BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0018.p (340 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_832576.1| Manganese-dependent inorganic pyrophosphatase [... 506 e-142 ref|NP_845172.1| inorganic pyrophosphatase, manganese-dependent ... 503 e-141 ref|NP_979158.1| inorganic pyrophosphatase, manganese-dependent ... 503 e-141 ref|NP_656707.1| DHHA2, DHHA2 domain [Bacillus anthracis A2012] 500 e-140 ref|NP_391935.1| similar to hypothetical proteins [Bacillus subt... 469 e-131 >ref|NP_832576.1| Manganese-dependent inorganic pyrophosphatase [Bacillus cereus ATCC 14579] gb|AAP09777.1| Manganese-dependent inorganic pyrophosphatase [Bacillus cereus ATCC 14579] Length = 309 Score = 506 bits (1303), Expect = e-142, Method: Compositional matrix adjust. Identities = 244/305 (80%), Positives = 270/305 (88%) Query: 31 MEKTLIFGHKNPDTDTICSAIAYAALKKELGWDAEPVRLGEISGETQYALDHFGVKAPRL 90 MEK L+FGHKNPDTD ICSAIAYA LKKELG +AEPVRLGEISGETQ+ALD+F V+ PR Sbjct: 1 MEKVLVFGHKNPDTDAICSAIAYAELKKELGMNAEPVRLGEISGETQFALDYFKVEGPRF 60 Query: 91 VENVASEAKQVILVDHNERQQSANDIDQVRVVEVIDHHRIANFETAHPLYYRAEPVGCTA 150 VE VASE VILVDHNERQQSANDI+ VRV+EVIDHHRIANFET+ P+YYR EPVGCTA Sbjct: 61 VETVASEVDNVILVDHNERQQSANDIESVRVLEVIDHHRIANFETSDPIYYRCEPVGCTA 120 Query: 151 TILNKLYKENGVAIPKDIAGLMLSAIISDSLLFKSPTCTEQDVAAARELAGIAGVDADSY 210 TILNK+YKENGV I K++AGLMLSAIISDSLLFKSPTCTEQDVAAARELA IAGVDAD+Y Sbjct: 121 TILNKMYKENGVTIRKEVAGLMLSAIISDSLLFKSPTCTEQDVAAARELAQIAGVDADNY 180 Query: 211 GLAMLKAGADLSDKTIAQLISLDAKEFKMGEYKVEIAQVNAVDVNDVLSKQAELETALTA 270 GL MLKAGADLS KT+ QLISLDAKEF+MG KVEIAQVNAVD NDVL QAELE ++A Sbjct: 181 GLEMLKAGADLSGKTMEQLISLDAKEFQMGNAKVEIAQVNAVDTNDVLVHQAELEKVISA 240 Query: 271 IIDDKGLDLFLFVVTDILNNDSVGLALGRVAGAVEQAYNVKLDDNKALLKGVVSRKSQIV 330 ++++KGLDLFLFVVTDIL NDSVGLA+G+ A VE+AYNV L++N A LKGVVSRK QIV Sbjct: 241 VVEEKGLDLFLFVVTDILTNDSVGLAIGKAANVVEKAYNVSLENNTATLKGVVSRKKQIV 300 Query: 331 PVLTE 335 PVLTE Sbjct: 301 PVLTE 305 >ref|NP_845172.1| inorganic pyrophosphatase, manganese-dependent [Bacillus anthracis str. Ames] gb|AAP26658.1| inorganic pyrophosphatase, manganese-dependent [Bacillus anthracis str. Ames] Length = 309 Score = 503 bits (1296), Expect = e-141, Method: Compositional matrix adjust. Identities = 243/305 (79%), Positives = 269/305 (88%) Query: 31 MEKTLIFGHKNPDTDTICSAIAYAALKKELGWDAEPVRLGEISGETQYALDHFGVKAPRL 90 MEK L+FGHKNPDTD ICSAIAYA LKKELG +AEPVRLGEISGETQ+ALD+F V+ PR Sbjct: 1 MEKVLVFGHKNPDTDAICSAIAYAELKKELGMNAEPVRLGEISGETQFALDYFKVEGPRF 60 Query: 91 VENVASEAKQVILVDHNERQQSANDIDQVRVVEVIDHHRIANFETAHPLYYRAEPVGCTA 150 VE VA+E VILVDHNERQQSANDI+ VRV+EVIDHHRIANFET+ P+YYR EPVGCTA Sbjct: 61 VETVANEVDNVILVDHNERQQSANDIESVRVLEVIDHHRIANFETSDPIYYRCEPVGCTA 120 Query: 151 TILNKLYKENGVAIPKDIAGLMLSAIISDSLLFKSPTCTEQDVAAARELAGIAGVDADSY 