BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0031.p (459 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_347519.1| Probable cation efflux pump (multidrug resistan... 418 e-115 ref|NP_563451.1| conserved hypothetical protein [Clostridium per... 405 e-112 ref|NP_781281.1| Na+ driven multidrug efflux pump (mviN domain) ... 404 e-111 ref|NP_562192.1| conserved hypothetical protein [Clostridium per... 380 e-104 ref|NP_811034.1| putative Na+-driven multidrug efflux pump [Bact... 350 4e-95 >ref|NP_347519.1| Probable cation efflux pump (multidrug resistance protein) [Clostridium acetobutylicum] pir||H97008 probable cation efflux pump (multidrug resistance protein) [imported] - Clostridium acetobutylicum gb|AAK78859.1| Probable cation efflux pump (multidrug resistance protein) [Clostridium acetobutylicum] Length = 448 Score = 418 bits (1075), Expect = e-115, Method: Compositional matrix adjust. Identities = 212/433 (48%), Positives = 314/433 (72%), Gaps = 8/433 (1%) Query: 4 LNE--QLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGR-LGALAMTGIGLTLPFM 60 +NE +L +E + KLLLKFSIP+I GM+V++LY+++DRIFVGR + + A++G+ +T P Sbjct: 1 MNEPNELETEGVWKLLLKFSIPSIIGMVVNALYSIVDRIFVGRGVNSTALSGVAITYPIS 60 Query: 61 TLLTAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFKEPIL 120 ++ AF L GIG SA+VS++LGQ + + AEK++GN F++L + LVVT GLIF EP+L Sbjct: 61 NVILAFGMLAGIGCSALVSIKLGQKKVKQAEKIIGNTFTLLIIFSLVVTFFGLIFLEPML 120 Query: 121 TLLGASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVLDMIL 180 LLGAS+ TM YA + IIL G + Q +G GL++ +RAEG P A ++IG VL+ I+ Sbjct: 121 KLLGASKNTMPYAKQFAVIILSGVILQNMGFGLNSTMRAEGDPKMAMKTMLIGAVLNFIM 180 Query: 181 NPLFIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVKDILS 240 NPLFIF +G+ G+A T++S V ++ V+ +F+ KS LK++ N++L +V++++S Sbjct: 181 NPLFIFVLKLGVRGSALATIVSQAVCSVWVLYYFIKGKSFLKIKRENIRLRREVVREVIS 240 Query: 241 IGLSPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGAQPIL 300 IG+SPF MQ++A ++ I N +L+ YGGD +I A ++++++ +L LM GI QG+QPI+ Sbjct: 241 IGMSPFAMQLAASLITITFNKSLEKYGGDTSIAAFSLISSIQMLILMPIFGINQGSQPII 300 Query: 301 GFNYGAQQYDRVKKTLKLELALVTAICV--LTFLMVQIFPTGLARILS-NDLELINAASH 357 G+NYGA+ RVKK L A+++A+C+ L F++VQIFPT + + + ND++LI + Sbjct: 301 GYNYGAKNIKRVKKA--LWYAVLSALCISFLGFIIVQIFPTQIISLFNKNDMKLIGVGTR 358 Query: 358 GMKIFLLMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGVW 417 G+ IFLLMLP+IG Q V +YFQAVG AKK+M L LLRQVI L+P LI+LP FFGL G+W Sbjct: 359 GISIFLLMLPVIGPQAVCTNYFQAVGKAKKSMLLSLLRQVIFLLPSLIVLPRFFGLNGIW 418 Query: 418 AAAPTSDFISCSV 430 AA PTSDF++ +V Sbjct: 419 AAGPTSDFLAFAV 431 >ref|NP_563451.1| conserved hypothetical protein [Clostridium perfringens] dbj|BAB82241.1| conserved hypothetical protein [Clostridium perfringens str. 