BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0402.0039.p (312 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_466322.1| similar to dehydrogenase [Listeria monocytogene... 77 4e-13 ref|NP_472259.1| similar to dehydrogenase [Listeria innocua] >gi... 77 6e-13 gb|EAH47754.1| unknown [environmental sequence] 77 6e-13 ref|NP_619309.1| myo-inositol 2-dehydrogenase [Methanosarcina ac... 72 2e-11 ref|XP_306855.1| ENSANGP00000000107 [Anopheles gambiae] >gi|2128... 69 1e-10 >ref|NP_466322.1| similar to dehydrogenase [Listeria monocytogenes EGD-e] pir||AG1424 dehydrogenase homolog lmo2800 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAD01013.1| lmo2800 [Listeria monocytogenes] Length = 333 Score = 77.4 bits (189), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 42/195 (21%), Positives = 90/195 (46%), Gaps = 5/195 (2%) Query: 1 MNIGILGTG-FGAYHASLLAKMEGVTRIVIFGRNEAKLGKLKEELGVXXXXXXXXXXXXX 59 + +G++G G G YHA + K+ V + I N+ + ++ E+ Sbjct: 5 IKVGVIGVGQMGQYHAEIYQKLPQVELVAICEYNDERRAEMAEKFQCKAYKDYHELLANT 64 Query: 60 XXXXXXXXXXSHLHSQYAIQALKAGKHVFCETPVCLSRADALALRQAEERYGQRIMVNQF 119 ++H +A+K KH+ E P+ D A+ + + Y + V Sbjct: 65 EIEAVSIVLPDNMHRDCVEEAVKYKKHILLEKPLAKELEDGKAMYELTKDYDKVFTVGFL 124 Query: 120 IKFDHAYTRLYESVNSGQYGKLLHVTLKRETAPLWGD---LGLSKIAANLMIHELDMVTW 176 ++FD + + + +++G+ G ++H+ +R +P+ G +G S ++ ++MIH++D + W Sbjct: 125 LRFDPRFNMIKQRLDNGELGDIIHLYCRR-NSPITGPKRYIGASDLSMHVMIHDIDYINW 183 Query: 177 LLGNPPISSIWGSSS 191 + + P+ I S S Sbjct: 184 YMDSEPVKVIAKSRS 198 >ref|NP_472259.1| similar to dehydrogenase [Listeria innocua] pir||AE1798 dehydrogenase homolog lin2932 [imported] - Listeria innocua (strain Clip11262) emb|CAC98157.1| lin2932 [Listeria innocua] Length = 333 Score = 77.0 bits (188), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 42/195 (21%), Positives = 90/195 (46%), Gaps = 5/195 (2%) Query: 1 MNIGILGTG-FGAYHASLLAKMEGVTRIVIFGRNEAKLGKLKEELGVXXXXXXXXXXXXX 59 + +G++G G G YHA + K+ V + I N+ + ++ E+ Sbjct: 5 IKVGVIGVGQMGQYHAEIYQKLPQVELVAICEFNDERRAEMAEKFQCKAYKDYHELLANT 64 Query: 60 XXXXXXXXXXSHLHSQYAIQALKAGKHVFCETPVCLSRADALALRQAEERYGQRIMVNQF 119 ++H +A+K KH+ E P+ D A+ + + Y + V Sbjct: 65 EIEAVIIVLPDNMHRDCVEEAVKHKKHILLEKPLAKELEDGKAMYELTKDYDKVFTVGFL 124 Query: 120 IKFDHAYTRLYESVNSGQYGKLLHVTLKRETAPLWGD---LGLSKIAANLMIHELDMVTW 176 ++FD + + + +++G+ G ++H+ +R +P+ G +G S ++ ++MIH++D + W Sbjct: 125 LRFDPRFNMIKQRLDNGELGDIIHLYCRR-NSPITGPKRYIGASDLSMHVMIHDIDYINW 183 Query: 177 LLGNPPISSIWGSSS 191 + + P+ I S S Sbjct: 184 YMDSEPVKVIAKSRS 198 >gb|EAH47754.1| unknown [environmental sequence] Length = 302 Score = 76.6 bits (187), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 58/176 (32%), Positives = 84/176 (47%), Gaps = 10/176 (5%) Query: 73 HSQYAIQALKAGKHVFCETPVCLSRADALALRQAEERYGQRIMVNQFIKFDHAYTRLYES 