BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0385.0012.p (333 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_229030.1| conserved hypothetical protein [Thermotoga mari... 163 7e-39 ref|NP_809946.1| conserved hypothetical protein [Bacteroides the... 160 3e-38 ref|ZP_00059726.1| COG2152: Predicted glycosylase [Clostridium t... 159 8e-38 ref|ZP_00076614.1| COG2152: Predicted glycosylase [Methanosarcin... 144 2e-33 ref|NP_406008.1| conserved hypothetical protein [Yersinia pestis... 142 1e-32 >ref|NP_229030.1| conserved hypothetical protein [Thermotoga maritima] pir||B72278 conserved hypothetical protein - Thermotoga maritima (strain MSB8) gb|AAD36300.1| conserved hypothetical protein [Thermotoga maritima] Length = 326 Score = 163 bits (412), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 119/326 (36%), Positives = 168/326 (51%), Gaps = 34/326 (10%) Query: 15 IHRYEGNPVLDASKVPYNTALVFNAGVTKFKGKYVMVFRNDYGSLADQTIEPHHTTDLGI 74 + RY NP++ + VP A VFN+ V + G++V VFR D+ + P L Sbjct: 24 VWRYSKNPIIGRNPVP-KGARVFNSAVVPYNGEFVGVFRIDH-----KNTRPF----LHF 73 Query: 75 AFSDDGIHWEASPKKC--FKLHDE--EIIRAYDPRLTVIGDRCYMCFAVDTRHGIRGGIA 130 S DGI+WE P++ ++ E + AYDPR+ I D Y+ F D HG G+ Sbjct: 74 GRSKDGINWEIEPEEIQWVDVNGEPFQPSYAYDPRVVKIEDTYYITFCTDD-HGPTIGVG 132 Query: 131 VTDDFEEFEIL-SLSSPDLRNMVLFPEKIGGNYVRLERPFTVYSRGGKDRF-DTWISESP 188 +T DF+ F L + P RN VLFP KI G YV L RP S G F D ++SESP Sbjct: 133 MTKDFKTFVRLPNAYVPFNRNGVLFPRKINGKYVMLNRP----SDNGHTPFGDIFLSESP 188 Query: 189 DLKYWGNSDLLLA-VEHVPFANDKVGPAAPPVKTDKGWLTTFHAVDIDPSRGKHGWEPAW 247 D+ +WGN +L + + N K+G P++T +GWL +H V + + Sbjct: 189 DMIHWGNHRFVLGRSSYNWWENLKIGAGPYPIETSEGWLLIYHGVTLTCN---------- 238 Query: 248 KKRYTAGIMLLDLDNPKKILGMYKQPLLAPETSYEIDGGFRNNVIFPGGMILE-DNGEVK 306 Y+ G LLDLD+P K+L + LL PE YE GF NV+FP + + D G V Sbjct: 239 GYVYSFGAALLDLDDPSKVLYRSRYYLLTPEEEYETV-GFVPNVVFPCAALCDADTGRVA 297 Query: 307 IYYGSADTIECLATAHVDDLVSLCLK 332 IYYG+ADT LA ++D++V + Sbjct: 298 IYYGAADTHVALAFGYIDEIVDFVKR 323 >ref|NP_809946.1| conserved hypothetical protein [Bacteroides thetaiotaomicron VPI-5482] gb|AAO76140.1| conserved hypothetical protein [Bacteroides thetaiotaomicron VPI-5482] Length = 322 Score = 160 bits (406), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 114/325 (35%), Positives = 168/325 (51%), Gaps = 36/325 (11%) Query: 14 VIHRYEGNPVLDASKVPYNTALVFNAGVTKFKGKYVMVFRNDYGSLADQTIEPHHTTDLG 73 V+ RY NPV+ +P + + +FN+ V FK + VFR D ++ ++ Sbjct: 18 VMWRYSQNPVIGRYHIPSSNS-IFNSAVVPFKDGFAGVFRCDNKAV---------QMNIF 67 Query: 74 IAFSDDGIHWEASPKKC-FKLHDEEIIRA---YDPRLTVIGDRCYMCFAVDTRHGIRGGI 129 FS DGIHW+ S + FK + E+I + YDPR+T I DR ++ + + HG GI Sbjct: 68 TGFSKDGIHWDISHEPIQFKAGNTEMIESEYKYDPRVTWIEDRYWVTWC-NGYHGPTIGI 126 Query: 130 AVTDDFEEF-EILSLSSPDLRNMVLFPEKIGGNYVRLERPFTVYSRGGKDRF-DTWISES 187 A T DF +F + + P