BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0385.0004.p (337 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_229030.1| conserved hypothetical protein [Thermotoga mari... 170 5e-41 ref|ZP_00059726.1| COG2152: Predicted glycosylase [Clostridium t... 162 1e-38 ref|NP_617288.1| conserved hypothetical protein [Methanosarcina ... 155 2e-36 ref|ZP_00076614.1| COG2152: Predicted glycosylase [Methanosarcin... 154 3e-36 ref|NP_634968.1| conserved protein [Methanosarcina mazei Goe1] >... 152 1e-35 >ref|NP_229030.1| conserved hypothetical protein [Thermotoga maritima] pir||B72278 conserved hypothetical protein - Thermotoga maritima (strain MSB8) gb|AAD36300.1| conserved hypothetical protein [Thermotoga maritima] Length = 326 Score = 170 bits (430), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 122/322 (37%), Positives = 171/322 (53%), Gaps = 36/322 (11%) Query: 15 ITRHPANPILAPGDVPYGPAMVFNAGVTKFKGKYVMVFRNDYGDEAKGIVAPFHTTNLGL 74 + R+ NPI+ VP G A VFN+ V + G++V VFR D+ + PF L Sbjct: 24 VWRYSKNPIIGRNPVPKG-ARVFNSAVVPYNGEFVGVFRIDHKN-----TRPF----LHF 73 Query: 75 AFSDDGIKWEVQPEPCWSWHDE-----EVIRVYDPRLTVIGGQCYMCFAVDTKHGLRGGI 129 S DGI WE++PE W D + YDPR+ I Y+ F D HG G+ Sbjct: 74 GRSKDGINWEIEPEEI-QWVDVNGEPFQPSYAYDPRVVKIEDTYYITFCTD-DHGPTIGV 131 Query: 130 AVTEDFRSFEVL-SLSLPDNRNMVLFPELIGGKYVRLERPLPVYSRGGIDRF-DMWMSDS 187 +T+DF++F L + +P NRN VLFP I GKYV L RP S G F D+++S+S Sbjct: 132 GMTKDFKTFVRLPNAYVPFNRNGVLFPRKINGKYVMLNRP----SDNGHTPFGDIFLSES 187 Query: 188 PDLKYWGNSRLLLTVEEVAY-ANDKVGPGAPPVKTDKGWLTLFHAVDLDRSRGKNGWEDS 246 PD+ +WGN R +L + N K+G G P++T +GWL ++H V L NG+ Sbjct: 188 PDMIHWGNHRFVLGRSSYNWWENLKIGAGPYPIETSEGWLLIYHGVTLT----CNGYV-- 241 Query: 247 WKKRYTTGIMLLDLHDPGRVIGRXXXXXXXXXXXYETSGGFRNDVIFPGGMILE-DSGEV 305 Y+ G LLDL DP +V+ R YET G F +V+FP + + D+G V Sbjct: 242 ----YSFGAALLDLDDPSKVLYRSRYYLLTPEEEYETVG-FVPNVVFPCAALCDADTGRV 296 Query: 306 KIYYGAADTVECLATAHVDDLL 327 IYYGAADT LA ++D+++ Sbjct: 297 AIYYGAADTHVALAFGYIDEIV 318 >ref|ZP_00059726.1| COG2152: Predicted glycosylase [Clostridium thermocellum ATCC 27405] Length = 334 Score = 162 bits (410), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 112/324 (34%), Positives = 170/324 (52%), Gaps = 33/324 (10%) Query: 14 VITRHPANPILAPGDVPYGPAMVFNAGVTKFKGKYVMVFRNDYGDEAKGIVAPFHTTNLG 73 + R P NPIL+ + PY VFN T + GK +++ R + + +G ++L Sbjct: 24 LFKRFPGNPILSAKNWPYAANTVFNPAATMYNGKVLLLIRVE---DRRGF------SHLT 74 Query: 74 LAFSDDGI-KWEVQPEPCWSWHDE----EVIRVYDPRLTVIG--GQCYMCFAVDTKHGLR 126 A SDDGI W + +P E E V DPR+T I G+ + + +K G Sbjct: 75 KAISDDGISNWIIDDKPTLEAEPEKFPEEEWGVEDPRITWIEELGKFAVVYTAYSKGGPL 134 Query: 127 