132 H AI+AL+AGKHV E P+ LS DA + + E+ G+++MV + F Y L Sbjct: 66 HRDAAIKALRAGKHVLVEKPIALSLRDADRMIREAEKAGRQLMVAHVLPFFPEYAYLASC 125 Query: 133 VNSGQYGKLLHVTLKRETA-PLW-GDL-GLSKIAA---NLMIHELDMVTWLLGNPPISSI 186 V SG++G LL KR + P W DL L + +L IH+ + + G P S Sbjct: 126 VASGEHGALLGAHFKRIISQPDWSADLTNLERSGGPGVDLHIHDTHFIAAVCGKPDAVSS 185 Query: 187 WGSSSKDGQALVHAD---FDT-PQFSAEILVSSQMPEAYPFTVGYEAYFEQAKLVF 238 G +D A A FD+ P S + + PF G+EAYFE +++ Sbjct: 186 TGRLVEDRYAAYLATAYRFDSRPDLSVTCTGGAISQKGRPFVHGFEAYFESGTVLY 241 >ref|NP_619309.1| myo-inositol 2-dehydrogenase [Methanosarcina acetivorans str. C2A] gb|AAM07789.1| myo-inositol 2-dehydrogenase [Methanosarcina acetivorans str. C2A] Length = 311 Score = 71.6 bits (174), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 48/196 (24%), Positives = 93/196 (47%), Gaps = 7/196 (3%) Query: 1 MNIGILGTGF-GAYHASLLAKMEGVTRIVIFGRNEAKLGKLKEELGVXXXXXXXXXXXXX 59 + +G++GTG+ G H + ++MEGV I I N +++ L G Sbjct: 2 IRVGVVGTGYMGENHVRIYSEMEGVKLIGISDPNTSRVKGLAVRYGTLPFADHKKMFEKT 61 Query: 60 XXXXXXXXXXSHLHSQYAIQALKAGKHVFCETPVCLSRADALALRQAEERYGQRIMVNQF 119 + LH + AL+AG V E P+ S +A A+ ++ E + +MV Sbjct: 62 KLDAISIAAPTTLHCPIVLDALRAGVDVLVEKPIADSVENATAMIESAEEMDRHLMVGHI 121 Query: 120 IKFDHAYTRLYESVNSGQYGKLLHVTLKR--ETAPLWGDLGLSKIAANLMIHELDMVTWL 177 +F+ A +L E ++SG GK++ V+ +R P D+G + ++ +H++D+++ + Sbjct: 122 ERFNPAVLKLKEIIDSGTLGKVVSVSTRRVGPYNPRIRDVG---VILDIGVHDIDIISCI 178 Query: 178 LGNPPISSIWGSSSKD 193 G I+ ++ + D Sbjct: 179 YGM-KINQVYAVAGSD 193 >ref|XP_306855.1| ENSANGP00000000107 [Anopheles gambiae] gb|EAA01949.1| ENSANGP00000000107 [Anopheles gambiae str. PEST] Length = 277 Score = 69.3 bits (168), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 39/132 (29%), Positives = 75/132 (56%), Gaps = 9/132 (6%) Query: 73 HSQYAIQALKAGKHVFCETPVCLSRADALALRQAEERYGQRIMVNQFIK-FDHAYTRLYE 131 H++Y ++A++ K+VFCE P+ S D L + +AE +G+R++ F++ +D Y L Sbjct: 19 HARYVLEAIRHKKYVFCEKPLATSAEDCLKIVEAEAAHGKRLVQVGFMRPYDTGYRALKA 78 Query: 132 SVNSGQYGKLLHVTL----KRETAPLWGDLGLSKIAANLMIHELDMVTWLLGNPPISSIW 187 ++ G+ G++L + +R A D+G++ + +IHELD+ W+LG+ +S+ Sbjct: 79 LIDKGEIGEVLMIHAAHRNQRVGANYKTDMGIT----DTLIHELDVFRWMLGDDYVSAQV 134 Query: 188 GSSSKDGQALVH 199 G+A+ H Sbjct: 135 IFPRSSGKAMEH 146 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 404,445,764 Number of extensions: 14958074 Number of successful extensions: 31337 Number of sequences better than 1.0e-05: 74 Number of HSP's gapped: 31500 Number of HSP's successfully gapped: 74 Length of query: 312 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 187 Effective length of database: 417,850,203 Effective search space: 78137987961 Effective search space used: 78137987961 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)