BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0002.p (221 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAD11095.1| lipoamide dehydrogenase of 2-oxo acid dehydrogen... 236 2e-61 ref|NP_390286.1| similar to dihydrolipoamide dehydrogenase [Baci... 236 4e-61 sp|P54533|DLD2_BACSU Dihydrolipoyl dehydrogenase (E3 component o... 235 4e-61 dbj|BAD11090.1| lipoamide dehydrogenase of 2-oxo acid dehydrogen... 234 9e-61 ref|NP_833875.1| Dihydrolipoamide dehydrogenase [Bacillus cereus... 230 1e-59 >dbj|BAD11095.1| lipoamide dehydrogenase of 2-oxo acid dehydrogenase [Geobacillus thermocatenulatus] Length = 476 Score = 236 bits (603), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 121/218 (55%), Positives = 150/218 (68%), Gaps = 1/218 (0%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 M DV ILGGGTGGYVAAIRA+QLG V+E KLGGTCLH GCIPSK+LLRSAEVY Sbjct: 1 MAKEYDVVILGGGTGGYVAAIRASQLGLKTAVVEKGKLGGTCLHAGCIPSKALLRSAEVY 60 Query: 61 AEIQESESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPS 120 A+ + E++G+ GV+L F KVQARK A+V+QLH+GVQ LM+K KI V G GR++GPS Sbjct: 61 AQTKNGEAFGVIADGVRLDFAKVQARKAAIVDQLHKGVQHLMKKGKIDVFAGIGRLLGPS 120 Query: 121 IFSPRSGAVAVELENG-EMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXX 179 IFSP G V+VE+ +G E E +V +++ATGSRPR LPGL+PDG+ + Sbjct: 121 IFSPLPGTVSVEMNDGSENEMLVPKFVVIATGSRPRTLPGLEPDGEFVMTSDEALQMETL 180 Query: 180 XXXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 EWASML DFGV+VTV+E A ++LP Sbjct: 181 PSSILIVGGGVIGMEWASMLNDFGVEVTVLEYADRILP 218 >ref|NP_390286.1| similar to dihydrolipoamide dehydrogenase [Bacillus subtilis] pir||D69962 dihydrolipoamide dehydrogenase homolog yqiV - Bacillus subtilis dbj|BAA12597.1| YqiV [Bacillus subtilis] emb|CAB14337.1| branched-chain alpha-keto acid dehydrogenase E3 subunit (dihydrolipoamide dehydrogenase) [Bacillus subtilis subsp. subtilis str. 168] Length = 457 Score = 236 bits (601), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 121/218 (55%), Positives = 149/218 (68%), Gaps = 1/218 (0%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 M DV ILGGGTGGYVAAIRAAQLG V+E +KLGGTCLH+GCIPSK+LLRSAEVY Sbjct: 1 MATEYDVVILGGGTGGYVAAIRAAQLGLKTAVVEKEKLGGTCLHKGCIPSKALLRSAEVY 60 Query: 61 AEIQESESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPS 120 +E++ +G+ETAGV L F KVQ RK+AVV++L GV LM+K KI V G GRI+GPS Sbjct: 61 RTAREADQFGVETAGVSLNFEKVQQRKQAVVDKLAAGVNHLMKKGKIDVYTGYGRILGPS 120 Query: 121 IFSPRSGAVAVELENG-EMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXX 179 IFSP G ++VE NG E + ++ +I+ATGSRPR+LPGL+ DGK + Sbjct: 121 IFSPLPGTISVERGNGEENDMLIPKQVIIATGSRPRMLPGLEVDGKSVLTSDEALQMEEL 180 Query: 180 XXXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 EWASML DFGV+VTV+E A ++LP Sbjct: 181 PQSIIIVGGGVIGIEWASMLHDFGVKVTVIEYADRILP 218 >sp|P54533|DLD2_BACSU Dihydrolipoyl dehydrogenase (E3 component of branched-chain alpha-keto acid dehydrogenase complex) (LPD-Val) (Dihydrolipoamide dehydrogenase) Length = 474 Score = 235 bits (600), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 121/218 (55%), Positives = 149/218 (68%), Gaps = 1/218 (0%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 M DV ILGGGTGGYVAAIRAAQLG V+E +KLGGTCLH+GCIPSK+LLRSAEVY Sbjct: 1 