BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0382.0022.p (837 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAB06816.1| amlC [Streptomyces lividans] 601 e-170 ref|NP_631083.1| secreted alpha-amylase. [Streptomyces coelicolo... 600 e-170 ref|ZP_00178179.1| hypothetical protein Cwat143301 [Crocosphaera... 198 4e-49 prf||2020344A alpha amylase 148 5e-34 sp|P21543|AMYB_PAEPO Beta/alpha-amylase precursor [Includes: Bet... 135 3e-30 >emb|CAB06816.1| amlC [Streptomyces lividans] Length = 993 Score = 601 bits (1550), Expect = e-170, Method: Compositional matrix adjust. Identities = 325/645 (50%), Positives = 404/645 (62%), Gaps = 57/645 (8%) Query: 240 DFREDSIYFVITSRFYDGDPSNNEHAWDDAKANNP-DSDPAWRGDFKGLIQKLDYIKALG 298 D R+D IYFV+T+RF DGD +NN K+ N + DP +RGDFKGL+ KLDYIK LG Sbjct: 359 DPRKDPIYFVLTARFNDGDATNNRGGSQHEKSGNAANDDPMFRGDFKGLVNKLDYIKGLG 418 Query: 299 FSAIWITPVVQNASGYDYHGYHAINFAKVDPRYESAGASYQDLINAAHAKGIKIIQDVVV 358 FSA+WITPVV N S YDYHGYH +F KVDPR ESAGASYQDLI+AAHAKG+KI QDVV Sbjct: 419 FSAVWITPVVLNRSDYDYHGYHGYDFYKVDPRLESAGASYQDLIDAAHAKGMKIYQDVVY 478 Query: 359 NHTGNFGEENLFP-----------MFKKDETKPDTVSNLLKITDKLPSNY---------- 397 NH+ +G LF + DE +P + L + K +Y Sbjct: 479 NHSSRWGARGLFTPTVYGVRDSQWSWYYDEKQPGFEYDGLTVEPKSGKSYYNGDLWSTAE 538 Query: 398 -------------DSMTPD--QQYQARIALMKTAETNNNMYHTEKSLSWESYTVQTGQIA 442 + +P+ Y + + +YH +WE ++ + Sbjct: 539 PTGNTCVNWGRPTGAKSPEGYTLYDCQWPSPTSGMFPKALYHNCWIGNWEGEDSRSCWLH 598 Query: 443 GDCVDLNTENPVVDQYLIDSYNQYIDMGVDAFRLDTVKHVSRYIFNKYFIPAWKTR---- 498 D D NTENP V YLI +Y++YIDMGVD FR+DT H+ R +N+ F+PA + R Sbjct: 599 EDLADFNTENPQVQNYLIGAYDKYIDMGVDGFRVDTAVHIPRTTWNRRFLPAIQERVAQQ 658 Query: 499 ----GGSDFFMFGEVATRYRDVWNSGIPAISTPFYTWKSAKSYPGDSTNDYASNKASVEQ 554 +FF+FGEVA D WN G S FYTWK K Y D KA++E Sbjct: 659 HGAEAAKNFFVFGEVAAFVNDKWNRGSVTHSAQFYTWKERKEYSADDA------KAALEM 712 Query: 555 -EWADNSTTAGQPTSNNAFLNGNTYHAPDYSMKSGMDVIDFPMHWAFKTAQEAF-SMRSG 612 E+ T QPTS NAFL GN+YH PD+S SGM+VID MH F A AF + + Sbjct: 713 YEYEQQQGTGNQPTSANAFLEGNSYHVPDHSRFSGMNVIDMRMHMNFGDASNAFHNGKDS 772 Query: 613 DQFYNDATWNVTYVDSHDYAPDQAPENQRFAGTQDTWAENLALMFTFRGIPAIYYGSEIE 