RN VLFP+KI G Y L RP S G F D +IS S Sbjct: 127 AYTFDFVDFFQCENAFLPFNRNGVLFPQKIDGKYAMLSRP----SDNGHTPFGDIYISYS 182 Query: 188 PDLKYWGNSDLLLAVEHVP---FANDKVGPAAPPVKTDKGWLTTFHAVDIDPSRGKHGWE 244 PD+KYWG ++ V P + K+G + P TD+GWL +H V I G Sbjct: 183 PDMKYWGEHRCVMKVTPFPESAWQCTKIGAGSVPFLTDEGWLLFYHGV-ITTCNGF---- 237 Query: 245 PAWKKRYTAGIMLLDLDNPKKILGMYKQPLLAPETSYEIDGGFRNNVIFPGGMILEDNGE 304 RY G +LD D+P+K+L ++ L+ P YE+ G NV+FP L+D Sbjct: 238 -----RYAMGSAILDKDHPEKVLYRTREYLIGPAAPYELQGDVP-NVVFPCAA-LQDGER 290 Query: 305 VKIYYGSADTIECLATAHVDDLVSL 329 V +YYG+ADT+ +A ++ +++ Sbjct: 291 VAVYYGAADTVVGMAFGYIQEIIDF 315 >ref|ZP_00059726.1| COG2152: Predicted glycosylase [Clostridium thermocellum ATCC 27405] Length = 334 Score = 159 bits (402), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 111/328 (33%), Positives = 170/328 (51%), Gaps = 41/328 (12%) Query: 14 VIHRYEGNPVLDASKVPYNTALVFNAGVTKFKGKYVMVFRNDYGSLADQTIEPHHTTDLG 73 + R+ GNP+L A PY VFN T + GK +++ R + D+ H T Sbjct: 24 LFKRFPGNPILSAKNWPYAANTVFNPAATMYNGKVLLLIR-----VEDRRGFSHLTK--- 75 Query: 74 IAFSDDGI-HW--------EASPKKCFKLHDEEIIRAYDPRLTVIGD--RCYMCFAVDTR 122 A SDDGI +W EA P+K EE DPR+T I + + + + ++ Sbjct: 76 -AISDDGISNWIIDDKPTLEAEPEK----FPEEEWGVEDPRITWIEELGKFAVVYTAYSK 130 Query: 123 HGIRGGIAVTDDFEEFEIL-SLSSPDLRNMVLFPEKIGGNYVRLERPFTVYSRGGKDRFD 181 G +A+T+DFE FE L ++ P+ ++ LFP +I G +V + RP +++ G Sbjct: 131 GGPLVSLALTEDFENFEKLGAIMPPEDKDAALFPRRINGKWVLIHRPISIHHGPGAH--- 187 Query: 182 TWISESPDLKYWGNSDLLLAVEHVPFAN-DKVGPAAPPVKTDKGWLTTFHAVDIDPSRGK 240 WIS S DLKYWG+ +L+ + + +KVG PP++T GWL +H V Sbjct: 188 IWISRSDDLKYWGDHQILIRARKGGWWDANKVGLNCPPLETPDGWLILYHGVR------- 240 Query: 241 HGWEPAWKKRYTAGIMLLDLDNPKKILGMYKQPLLAPETSYEIDGGFRNNVIFPGGMIL- 299 + A Y G+ LLDL+NP K+L + + P+ YE +G ++V+FP G + Sbjct: 241 ---QTASGSIYRLGLALLDLENPSKVLRRSDEWVFGPQEFYEREGDV-DDVVFPCGWVYN 296 Query: 300 EDNGEVKIYYGSADTIECLATAHVDDLV 327 E GE+KIYYG+ADT +AT ++ DL+ Sbjct: 297 EKTGEIKIYYGAADTCIAMATTNISDLL 324 >ref|ZP_00076614.1| COG2152: Predicted glycosylase [Methanosarcina barkeri] Length = 324 Score = 144 bits (364), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 104/322 (32%), Positives = 158/322 (49%), Gaps = 35/322 (10%) Query: 17 RYEGNPVLDASKVPYNTALVFNAGVTKFKGKYVMVFRNDYGSLADQTIEPHHT-TDLGIA 75 RY+ NP+L A PY + VFN +++ R E H + L +A Sbjct: 16 RYKKNPILTADYWPYKVSSVFNPAAVMVDNTTILLVR----------AEDHRGFSHLTVA 65 Query: 76 FSDDGIH-WEASPKKCFK----LHDEEIIRAYDPRLTVIGD--RCYMCFAVDTRHGIRGG 128 S +GI WE PK F + EE+ DPR+T I + + + + + G Sbjct: 66 RSKNGIDGWEIDPKPTFTSDPTYYSEEVYGIEDPRITYIDEMGKWAIAYTAFSDSGPLTS 125 Query: 129 IAVTDDFEEFE-ILSLSSPDLRNMVLFPEKIGGNYVRLERPFTVYSRGGKDRFDTWISES 187 +A T++F FE + + P+ ++ +FP ++ G + L RP S + + WIS S Sbjct: 126 LAYTENFTNFERVGPIMPPENKDAAIFPTRLNGRWAMLHRPV---SNIAGAKANLWISFS 182 Query: 188 