BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0400.0001.p (123 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_267692.1| hypothetical protein L178933 [Lactococcus lacti... 168 4e-41 ref|ZP_00062701.1| COG0599: Uncharacterized homolog of gamma-car... 167 9e-41 ref|ZP_00037149.1| COG0599: Uncharacterized homolog of gamma-car... 160 6e-39 ref|NP_600274.1| hypothetical protein NCgl1004 [Corynebacterium ... 149 2e-35 ref|NP_687459.1| carboxymuconolactone decarboxylase family prote... 147 1e-34 >ref|NP_267692.1| hypothetical protein L178933 [Lactococcus lactis subsp. lactis] pir||H86816 hypothetical protein yphJ [imported] - Lactococcus lactis subsp. lactis (strain IL1403) gb|AAK05634.1| HYPOTHETICAL PROTEIN [Lactococcus lactis subsp. lactis] Length = 102 Score = 168 bits (425), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 78/102 (76%), Positives = 89/102 (87%) Query: 19 LEKQTAGRDNLGEFAPKFAELNDDVLFGEVWSREKELSPRDRSMITVATLISEGNFEQLT 78 +EKQTAG + LGEFAPKFAELNDD+LFGEVWSREKELS +RS+IT+ LI+ GNFEQL Sbjct: 1 MEKQTAGHEQLGEFAPKFAELNDDILFGEVWSREKELSAHERSVITITALIAGGNFEQLN 60 Query: 79 PHLHKAKANGITQEEIAEIITHLSFYAGWPKAWSAFNIAKQI 120 H+ K K NGI+QEEI+EIITHLSFY GWPKAWSAFNIAK+I Sbjct: 61 FHMAKGKENGISQEEISEIITHLSFYVGWPKAWSAFNIAKKI 102 >ref|ZP_00062701.1| COG0599: Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit [Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293] Length = 156 Score = 167 bits (422), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 77/104 (74%), Positives = 88/104 (84%) Query: 19 LEKQTAGRDNLGEFAPKFAELNDDVLFGEVWSREKELSPRDRSMITVATLISEGNFEQLT 78 +EKQTAG D LGEFAPKFAELNDDVLFGEVWSRE ELS DRS+IT++ LIS GN EQ Sbjct: 53 VEKQTAGHDKLGEFAPKFAELNDDVLFGEVWSRESELSAHDRSIITISALISGGNMEQAG 112 Query: 79 PHLHKAKANGITQEEIAEIITHLSFYAGWPKAWSAFNIAKQIFN 122 H+ K NGITQ+EI+E+ITHL+FY GWPKAWSAFNIAK+I+N Sbjct: 113 HHMTLGKKNGITQKEISEMITHLAFYVGWPKAWSAFNIAKEIWN 156 >ref|ZP_00037149.1| COG0599: Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit [Enterococcus faecium] Length = 114 Score = 160 bits (406), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 77/102 (75%), Positives = 87/102 (85%), Gaps = 1/102 (0%) Query: 20 EKQTAGRDNLGEFAPKFAELNDDVLFGEVWSREKELSPRDRSMITVATLISEGNFEQLTP 79 EKQTAGRD LG+FAPKFAELNDDVLFG+VWSRE ELS RS+IT++ LIS GNFEQL Sbjct: 3 EKQTAGRDQLGDFAPKFAELNDDVLFGQVWSRE-ELSAHQRSIITISALISGGNFEQLPA 61 Query: 80 HLHKAKANGITQEEIAEIITHLSFYAGWPKAWSAFNIAKQIF 121 HL K NGIT EEIAEIITHL+FY GWPKAWSAF++AK+I+ Sbjct: 62 HLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEIY 103 >ref|NP_600274.1| hypothetical protein NCgl1004 [Corynebacterium glutamicum ATCC 13032] dbj|BAB98442.1| Uncharacterized ACR, homolog of gamma-carboxymuconolactone decarboxylase subunit [Corynebacterium glutamicum ATCC 13032] emb|CAF19753.1| Carboxymuconolactone decarboxylase [Corynebacterium glutamicum ATCC 13032] Length = 107 Score = 149 bits (376), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 69/104 (66%), Positives = 83/104 (79%), Gaps = 1/104 (0%) Query: 20 EKQTAGRDNLGEFAPKFAELNDDVLFGEVWSREKELSPRDRSMITVATLISEGNFEQ-LT 78 +K TAGRD LGEFAPK AELNDDVLFG+VWSRE ELSPRDRS++ V L++ G + Sbjct: 3 QKVTAGRDILGEFAPKSAELNDDVLFGQVWSRESELSPRDRSIVIVTMLMASGVLDSAFE 62 Query: 79 PHLHKAKANGITQEEIAEIITHLSFYAGWPKAWSAFNIAKQIFN 122 H+ +AK NG+T EEIAEIITH++FYAGWPKAW+AF IAK I+ Sbjct: 63 SHVQRAKDNGVTAEEIAEIITHVAFYAGWPKAWAAFRIAKDIYT 106 >ref|NP_687459.1| carboxymuconolactone decarboxylase family protein [Streptococcus agalactiae 2603V/R] ref|NP_734928.1| Unknown [Streptococcus agalactiae NEM316] gb|AAM99331.1| carboxymuconolactone decarboxylase family protein [Streptococcus agalactiae 2603V/R] emb|CAD46104.1| Unknown [Streptococcus agalactiae NEM316] Length = 105 Score = 147 bits (370), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 68/102 (66%), Positives = 83/102 (81%) Query: 20 EKQTAGRDNLGEFAPKFAELNDDVLFGEVWSREKELSPRDRSMITVATLISEGNFEQLTP 79 EKQTAGR L EFAP+FA NDD+LFGEVW++E L+ + RS+IT++ LIS GN EQL Sbjct: 3 EKQTAGRRQLEEFAPEFARYNDDILFGEVWAKEDHLTDKTRSIITISALISGGNLEQLEH 62 Query: 80 HLHKAKANGITQEEIAEIITHLSFYAGWPKAWSAFNIAKQIF 121 HL AK NG+T+EEIA+IITHL+FY GWPKAWSAFN AK+I+ Sbjct: 63 HLQFAKQNGVTKEEIADIITHLAFYVGWPKAWSAFNKAKEIW 104 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.134 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 202,556,911 Number of extensions: 7851030 Number of successful extensions: 17567 Number of sequences better than 1.0e-05: 125 Number of HSP's gapped: 17522 Number of HSP's successfully gapped: 134 Length of query: 123 Length of database: 757,237,578 Length adjustment: 87 Effective length of query: 36 Effective length of database: 521,023,965 Effective search space: 18756862740 Effective search space used: 18756862740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 120 (50.8 bits)