BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0402.0018.p (115 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00078345.1| hypothetical protein [Methanosarcina barkeri] 61 7e-09 ref|NP_633896.1| conserved protein [Methanosarcina mazei Goe1] >... 53 2e-06 >ref|ZP_00078345.1| hypothetical protein [Methanosarcina barkeri] Length = 112 Score = 61.2 bits (147), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 35/101 (34%), Positives = 56/101 (55%), Gaps = 2/101 (1%) Query: 10 DIEFSAIEVGRDLLLVIGGGVRHIGAASTAYMEEGNVKVSTSAV--PHHKEHTVSESIAT 67 +I A +VG D LL + GG H+GA + +E N + S+S + P H+E ++ A Sbjct: 5 EIVLDAKKVGEDYLLTLTGGEEHVGAVAVGLFDEKNQRASSSVITMPGHREEYLALDGAR 64 Query: 68 RVAEALDRTVTVVMGIHYDNLSKEGIARVVEIVNLKVDQYL 108 +V+ A +T V+GIH DN+S E I +V V V+ ++ Sbjct: 65 QVSRATKKTSVFVVGIHQDNISPEEIKDIVSAVGEMVESFI 105 >ref|NP_633896.1| conserved protein [Methanosarcina mazei Goe1] gb|AAM31568.1| conserved protein [Methanosarcina mazei Goe1] Length = 126 Score = 53.1 bits (126), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 32/101 (31%), Positives = 53/101 (52%), Gaps = 2/101 (1%) Query: 10 DIEFSAIEVGRDLLLVIGGGVRHIGAASTAYMEEGNVKVSTSAV--PHHKEHTVSESIAT 67 +I ++G D LL + GG H GA + +E + + S+S + P H+E ++ A Sbjct: 12 EITLEVKKLGEDYLLTLTGGKDHAGAVAVGLFDEKSGRASSSVITLPGHREEQLALDSAR 71 Query: 68 RVAEALDRTVTVVMGIHYDNLSKEGIARVVEIVNLKVDQYL 108 RV++A +T VV GIH D++S E I +V V ++ Sbjct: 72 RVSKATGKTSIVVAGIHVDSISLEEIKEIVSTAEEMVGNFI 112 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.132 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 140,961,305 Number of extensions: 4421777 Number of successful extensions: 15061 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 15061 Number of HSP's successfully gapped: 3 Length of query: 115 Length of database: 757,237,578 Length adjustment: 80 Effective length of query: 35 Effective length of database: 540,029,658 Effective search space: 18901038030 Effective search space used: 18901038030 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 120 (50.8 bits)