BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0382.0025.p (353 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00163427.1| hypothetical protein Selo196901 [Synechococcu... 165 1e-39 ref|NP_774305.1| blr7665 [Bradyrhizobium japonicum] >gi|27355949... 152 1e-35 gb|AAM70356.1| CalO6 [Micromonospora echinospora] 150 4e-35 ref|ZP_00109917.1| COG0500: SAM-dependent methyltransferases [No... 144 3e-33 pir||JC5855 polyketide synthase (EC 2.-.-.-) chain 6 - Actinomad... 142 1e-32 >ref|ZP_00163427.1| hypothetical protein Selo196901 [Synechococcus elongatus PCC 7942] Length = 331 Score = 165 bits (418), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 107/327 (32%), Positives = 170/327 (51%), Gaps = 6/327 (1%) Query: 24 VVMQIVSGLGFSLAVKTAVELGVFSAFNNGKITVSAMAESLSLNAPALSRLLKALQSISL 83 ++ QI +G S A+ A +LG+ NG + +A + L RLL+AL S+ + Sbjct: 6 LLRQITTGYWGSQALYVAAKLGIADQLVNGPRSCEDLAIVVGAKPEVLYRLLRALASLGI 65 Query: 84 VTQDEEGNYEVTEYGATLLTGKSPRSLEPLVKYLLHETVVQSMVEMKYSIQTGKPAFEKV 143 T+ + + +T A LL + SL LV E V + E+ +SIQTG+ AFE Sbjct: 66 FTEVSDRQFALTP-AAELLRSGTAGSLRNLVMMFGEEHYV-AWGELLHSIQTGENAFEHH 123 Query: 144 FGEQWYGNHEMKDNYLQTMDKAMEIYSRMSLPALLASYPFGQYDVIVDVAGGLGQLISGV 203 +G + + + AM SR A+L+SY F +VDV GG GQL+S + Sbjct: 124 YGAPVFAYYAQHPASADIFNGAMSDMSRPDTAAVLSSYDFQGIRCLVDVGGGHGQLLSQI 183 Query: 204 LQSVPEAKGILFDLPETIERASANIGGSEIGERCTLVPGSMFEQIPSGGSLYMISKVLND 263 L + P+ GIL+D P + A + + +++ +RC +V G F +P+GG Y++ +++D Sbjct: 184 LAAYPDLTGILYDQPAVVAGADSVL--ADVSDRCEVVGGDFFASVPAGGDAYLLKHIIHD 241 Query: 264 WDDEHVVSILANIRKVMTREAKLIIIENLPAKEGLSPEEA-FRDLLFLVCSEGGSVRNLA 322 WDD + ILAN R+VM +L+++E + + G P A + DL LV ++GG R A Sbjct: 242 WDDADSLKILANCRQVMQPGDRLLLLEQV-VRAGNEPNLAKWLDLNMLVMTQGGRERTQA 300 Query: 323 ELGKLVDQAGLDILAVKQTPSKFSIIE 349 E L+ AG + + T S+ IIE Sbjct: 301 EFATLLADAGFQLSQIHGTASEVCIIE 327 >ref|NP_774305.1| blr7665 [Bradyrhizobium japonicum] dbj|BAC52930.1| blr7665 [Bradyrhizobium japonicum USDA 110] Length = 334 Score = 152 bits (385), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 90/318 (28%), Positives = 156/318 (49%), Gaps = 2/318 (0%) Query: 21 PQAVVMQIVSGLGFSLAVKTAVELGVFSAFNNGKITVSAMAESLSLNAPALSRLLKALQS 80 P ++ +++G + A+ A L + + +A + AL RLL+AL + Sbjct: 5 PWLTLLGLINGFQVTQAIHVASTLRIADHLKGDSRSAEELAPLTQSHPGALYRLLRALAA 64 Query: 81 ISLVTQDEEGNYEVTEYGATLLTGKSPRSLEPLVKYLLHETVVQSMVEMKYSIQTGKPAF 140 + + +DE+ + +T G L T S + + + V + + +SI+TG+ AF Sbjct: 65 VGVFEEDEDRRFALTPMGDCLRT-DSATPIGAWAECVGSPYVWSTWGHLLHSIRTGENAF 123 Query: 141 EKVFGEQWYGNHEMKDNYLQTMDKAMEIYSRMSLPALLASYPFGQYDVIVDVAGGLGQLI 200 + + G+ + + D+AM +SR A++ +Y F + +VDV GG G ++ Sbjct: 