BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0010.p (373 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_149193.1| Permease MDR-related [Clostridium acetobutylicu... 184 3e-45 ref|NP_243458.1| BH2592~unknown conserved protein [Bacillus halo... 174 3e-42 gb|EAJ27433.1| unknown [environmental sequence] 172 2e-41 ref|NP_844275.1| major facilitator family transporter [Bacillus ... 171 3e-41 ref|NP_459377.1| putative permease [Salmonella typhimurium LT2] ... 170 4e-41 >ref|NP_149193.1| Permease MDR-related [Clostridium acetobutylicum] gb|AAK76775.1| Permease MDR-related [Clostridium acetobutylicum] Length = 399 Score = 184 bits (467), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 109/350 (31%), Positives = 181/350 (51%), Gaps = 7/350 (2%) Query: 5 WKRNLIVLWVGVFFCSTAYXXXXXXXXXXXXDQLGVKNH--LEIWSGVSFGITFLASALI 62 WK+NLIV W G+F S LGV + +E +SG++FGITF+ SA+ Sbjct: 4 WKKNLIVCWFGMFITSIG-MSQIAPVLPLYIRHLGVNSSSLVEEFSGIAFGITFIVSAIF 62 Query: 63 SPYWGSLADKYGRKPMLIRSGFSLAALYLINFFVHDPYVFIIVRIFQGLLAGFVPAAIAM 122 SP WG ADK+GRKPML+R+ F + + FV + YV I++R+ QG++ G+ A + Sbjct: 63 SPIWGQAADKFGRKPMLLRASFGMGIIIFSMGFVSNVYVLIVLRLLQGVITGYSTACTTL 122 Query: 123 VATNTPEEKTGYALSIMSTAGATGSIIGPLIGGVVSFYSSNRSAFLFSAVIVLVSALIAT 182 +AT T +E G+AL +STAG GS++GP IGG V ++ F + ++++ + Sbjct: 123 IATQTDKEHAGWALGTLSTAGIAGSLLGPTIGGFVEEAFGTQNVFFITGALLIIVFIATM 182 Query: 183 FFAKEENYDRSAPRSHVRDDIREAAANPAFMTLLMLAGLATFSVMILEPLLPIYLLDMGI 242 F KE+ + ++ +T+L+ + T ++ +EP++ Y+ + Sbjct: 183 LFVKEDFVRENKKVDSMKKVWNSVPEKSLTITILVTFFILTLALYSIEPIMTEYVSKL-- 240 Query: 243 SRNSASLS--SGIVFSAVGIATVIMAPQWGRLGSRKXXXXXXXXXXXXXXXXXXXQYFVS 300 SRN++ ++ +G+VFSA G+A ++ AP+ G++ + Q FV Sbjct: 241 SRNTSHVAFIAGLVFSASGLANIVAAPRLGKISDKIGPHKIMLGALIVAGIVFIPQAFVK 300 Query: 301 GYVEFAILRFAYGLFYAGVLPSVNAMIVQVIDPGFRGRAFGLNQAATQLA 350 + RF GL G+ PSVN ++ ++ GR FGL+ +A L Sbjct: 301 NPWQLMFFRFLLGLASGGLTPSVNILVKKITPTAITGRIFGLSMSAGYLG 350 >ref|NP_243458.1| BH2592~unknown conserved protein [Bacillus halodurans] pir||H83973 hypothetical protein BH2592 [imported] - Bacillus halodurans (strain C-125) dbj|BAB06311.1| BH2592~unknown conserved protein [Bacillus halodurans] Length = 409 Score = 174 bits (442), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 107/351 (30%), Positives = 180/351 (51%), Gaps = 5/351 (1%) Query: 5 WKRNLIVLWVGVFFCSTAYXXXXXXXXXXXXDQLGVKNHLEI--WSGVSFGITFLASALI 62 WKRNL +L V F +A +LGV + E+ W+G+ F FL + L Sbjct: 4 WKRNLYILVVCQFLVVSA-MTMIIPFLPLYLQELGVTDPSEVSLWAGIIFAANFLTAFLF 62 Query: 63 SPYWGSLADKYGRKPMLIRSGFSLAALYLINFFVHDPYVFIIVRIFQGLLAGFVPAAIAM 122 SP W LAD+YGRK M++RSGF +A + + F P + +R+ G+++GF+PA+I + Sbjct: 63 SPLWSRLADRYGRKMMILRSGFGMALIIGLTGFAQGPLSLLCLRLLNGVISGFIPASIGL 122 Query: 123 VATNTPEEKTGYALSIMSTAGATGSIIGPLIGGVVSFYSSNRSAFLFSAVIVLVSALIAT 182 VATNTP+EK GYAL + +G I GPLIGG+++ R F + + + ++AL+ Sbjct: 123 VATNTPKEKAGYALGFLQAGAVSGGICGPLIGGIMAQAFGYRMIFFLTGLCIFIAALVVL 182 Query: 183 FFAKEE-NYDRSAPRSHVRDDIREAAANPAFMTLLMLAGLATFSVMILEPLLPIYLLDMG 241 F E+ + +++ + ++ A +L ++ L F+VM + PL+ +++ ++ Sbjct: 183 LFVNEQFTPNPKEEKTNTWGEFKKITAIRPIFSLYVVVFLIQFAVMGVNPLISLFVEEL- 241 Query: 242 ISRNSASLSSGIVFSAVGIATVIMAPQWGRLGSRKXXXXXXXXXXXXXXXXXXXQYFVSG 301 +S + +L +G+ SA G A ++ + Q G+L + Q FV Sbjct: 242 VSGVNVALFAGLAISATGFANMLSSMQLGKLSDKAGAHHVLFFCLLGTVIFMIPQAFVQQ 301 Query: 302 YVEFAILRFAYGLFYAGVLPSVNAMIVQVIDPGFRGRAFGLNQAATQLATM 352 + +LRF GL G+LP+ NA++ G R +G + +A L M Sbjct: 302 LWQLIVLRFFVGLSLGGLLPAANALVRMHAPKGMESRTYGFSTSALYLGNM 352 >gb|EAJ27433.1| unknown [environmental sequence] Length = 415 Score = 172 bits (435), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 115/348 (33%), Positives = 182/348 (52%), Gaps = 10/348 (2%) Query: 4 SWKRNLIVLWVGVFFCSTAYXXXXXXXXXXXXDQLGVKNH--LEIWSGVSFGITFLASAL 61 +WKRNL V W+G F A+ +QLGV H L +WSG+ F ITFL SA+ Sbjct: 9 NWKRNLTVTWLGCFLTGAAFSLVMPFLPLYV-EQLGVTGHSALNMWSGLVFSITFLFSAV 67 Query: 62 ISPYWGSLADKYGRKPMLIRSGFSLAALYLINFFVHDPYVFIIVRIFQGLLAGFVPAAIA 121 SP+WG LAD+ GRK ML+RS +A + ++ + + F+I+R GLL GF+P A A Sbjct: 68 ASPFWGGLADRKGRKIMLLRSALGMAIVMMLMGLAQNIWQFLILRALLGLLGGFIPNANA 127 Query: 122 MVATNTPEEKTGYALSIMSTAGATGSIIGPLIGGVVSFYSSNRSAFLFSAVIVLVSALIA 181 ++AT P +K+G+AL +ST +G+++GPL GG ++ + R+ F +A ++LV ++ Sbjct: 128 LIATQIPRQKSGWALGTLSTGAVSGALLGPLAGGFLADHWGLRTVFFLTASVLLVCFILT 187 Query: 182 TFFAKEENYDRSAPRSHVRDDIREAAANPAFMTLL----MLAGLATFSVMILEPLLPIYL 237 FF +E+ + D+ + +P + L M+ +AT S+ P+L +Y+ Sbjct: 188 LFFIREQFVPVAKKEMLNARDVFSSLKSPKLVLSLFVTTMIIQVATGSI---APILTLYV 244 Query: 238 LDMGISRNSASLSSGIVFSAVGIATVIMAPQWGRLGSRKXXXXXXXXXXXXXXXXXXXQY 297 D+ + + SG++ S G+A +I AP+ G+LG R Sbjct: 245 RDLAGDVGNIAFISGMIASVPGVAALISAPRLGKLGDRIGPEKILIVALMVSVLLLIPMS 304 Query: 298 FVSGYVEFAILRFAYGLFYAGVLPSVNAMIVQVIDPGFRGRAFGLNQA 345 FV ++ AILRF G +LP+V ++V GR F NQ+ Sbjct: 305 FVQTPLQLAILRFLLGAADGALLPAVQTLLVYNSTSQVAGRIFSYNQS 352 >ref|NP_844275.1| major facilitator family transporter [Bacillus anthracis str. Ames] ref|NP_978259.1| major facilitator family transporter [Bacillus cereus ATCC 10987] gb|AAP25761.1| major facilitator family transporter [Bacillus anthracis str. Ames] gb|AAS40867.1| major facilitator family transporter [Bacillus cereus ATCC 10987] Length = 404 Score = 171 bits (433), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 116/353 (32%), Positives = 183/353 (51%), Gaps = 6/353 (1%) Query: 4 SWKRNLIVLWVGVFFCSTAYXXXXXXXXXXXXDQLGVKNHLEI--WSGVSFGITFLASAL 61 SWKRNL++ W+G F + A ++LGV I WSG++FG+TFL A+ Sbjct: 3 SWKRNLMICWLGCF-TTAAGMSLVIPFLSFYIEELGVTGTSSIAQWSGLAFGVTFLMGAI 61 Query: 62 ISPYWGSLADKYGRKPMLIRSGFSLAALYLINFFVHDPYVFIIVRIFQGLLAGFVPAAIA 121 +SP WG L D +GRK MLIR+ +A + + FV D Y + +R G ++GF+ A+ Sbjct: 62 VSPIWGKLGDIHGRKLMLIRASLGMAIIMTLMGFVTDVYQLVALRFLMGAVSGFLSTAMT 121 Query: 122 MVATNTPEEKTGYALSIMSTAGATGSIIGPLIGGVVSFYSSNRSAFLFSAVIVLVSALIA 181 +A TP+E +G+A+S +ST G +GS++GPL+GG +S R FL + + +S LI Sbjct: 122 FIAAETPKEHSGWAISTISTGGVSGSLLGPLLGGYLSELIGMRHVFLVTGAFLFLSFLIV 181 Query: 182 TFFAKEENYDRSAPRSHVRDDIREAAANPAFMTLLMLAGLATFSVMILEPLLPIYLLDMG 241 FF EEN+ A + + A ++L + + + M ++P++ +Y+ ++ Sbjct: 182 FFFLHEENHSAQAKKVQTKKVWTMVPAKHLIISLFVTTFIIQLANMSVQPIVTLYVKNL- 240 Query: 242 ISRNSASLSS--GIVFSAVGIATVIMAPQWGRLGSRKXXXXXXXXXXXXXXXXXXXQYFV 299 + +A + + G V SA G+A ++ AP+ GRL Q FV Sbjct: 241 VGPQTAHIETIAGAVMSATGLAVILAAPRLGRLSDHIGPQKTLVVALFAAGIIFIPQAFV 300 Query: 300 SGYVEFAILRFAYGLFYAGVLPSVNAMIVQVIDPGFRGRAFGLNQAATQLATM 352 + + ILRF G+ AG+LPSV ++ Q GR FG NQ+ L M Sbjct: 301 TSAWQLLILRFLLGIAQAGLLPSVQTLLKQHTPTHVTGRIFGYNQSFQFLGNM 353 Score = 57.4 bits (137), Expect = 5e-07, Method: Compositional matrix adjust. Identities = 36/152 (23%), Positives = 73/152 (48%), Gaps = 1/152 (0%) Query: 43 HLEIWSGVSFGITFLASALISPYWGSLADKYGRKPMLIRSGFSLAALYLINFFVHDPYVF 102 H+E +G T LA L +P G L+D G + L+ + F+ +++ FV + Sbjct: 247 HIETIAGAVMSATGLAVILAAPRLGRLSDHIGPQKTLVVALFAAGIIFIPQAFVTSAWQL 306 Query: 103 