BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0008.p (185 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAJ08049.1| unknown [environmental sequence] 91 1e-17 ref|NP_243627.1| branched-chain alpha-keto acid dehydrogenase E2... 91 2e-17 ref|NP_390283.1| branched-chain alpha-keto acid dehydrogenase E2... 90 3e-17 ref|NP_764751.1| branched-chain alpha-keto acid dehydrogenase E2... 89 4e-17 ref|ZP_00018657.1| hypothetical protein [Chloroflexus aurantiacus] 87 2e-16 >gb|EAJ08049.1| unknown [environmental sequence] Length = 186 Score = 90.9 bits (224), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 38/67 (56%), Positives = 53/67 (79%) Query: 12 MPQLAESLVSATIGKWLKQPGDAIEQYEPLCDLITDKVNAELPATVDGTLVELLAEEGQT 71 +PQ+ ES+V TI KWLK PGD I+QYEPL +++TDKV E+P+ ++GT+ + LAEEG+T Sbjct: 9 LPQVGESIVEGTIAKWLKHPGDTIKQYEPLVEVVTDKVTMEVPSPIEGTISKFLAEEGET 68 Query: 72 VSVGEVI 78 V+VG I Sbjct: 69 VAVGSPI 75 >ref|NP_243627.1| branched-chain alpha-keto acid dehydrogenase E2 [Bacillus halodurans] pir||A83995 branched-chain alpha-keto acid dehydrogenase E2 bfmBB [imported] - Bacillus halodurans (strain C-125) dbj|BAB06480.1| branched-chain alpha-keto acid dehydrogenase E2 [Bacillus halodurans] Length = 426 Score = 90.5 bits (223), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 38/71 (53%), Positives = 55/71 (77%) Query: 8 TDVIMPQLAESLVSATIGKWLKQPGDAIEQYEPLCDLITDKVNAELPATVDGTLVELLAE 67 T++ MPQL ES+ TI +WL +PGD + +Y+PL +++TDKVNAE+P++ GT+ ELL E Sbjct: 3 TEITMPQLGESVTEGTISRWLVKPGDKVNKYDPLAEVLTDKVNAEIPSSFSGTIQELLVE 62 Query: 68 EGQTVSVGEVI 78 E +TV+VG VI Sbjct: 63 EDETVAVGHVI 73 >ref|NP_390283.1| branched-chain alpha-keto acid dehydrogenase E2 subunit (lipoamide acyltransferase) [Bacillus subtilis] sp|P37942|ODB2_BACSU Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase) (E2) (Dihydrolipoamide branched chain transacylase) pir||S32488 dihydrolipoamide S-acyltransferase (EC 2.3.1.-), alpha-oxo acid dehydrogenase complex (bfmBB) - Bacillus subtilis gb|AAA22280.1| branched chain alpha-keto acid dehydrogenase E2 dbj|BAA12600.1| BfmBB [Bacillus subtilis] emb|CAB14334.1| branched-chain alpha-keto acid dehydrogenase E2 subunit (lipoamide acyltransferase) [Bacillus subtilis subsp. subtilis str. 168] Length = 424 Score = 89.7 bits (221), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 37/72 (51%), Positives = 54/72 (75%) Query: 7 LTDVIMPQLAESLVSATIGKWLKQPGDAIEQYEPLCDLITDKVNAELPATVDGTLVELLA 66 + + MPQL ES+ TI KWL PGD + +Y+P+ +++TDKVNAE+P++ GT+ EL+ Sbjct: 3 IEQMTMPQLGESVTEGTISKWLVAPGDKVNKYDPIAEVMTDKVNAEVPSSFTGTITELVG 62 Query: 67 EEGQTVSVGEVI 78 EEGQT+ VGE+I Sbjct: 63 EEGQTLQVGEMI 74 >ref|NP_764751.1| branched-chain alpha-keto acid dehydrogenase E2 [Staphylococcus epidermidis ATCC 12228] gb|AAO04795.1| branched-chain alpha-keto acid dehydrogenase E2 [Staphylococcus epidermidis ATCC 12228] Length = 439 Score = 89.4 bits (220), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 38/70 (54%), Positives = 54/70 (77%) Query: 9 DVIMPQLAESLVSATIGKWLKQPGDAIEQYEPLCDLITDKVNAELPATVDGTLVELLAEE 68 D+ MP+L ES+ TI +WL GD +++YEPLC++ITDKV AE+P+T+ GT+ EL+ EE Sbjct: 2 DIKMPKLGESVHEGTIEQWLVSVGDHVDEYEPLCEVITDKVTAEVPSTISGTITELVVEE 61 Query: 69 GQTVSVGEVI 78 GQTV++ VI Sbjct: 62 GQTVNINTVI 71 >ref|ZP_00018657.1| hypothetical protein [Chloroflexus aurantiacus] Length = 447 Score = 87.0 bits (214), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 38/72 (52%), Positives = 53/72 (73%) Query: 7 LTDVIMPQLAESLVSATIGKWLKQPGDAIEQYEPLCDLITDKVNAELPATVDGTLVELLA 66 + D+ MPQL ES+ T+G+WLK+PGD + +YEPL +++TDKV+ E+PA G L E+L Sbjct: 4 MIDIKMPQLGESVTEGTVGRWLKRPGDPVAKYEPLLEVVTDKVDTEVPAPEAGVLHEILV 63 Query: 67 EEGQTVSVGEVI 78 EG+TV VG VI Sbjct: 64 PEGETVRVGTVI 75 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.131 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 165,684,794 Number of extensions: 4741306 Number of successful extensions: 15458 Number of sequences better than 1.0e-05: 580 Number of HSP's gapped: 15412 Number of HSP's successfully gapped: 606 Length of query: 185 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 67 Effective length of database: 436,855,896 Effective search space: 29269345032 Effective search space used: 29269345032 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 122 (51.6 bits)