13] Length = 455 Score = 405 bits (1042), Expect = e-112, Method: Compositional matrix adjust. Identities = 206/448 (45%), Positives = 323/448 (72%), Gaps = 5/448 (1%) Query: 8 LGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGRL---GALAMTGIGLTLPFMTLLT 64 LG E KLL+K+S+PAI GMLV++LYN++DRIF+G + G +A+TG+G+T+P M++L Sbjct: 7 LGEESELKLLIKYSVPAIIGMLVNALYNIVDRIFIGHIPGVGPMAITGVGITMPIMSILL 66 Query: 65 AFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFKEPILTLLG 124 F L+GIG +A +S++ GQN+++DAEK+LGN+ ++T++ LV+T +G++F IL L G Sbjct: 67 GFGMLIGIGATANISIKFGQNKRKDAEKILGNSVVLITIISLVLTIVGIVFANSILHLFG 126 Query: 125 ASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVLDMILNPLF 184 ASE T+ YA +YI +IL+G++F + L + IRA+G+P + V+V+G ++++IL+ +F Sbjct: 127 ASEATIFYAKEYINVILLGTIFNLMSFSLYSTIRADGNPKMSAAVMVLGCIINVILDAVF 186 Query: 185 IFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVKDILSIGLS 244 IF FN+GI GAA TV+S VTT++++ ++ S LKL+ LKL +VK +L+IG++ Sbjct: 187 IFVFNLGIKGAALATVISQIVTTLIMLYYYTLGGSNLKLKFETLKLDWRLVKIVLAIGVA 246 Query: 245 PFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGAQPILGFNY 304 PF MQ++A +V +I+NNAL+ YGGD+AIGAM ++++ ++FLM GI QG+QPI+G+NY Sbjct: 247 PFSMQMAASVVQVIANNALRMYGGDLAIGAMAAISSIAMIFLMPIFGINQGSQPIIGYNY 306 Query: 305 GAQQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAASHGMKIFLL 364 GA++Y+R +KT+KL + T I VL +++Q FP + + ++D +L+ G++I+L Sbjct: 307 GAKKYERAQKTVKLAMIAATTILVLGGILIQAFPALVISMFNSDPKLLEIGVPGLRIYLF 366 Query: 365 MLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNF--FGLTGVWAAAPT 422 M+PIIG I+G++YFQ++G AK A FL LLRQVI LIP+ ++LP GLTGVW A Sbjct: 367 MMPIIGISIIGSNYFQSIGKAKLATFLSLLRQVILLIPLTLVLPKIAGLGLTGVWLAGTV 426 Query: 423 SDFISCSVAIVFVAREIKRLGKAEQHQA 450 SDF+S + +F+ +E K+ E +A Sbjct: 427 SDFLSTIITGLFIIKEFKKEDSIEDEKA 454 >ref|NP_781281.1| Na+ driven multidrug efflux pump (mviN domain) [Clostridium tetani E88] gb|AAO35218.1| Na+ driven multidrug efflux pump (mviN domain) [Clostridium tetani E88] Length = 456 Score = 404 bits (1038), Expect = e-111, Method: Compositional matrix adjust. Identities = 215/444 (48%), Positives = 321/444 (72%), Gaps = 1/444 (0%) Query: 5 NEQLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGR-LGALAMTGIGLTLPFMTLL 63 ++QLG E + LL KFS+PAI GMLV++LYN++DR+F+GR +GALA++G+ + P + Sbjct: 8 SKQLGEEKVGTLLFKFSLPAIVGMLVNALYNMVDRVFIGRGVGALAISGLAVGFPLSIIG 67 Query: 64 TAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFKEPILTLL 123 AF LVGIG+S ++S++LG+ +K++AE++LGN+ ++ ++ + ++ +GLIF + IL + Sbjct: 68 MAFGMLVGIGSSTMISIKLGEKKKDEAERILGNSLILIILISICLSIVGLIFLDDILKIF 127 Query: 124 GASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVLDMILNPL 183 GAS T+ YA DY+ I+ G++ Q IG ++N+IRAEG+P A ++IG +++ IL+P+ Sbjct: 128 