 173 Query: 233 L--------ELGGAKGPGALRQAANEMLLLLKELMRGNRLEDELLKAEISHEALASAANV 284 + + AKG L ++ L+K++ + +E + IS ++ Sbjct: 174 VIDFLEIVSQYMTAKGE-KLMPTNRKVAALIKDINQYGMMESRYFRIMISLLTIS----- 227 Query: 285 EAYLKALYGAALELLIGQRKFKNKLKLDEVRLYIDGHFAEAVTLDGLARAFFVSKEYLSK 344 + L+ AL+ G F ++ + +I+ + +TLD +A FVS YLS+ Sbjct: 228 ----EELFDYALKGYTGNIDF-----IERAKFHIEKNILRNLTLDDVAAKSFVSACYLSR 278 Query: 345 VFKQEYGCNVTDYILGLRMEKAKEWLADENIPIKTVAELAGYEDVGYFYRVFKKHYGVAP 404 FK+ G ++YI ++ AK L ++ + T+A Y+D YF R+FKK G+ P Sbjct: 279 TFKKITGFGFSNYIATRKITIAKSLLRFSDLQVNTIALELAYQDSNYFCRIFKKDTGMTP 338 Query: 405 GEMRK 409 + RK Sbjct: 339 SDYRK 343 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.136 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 569,835,630 Number of extensions: 24443476 Number of successful extensions: 76060 Number of sequences better than 1.0e-05: 2254 Number of HSP's gapped: 78108 Number of HSP's successfully gapped: 2293 Length of query: 421 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 293 Effective length of database: 409,704,906 Effective search space: 120043537458 Effective search space used: 120043537458 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)