BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0025.p (185 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00062043.1| COG1859: RNA:NAD 2'-phosphotransferase [Clost... 126 2e-28 sp|Q8U4K2|KPTA_PYRFU Probable RNA 2'-phosphotransferase 121 9e-27 ref|NP_561060.1| conserved hypothetical protein [Clostridium per... 117 2e-25 ref|NP_142162.1| hypothetical protein PH0160 [Pyrococcus horikos... 116 2e-25 ref|ZP_00178361.1| hypothetical protein Cwat162401 [Crocosphaera... 116 3e-25 >ref|ZP_00062043.1| COG1859: RNA:NAD 2'-phosphotransferase [Clostridium thermocellum ATCC 27405] Length = 182 Score = 126 bits (317), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 70/172 (40%), Positives = 101/172 (58%), Gaps = 3/172 (1%) Query: 10 LSKFMTKLLRHTPAEYGLLLDPEDGSCLLEDLERVIHTAPKWAGITEEDIRQVVRNSEKQ 69 LSK + LRH P EYGL LD E G + L +H KW ++E D+ ++ S+K+ Sbjct: 9 LSKEVAYALRHAPWEYGLELDAE-GWVDINQLLSSLHECEKWKKVSEHDLHVMIEKSDKK 67 Query: 70 RFAIEGDRIKARYGHS-HARLTYEPGIPPKVLYHGTHQAALPAILKEGLLPMGRQYIHLS 128 R+ I +I+A YGHS R+ E PP+VLYHGT + + +I ++GL P GRQY+HLS Sbjct: 68 RYEISNGKIRALYGHSIPQRIIKEQKCPPEVLYHGTARRFVKSIKEKGLQPQGRQYVHLS 127 Query: 129 EGLHFASLAGSRRG-KLILLTVDTVKAVHLGVTFYYAGNEVWLSEPIPPACI 179 + A G RR K +LL V+ ++A G+ FY ++VWL++ IP I Sbjct: 128 ADVETALQVGKRRDIKPVLLIVNALEAWSEGIKFYLGNDKVWLADAIPSKYI 179 >sp|Q8U4K2|KPTA_PYRFU Probable RNA 2'-phosphotransferase Length = 183 Score = 121 bits (303), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 74/182 (40%), Positives = 100/182 (54%), Gaps = 5/182 (2%) Query: 1 MLNHAAEVSLSKFMTKLLRHTPAEYGLLLDPEDGSCLLEDLERVIHTAPKWAGITEEDIR 60 M+ +SK M +LRH+P E+GL D E+G L DL R I T W +TEE I+ Sbjct: 1 MVGGDRRFKVSKLMAYILRHSPWEFGLEPD-EEGFVELADLIRAIKTVYPW--VTEEFIK 57 Query: 61 QVVRNSEKQRFAIEGDRIKARYGHSH-ARLTYEPGIPPKVLYHGTHQAALPAILKEGLLP 119 ++V K R+ I G +I+ARYGHS+ L +E KVLYHGT + L ILKEG+ P Sbjct: 58 EIVEKDSKGRYEIRGSKIRARYGHSYPVVLKHEEDTESKVLYHGTIRENLKGILKEGIKP 117 Query: 120 MGRQYIHLSEGLHFASLAGSRRGK-LILLTVDTVKAVHLGVTFYYAGNEVWLSEPIPPAC 178 M RQY+HLS A G R GK +++L +D G AG V + + +P C Sbjct: 118 MKRQYVHLSLSYEDAYYTGRRHGKDVVVLLIDAECLRRKGYKILKAGKRVRIVKHVPVEC 177 Query: 179 IA 180 I+ Sbjct: 178 IS 179 >ref|NP_561060.1| conserved hypothetical protein [Clostridium perfringens] sp|Q8XP22|KPTA_CLOPE Probable RNA 2'-phosphotransferase dbj|BAB79850.1| conserved hypothetical protein [Clostridium perfringens str. 13] Length = 183 Score = 117 bits (292), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 68/182 (37%), Positives = 105/182 (57%), Gaps = 8/182 (4%) Query: 1 MLNHAAEVSLSKFMTKLLRHTPAEYGLLLDPEDGSCLLEDLERVIHTAPKWAGITEEDIR 60 M N+ +++S K+++ +LRH P E GL LD E G + DL ++ + + G + +D+ Sbjct: 1 MKNNDSKIS--KYISLILRHKPEEIGLKLD-EHGYLGVLDLIEGLNKS--YKGFSMDDLE 55 Query: 61 