GASAETLPYARDYMKYIVAGALLQNIGFSMNNIIRAEGNPKIAMATMLIGAIINTILDPI 187 Query: 184 FIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVKDILSIGL 243 FIF F MGI GAA T+ + TV++I V+ +F +S LK+R NL L +K I+SIG+ Sbjct: 188 FIFVFKMGIKGAALATIFAQTVSSIWVLYYFFSGRSTLKIRRENLNLHKGTIKTIMSIGI 247 Query: 244 SPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGAQPILGFN 303 SPF MQI+A +V I N L T+GGD+A+GAM +VN++ +LF M GI QG QPI+G+N Sbjct: 248 SPFSMQIAASLVTTIFNKNLLTHGGDLAVGAMGIVNSISMLFFMPMFGINQGMQPIIGYN 307 Query: 304 YGAQQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAASHGMKIFL 363 YGA+QY RV+KTLKL + I + F++V++FPT L +I + D +L++ ++GM+IFL Sbjct: 308 YGAKQYKRVRKTLKLAITSAMIIATIGFIVVEVFPTALIKIFNKDEQLVSIGAYGMRIFL 367 Query: 364 LMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGVWAAAPTS 423 MLPIIG Q+V ++YFQAVG AK A+FL LLRQVI LIPML+ILP FGL GVW P S Sbjct: 368 SMLPIIGFQVVSSNYFQAVGKAKIAIFLSLLRQVILLIPMLLILPPIFGLNGVWIVGPVS 427 Query: 424 DFISCSVAIVFVAREIKRLGKAEQ 447 D ++ V + F+++ +K+L K E+ Sbjct: 428 DVLAAIVTVFFLSKNMKQLMKLEK 451 >ref|NP_562192.1| conserved hypothetical protein [Clostridium perfringens] dbj|BAB80982.1| conserved hypothetical protein [Clostridium perfringens str. 13] Length = 456 Score = 380 bits (976), Expect = e-104, Method: Compositional matrix adjust. Identities = 191/435 (43%), Positives = 297/435 (68%), Gaps = 3/435 (0%) Query: 7 QLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGRL---GALAMTGIGLTLPFMTLL 63 +L +E I +LLLK+S+PAI M+V+SLYN +DR F+G + GALA++G+G+T+P T+L Sbjct: 6 ELKNEKIGRLLLKYSLPAILSMMVTSLYNTVDRAFIGSIKDIGALAISGLGVTMPLFTIL 65 Query: 64 TAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFKEPILTLL 123 AF + +G S +S++LG+ ++E+AE++LGN F + V+ L++ GL F E IL Sbjct: 66 GAFCVAISVGGSTNISIKLGEEKREEAERILGNTFILSIVVALIIMIFGLSFLEKILYFF 125 Query: 124 GASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVLDMILNPL 183 AS+ T+ YA DY+ +ILIG+ F G L+N IRAEG+P A +++I VL++IL+P+ Sbjct: 126 EASKETIIYAKDYMRVILIGAWFNFPGFALNNAIRAEGNPKLAGKIMIISCVLNIILDPI 185 Query: 184 FIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVKDILSIGL 243 FIF F+MGI GAA T++ + + + +F KS LKLRI L+ I++ I+ I L Sbjct: 186 FIFLFHMGIKGAALGTIICQFIVFLWTMYYFTLGKSNLKLRIKGKILNKNILRAIILIAL 245 Query: 244 SPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGAQPILGFN 303 +PFFM+++A + +I+N LK YGGD+AIGAMT + ++ +LFLM G++QG Q I+ +N Sbjct: 246 TPFFMELAAGFIHLITNRVLKDYGGDLAIGAMTSITSIYLLFLMPVFGLSQGMQTIVAYN 305 Query: 304 YGAQQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAASHGMKIFL 363 YGA++Y R KKTL + + + T I L ++++I+P I + D ELIN A +G+KI+ Sbjct: 306 YGAKEYKRAKKTLLMTIIIATLILTLGLVLIRIYPREFINIFTKDKELINIALNGLKIYT 365 Query: 364 LMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGVWAAAPTS 423 + LP +G I+GA YFQ++G