BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0407.0009.p (628 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_348842.1| Uncharacterized conserved protein [Clostridium ... 106 1e-21 ref|NP_782332.1| conserved protein [Clostridium tetani E88] >gi|... 72 5e-11 gb|EAH95511.1| unknown [environmental sequence] 66 3e-09 ref|NP_622724.1| conserved hypothetical protein [Thermoanaerobac... 65 6e-09 ref|NP_228544.1| conserved hypothetical protein [Thermotoga mari... 64 1e-08 >ref|NP_348842.1| Uncharacterized conserved protein [Clostridium acetobutylicum] pir||C97174 uncharacterized conserved protein CAC2225 [imported] - Clostridium acetobutylicum gb|AAK80182.1| Uncharacterized conserved protein [Clostridium acetobutylicum] Length = 643 Score = 106 bits (265), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 119/574 (20%), Positives = 244/574 (42%), Gaps = 23/574 (4%) Query: 7 ERSRYEFI--ASMLLGKTDLLNLYSVDPQTFGGTDEAKNGVIIIQNNEIFITPPLSGGKP 64 E +YE I + + K + + VD ++ K+G I I + +I + P+ GGK Sbjct: 27 EDIKYEVIEVKNGIFKKKASIAVEIVDKSENKESENKKDGTIQILDGKIVVKDPVEGGKS 86 Query: 65 AVISAIHPVVLKINGQVITGPTPITSADDL--TWEICEKPQYQITVSD--DKLKVYFTLY 120 A I + +K+N + + + + S+ ++ ++ EKP+ +I +S DK+ Y ++ Sbjct: 87 ATIVVPKNIGVKVNDKDVKDSSEVCSSSNIEVVFQEIEKPERRINISTSPDKMNAYISIS 146 Query: 121 RAERYAWNLVNNPASAFVTVRAEMNREMLLSTLSIEQIMAGFDKSPIVSSLNIPALYEEL 180 + + L + +S V + +E+ R + +I+ S + + L + Sbjct: 147 YINKKVYKLKDAESSNKVVLESEVLRVEKPPVYTQAEILDALKASGVTYGILEENLSKCN 206 Query: 181 NNPTYLPVCIAEGKAPVPGTNASLELLLQQKIENQFKSLDDSAQETGCPEFF-MVQEGEV 239 ++ + + IA+G + +E + N+F+ ++ + F V EG V Sbjct: 207 SDKEIVDMLIAQGMKVENDEDDEVEFKFDVRGNNKFQENENGKIDFKSIGFVNAVDEGSV 266 Query: 240 FAKKLPPAEGLPGFDVYGGVLPPPPPQDLTLFSSPCAALLPGGEIKACRKGRPRITGSGT 299 A + EG G D++G + +++ + + + RKG P +G+ Sbjct: 267 LAIRHIGKEGHNGKDIFGNEIKKKNGKEIKINIGEGCEFKDENTVVSTRKGEPIYSGNKI 326 Query: 300 FVKTIDFPQTFLVPPSDSEAGGTLMFPGDVIAAHDLSRNTVIEALGNVYIFGDVRNSRIA 359 V I + + + G + F GDV +S + +E+ NV I +V+N+ ++ Sbjct: 327 SVIAIHEVNSDVDLKT-----GNIKFVGDVFIHGSVSESMKVESEHNVKIDQNVQNAIVS 381 Query: 360 ATGSIFISGKVADSELYAGGYGAAHNRLCSFTELVAKEVAGLREAARHLAE-TVESTVQT 418 A+G+IFI S +Y GG + F + + L EA + + T Sbjct: 382 ASGNIFIEKNAIFSNVYGGGEDVFVLKQLEFLNTIDVNIKNLLEAVEQVKHYNMHGKAST 441 Query: 419 VKYGLVIMLLLESKYAHLPRMIRDMQELLSGMSTDYPQDTKILKHLMEFFLNPGRFTDQI 478 G ++ +LLE K+ +PR+ ++ D +++ I+ + G + + Sbjct: 442 --DGQILKVLLEGKFRLVPRLCEKFIDI-----CDREENSDIISLFKARLIGMGPLSIES 494 Query: 479 TDAGIGSFLSLLKNLAQSICQMQEERVRIDIAGSHDSIIKSGGDVHIHGDGVSRSKLHSA 538 D + SL ++ ++ M V + + +S I+S G++ I G G S L + Sbjct: 495 LDE-LTQVTSLCEDRVSNLKSMLSVPVDVSVGYCQNSKIESSGNIFITGKGEYISDLTAK 553 Query: 539 GDIYFAKNSSMSSDSTMEAAGTIHARSVGGESIG 572 ++ F ++S++ ++A + VG SIG Sbjct: 554 NNVIFTNDNSIARGGVIKAGNEVKCGVVG--SIG 585 >ref|NP_782332.1| conserved protein [Clostridium tetani E88] gb|AAO36269.1| conserved protein [Clostridium tetani E88] Length = 526 Score = 71.6 bits (174), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 108/512 (21%), Positives = 204/512 (39%), Gaps = 78/512 (15%) Query: 103 QYQITVSDDKLKVYFTLYRAERYAWNLVNNPASAFVTVRAEMNREMLLSTLS-------- 154 + I+ S D ++ + ++Y + L + +E N E++L +S Sbjct: 9 KINISTSKDNIEAFASVYYEPEIIYELKD----------SEENNELILEKVSKYSKMPPK 58 Query: 155 --IEQIMAGFDKSPIVSSLNIPALYEELNN---PTYLPVCIAEGKAPVPGTNASLELLLQ 209 +E+I K I+ + + E L N + V IA+G P+ + +E Sbjct: 59 YTVEEIKEQLSKGGIICGI----IEENLQNVIEKSCDNVLIAKGVRPIHEKDDVIEYRFN 114 Query: 210 QKIENQFKSLDDSA------QETGCPEFFMVQEGEVFAKKLPPAEGLPGFDVYGGVLPPP 263 ++ + K L++ A + G E V++G++ A + G G DV G +L Sbjct: 115 --VDEREKKLEEDAMGNVDFKSIGTVE--AVKKGDILAIRHSSKPGRDGVDVKGKLLKHK 170 Query: 264 PPQDLTLFSSPCAALLPGGEIKACRKGRPRITGSGTFVKTIDFPQTFLVPPSDSEAGGTL 323 + + + + L G +I A +G+P + + +V + + V G + Sbjct: 171 LGKKIRIRAGN-GCQLSGEKIIASIEGKPYVKNNAFYV-----YRMYEVRSDVDLTTGNI 224 Query: 324 MFPGDVIAAHDLSRNTVIEALGNVYIFGDVRNSRIAATGSIFISGKVADSELYAGGYGAA 383 F GDV+ ++ + A ++ I +V S+I G+I I G + S++ GG Sbjct: 225 EFIGDVLVYGNVKEGMEVNAGNSITINKNVERSKIEGKGNITIKGSILSSDILGGGQDVT 284 Query: 384 HNRLCSFTELVAKEVAGLREAARHLAETVESTVQTV---------KYGLVIMLLLESKYA 434 K + + L E +EST + K G +I +L+E KY Sbjct: 285 K----------IKAIDNSKALINLLREIIESTKEVKRLDLYGKDKKDGEIIRILMERKYK 334 Query: 435 HLPRMIRDMQELLSGMSTDYPQDTKILKHLMEFF----LNPGRFTDQITDAGIGSFLSLL 490 LPR ++ ++ D + IL + LN + + ++ Sbjct: 335 TLPRTCLNIIRDITVCGKDQEEGKIILAVKNKLIGLAPLNIKNYRE---------LEDII 385 Query: 491 KNLAQSICQMQEER---VRIDIAGSHDSIIKSGGDVHIHGDGVSRSKLHSAGDIYFAKNS 547 K L + + ++++E + + I DS I S G+V+I G G S + S IYF Sbjct: 386 KLLEERVVKLEKELTIPIDVIIDYCQDSNISSSGNVYITGKGEYISNIVSNNSIYFTSEK 445 Query: 548 SMSSDSTMEAAGTIHARSVGGESIGKSLLVAG 579 ++ ++A I ++VG S + L+ G Sbjct: 446 GLARGGQIKAKKEIRCKTVGSISGVTTKLIVG 477 >gb|EAH95511.1| unknown [environmental sequence] Length = 563 Score = 65.9 bits (159), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 66/287 (22%), Positives = 127/287 (44%), Gaps = 35/287 (12%) Query: 139 TVRAEMNREMLLSTLSIE-----------QIMAGFDKSPI---VSSLNIPALYEELNNPT 184 +V+ E++ + +L+T+++ +I+ K+ + +S L I L +++ Sbjct: 83 SVKIEISPDKMLATMTLTAPCGGKAVDLPEILTELKKNNVCMGLSKLKIQTLLQKITKLK 142 Query: 185 YLPVC---IAEGKAPVPGTNASLEL---LLQQKIENQFKSLDDSAQETGCPEFFMVQEGE 238 C IA+GK PV G NA LE L ++++ + D + MV+ + Sbjct: 143 PGDSCQSEIAQGKHPVNGQNAVLERKVSLARERLLQPQEREDGTVDMRNLGSMIMVKPND 202 Query: 239 VFAKKLPPAEGLPGFDVYGGVLPPPPPQDLTLFSSPCAALLPG--GEIKACRKGRPRITG 296 + K P EG PG+++ G VL P +DL L + ++ P ++ A G+P T Sbjct: 203 ILMIKHPGTEGSPGYNIKGEVLKQKPGKDLPLLAGTGTSIHPNDPNKLIATVAGQPVETR 262 Query: 297 SGTFVKTIDFPQTFLVPPSDSEAGGTLMFPGDVIAAHDLSRNTVIEALGNVYIFGDVRNS 356 +G V D Q V S G + F G ++ D+ ++++ G++ + G V ++ Sbjct: 263 TGMNVD--DVLQLKDVDIST----GHVTFKGSILITGDVHEGMIVKSSGDITVMGFVDSA 316 Query: 357 RIAATGSIFISGKVADSELYAGGY-------GAAHNRLCSFTELVAK 396 + A G I +S V ++ + G + +++L AK Sbjct: 317 TLEAEGDITVSKGVIGRQIRVNEFSTNLTAQGQISAQFVQYSQLTAK 363 >ref|NP_622724.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] gb|AAM24328.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] Length = 502 Score = 64.7 bits (156), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 105/465 (22%), Positives = 181/465 (38%), Gaps = 75/465 (16%) Query: 151 STLSIEQIMAGFDKSPIVSSLNIPALYEELNNPTYLPVCIAEGKAPVPGTNASLELLLQ- 209 S L I+ G DKS ++S +Y + IA G G + LE +++ Sbjct: 38 SELRKNGIIVGIDKSALIS------VYTQNKKEA----VIARGIPYKSGRDGYLEFIIKI 87 Query: 210 ---QKIENQFKSLDDSAQETGCPEFFMVQEGEVFAKKLPPAEGLPGFDVYGGVLPPPPPQ 266 + + + S +D P ++G+V K P +G G V G ++ PP + Sbjct: 88 DKPRILYTETFSEEDIFDMASIP---FARKGDVIGKLHKPIKGENGISVRGRIINAPPVR 144 Query: 267 DL--TLFSSPCAALLPGGEIKACRKGRPRITG---SGTFVKTIDFPQTFLVPPSDSEAGG 321 D+ + S +I A RP + + FV F S + Sbjct: 145 DVEVKILSDSIVFKEDEDKIVAIADIRPVVHKKDETDKFVYLFYSIPVFFYKGSIPKNSR 204 Query: 322 TLMFPGDVIAAHDLSRNTVIEALGNVYIFGDVRNSRIAATGSIFISGKVADSELYAGGY- 380 + F GDVI ++ + I A GNV I G V S + +I ISG + DS+L+AG Sbjct: 205 LVTFEGDVIINGNVEKGNQIIASGNVQILGSVYESVVEGGQNIIISGGIVDSQLHAGFLP 264 Query: 381 GAAHNRL-----------CSFTELVAKEVAGLREAARHLAETVESTVQTVKYGLVIMLLL 429 G +++ S L +E+ + +RHL+E + Sbjct: 265 GNIFDKIELHAVNLIVEKLSAIFLHIRELPTVMNNSRHLSEKI----------------- 