GGIAVTEDFRSFEVL-SLSLPDNRNMVLFPELIGGKYVRLERPLPVYSRGGIDRFDMWMS 185 +A+TEDF +FE L ++ P++++ LFP I GK+V + RP+ ++ G +W+S Sbjct: 135 VSLALTEDFENFEKLGAIMPPEDKDAALFPRRINGKWVLIHRPISIHHGPGAH---IWIS 191 Query: 186 DSPDLKYWGNSRLLLTVEEVAYAN-DKVGPGAPPVKTDKGWLTLFHAVDLDRSRGKNGWE 244 S DLKYWG+ ++L+ + + + +KVG PP++T GWL L+H V S Sbjct: 192 RSDDLKYWGDHQILIRARKGGWWDANKVGLNCPPLETPDGWLILYHGVRQTAS------- 244 Query: 245 DSWKKRYTTGIMLLDLHDPGRVIGRXXXXXXXXXXXYETSGGFRNDVIFPGGMIL-EDSG 303 Y G+ LLDL +P +V+ R YE G +DV+FP G + E +G Sbjct: 245 ---GSIYRLGLALLDLENPSKVLRRSDEWVFGPQEFYEREGDV-DDVVFPCGWVYNEKTG 300 Query: 304 EVKIYYGAADTVECLATAHVDDLL 327 E+KIYYGAADT +AT ++ DLL Sbjct: 301 EIKIYYGAADTCIAMATTNISDLL 324 >ref|NP_617288.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] gb|AAM05768.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] Length = 335 Score = 155 bits (391), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 105/328 (32%), Positives = 170/328 (51%), Gaps = 33/328 (10%) Query: 14 VITRHPANPILAPGDVPYGPAMVFNAGVTKFKGKYVMVFRNDYGDEAKGIVAPFHTTNLG 73 + RH NPIL D PY VFN + K +++ R ++ +G ++ Sbjct: 9 LFVRHKKNPILTVEDWPYQANSVFNPAAAMVESKTLLLVR---VEDHRGF------SHFT 59 Query: 74 LAFSDDGIK-WEVQPEPCWS----WHDEEVIRVYDPRLTVIG--GQCYMCFAVDTKHGLR 126 +A S++GI WE+ EP + + EE+ + DPR+T I G+ + + + G Sbjct: 60 VARSNNGIDGWEIDTEPTLTPDTVNYPEEIYGIEDPRITYIDEMGRWAVAYTAFSDSGPL 119 Query: 127 GGIAVTEDFRSFEVLSLSLP-DNRNMVLFPELIGGKYVRLERPLPVYSRGGIDRFDMWMS 185 +A+T+DFR+FE + ++P +N++ LFP GK+ L RP+ S + ++W+S Sbjct: 120 TSLALTKDFRNFERIGATMPPENKDSALFPVRFNGKWAMLHRPVSSMSGA---KANIWIS 176 Query: 186 DSPDLKYWGNSRLLLTVEEVAYAN-DKVGPGAPPVKTDKGWLTLFHAVDLDRSRGKNGWE 244 SPD+++WG+ ++LL E + + K+G P++T GWL ++H V S Sbjct: 177 FSPDMRHWGDHQVLLYAREGGWWDARKIGLSPQPIRTYDGWLIMYHGVRPTTS------- 229 Query: 245 DSWKKRYTTGIMLLDLHDPGRVIGRXXXXXXXXXXXYETSGGFRNDVIFPGGMILEDSGE 304 K Y G+ LLDL DP +V+ R YE SG NDV+FP G +L D + Sbjct: 230 ---KLSYRLGMALLDLEDPRKVLHRSEGWVFGPRELYERSGDV-NDVVFPCGWVLHDD-Q 284 Query: 305 VKIYYGAADTVECLATAHVDDLLRLCLE 332 +++YYG+ADT LA+A + D+L E Sbjct: 285 IRLYYGSADTSVSLASAKLHDVLEYIHE 312 >ref|ZP_00076614.1| COG2152: Predicted glycosylase [Methanosarcina barkeri] Length = 324 Score = 154 bits (389), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 104/324 (32%), Positives = 168/324 (51%), Gaps = 33/324 (10%) Query: 14 VITRHPANPILAPGDVPYGPAMVFNAGVTKFKGKYVMVFRNDYGDEAKGIVAPFHTTNLG 73 + R+ NPIL PY + VFN +++ R ++ +G ++L Sbjct: 13 LFVRYKKNPILTADYWPYKVSSVFNPAAVMVDNTTILLVR---AEDHRGF------SHLT 63 Query: 74 LAFSDDGIK-WEVQPEPCWS----WHDEEVIRVYDPRLTVIG--GQCYMCFAVDTKHGLR 126 +A S +GI WE+ P+P ++ ++ EEV + DPR+T I G+ + + + G Sbjct: 64 VARSKNGIDGWEIDPKPTFTSDPTYYSEEVYGIEDPRITYIDEMGKWAIAYTAFSDSGPL 