BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0397.0040.p (235 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_987311.1| Conserved hypothetical protein [Methanococcus m... 310 1e-83 ref|NP_832616.1| Alpha/beta hydrolase [Bacillus cereus ATCC 1457... 299 4e-80 ref|NP_979199.1| conserved hypothetical protein [Bacillus cereus... 296 2e-79 ref|NP_845206.1| conserved hypothetical protein [Bacillus anthra... 291 8e-78 ref|ZP_00077650.1| COG1073: Hydrolases of the alpha/beta superfa... 287 1e-76 >ref|NP_987311.1| Conserved hypothetical protein [Methanococcus maripaludis S2] emb|CAF29747.1| Conserved hypothetical protein [Methanococcus maripaludis] Length = 234 Score = 310 bits (795), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 134/233 (57%), Positives = 181/233 (77%) Query: 2 MVKQEFRIEGIPAILWGDQSDKLFVAIHGNMSNKADDAIVIFAGEAVRRGYQVISFDLPE 61 M+K +I+GIPAILWG + +++F+A+HGNMS+K D I + + AV++GYQV+SFDLPE Sbjct: 1 MIKNNVKIDGIPAILWGKEQNRVFIAVHGNMSSKDDTVIQMLSEIAVKKGYQVLSFDLPE 60 Query: 62 HGDRKEEAYSCKVQNCVHDLDVIMRYAQRLSDDISIFACSMGVYFSLLAYRDLPLKQCLF 121 GDRK + CKV CV+DL+ IM+YA+ + IS+FACSMG YFSLLA+ D L++ LF Sbjct: 61 QGDRKNDKTPCKVDICVNDLEKIMKYAKNRWNRISLFACSMGAYFSLLAFADENLEKSLF 120 Query: 122 LSPVLDMKRIIENMMAWFGISEDRLKAEQEVATPVGQTLYWDYYCYVNSHPVDAWHKPTS 181 LSPV+DMKRII+NMM WF ++ +RL+ E+E+ TP+GQ LYWDY+CYVN HP+ +W+ TS Sbjct: 121 LSPVVDMKRIIQNMMNWFDVTSERLEKEKEIETPIGQKLYWDYFCYVNEHPIKSWNFKTS 180 Query: 182 ILYGSDDNLCEFDVVSAFASRFHCGLKVMEQGEHYFHTKEQLQFFRQWLKEQI 234 ILYGS D LCE+D V+ F +F C L++++ GEHYFHTKEQL F WLK++I Sbjct: 181 ILYGSKDELCEYDTVNNFVKQFSCNLEIVKSGEHYFHTKEQLTSFENWLKKEI 233 >ref|NP_832616.1| Alpha/beta hydrolase [Bacillus cereus ATCC 14579] gb|AAP09817.1| Alpha/beta hydrolase [Bacillus cereus ATCC 14579] Length = 234 Score = 299 bits (765), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 129/233 (55%), Positives = 175/233 (75%) Query: 2 MVKQEFRIEGIPAILWGDQSDKLFVAIHGNMSNKADDAIVIFAGEAVRRGYQVISFDLPE 61 M+K F+I IPA+LWGD+S+K+F+A+HGNMSNK D I I A EA R+GYQV+SFDLPE Sbjct: 1 MLKNIFKISNIPAVLWGDKSEKIFIAVHGNMSNKEDAVIQIVAEEADRKGYQVLSFDLPE 60 Query: 62 HGDRKEEAYSCKVQNCVHDLDVIMRYAQRLSDDISIFACSMGVYFSLLAYRDLPLKQCLF 121 HG+RK + CKVQ CV++L +IM YA+ +I++FACSMG YFSLLA+++ +++ LF Sbjct: 61 HGERKNDNTPCKVQFCVNELSIIMNYAKEHWKEINVFACSMGAYFSLLAFQNDVIEKALF 120 Query: 122 LSPVLDMKRIIENMMAWFGISEDRLKAEQEVATPVGQTLYWDYYCYVNSHPVDAWHKPTS 181 LSPV++M+RIIENMM WF ++ + L+ E + TP+GQ LYWDY CYV HP+ W+ T Sbjct: 121 LSPVVNMERIIENMMKWFNVTPELLQKEMTIETPIGQKLYWDYLCYVKEHPISTWNTDTY 180 Query: 182 ILYGSDDNLCEFDVVSAFASRFHCGLKVMEQGEHYFHTKEQLQFFRQWLKEQI 234 I+YG+ D LCEF+ ++ F + C L+VME GEHY HT+EQL+ F QWL + I Sbjct: 181 IMYGAKDELCEFETINYFTKKHRCELEVMETGEHYLHTEEQLKIFEQWLHKHI 233 >ref|NP_979199.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] gb|AAS41807.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] Length = 234 Score = 296 bits (759), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 125/234 (53%), Positives = 173/234 (73%) Query: 2 MVKQEFRIEGIPAILWGDQSDKLFVAIHGNMSNKADDAIVIFAGEAVRRGYQVISFDLPE 61 M+K F+I IP +LWGD+S+K+F+A+HGN+SNK D I I A EA ++GYQV+SFDLPE Sbjct: 1 MLKNNFKISNIPVVLWGDKSEKIFIAVHGNISNKEDTVIQILAEEANQKGYQVLSFDLPE 60 Query: 62 HGDRKEEAYSCKVQNCVHDLDVIMRYAQRLSDDISIFACSMGVYFSLLAYRDLPLKQCLF 121 HG+R + CKVQ C +L +IM YA+ ++S+FACS+G