PDLKYWGNSDLLL-AVEHVPFANDKVGPAAPPVKTDKGWLTTFHAVDIDPSRGKHGWEPA 246 PD+KYWG +LL A E + K+G A PP+KT GWL ++ V S Sbjct: 183 PDMKYWGEHKVLLYAREGGWWDARKIGLAPPPMKTSDGWLILYYGVRQTTS--------- 233 Query: 247 WKKRYTAGIMLLDLDNPKKILGMYKQPLLAPETSYEIDGGFRNNVIFPGGMILEDNGEVK 306 KK Y G+ LLDL++P K+L + + P YE G N+V+FP G IL + E++ Sbjct: 234 -KKSYRLGLALLDLEDPTKVLHRSEGWVFGPREMYERTGDV-NDVVFPCGWILAGD-ELR 290 Query: 307 IYYGSADTIECLATAHVDDLVS 328 IYYGSAD +A+A + D++ Sbjct: 291 IYYGSADMSVAMASAKMSDVMQ 312 >ref|NP_406008.1| conserved hypothetical protein [Yersinia pestis] ref|NP_669032.1| hypothetical [Yersinia pestis KIM] ref|NP_993622.1| Predicted glycosylase [Yersinia pestis biovar Mediaevails str. 91001] pir||AC0302 conserved hypothetical protein YPO2474 [imported] - Yersinia pestis (strain CO92) emb|CAC91279.1| conserved hypothetical protein [Yersinia pestis CO92] gb|AAM85283.1| hypothetical [Yersinia pestis KIM] gb|AAS62499.1| Predicted glycosylase [Yersinia pestis biovar Mediaevails str. 91001] Length = 351 Score = 142 bits (358), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 109/355 (30%), Positives = 160/355 (45%), Gaps = 71/355 (20%) Query: 17 RYEGNPVLDASKVP-----YNTALVFNAGVTKFKGKYVM--------------------- 50 RY GNP++ + + FNAG+TK + +M Sbjct: 3 RYSGNPLITPQDITPSHPGFKVECAFNAGITKLGDETIMLIRIAESVIPESEGITVVPLL 62 Query: 51 ------------VFRND-----------YGSLAD-QTIEPHHTTDLGIAFSDDGIHWEAS 86 VF++D +AD T+ + L +A S DG+ + Sbjct: 63 MEISGHWQVTTRVFKHDDPAYDFSDPRLISDIADPSTVYLTSLSHLRVARSHDGVTFVVD 122 Query: 87 PKK-CFKLHDEEIIRAYDPRLTVIGDRCYMCFAVDTRHGIRGGIAVTDDFEEFEILSLS- 144 + F + +E D R+T I Y+ ++ + GI +AVT DF + + L L Sbjct: 123 DQPFIFPANGDEAFGCEDARITQIDGAYYINYSAVSAKGICTALAVTQDFTDVKRLGLIF 182 Query: 145 SPDLRNMVLFPEKIGGNYVRLERPFTVYSRGGKDRFDTWISESPDLKYWGNSDLLLAVEH 204 PD R+ LFPEKI G Y+ L RP GK + W++ S DL +WG+ LL Sbjct: 183 CPDNRDACLFPEKINGKYMTLHRP--APKHFGKP--EIWLASSLDLLHWGDHRHLLGTSQ 238 Query: 205 VPFANDKVGPAAPPVKTDKGWLTTFHAVDIDPSRGKHGWEPAWKKRYTAGIMLLDLDNPK 264 P+ K+G A +KT+KGWL +H VD +RY+ G +LLDL+ P Sbjct: 239 DPWDALKLGGGAQMLKTEKGWLQIYHGVDA-------------TQRYSLGALLLDLEQPT 285 Query: 265 KILGMYKQPLLAPETSYEIDGGFRNNVIFPGGMILEDNGEVKIYYGSADTIECLA 319 KIL PLL P+ YE+ GF NV+F G ++E N +++YYG+AD CLA Sbjct: 286 KILAKSPVPLLQPQAPYELH-GFFGNVVFTCGALIE-NDSLRVYYGAADECMCLA 338 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.140 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 561,838,747 Number of extensions: 25256880 Number of successful extensions: 42955 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 42837 Number of HSP's successfully gapped: 32 Length of query: 333 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 207 Effective length of database: 415,135,104 Effective search space: 85932966528 Effective search space used: 85932966528 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)