123 Query: 127 GGIAVTEDFRSFE-VLSLSLPDNRNMVLFPELIGGKYVRLERPLPVYSRGGIDRFDMWMS 185 +A TE+F +FE V + P+N++ +FP + G++ L RP+ S + ++W+S Sbjct: 124 TSLAYTENFTNFERVGPIMPPENKDAAIFPTRLNGRWAMLHRPV---SNIAGAKANLWIS 180 Query: 186 DSPDLKYWGNSRLLLTVEEVAYAN-DKVGPGAPPVKTDKGWLTLFHAVDLDRSRGKNGWE 244 SPD+KYWG ++LL E + + K+G PP+KT GWL L++ V S Sbjct: 181 FSPDMKYWGEHKVLLYAREGGWWDARKIGLAPPPMKTSDGWLILYYGVRQTTS------- 233 Query: 245 DSWKKRYTTGIMLLDLHDPGRVIGRXXXXXXXXXXXYETSGGFRNDVIFPGGMILEDSGE 304 KK Y G+ LLDL DP +V+ R YE +G NDV+FP G IL E Sbjct: 234 ---KKSYRLGLALLDLEDPTKVLHRSEGWVFGPREMYERTGDV-NDVVFPCGWILA-GDE 288 Query: 305 VKIYYGAADTVECLATAHVDDLLR 328 ++IYYG+AD +A+A + D+++ Sbjct: 289 LRIYYGSADMSVAMASAKMSDVMQ 312 >ref|NP_634968.1| conserved protein [Methanosarcina mazei Goe1] gb|AAM32640.1| conserved protein [Methanosarcina mazei Goe1] Length = 336 Score = 152 bits (383), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 107/328 (32%), Positives = 166/328 (50%), Gaps = 33/328 (10%) Query: 14 VITRHPANPILAPGDVPYGPAMVFNAGVTKFKGKYVMVFRNDYGDEAKGIVAPFHTTNLG 73 + R+ NPIL D PY VFN GK +++ R + + +G ++ Sbjct: 10 LFVRYKRNPILTVEDWPYQANSVFNPAAVIVDGKTLLLVRVE---DHRGF------SHFT 60 Query: 74 LAFSDDGIK-WEVQPEPCWS----WHDEEVIRVYDPRLTVIG--GQCYMCFAVDTKHGLR 126 +A SD+GI W + PEP ++ + EE+ + DPR+T I G+ + + + G Sbjct: 61 IARSDNGIDGWVIDPEPTFAPDPVNYPEEIYGIEDPRITYIDEIGKWAVAYTAFSDSGPL 120 Query: 127 GGIAVTEDFRSFEVLSLSLP-DNRNMVLFPELIGGKYVRLERPLPVYSRGGIDRFDMWMS 185 +A TEDF++FE + +LP +N++ +FP ++ L RP+ S + ++W+S Sbjct: 121 TALAFTEDFKTFERIGPTLPPENKDAAIFPVKFKDRWAMLHRPV---SNIAGAKANIWIS 177 Query: 186 DSPDLKYWGNSRLLLTVEEVAYAN-DKVGPGAPPVKTDKGWLTLFHAVDLDRSRGKNGWE 244 SPD+KYWG +LL + + + K+G P++ GWL ++H V S Sbjct: 178 FSPDMKYWGEHEVLLYARDGGWWDARKIGLSPQPIRVSDGWLIMYHGVRQTTS------- 230 Query: 245 DSWKKRYTTGIMLLDLHDPGRVIGRXXXXXXXXXXXYETSGGFRNDVIFPGGMILEDSGE 304 K Y G+ LLD DP +V+ R YE SG NDV+FP G +L D E Sbjct: 231 ---KASYRLGMALLDPDDPRKVLHRSEGWIFGPRELYERSGDV-NDVVFPCGWVLFDD-E 285 Query: 305 VKIYYGAADTVECLATAHVDDLLRLCLE 332 ++IYYG+ADT LATA V D+L+ E Sbjct: 286 IRIYYGSADTSVSLATAKVSDVLKYIHE 313 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.142 0.454 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 571,863,593 Number of extensions: 25451024 Number of successful extensions: 39500 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 39384 Number of HSP's successfully gapped: 32 Length of query: 337 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 211 Effective length of database: 415,135,104 Effective search space: 87593506944 Effective search space used: 87593506944 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)