124 QSLNGKDIWNYRAERPEESAVFDRAMTEFSRGGAEAVIGAYDFSAFHHVVDVGGGRGLML 183 Query: 201 SGVLQSVPEAKGILFDLPETIERASANIGGSEIGERCTLVPGSMFEQIPSGGSLYMISKV 260 + +L + P +G LFD P I A A + + +RC +V GS FE +P GG Y++ V Sbjct: 184 AAILTAHPRMQGTLFDQPGVIAGAEAVLEAHGVVDRCRMVSGSFFESVPEGGDAYVMRVV 243 Query: 261 LNDWDDEHVVSILANIRKVMTREAKLIIIENLPAKEGLSPEEAFRDLLFLVCSEGGSVRN 320 ++DWDD+ ++IL R+ M AKL++IE + A P F DL L GG R Sbjct: 244 IHDWDDDEAIAILKACRRAMRETAKLVLIERIIAPANEVPATKFMDLHMLALP-GGRERT 302 Query: 321 LAELGKLVDQAGLDILAV 338 E L+ ++G ++ V Sbjct: 303 RDEFSDLLAKSGFELTRV 320 >gb|AAM70356.1| CalO6 [Micromonospora echinospora] Length = 356 Score = 150 bits (380), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 105/335 (31%), Positives = 175/335 (52%), Gaps = 16/335 (4%) Query: 27 QIVSGLGFSLAVKTAVELGVFSAFNNGKITVSAMAESLSLNAPALSRLLKALQSISLVTQ 86 Q++ G + + AV L + +G V +A + +AP+L RLL+AL + ++ + Sbjct: 18 QLIYGFFTAQTLHVAVRLRIPDLLADGARDVGDLASATGADAPSLRRLLRALVFLEVLDE 77 Query: 87 DEEGNYEVTEYGATL---LTGKSPRSLEPLVKYLLHETVVQSMVEMKYSIQTGKPAFEKV 143 G + +TE G L +TG S+ LV L + ++++S++TG+ A+E V Sbjct: 78 PAPGTFALTEQGEVLRADVTG----SMRELVLLLSGPESWAAWGQLEHSVRTGEVAWEHV 133 Query: 144 FGEQWYGNHEMKDNYLQT-MDKAMEIYSRMSLPALLASYPFGQYDVIVDVAGGLGQLISG 202 G + +H M D Q + AM SR +P LL++Y FG +VDV GG G L++G Sbjct: 134 HGRSCF-DHLMADPQRQAAFNAAMAEGSRAFVPTLLSAYDFGDLRTVVDVGGGSGALLAG 192 Query: 203 VLQSVPEAKGILFDLPETIERASANIGGSEIGERCTLVPGSMFEQIPSGGSLYMISKVLN 262 VL + P +G +FD P+ + A+ + + +RC + G F +P G Y++ VL+ Sbjct: 193 VLAAHPHLRGTVFDTPDGVADAARTVAEQGVADRCGVETGDFFVSVPPGADAYVLKSVLH 252 Query: 263 DWDDEHVVSILANIRKVMTREAKLIIIENL-PAKEGLSPEEA---FRDLLFLVCSEGGSV 318 DWDDE V +L +R+ + ++++I++E+L P +P A DL +VC GG Sbjct: 253 DWDDEQCVEVLRTVRRAVRPDSRVILVESLMPTTVTTAPSVAQVVMNDLNMMVC-HGGRE 311 Query: 319 RNLAELGKLVDQAGLDILAVKQT--PSKFSIIECA 351 R +AE +L+ AG + +V PS I+E A Sbjct: 312 RTVAEFRELLRVAGFRLESVTPCPAPSVVGILEAA 346 >ref|ZP_00109917.1| COG0500: SAM-dependent methyltransferases [Nostoc punctiforme] Length = 347 Score = 144 bits (363), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 101/339 (29%), Positives = 169/339 (49%), Gaps = 27/339 (7%) Query: 27 QIVSGLGFSLAVKTAVELGVFSAFNNGKITVSAMAESLSLNAPALSRLLKALQSISLVTQ 86 QI++G + + +LG+ +G + +A ++A +L RLL+AL S+ + + Sbjct: 23 QIIAGAWITQGIYVVAQLGIADLLKDGSKSYDELATKTGVDARSLYRLLRALASVGIFAE 82 Query: 87 DEEGNYEVTEYGATLLTGKSPRSLEPLVKYLLHETVV---QSMVEMKYSIQTGKPAFEKV 143 G +E+T +L + ++ + L Y + Q + SI+TGKP F+ + Sbjct: 83 GNSGYFELTPVAESLQSDRT----DSLRGYAIKSGQAWEWQPWGHLLESIKTGKPVFKNI 138 Query: 144 FGEQWYGNHEMKDNYLQTMDKAMEIYSRM-------SLPALLASYPFGQYDVIVDVAGGL 196 FG + + +YL T A +IY++ A+ A Y