KK++FL LLRQVI IP++ I+P +GL GVW + P + Sbjct: 366 IALPTLGVSILGAVYFQSIGNVKKSIFLSLLRQVILFIPIVFIIPRVYGLNGVWISQPLA 425 Query: 424 DFISCSVAIVFVARE 438 D ++ + ++F+ +E Sbjct: 426 DILATLIILIFLIKE 440 >ref|NP_811034.1| putative Na+-driven multidrug efflux pump [Bacteroides thetaiotaomicron VPI-5482] gb|AAO77228.1| putative Na+-driven multidrug efflux pump [Bacteroides thetaiotaomicron VPI-5482] Length = 450 Score = 350 bits (898), Expect = 4e-95, Method: Compositional matrix adjust. Identities = 183/439 (41%), Positives = 278/439 (63%), Gaps = 1/439 (0%) Query: 8 LGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGR-LGALAMTGIGLTLPFMTLLTAF 66 LG+E I KLL++++IPAI M SSLYN++D IF+G +GA+A++G+ LT P M L AF Sbjct: 10 LGTEKIGKLLMQYAIPAIIAMTASSLYNMVDSIFIGHGVGAMAISGLALTFPLMNLAAAF 69 Query: 67 TSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFKEPILTLLGAS 126 SLVG+G + +VS++LGQ + A++VLGN + ++ L T + LIF +PIL G S Sbjct: 70 GSLVGVGAATLVSVKLGQKDYDTAQRVLGNVLVLNIIIGLAFTVLTLIFLDPILYFFGGS 129 Query: 127 ELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVLDMILNPLFIF 186 E T+GYA DY+ +IL G+V + GL+ V+R+ GHP KA + V++ IL+P+FI+ Sbjct: 130 EATVGYARDYMVVILWGNVITHLYLGLNAVLRSAGHPQKAMYATIATVVINTILDPIFIY 189 Query: 187 TFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVKDILSIGLSPF 246 F GI GAA T+ + + + F K +L +L IV D L+IG++PF Sbjct: 190 GFGWGIQGAAIATITAQVIALLWQFKLFSNKDELLHFHKGIFRLKKKIVFDSLAIGMAPF 249 Query: 247 FMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGAQPILGFNYGA 306 M ++A + I+ N LK +GGD+AIGA +VN ++ LF+M MG+ QG QPI G+N+GA Sbjct: 250 LMNLAACFIVILINKGLKQHGGDLAIGAFGIVNRLVFLFVMIVMGLNQGMQPIAGYNFGA 309 Query: 307 QQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAASHGMKIFLLML 366 +QY RV + LK+ + T + + FLM P I + EL+N ++ G++I ++ Sbjct: 310 KQYPRVTQVLKITIYAATIVTTIGFLMGMFIPQLAVSIFTTHEELVNISAKGLRIVVMFF 369 Query: 367 PIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGVWAAAPTSDFI 426 PI+G Q+V +++FQ++G A KA+FL + RQV+ LIP L+ILP F+G GVW + P SD I Sbjct: 370 PIVGFQMVTSNFFQSIGMASKAIFLSISRQVLVLIPCLLILPRFYGQLGVWISMPISDLI 429 Query: 427 SCSVAIVFVAREIKRLGKA 445 + +A + + ++ KA Sbjct: 430 ASLIAGGMLWWQFRQFRKA 448 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.327 0.141 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 587,122,127 Number of extensions: 23083808 Number of successful extensions: 94878 Number of sequences better than 1.0e-05: 740 Number of HSP's gapped: 93979 Number of HSP's successfully gapped: 749 Length of query: 459 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 330 Effective length of database: 406,989,807 Effective search space: 134306636310 Effective search space used: 134306636310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 128 (53.9 bits)