210 TILNK+YKENGV I K++AGLMLSAIISDSLLFKSPTCTEQDVAAARELA IAGVDAD Y Sbjct: 121 TILNKMYKENGVTIRKEVAGLMLSAIISDSLLFKSPTCTEQDVAAARELAEIAGVDADKY 180 Query: 211 GLAMLKAGADLSDKTIAQLISLDAKEFKMGEYKVEIAQVNAVDVNDVLSKQAELETALTA 270 GL MLKAGADLS KT+ QLISLDAKEF+MG KVEIAQVNAVD NDVL QAELE ++A Sbjct: 181 GLEMLKAGADLSGKTMEQLISLDAKEFQMGNAKVEIAQVNAVDTNDVLVHQAELEKVISA 240 Query: 271 IIDDKGLDLFLFVVTDILNNDSVGLALGRVAGAVEQAYNVKLDDNKALLKGVVSRKSQIV 330 ++++KGLDLFLFVVTDIL NDSVGLA+G+ A VE+AYNV L++N A LKGVVSRK QIV Sbjct: 241 VVEEKGLDLFLFVVTDILTNDSVGLAIGKAANIVEKAYNVSLENNTATLKGVVSRKKQIV 300 Query: 331 PVLTE 335 PVLTE Sbjct: 301 PVLTE 305 >ref|NP_979158.1| inorganic pyrophosphatase, manganese-dependent [Bacillus cereus ATCC 10987] gb|AAS41766.1| inorganic pyrophosphatase, manganese-dependent [Bacillus cereus ATCC 10987] Length = 309 Score = 503 bits (1295), Expect = e-141, Method: Compositional matrix adjust. Identities = 242/305 (79%), Positives = 269/305 (88%) Query: 31 MEKTLIFGHKNPDTDTICSAIAYAALKKELGWDAEPVRLGEISGETQYALDHFGVKAPRL 90 MEK L+FGHKNPDTD ICSAIAYA LKKELG +AEPVRLGEISGETQ+ALD+F V+ PR Sbjct: 1 MEKVLVFGHKNPDTDAICSAIAYAELKKELGMNAEPVRLGEISGETQFALDYFKVEGPRF 60 Query: 91 VENVASEAKQVILVDHNERQQSANDIDQVRVVEVIDHHRIANFETAHPLYYRAEPVGCTA 150 VE VA+E VILVDHNERQQSANDI+ VRV+EVIDHHRIANFET+ P+YYR EPVGCTA Sbjct: 61 VETVANEVDNVILVDHNERQQSANDIESVRVLEVIDHHRIANFETSDPIYYRCEPVGCTA 120 Query: 151 TILNKLYKENGVAIPKDIAGLMLSAIISDSLLFKSPTCTEQDVAAARELAGIAGVDADSY 210 TILNK+YKENG+ I K++AGLMLSAIISDSLLFKSPTCTEQDVAAARELA IAGVDAD+Y Sbjct: 121 TILNKMYKENGITIRKEVAGLMLSAIISDSLLFKSPTCTEQDVAAARELAEIAGVDADNY 180 Query: 211 GLAMLKAGADLSDKTIAQLISLDAKEFKMGEYKVEIAQVNAVDVNDVLSKQAELETALTA 270 GL MLKAGADLS KT+ QLISLDAKEF+MG KVEIAQVNAVD NDVL QAELE ++A Sbjct: 181 GLEMLKAGADLSGKTMEQLISLDAKEFQMGNAKVEIAQVNAVDTNDVLVHQAELEKVISA 240 Query: 271 IIDDKGLDLFLFVVTDILNNDSVGLALGRVAGAVEQAYNVKLDDNKALLKGVVSRKSQIV 330 ++++KGLDLFLFVVTDIL NDSVGLA+G A VE+AYNV L++N A LKGVVSRK QIV Sbjct: 241 VVEEKGLDLFLFVVTDILTNDSVGLAIGNAANVVEKAYNVSLENNTATLKGVVSRKKQIV 300 Query: 331 PVLTE 335 PVLTE Sbjct: 301 PVLTE 305 >ref|NP_656707.1| DHHA2, DHHA2 domain [Bacillus anthracis A2012] Length = 309 Score = 500 bits (1287), Expect = e-140, Method: Compositional matrix adjust. Identities = 241/305 (79%), Positives = 267/305 (87%) Query: 31 MEKTLIFGHKNPDTDTICSAIAYAALKKELGWDAEPVRLGEISGETQYALDHFGVKAPRL 90 MEK L+FGHKNPDTD ICSAIAYA LKKELG +AEPVRLGEISGETQ+ALD+F V+ PR Sbjct: 1 MEKVLVFGHKNPDTDAICSAIAYAELKKELGMNAEPVRLGEISGETQFALDYFKVEGPRF 60 Query: 91 VENVASEAKQVILVDHNERQQSANDIDQVRVVEVIDHHRIANFETAHPLYYRAEPVGCTA 150 VE VA+E VILVDHNERQQSANDI+ VRV+EVIDHHRIANFET+ P+YYR EPVGCTA Sbjct: 61 VETVANEVDNVILVDHNERQQSANDIESVRVLEVIDHHRIANFETSDPIYYRCEPVGCTA 120 Query: 151 TILNKLYKENGVAIPKDIAGLMLSAIISDSLLFKSPTCTEQDVAAARELAGIAGVDADSY 