BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0034.p (297 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00180981.1| hypothetical protein Mther104601 [Moorella th... 102 1e-20 ref|NP_634483.1| methyltransferase [Methanosarcina mazei Goe1] >... 93 6e-18 ref|ZP_00078770.1| COG0500: SAM-dependent methyltransferases [Me... 84 3e-15 ref|NP_615800.1| conserved hypothetical protein [Methanosarcina ... 84 4e-15 ref|NP_618803.1| ribosomal RNA adenine dimethylase [Methanosarci... 73 6e-12 >ref|ZP_00180981.1| hypothetical protein Mther104601 [Moorella thermoacetica ATCC 39073] Length = 289 Score = 102 bits (254), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 53/187 (28%), Positives = 102/187 (54%), Gaps = 1/187 (0%) Query: 8 AAMWEKVWKDESDLKIDRMKKSGIDPTRSFDHKAKVFNEQSFNEKGRQRTKRIISWLEGQ 67 A WE+ W++E + ++ D + ++ +A F Q+ +E+G+QR I+ WL Sbjct: 9 ADFWEEAWREEHRNSLYGRRRPERDGSAYWNRRAGHFARQTGSEEGKQRVAGILHWLGHH 68 Query: 68 GVQFENASVLDIGAASGVFSVPFAEKGARVTAVETSPPLIELLEENVANFAEGQVKIVPE 127 G ++ VLDIG +G +++P A + RV A++ + ++ +L+E A V+ + Sbjct: 69 GGLEKDLEVLDIGCGTGNYALPLARRARRVVALDPAAEMLAILKEKAAAEGINNVEPIQM 128 Query: 128 PFEDIDVASMGWNQTFDLVFASMIPIIHNWESVEKILQCARKFCYISMPVLHTEHSLVKE 187 +E++D+ GW + FDLV A M P I + +++K++ +R C+++ V E S +E Sbjct: 129 AWEEVDLEKQGWREAFDLVLALMSPGIKDAATLQKMMAASRGACFLAGHV-RQEISGRRE 187 Query: 188 IWPLVTG 194 +W + G Sbjct: 188 LWQELIG 194 >ref|NP_634483.1| methyltransferase [Methanosarcina mazei Goe1] gb|AAM32155.1| methyltransferase [Methanosarcina mazei Goe1] Length = 287 Score = 93.2 bits (230), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 48/151 (31%), Positives = 83/151 (54%), Gaps = 1/151 (0%) Query: 54 RQRTKRIISWLEGQGVQFENASVLDIGAASGVFSVPFAEKGARVTAVETSPPLIELLEEN 113 ++RT I+ +LE G E + VLDIG G S+P ++ GA VTA++ S +++ L++ Sbjct: 53 KKRTDEILEFLEESGFDPEGSRVLDIGCGPGTLSLPLSKLGAEVTALDISSGMLKRLKDT 112 Query: 114 VANFAEGQVKIVPEPFEDIDVASMGWNQTFDLVFASMIPIIHNWESVEKILQCARKFCYI 173 V V ++ + D+ S+G+ FDLV ASM P I + E+ +K++ C++ FCY Sbjct: 113 VKE-ESLPVDVIETSWWTADIDSLGFRDRFDLVIASMTPAIKDIENFDKMMACSKNFCYY 171 Query: 174 SMPVLHTEHSLVKEIWPLVTGQSYQSKLTEM 204 S + E +EI + G++ + + M Sbjct: 172 SNFLRRGEDRAYREIRSSILGENSANNMNGM 202 >ref|ZP_00078770.1| COG0500: SAM-dependent methyltransferases [Methanosarcina barkeri] Length = 286 Score = 84.3 bits (207), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 44/149 (29%), Positives = 80/149 (53%), Gaps = 1/149 (0%) Query: 54 RQRTKRIISWLEGQGVQFENASVLDIGAASGVFSVPFAEKGARVTAVETSPPLIELLEEN 113 ++RT I+ LE G E + LDIG G ++P ++ GA VTA++ S +++ L+++ Sbjct: 52 KKRTDEILELLEEAGFNPEGSRALDIGCGPGTLTLPLSKLGAEVTALDISSGMLDRLKDS 111 Query: 114 VANFAEGQVKIVPEPFEDIDVASMGWNQTFDLVFASMIPIIHNWESVEKILQCARKFCYI 173 V + V IV + D+ +G+ FDLV ASM P + + ES +K++ C++ CY Sbjct: 112 VKKESL-PVDIVECSWWTADIDELGFRNEFDLVIASMTPGVKDIESFDKMMACSKNLCYY 170 Query: 174 SMPVLHTEHSLVKEIWPLVTGQSYQSKLT 202 S + E ++I + G+ ++ + Sbjct: 171 SNFLRREEDRAYRDIRSSILGEKSENNMN 199 >ref|NP_615800.