BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0008.p (443 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_561506.1| conserved hypothetical protein [Clostridium per... 424 e-117 ref|NP_816111.1| voltage-gated chloride channel family protein [... 385 e-105 gb|EAH89850.1| unknown [environmental sequence] 338 1e-91 ref|NP_902099.1| probable chloride channel protein EriC [Chromob... 338 2e-91 ref|NP_241529.1| BH0663~unknown conserved protein in others [Bac... 337 4e-91 >ref|NP_561506.1| conserved hypothetical protein [Clostridium perfringens] dbj|BAB80296.1| conserved hypothetical protein [Clostridium perfringens str. 13] Length = 437 Score = 424 bits (1091), Expect = e-117, Method: Compositional matrix adjust. Identities = 224/437 (51%), Positives = 301/437 (68%), Gaps = 1/437 (0%) Query: 1 MRAIHTYWLKLMERSHFLALWGTFIKWIVLGGCVGILSGSASAIFLKSLDAVTEVRLNHA 60 M+ +LK + R++ L TF+KW+++G +GI G AIFLKSL+ T+ R+NH Sbjct: 1 MKQKSKKYLKYISRNNTFLLGKTFLKWLLIGSLIGIFIGGVIAIFLKSLEWATDYRVNHG 60 Query: 61 WXXXXXXXXXXXVSFLYMRYGKNSAKGNNLILEQIQQGNETIPMRMAPLVLFGTLVTHLF 120 + VSFLY ++G+NS KGNNLI+E I +P+RM PLV FGT+VTH F Sbjct: 61 FVLFLLPIGGALVSFLYYKFGRNSVKGNNLIIENINNYCGDVPLRMVPLVFFGTVVTHFF 120 Query: 121 GGSAGREGTAVQMGGSLAEGFGRLIRINSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLE 180 GGSAGREGT VQ+G S+AE G+L+++N D +I+LM TPLAGT+FGLE Sbjct: 121 GGSAGREGTGVQIGASIAETIGKLLKLNKEDSKIMLMAGVSGGFSAIFGTPLAGTIFGLE 180 Query: 181 VVAIGLISHKALLPCFTASFIGDIVATRLWKVQHIHYHVELFPPLTAIILLKVILASVLF 240 V +G +S++AL+P F AS +G+ V ++ V H HY V ++ +I+LKVI++++ F Sbjct: 181 VSVLGKMSYEALIPSFFASIVGNEV-VKILGVHHSHYKVLGVADISVLIILKVIISAICF 239 Query: 241 GLASILFSELTHYLKKTFTRLVKNPMLKSALGGFIIIALVYIAGTRDYLGLGIPLIQDSF 300 GLAS LFSELTH LK F+ KN LKS +GG I+I LVY+ G+R YLGL +PL+ ++F Sbjct: 240 GLASRLFSELTHKLKGLFSNKFKNTSLKSFVGGCIVILLVYLVGSRKYLGLSLPLLSEAF 299 Query: 301 QTDVAPFAFLWKLIFTSFTLGTGFQGGEVTPLFAIGATLGNSLASFLHLHAPFLASLGFI 360 V+PF FL KLIFTS TLG G+QGGEVTPLF IG+TLGN+L+ L+L FLASLG + Sbjct: 300 NGHVSPFDFLGKLIFTSITLGAGYQGGEVTPLFVIGSTLGNTLSGILNLSPSFLASLGLV 359 Query: 361 AVFCGATNTPVACFLMGIELFGSGGAVYMFIACLISYLFSGHSGIYTSQQIGISKSEWVR 420 VF GATNTP+A F++GIE+FG GGA Y+F+AC ISY+FSGH+GIYTSQ+IG+SKS + Sbjct: 360 GVFAGATNTPIASFVLGIEMFGVGGAPYLFMACAISYIFSGHTGIYTSQKIGVSKSNLIN 419 Query: 421 IPEGTTLGTAKQLRTTK 437 +TL + + K Sbjct: 420 FSHDSTLASLSKREEVK 436 >ref|NP_816111.1| voltage-gated chloride channel family protein [Enterococcus faecalis V583] gb|AAO82181.1| voltage-gated chloride channel family