F +V+V GG Sbjct: 139 FGMERF-------DYLATDPSASKIYTQAISSISGEQDAAIAAGYDFSFIHNLVEVGGGN 191 Query: 197 GQLISGVLQSVPEAKGILFDLPETIERASANIGGSEIGERCTLVPGSMFEQIPSGGSLYM 256 G LI+ +L+S +GILFDLP + A I E+ +RC LV G+ FE +P+ G Y+ Sbjct: 192 GTLIASILKSNLTMQGILFDLPHVVADAKPVIEDLELQDRCQLVGGNFFESVPTSGDAYL 251 Query: 257 ISKVLNDWDDEHVVSILANIRKVMTREAKLIIIENLPAKEGLSPEEAFRDLLFL--VCSE 314 + +++DWDDE ++IL N + M + +L+++E + +G P F LL L + + Sbjct: 252 LRYIIHDWDDERAIAILKNCYQAMQPDGRLLLVE-MVIPQGNEP--FFGKLLDLQMLVNY 308 Query: 315 GGSVRNLAELGKLVDQAGLDILAVKQTPSKFSIIECAVR 353 GG R AE L+ AG + + SIIE A+R Sbjct: 309 GGRERTQAEYQVLLKTAGFSLTKIYPVAPPISIIE-AIR 346 >pir||JC5855 polyketide synthase (EC 2.-.-.-) chain 6 - Actinomadura hibisca dbj|BAA23149.1| ORF 6 [Actinomadura hibisca] Length = 341 Score = 142 bits (357), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 99/344 (28%), Positives = 171/344 (49%), Gaps = 26/344 (7%) Query: 21 PQAVVMQIVS---GLGFSLAVKTAVELGVFSAFNNGKITVSAMAESLSLNAPALSRLLKA 77 P A +++ S G+ + V+ EL V A +G + +A + + AL R+L+ Sbjct: 7 PHAASLRLQSLLDGMRVAKVVQVLAELQVADAVADGPCKPAEIAADVGADPDALYRVLRC 66 Query: 78 LQSISLVTQDEEGNYEVTEYGATLLTGKSPRSLEPLVKYLLHETVVQSMVEMKYSIQTGK 137 S + T+DE+G + +T A L TG S L + + E+ +++TG+ Sbjct: 67 AASFGVFTEDEDGRFGLTPMAALLRTGTD-DSHRDLFMMAAGDLWWRPYGELLETVRTGR 125 Query: 138 PAFEKVFGEQWYGNHEMKDNYLQT-------MDKAMEIYSRMSLPALLASYPFGQYDVIV 190 PA E FG +Y +YL T D+AM S+ A+L F +Y I Sbjct: 126 PAAELAFGMPFY-------DYLGTDPAAAGLFDRAMTQVSKGQAKAILGRCSFERYARIA 178 Query: 191 DVAGGLGQLISGVLQSVPEAKGILFDLPETIERASANIGGSEIGERCTLVPGSMFEQIPS 250 DV GG G ++ VL+S P +G+L DLP + A A + E+ +R +VPGS F+ +P+ Sbjct: 179 DVGGGHGYFLAQVLRSSPRTEGVLLDLPHVVAGAPAVLEKHEVADRVQVVPGSFFDALPT 238 Query: 251 GGSLYMISKVLNDWDDEHVVSILANIRKVM--TREAKLIIIEN-LPAKEGLSPEEAFR-D 306 G Y++ +L +W D IL +R+ + R+A+L+++E +P + +A D Sbjct: 239 GCDAYLLKAILINWPDADAERILHRVREAIGTDRDARLLVVEPVVPPGDVRDYSKATDID 298 Query: 307 LLFLVCSEGGSVRNLAELGKLVDQAGLDILAVKQTPSKFSIIEC 350 +L ++ GG R +AE +L+ G +++ + TP + ++EC Sbjct: 299 MLAII---GGRQRTVAEWRRLLRAGGFELVG-EPTPGRREVMEC 338 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.133 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 478,881,371 Number of extensions: 18921794 Number of successful extensions: 60236 Number of sequences better than 1.0e-05: 268 Number of HSP's gapped: 59873 Number of HSP's successfully gapped: 268 Length of query: 353 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 226 Effective length of database: 412,420,005 Effective search space: 93206921130 Effective search space used: 93206921130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)