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] gb|AAM04280.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] Length = 302 Score = 84.0 bits (206), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 54/220 (24%), Positives = 102/220 (46%), Gaps = 2/220 (0%) Query: 51 EKGRQRTKRIISWLEGQGVQFENASVLDIGAASGVFSVPFAEKGARVTAVETSPPLIELL 110 +K ++RT I+ +LE G E + VLDIG G S+P + GA VTA++ S +++ L Sbjct: 65 DKRKKRTDEILEFLEEAGFNPEGSRVLDIGCGPGTLSLPLSRLGAEVTALDISSGMLDRL 124 Query: 111 EENVANFAEGQVKIVPEPFEDIDVASMGWNQTFDLVFASMIPIIHNWESVEKILQCARKF 170 ++ V V ++ + D+ +G+ FDLV ASM P I + E+ ++ C++ Sbjct: 125 KDAVKK-ESLPVDVIECSWWTADIDELGFRNKFDLVIASMTPGIRDVENFNMMMACSKNL 183 Query: 171 CYISMPVLHTEHSLVKEIWPLVTGQSYQSKLTEMGXXXXXXXXXXXXXETLVTAEGKTRE 230 CY S + E ++I + G+ ++ + + + E Sbjct: 184 CYYSNFLRRVEDKAYRDIRSSILGERSENNMNGIIYPFMYLYLSGYSPSLRINHSEWKDE 243 Query: 231 IPGEMALQEVMTWLGHHRLPADERNMRIVANYLEQSYPSG 270 + A ++ + ++G R DE +I+ +Y + + P G Sbjct: 244 TNWQEAAEQAIGFIGRGRDFDDETKEKIM-DYYQNASPDG 282 >ref|NP_618803.1| ribosomal RNA adenine dimethylase [Methanosarcina acetivorans str. C2A] gb|AAM07283.1| ribosomal RNA adenine dimethylase [Methanosarcina acetivorans str. C2A] Length = 323 Score = 73.2 bits (178), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 60/193 (31%), Positives = 96/193 (49%), Gaps = 14/193 (7%) Query: 11 WEKVWKDESDLKIDRMKKSGIDPTRSFDHKAKVFNEQSFNEKGRQRTKRIISWLEGQGVQ 70 W +VWKD + + R +K A F E+S K QR ++ S L G G Sbjct: 51 WNEVWKDLHEQNLSRRRKGECASIWESRESALEFLERS--NKNPQRVAKVFSDL-GVG-- 105 Query: 71 FENASVLDIGAASGVFSVPFAEKGARVTAVETSPPLIELLEENVANFAEGQVKIVPEPFE 130 + VLD+GA G +VP A + A VTAVE + ++E+++E ++IV + +E Sbjct: 106 -PASRVLDVGAGPGTLAVPLASRCAHVTAVEPAAGMVEVMKEFAQKEGVENLEIVSKRWE 164 Query: 131 DIDVASMGWNQTFDLVFASM---IPIIHNWESVEKILQCA-RKFCYISMPVLHTEHSLVK 186 DID A + + +D+VFAS +P I +VEK+ + A ++ C + Sbjct: 165 DIDPAEL--SGPYDVVFASYSLGMPDIR--AAVEKMCKLATKRVCLYWFLGSSPWEQWMI 220 Query: 187 EIWPLVTGQSYQS 199 ++WP + GQ Y+S Sbjct: 221 DLWPALHGQEYRS 233 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 425,197,062 Number of extensions: 16080440 Number of successful extensions: 42083 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 42103 Number of HSP's successfully gapped: 18 Length of query: 297 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 172 Effective length of database: 417,850,203 Effective search space: 71870234916 Effective search space used: 71870234916 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)