protein [Enterococcus faecalis V583] Length = 421 Score = 385 bits (988), Expect = e-105, Method: Compositional matrix adjust. Identities = 194/398 (48%), Positives = 264/398 (66%), Gaps = 1/398 (0%) Query: 13 ERSHFLALWGTFIKWIVLGGCVGILSGSASAIFLKSLDAVTEVRLNHAWXXXXXXXXXXX 72 E L++ G KW+++ +G+ G+ SA FLKSL VT+VRL + W Sbjct: 4 ETKQLLSIGGYMAKWLLITAIIGLFMGALSAFFLKSLTYVTDVRLANPWLLFILPISGVV 63 Query: 73 VSFLYMRYGKNSAKGNNLILEQIQQGNETIPMRMAPLVLFGTLVTHLFGGSAGREGTAVQ 132 ++ Y R+GKN+++GNNL++EQ G E IP+R+ PL LFGT+ THLFGGS GREGTAVQ Sbjct: 64 FTYFYTRFGKNASRGNNLVIEQGNGGEEDIPLRLIPLTLFGTITTHLFGGSVGREGTAVQ 123 Query: 133 MGGSLAEGFGRLIRINSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLEVVAIGLISHKAL 192 MGG++A G++ ++++L+RQ++++ TPLAGT+FGLEV+A+G + +AL Sbjct: 124 MGGAIANAVGKVFKLSALERQVIIISGISAGFSSVFGTPLAGTVFGLEVLAVGKVRAEAL 183 Query: 193 LPCFTASFIGDIVATRLWKVQHIHYHVELFPPLTAIILLKVILASVLFGLASILFSELTH 252 P F A + V T + V H HY + P + + +K+ LAS+ FGLA +FS Sbjct: 184 FPSFFAGLFANFV-TESFGVTHTHYPMGKIPTWSVELFVKLFLASICFGLAGWVFSRSIV 242 Query: 253 YLKKTFTRLVKNPMLKSALGGFIIIALVYIAGTRDYLGLGIPLIQDSFQTDVAPFAFLWK 312 +LKKT+ N L++ LG I++ V + + YLGL +PL++D+F + PF F+ K Sbjct: 243 FLKKTYANWFANVYLRAFLGAAIVVLFVLVLNNQRYLGLSLPLLEDAFAGNAQPFDFVGK 302 Query: 313 LIFTSFTLGTGFQGGEVTPLFAIGATLGNSLASFLHLHAPFLASLGFIAVFCGATNTPVA 372 L FT +LG GFQGGEVTPLF IGATLG+SLA LHL PFLA LGFI VF GATNTP+A Sbjct: 303 LFFTVLSLGAGFQGGEVTPLFEIGATLGSSLAPLLHLSIPFLAGLGFIGVFSGATNTPIA 362 Query: 373 CFLMGIELFGSGGAVYMFIACLISYLFSGHSGIYTSQQ 410 CF+MGIELFGS AVY F+ CLIS++ SG+SGIY SQQ Sbjct: 363 CFIMGIELFGSEAAVYFFMVCLISFMCSGNSGIYASQQ 400 >gb|EAH89850.1| unknown [environmental sequence] Length = 413 Score = 338 bits (868), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 176/396 (44%), Positives = 245/396 (61%), Gaps = 1/396 (0%) Query: 26 KWIVLGGCVGILSGSASAIFLKSLDAVTEVRLNHAWXXXXXXXXXXXVSFLYMRYGKNSA 85 +W+ L +G L+G+ASA+FL +LD T R+ H W ++Y R+G++ A Sbjct: 18 RWLALSALLGTLAGTASALFLIALDWATGTRVAHPWLLWGLPAAGFATGWIYHRFGQSVA 77 Query: 86 KGNNLILEQIQQGNETIPMRMAPLVLFGTLVTHLFGGSAGREGTAVQMGGSLAEGFGRLI 145 +GNNL++++I +P RMAPLVL T+VTHLFGGSAGREGTAVQMGG+LA+ + Sbjct: 78 RGNNLLIDEIHDPKALVPKRMAPLVLVATVVTHLFGGSAGREGTAVQMGGALADRVTHVF 137 Query: 146 RINSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLEVVAIGLISHKALLPCFTASFIGDIV 205 R++ R++LLM TPLAG +FGLEV+AIG + + ALL C ++ + D+V Sbjct: 138 RLDREHRRVLLMGGIAAGFASVFGTPLAGAVFGLEVLAIGRVRYDALLTCVASAIVADVV 197 Query: 206 ATRLWKVQHIHYHVELFPPLTAIILLKVILASVLFGLASILFSELTHYLKKTFTRLVKNP 265 R