MATEYDVVILGGGTGGYVAAIRAAQLGLKTAVVEKEKLGGTCLHKGCIPSKALLRSAEVY 60 Query: 61 AEIQESESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPS 120 +E++ +G+ETAGV L F KVQ RK+AVV++L GV LM+K KI V G GRI+GPS Sbjct: 61 RTAREADQFGVETAGVSLNFEKVQQRKQAVVDKLAAGVNHLMKKGKIDVYTGYGRILGPS 120 Query: 121 IFSPRSGAVAVELENG-EMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXX 179 IFSP G ++VE NG E + ++ +I+ATGSRPR+LPGL+ DGK + Sbjct: 121 IFSPLPGTISVERGNGEENDMLIPKQVIIATGSRPRMLPGLEVDGKSVLTSDEALQMEEL 180 Query: 180 XXXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 EWASML DFGV+VTV+E A ++LP Sbjct: 181 PQSIIIVGGGVIGIEWASMLHDFGVKVTVIEYADRILP 218 >dbj|BAD11090.1| lipoamide dehydrogenase of 2-oxo acid dehydrogenase [Moorella thermoacetica] Length = 473 Score = 234 bits (597), Expect = 9e-61, Method: Compositional matrix adjust. Identities = 120/218 (55%), Positives = 149/218 (68%), Gaps = 1/218 (0%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 M DV ILGGGTGGYVAAIRA+QLG V+E KLGGTCLH GCIPSK+LLRSAEVY Sbjct: 1 MAKEYDVVILGGGTGGYVAAIRASQLGLKTAVVEKGKLGGTCLHAGCIPSKALLRSAEVY 60 Query: 61 AEIQESESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPS 120 A+ + SE++G+ V+L F KVQARK +V+QLH+GVQ LM+K KI V G GR++GPS Sbjct: 61 AQTKNSEAFGVIVGDVRLDFAKVQARKATIVDQLHKGVQHLMKKGKIDVYAGIGRLLGPS 120 Query: 121 IFSPRSGAVAVELENG-EMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXX 179 IFSP G V+VE+ +G E E +V +++ATGSRPR LPGL+PDG+ + Sbjct: 121 IFSPLPGTVSVEMNDGSENEMLVPKFVVIATGSRPRTLPGLEPDGEFVMTSDEALQMEVL 180 Query: 180 XXXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 EWASML DFGV+VTV+E A ++LP Sbjct: 181 PSSILIVGGGVIGMEWASMLNDFGVEVTVLEYADRILP 218 >ref|NP_833875.1| Dihydrolipoamide dehydrogenase [Bacillus cereus ATCC 14579] gb|AAP11076.1| Dihydrolipoamide dehydrogenase [Bacillus cereus ATCC 14579] Length = 473 Score = 230 bits (587), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 115/218 (52%), Positives = 149/218 (68%), Gaps = 1/218 (0%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 M D+ I+GGGTGGYVAAIRA+QLG ++E + LGGTCLH+GCIPSK+LLRSAEVY Sbjct: 1 MAKEYDLVIVGGGTGGYVAAIRASQLGLKTALVEKENLGGTCLHKGCIPSKALLRSAEVY 60 Query: 61 AEIQESESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPS 120 A ++SE +G+ + V+L F KVQ RKE +V QLH+GVQ LM++ KI V +G GRI+GPS Sbjct: 61 ATAKKSEEFGVIASNVELNFAKVQERKEKIVTQLHKGVQHLMKQGKIDVFEGIGRILGPS 120 Query: 121 IFSPRSGAVAVELENG-EMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXX 179 IFSP G ++VEL +G E E ++ N+++ATGSRP LPGL+ DG+ + Sbjct: 121 IFSPMPGTISVELASGEENEMLIPKNVLIATGSRPNSLPGLELDGEYVMSSDHALKMETL 180 Query: 180 XXXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 EWASMLADFGV+VTV+E A +LP Sbjct: 181 PSSIIIVGGGVIGIEWASMLADFGVEVTVLEYAKTILP 218 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 261,426,391 Number of extensions: 9174023 Number of successful extensions: 40587 Number of sequences better than 1.0e-05: 1432 Number of HSP's gapped: 38353 Number of HSP's successfully gapped: 1432 Length of query: 221 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 100 Effective length of database: 428,710,599 Effective search space: 42871059900 Effective search space used: 42871059900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)