IIVRIFQGL-LAGFVPAAIAMVATNTPEEKTGYALSIMSTAGATGSIIGPLIGGVVSFYS 161 +I+R G+ AG +P+ ++ +TP TG + G++IGP++GG ++ ++ Sbjct: 307 LILRFLLGIAQAGLLPSVQTLLKQHTPTHVTGRIFGYNQSFQFLGNMIGPILGGQIAAHA 366 Query: 162 SNRSAFLFSAVIVLVSALIATFFAKEENYDRS 193 + F ++ ++ ++ + F K E Sbjct: 367 GFQYVFFSTSSLLFIACIWVYFHNKNEEVSEK 398 >ref|NP_459377.1| putative permease [Salmonella typhimurium LT2] gb|AAL19336.1| putative permease [Salmonella typhimurium LT2] Length = 403 Score = 170 bits (431), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 116/348 (33%), Positives = 183/348 (52%), Gaps = 9/348 (2%) Query: 4 SWKRNLIVLWVGVFFCSTAYXXXXXXXXXXXXDQLGVKNH--LEIWSGVSFGITFLASAL 61 SWK NLI +W G FF A QLGV +H L +WSG++F ITFL SA+ Sbjct: 3 SWKVNLISVWFGCFFTGLAISQILPFLPLYI-SQLGVSSHEALSMWSGLTFSITFLISAI 61 Query: 62 ISPYWGSLADKYGRKPMLIRSGFSLAALYLINFFVHDPYVFIIVRIFQGLLAGFVPAAIA 121 +SP WGSLAD+ GRK ML+R+ +A L+ F + ++R GL +G++P A+A Sbjct: 62 VSPMWGSLADRKGRKLMLLRASLGMAIAILLQAFATHVWQLFLLRGIMGLTSGYIPNAMA 121 Query: 122 MVATNTPEEKTGYALSIMSTAGATGSIIGPLIGGVVSFYSSNRSAFLFSAVIVLVSALIA 181 +VA+ P E++G+ALS +STA +G I GPL+GG V+ + R+ FL +A++++VS L+ Sbjct: 122 LVASQVPRERSGWALSTLSTAQISGVIGGPLMGGFVADHIGLRAVFLITAMLLVVSFLVT 181 Query: 182 TFFAKEENYDRSAPRSHVRDDIREAAANPAFMTLLMLAGLATFSVMI----LEPLLPIYL 237 F KE R + R + A+ + L++ T + + + P+L +++ Sbjct: 182 LFLIKEG--VRPVIKKSERLSGKAVFASLPYPALVISLFFTTMVIQLCNGSISPILALFI 239 Query: 238 LDMGISRNSASLSSGIVFSAVGIATVIMAPQWGRLGSRKXXXXXXXXXXXXXXXXXXXQY 297 M N+ + SG++ S GI+ +I AP+ G+LG R Sbjct: 240 KSMMPDSNNIAFLSGLIASVPGISALISAPRLGKLGDRIGTERILMATLICAVLLFFAMS 299 Query: 298 FVSGYVEFAILRFAYGLFYAGVLPSVNAMIVQVIDPGFRGRAFGLNQA 345 +V+ + +LRF G +LP+V ++V+ GR FG NQ+ Sbjct: 300 WVTTPFQLGLLRFLLGFADGAMLPAVQTLLVKYSSDQITGRIFGYNQS 347 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.327 0.140 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 445,040,716 Number of extensions: 15358849 Number of successful extensions: 69188 Number of sequences better than 1.0e-05: 577 Number of HSP's gapped: 70130 Number of HSP's successfully gapped: 611 Length of query: 373 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 246 Effective length of database: 412,420,005 Effective search space: 101455321230 Effective search space used: 101455321230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)