W V H Y + P ++A L ++A + FG+ LF+ TH L F ++V+ Sbjct: 198 -CRAWGVHHTAYAIPFVPAVSATGLAVTVVAGIAFGVVGRLFAFATHALTAWFRKVVRYA 256 Query: 266 MLKSALGGFIIIALVYIAGTRDYLGLGIPLIQDSFQTDVAPFAFLWKLIFTSFTLGTGFQ 325 L+ LGG ++ A YLGLGIP IQ +F + + F K FT TL +GF+ Sbjct: 257 PLQPVLGGLLVAAAATALNVPQYLGLGIPTIQAAFHGPLPLYDFAGKFAFTVVTLASGFK 316 Query: 326 GGEVTPLFAIGATLGNSLASFLHLHAPFLASLGFIAVFCGATNTPVACFLMGIELFGSGG 385 GGEVTPLF IGATLGN+L L L P LA LGF+AVF GA NTP+A +M IELFG+ Sbjct: 317 GGEVTPLFYIGATLGNALGQVLALPVPVLAGLGFVAVFAGAANTPIASTIMAIELFGADI 376 Query: 386 AVYMFIACLISYLFSGHSGIYTSQQIGISKSEWVRI 421 VY +AC+++YLFSGH+GIY +Q++ + K V + Sbjct: 377 GVYAIVACVVAYLFSGHAGIYRAQRVAVGKGARVEV 412 >ref|NP_902099.1| probable chloride channel protein EriC [Chromobacterium violaceum ATCC 12472] gb|AAQ60101.1| probable chloride channel protein EriC [Chromobacterium violaceum ATCC 12472] Length = 438 Score = 338 bits (867), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 192/417 (46%), Positives = 252/417 (60%), Gaps = 3/417 (0%) Query: 26 KWIVLGGCVGILSGSASAIFLKSLDAVTEVRLNHAWXXXXXXXXXXXVSFLYMRYGKNSA 85 KW +L V LSG+ASA FL +L+ T RL H W V Y+R GK Sbjct: 18 KWSLLSALVAALSGTASAFFLFALEQATRTRLAHPWLIWLLPLGGFAVGMAYLRLGKEVE 77 Query: 86 KGNNLILEQIQQGNETIPMRMAPLVLFGTLVTHLFGGSAGREGTAVQMGGSLAEGFGRLI 145 GNNL++++I +T+P+RM PLVL GT+VTHLFGGSAGREGTAVQMGG+LA+ +++ Sbjct: 78 GGNNLLIDEIHDPQKTVPLRMVPLVLTGTVVTHLFGGSAGREGTAVQMGGALADQVHKVL 137 Query: 146 RINSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLEVVAIGLISHKALLPCFTASFIGDIV 205 + DR++LLM TPLAG +FGLE++AIG + + A+LPC A+ +GD Sbjct: 138 PLQPEDRRMLLMAGISVGFASVFGTPLAGAIFGLEMLAIGRLRYDAILPCLAAAILGD-Q 196 Query: 206 ATRLWKVQHIHYHVELFPPLTAIILLKVILASVLFGLASILFSELTHYLKKTFTRLVKNP 265 A+ LW V H Y + P L A L +LA FGL LF+E H+L R + Sbjct: 197 ASLLWGVHHTFYRIPFIPALDAWGLCASVLAGAAFGLTGKLFAESVHWLADCLRRRIAFA 256 Query: 266 MLKSALGGFIIIALVYIAGTRDYLGLGIPLIQDSFQTDVAPFAFLWKLIFTSFTLGTGFQ 325 L+ +GG I + R YLGLGIP + +FQ ++P+ + KL FT TLG+GF+ Sbjct: 257 PLRPLIGGGAIAVAASLMDARAYLGLGIPTLVSAFQQPLSPYDVIGKLGFTVATLGSGFK 316 Query: 326 GGEVTPLFAIGATLGNSLASFLHLHAPFLASLGFIAVFCGATNTPVACFLMGIELFGSGG 385 GGEVTPLF IGA LGN LA LHL P LA +GF+AVF GA NTP+A +M +ELFGS Sbjct: 317 GGEVTPLFYIGAALGNMLAPLLHLPFPLLAGMGFVAVFAGAANTPLASTVMALELFGSEI 376 Query: 386 AVYMFIACLISYLFSGHSGIYTSQQIGISKSEWVRIPEGTTLGTAKQLRTTKKTSAE 442 VY +AC+++YLFSGHS IY +Q+IG++K V PE L LR + E Sbjct: 377 GVYAGMACVVAYLFSGHSSIYRAQRIGVAKRRGV--PENIRLADWPALRQATRRKQE 431 >ref|NP_241529.1| BH0663~unknown conserved protein in others [Bacillus halodurans] pir||T44296 hypothetical protein BH0663 [imported] - Bacillus halodurans (strain C-125) dbj|BAA75315.1| similar to Bordetella paraperlussis transposase for insertion sequence element(27%-identity) [Bacillus halodurans] dbj|BAB04382.1| BH0663~unknown conserved protein in others [Bacillus halodurans] Length = 424 Score = 337 bits (863), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 172/419 (41%), Positives = 257/419 (61%), Gaps = 6/419 (1%) Query: 21 WGTFIKWIVLGGCVGILSGSASAIFLKSLDAVTEVRLNHAWXXXXXXXXXXXVSFLYMRY 80 W T + WI G +G + GSA+A+ L D + E R N W + +LYM+ Sbjct: 6 WLTLLTWIFFGIMIGAIVGSATALLLTVNDHLGETRENRPWFVLFLPLAGLALGYLYMKA 65 Query: 81 GKNSA----KGNNLILEQIQQGNETIPMRMAPLVLFGTLVTHLFGGSAGREGTAVQMGGS 136 G ++ KGNNL++E +Q G + +R+ PLV GT +T LFGGS GREG A+QMGGS Sbjct: 66 GTSAGNELYKGNNLVIESVQ-GKGKMLLRLGPLVYLGTFMTILFGGSTGREGAAIQMGGS 124 Query: 137 LAEGFGRLIRINSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLEVVAIGLISHKALLPCF 196 +AE +L ++ +D +ILLM TP+ +FG+E+ ++G + +AL+PC Sbjct: 125 VAEAVNKLFKVKLIDTRILLMGGISAGFGAAFGTPITAAIFGMEMASLGRLKFEALVPCL 184 Query: 197 TASFIGDIVATRLWKVQHIHYHVELFPPLTAIILLKVILASVLFGLASILFSELTHYLKK 256 ASF+G + W V+H + + P ++A+ KVIL +++F L S+L+ +L H + K Sbjct: 185 VASFVGHYTTEKFWHVEHEKFIIATVPEVSALTFSKVILLAIVFSLVSVLYCQLRHGIHK 244 Query: 257 TFTRL-VKNPMLKSALGGFIIIALVYIAGTRDYLGLGIPLIQDSFQTDVAPFAFLWKLIF 315 + +KN +++ +GG II+AL YI G+ DY G G+ +++DSF DV P+AFL KL+F Sbjct: 245 LSEKYTMKNHTVRAFVGGLIIVALTYIIGSYDYNGRGLDMLEDSFTQDVPPYAFLAKLVF 304 Query: 316 TSFTLGTGFQGGEVTPLFAIGATLGNSLASFLHLHAPFLASLGFIAVFCGATNTPVACFL 375 T+ T+G GF GGE PLF +GATLGN+L +F+ L FLA+LG I F G NTP+A FL Sbjct: 305 TAVTMGMGFVGGEAIPLFFVGATLGNTLHAFIDLPLSFLAALGMIVTFGGGANTPIAAFL 364 Query: 376 MGIELFGSGGAVYMFIACLISYLFSGHSGIYTSQQIGISKSEWVRIPEGTTLGTAKQLR 434 +G+E+F G + F+ACL SYLFSGH G++ SQ I KS + +G T+ ++++ Sbjct: 365 LGVEMFNGKGIEFFFVACLTSYLFSGHHGLWPSQTIYEPKSRLYGVRKGETIKRTEEMK 423 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.327 0.142 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 593,922,514 Number of extensions: 23275942 Number of successful extensions: 70653 Number of sequences better than 1.0e-05: 202 Number of HSP's gapped: 70408 Number of HSP's successfully gapped: 202 Length of query: 443 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 314 Effective length of database: 406,989,807 Effective search space: 127794799398 Effective search space used: 127794799398 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)