210 TILNK+YKENGV I K++AGLMLSAIISDSLLFKSPTCT QDV AARELA IAGVDAD Y Sbjct: 121 TILNKMYKENGVTIRKEVAGLMLSAIISDSLLFKSPTCTXQDVXAARELAEIAGVDADKY 180 Query: 211 GLAMLKAGADLSDKTIAQLISLDAKEFKMGEYKVEIAQVNAVDVNDVLSKQAELETALTA 270 GL MLKAGADLS KT+ QLISLDAKEF+MG KVEIAQVNAVD NDVL QAELE ++A Sbjct: 181 GLEMLKAGADLSGKTMEQLISLDAKEFQMGNAKVEIAQVNAVDTNDVLVHQAELEKVISA 240 Query: 271 IIDDKGLDLFLFVVTDILNNDSVGLALGRVAGAVEQAYNVKLDDNKALLKGVVSRKSQIV 330 ++++KGLDLFLFVVTDIL NDSVGLA+G+ A VE+AYNV L++N A LKGVVSRK QIV Sbjct: 241 VVEEKGLDLFLFVVTDILTNDSVGLAIGKAANIVEKAYNVSLENNTATLKGVVSRKKQIV 300 Query: 331 PVLTE 335 PVLTE Sbjct: 301 PVLTE 305 >ref|NP_391935.1| similar to hypothetical proteins [Bacillus subtilis] sp|P37487|PPAC_BACSU Manganese-dependent inorganic pyrophosphatase (Pyrophosphate phospho-hydrolase) (PPase) pir||S65980 conserved hypothetical protein yybQ - Bacillus subtilis dbj|BAA05186.1| unknown [Bacillus subtilis] emb|CAB16092.1| inorganic pyrophosphatase [Bacillus subtilis subsp. subtilis str. 168] Length = 309 Score = 469 bits (1206), Expect = e-131, Method: Compositional matrix adjust. Identities = 224/309 (72%), Positives = 265/309 (85%) Query: 31 MEKTLIFGHKNPDTDTICSAIAYAALKKELGWDAEPVRLGEISGETQYALDHFGVKAPRL 90 MEK LIFGH+NPDTDTICSAIAYA LK +LG++AEPVRLG+++GETQYALD+F ++PRL Sbjct: 1 MEKILIFGHQNPDTDTICSAIAYADLKNKLGFNAEPVRLGQVNGETQYALDYFKQESPRL 60 Query: 91 VENVASEAKQVILVDHNERQQSANDIDQVRVVEVIDHHRIANFETAHPLYYRAEPVGCTA 150 VE A+E VILVDHNERQQS DI++V+V+EVIDHHRIANFETA PLYYRAEPVGCTA Sbjct: 61 VETAANEVNGVILVDHNERQQSIKDIEEVQVLEVIDHHRIANFETAEPLYYRAEPVGCTA 120 Query: 151 TILNKLYKENGVAIPKDIAGLMLSAIISDSLLFKSPTCTEQDVAAARELAGIAGVDADSY 210 TILNK+YKEN V I K+IAGLMLSAIISDSLLFKSPTCT+QDVAAA+ELA IAGVDA+ Y Sbjct: 121 TILNKMYKENNVKIEKEIAGLMLSAIISDSLLFKSPTCTDQDVAAAKELAEIAGVDAEEY 180 Query: 211 GLAMLKAGADLSDKTIAQLISLDAKEFKMGEYKVEIAQVNAVDVNDVLSKQAELETALTA 270 GL MLKAGADLS KT+ +LISLDAKEF +G KVEIAQVN VD+ DV +QAELE ++ Sbjct: 181 GLNMLKAGADLSKKTVEELISLDAKEFTLGSKKVEIAQVNTVDIEDVKKRQAELEAVISK 240 Query: 271 IIDDKGLDLFLFVVTDILNNDSVGLALGRVAGAVEQAYNVKLDDNKALLKGVVSRKSQIV 330 ++ +K LDLFL V+TDIL NDS+ LA+G A VE+A+NV L++N ALLKGVVSRK Q+V Sbjct: 241 VVAEKNLDLFLLVITDILENDSLALAIGNEAAKVEKAFNVTLENNTALLKGVVSRKKQVV 300 Query: 331 PVLTETIAK 339 PVLT+ +A+ Sbjct: 301 PVLTDAMAE 309 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 450,939,778 Number of extensions: 17374960 Number of successful extensions: 50906 Number of sequences better than 1.0e-05: 81 Number of HSP's gapped: 50789 Number of HSP's successfully gapped: 94 Length of query: 340 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 214 Effective length of database: 415,135,104 Effective search space: 88838912256 Effective search space used: 88838912256 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)