307 Query: 430 ESKYAHLPRMIRDMQELLSGMSTDYP--QDTKILKHLMEFFLNPGRFTDQITD---AGIG 484 ++I ++E L ST+ + + +L++L+E + + + I D G+ Sbjct: 308 --------KLIETLKEELKTSSTEISMIRSSNVLQNLLEDLKSLVKTLNNILDYDIIGLI 359 Query: 485 SFLSLLKNLAQSIC-------QMQEERVRIDIAGSHDSIIKSGGDVHIHGD-GVSRSKLH 536 L+ LK + + I + E + I HDS I + DV I + G +K+ Sbjct: 360 KHLNSLKEIYEKILFISRILTEEHENKGHILAKWVHDSKIYAMKDVIITSEAGCYNTKIS 419 Query: 537 SAGDIYFAKNSSMSSDSTMEAAGTIHARSVGGESIGKSLLVAGRQ 581 + G + M ST+E I + G +G +L+ G + Sbjct: 420 TNGSVSINGKVRM---STIEFKKNIFIKEAGSLGVGSRVLLKGSK 461 >ref|NP_228544.1| conserved hypothetical protein [Thermotoga maritima] pir||B72339 conserved hypothetical protein - Thermotoga maritima (strain MSB8) gb|AAD35816.1| conserved hypothetical protein [Thermotoga maritima] Length = 455 Score = 63.5 bits (153), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 74/271 (27%), Positives = 120/271 (44%), Gaps = 39/271 (14%) Query: 103 QYQITVSDDKLKVYFTLYRAERYAWNLVNNPASAFVTVRAEMNREMLLSTLSIEQIMAGF 162 + +ITVS+D+++ Y TL + L +E LL+ L I G Sbjct: 2 KVEITVSEDRMQAYVTLRKEGSSELPL---------------TKEELLNALLSAGINHGI 46 Query: 163 DKSPIVSSLNIPALYEELNNPTY-LPVCIAEGKAPVPGTNASLELLLQQKIE---NQFKS 218 K I+ L +P Y PV +AEG PV G + +ELL +KIE ++ + Sbjct: 47 -KEEIIEDL--------ARSPVYNTPVLVAEGTPPVDGEDGRVELL--KKIEFSSHEGEK 95 Query: 219 LDDSAQETGCPEFFMVQEGEVFAKKLPPAEGLPGFDVYGGVLPPPPPQDLTLFSSPCAAL 278 +D +E + +V++GE A+ +PP G G DV+G + P P + + + Sbjct: 96 ID--LREIPAKQRVIVRKGETIARIIPPTPGKEGMDVFGNPVKPKPGR-VPEYHLGYNVA 152 Query: 279 LPGGEIKACRKGRPRITGSGTFVKTIDFPQTFLVPPSDSEAGGTLMFPGDVIAAHDLSRN 338 EI A + G I +G TI T V D + G + FPG V+ D+ + Sbjct: 153 TKNNEIIATKSGILVIEPNG----TIHVYDTLEVDNVDY-STGNIDFPGKVVVKGDVKPD 207 Query: 339 TVIEALGNVYIFGDVRNSR-IAATGSIFISG 368 V++A ++ + G + + I+ GSI G Sbjct: 208 FVVKAQEDITVKGVIEAATVISFNGSITAGG 238 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 934,628,440 Number of extensions: 40850114 Number of successful extensions: 106270 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 106257 Number of HSP's successfully gapped: 20 Length of query: 628 Length of database: 757,237,578 Length adjustment: 132 Effective length of query: 496 Effective length of database: 398,844,510 Effective search space: 197826876960 Effective search space used: 197826876960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)