QVVRNSEKQRFAIEGDR--IKARYGHS-HARLTYEPGIPPKVLYHGTHQAALPAILKEGL 117 ++VR K R++ D+ I+A GHS L E PPKVLYHGT + + +ILK GL Sbjct: 56 RIVREDSKGRYSFNEDKSKIRANQGHSIKVDLGLEAIKPPKVLYHGTGRKYIESILKNGL 115 Query: 118 LPMGRQYIHLSEGLHFASLAGSRRGKLILLTVDTVKAVHLGVTFYYAGNEVWLSEPIPPA 177 + RQY+HLS+ + AS+ G R G L++L VD+ G+ FY + N VWL + + Sbjct: 116 IKKERQYVHLSKDIETASIVGKRHGDLVILEVDSESMFKDGIKFYLSKNNVWLCDYVKVE 175 Query: 178 CI 179 I Sbjct: 176 YI 177 >ref|NP_142162.1| hypothetical protein PH0160 [Pyrococcus horikoshii] sp|O57899|KPTA_PYRHO Probable RNA 2'-phosphotransferase pir||F71237 hypothetical protein PH0160 - Pyrococcus horikoshii dbj|BAA29229.1| 177aa long hypothetical protein [Pyrococcus horikoshii] Length = 177 Score = 116 bits (291), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 68/174 (39%), Positives = 101/174 (58%), Gaps = 5/174 (2%) Query: 9 SLSKFMTKLLRHTPAEYGLLLDPEDGSCLLEDLERVIHTAPKWAGITEEDIRQVVRNSEK 68 +SK M +LRH+P E+GL D E+G +E+L + W +TEE IR++V EK Sbjct: 4 KVSKLMAYILRHSPWEFGLEPD-EEGFVSIEELVNAVRKVYPW--VTEEYIREIVERDEK 60 Query: 69 QRFAIEGDRIKARYGHSH-ARLTYEPGIPPKVLYHGTHQAALPAILKEGLLPMGRQYIHL 127 R+ I G++I+ARYGHS+ L +E KVLYHGT + L I++EG+ PM RQY+HL Sbjct: 61 GRYEIRGNKIRARYGHSYPVILRHEEDKESKVLYHGTVRRNLKGIMREGIKPMKRQYVHL 120 Query: 128 SEGLHFASLAGSRRGK-LILLTVDTVKAVHLGVTFYYAGNEVWLSEPIPPACIA 180 S A G R G+ +++L +D + G AG +V + + +P CI+ Sbjct: 121 SINYEDAYNTGRRHGEDVVVLIIDAECLRNKGYKILKAGKKVRIVKHVPVDCIS 174 >ref|ZP_00178361.1| hypothetical protein Cwat162401 [Crocosphaera watsonii WH 8501] Length = 181 Score = 116 bits (290), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 67/178 (37%), Positives = 98/178 (55%), Gaps = 6/178 (3%) Query: 8 VSLSKFMTKLLRHTPAEYGLLLDPEDGSCLLEDLERVIHTAPKWAGITEEDIRQVVRNSE 67 + +S+F++ LRH P + GL L+P G E L + +T D+ VV++++ Sbjct: 7 IKISRFLSYHLRHAPEKLGLELEP-GGWISTEKLLKACQNTK--FTLTFSDLETVVKDND 63 Query: 68 KQRFAIE--GDRIKARYGHS-HARLTYEPGIPPKVLYHGTHQAALPAILKEGLLPMGRQY 124 KQRF+ +I+A GHS + L EP IPP +LYHGTHQ + ILKEGL M R + Sbjct: 64 KQRFSFNEAKTKIRANQGHSVNIDLQLEPLIPPVILYHGTHQKVVKTILKEGLKKMARHH 123 Query: 125 IHLSEGLHFASLAGSRRGKLILLTVDTVKAVHLGVTFYYAGNEVWLSEPIPPACIAMR 182 +HLSE A GSRRGK ++ + + G FY + N VWL + I P + ++ Sbjct: 124 VHLSERKETALKVGSRRGKPVIFVIKAQQMSEDGCIFYCSDNGVWLVDYIDPKYLTIK 181 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 279,194,663 Number of extensions: 10824619 Number of successful extensions: 32014 Number of sequences better than 1.0e-05: 53 Number of HSP's gapped: 31942 Number of HSP's successfully gapped: 53 Length of query: 185 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 67 Effective length of database: 436,855,896 Effective search space: 29269345032 Effective search space used: 29269345032 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 122 (51.6 bits)