YFSLLAY++ +++ LF Sbjct: 61 HGERTNDNTPCKVQVCASELSIIMNYAKEHWKEVSVFACSIGAYFSLLAYQNDVIEKALF 120 Query: 122 LSPVLDMKRIIENMMAWFGISEDRLKAEQEVATPVGQTLYWDYYCYVNSHPVDAWHKPTS 181 LSPV++M+RIIENMM WF ++ + L+ E + TP+GQ LYWDY CYV HP++ W+ T Sbjct: 121 LSPVVNMERIIENMMKWFNVTPELLQKEMTIETPIGQKLYWDYLCYVKEHPINTWNTDTY 180 Query: 182 ILYGSDDNLCEFDVVSAFASRFHCGLKVMEQGEHYFHTKEQLQFFRQWLKEQIG 235 I+YG+ D LCEF +++ F + C L+VME GEHYFHT EQL+ F+QWL + I Sbjct: 181 IMYGAKDELCEFKIINDFTKKHRCELEVMETGEHYFHTAEQLKIFQQWLHKHIN 234 >ref|NP_845206.1| conserved hypothetical protein [Bacillus anthracis str. Ames] gb|AAP26692.1| conserved hypothetical protein [Bacillus anthracis str. Ames] Length = 251 Score = 291 bits (745), Expect = 8e-78, Method: Compositional matrix adjust. Identities = 126/233 (54%), Positives = 172/233 (73%) Query: 2 MVKQEFRIEGIPAILWGDQSDKLFVAIHGNMSNKADDAIVIFAGEAVRRGYQVISFDLPE 61 M+K F I IPA+LWGD+S+K+F+A+HGNMSNK D I A EA ++GYQV+SFDLPE Sbjct: 18 MLKDNFNISNIPAVLWGDRSEKVFIAVHGNMSNKEDTVIQKLAEEANQKGYQVLSFDLPE 77 Query: 62 HGDRKEEAYSCKVQNCVHDLDVIMRYAQRLSDDISIFACSMGVYFSLLAYRDLPLKQCLF 121 HG+R + CKVQ CV +L +IM YA+ +S+FACS+G YFSLLAY++ +++ LF Sbjct: 78 HGERTNDNTPCKVQVCVSELSIIMNYAKEHWKQVSVFACSIGAYFSLLAYQNDVIEKALF 137 Query: 122 LSPVLDMKRIIENMMAWFGISEDRLKAEQEVATPVGQTLYWDYYCYVNSHPVDAWHKPTS 181 LSPV++M+RIIENMM WF ++ + L+ E + TP+GQ LYWDY CYV HP++ W+ T Sbjct: 138 LSPVVNMERIIENMMKWFNVTPELLQKEMTIETPIGQKLYWDYLCYVKEHPINTWNIDTY 197 Query: 182 ILYGSDDNLCEFDVVSAFASRFHCGLKVMEQGEHYFHTKEQLQFFRQWLKEQI 234 I+YG+ D LCEF++++ F + C L+VME GEHYFHT+EQL+ F WL + I Sbjct: 198 IMYGAKDELCEFEIINDFTKKHRCALEVMETGEHYFHTEEQLKIFELWLHKII 250 >ref|ZP_00077650.1| COG1073: Hydrolases of the alpha/beta superfamily [Methanosarcina barkeri] Length = 239 Score = 287 bits (734), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 136/238 (57%), Positives = 171/238 (71%), Gaps = 5/238 (2%) Query: 2 MVKQEFRIEG----IPAILWGDQSDKLFVAIHGNMSNKADDAIVIFAGEAVRRGYQVISF 57 M K+ F I IPAILWG QS+KL + +HGN+SNK D I + A +AV +GYQ +SF Sbjct: 1 MEKRHFYISNNKIRIPAILWGRQSEKLLIEVHGNLSNKEDTVISMMAQKAVEKGYQALSF 60 Query: 58 DLPEHGDRKEEAYSCKVQNCVHDLDVIMRYAQRLSDDISIFACSMGVYFSLLAYRDLPLK 117 DLP HG+R +E Y+C +NCV DL + YA+ L+ DI +FACSMG YFSLLAY DL +K Sbjct: 61 DLPMHGERLDEEYACIPENCVSDLAAVYEYAKSLAPDIYLFACSMGAYFSLLAYHDLNIK 120 Query: 118 QCLFLSPVLDMKRIIENMMAWFGISEDRLKAEQEVATPVGQTLYWDYYCYVNSHPV-DAW 176 Q LFLSPV++M+RII NMM F +SE+RLKAE E+ P+GQTL W YYCYV +P+ W Sbjct: 121 QSLFLSPVVNMERIIHNMMEGFQVSEERLKAEHEIRLPIGQTLEWSYYCYVKENPICFEW 180 Query: 177 HKPTSILYGSDDNLCEFDVVSAFASRFHCGLKVMEQGEHYFHTKEQLQFFRQWLKEQI 234 PT+ILYGSDDNL E D +SAFA R+ +KV+E GEHYFHT+EQL F +W E + Sbjct: 181 KVPTAILYGSDDNLSESDEISAFAERYQSTVKVLEHGEHYFHTEEQLTVFDRWADENL 238 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.325 0.139 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 354,316,635 Number of extensions: 14079423 Number of successful extensions: 43773 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 43771 Number of HSP's successfully gapped: 12 Length of query: 235 Length of database: 757,237,578 Length adjustment: 122 Effective length of query: 113 Effective length of database: 425,995,500 Effective search space: 48137491500 Effective search space used: 48137491500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 124 (52.4 bits)