672 D YNDAT+NV YVDSHDY P+++ E RF+G D WAEN++LM+TFRGIP +YYGSEIE Sbjct: 773 DDSYNDATYNVVYVDSHDYGPNKSSE--RFSGGTDAWAENMSLMWTFRGIPTLYYGSEIE 830 Query: 673 FKKGVVLDVGPNAPLSATGRAYYGDHIEGSVTVQDYGKYTNATGTLAESLNYPLAKHIRQ 732 F+KG +D GP PL+ TGRAYYGDH+ G VT D+ + +ATG +A +L PL KH+++ Sbjct: 831 FQKGRKIDCGPTCPLATTGRAYYGDHLAGDVTASDFSRVDSATGAVATTLAQPLVKHVQR 890 Query: 733 LNLIRRAVPALQKGQYSTENVSGNLAFKRRYTDSAKGIDSFALVTISGNATFTGIPNGTY 792 LN IRRAVPALQ GQYST+ V+G++AFKRRYTD G DSFALVT+SG A + GIPNGTY Sbjct: 891 LNQIRRAVPALQMGQYSTDGVTGDMAFKRRYTDG--GTDSFALVTVSGGAAYDGIPNGTY 948 Query: 793 VDAVTGDTKTVTNGTITLTCSGKGNARVYVLNGKGGIGETGPYLK 837 DAVTGDT+TVT+G +T+ GKGN RVYVLNG G IG GPYLK Sbjct: 949 RDAVTGDTRTVTDGRLTVAAPGKGNLRVYVLNGPGKIGADGPYLK 993 >ref|NP_631083.1| secreted alpha-amylase. [Streptomyces coelicolor A3(2)] emb|CAB88152.1| secreted alpha-amylase. [Streptomyces coelicolor A3(2)] Length = 993 Score = 600 bits (1547), Expect = e-170, Method: Compositional matrix adjust. Identities = 324/645 (50%), Positives = 405/645 (62%), Gaps = 57/645 (8%) Query: 240 DFREDSIYFVITSRFYDGDPSNNEHAWDDAKANNP-DSDPAWRGDFKGLIQKLDYIKALG 298 D R+D IYFV+T+RF DGD +NN K+ N + DP +RGDFKGL+ KLDYIK LG Sbjct: 359 DPRKDPIYFVLTARFNDGDATNNRGGSQHEKSGNAANDDPMFRGDFKGLVNKLDYIKGLG 418 Query: 299 FSAIWITPVVQNASGYDYHGYHAINFAKVDPRYESAGASYQDLINAAHAKGIKIIQDVVV 358 FSA+WITPVV N S YDYHGYH +F KVDPR ESAGASYQDLI+AAHAKG+KI QDVV Sbjct: 419 FSAVWITPVVLNRSDYDYHGYHGYDFYKVDPRLESAGASYQDLIDAAHAKGMKIYQDVVY 478 Query: 359 NHTGNFGEENLFP-----------MFKKDETKPDTVSNLLKITDKLPSNY---------- 397 NH+ +G + LF + DE +P + L + K +Y Sbjct: 479 NHSSRWGAKGLFTPTVYGVRDSQWSWYYDEKQPGFEYDGLTVEPKSGKSYYNGDLWSTAE 538 Query: 398 -------------DSMTPD--QQYQARIALMKTAETNNNMYHTEKSLSWESYTVQTGQIA 442 + +P+ Y + + +YH +WE ++ + Sbjct: 539 PTGNTCVNWGRPTGAKSPEGYTLYDCQWPSPTSGMFPKALYHNCWIGNWEGEDSRSCWLH 598 Query: 443 GDCVDLNTENPVVDQYLIDSYNQYIDMGVDAFRLDTVKHVSRYIFNKYFIPAWKTR---- 498 D D NTENP V YLI +Y++YIDMGVD FR+DT H+ R +N+ F+PA + R Sbjct: 599 EDLADFNTENPQVQNYLIGAYDKYIDMGVDGFRVDTAVHIPRTTWNRRFLPAIQERVAQQ 658 Query: 499 ----GGSDFFMFGEVATRYRDVWNSGIPAISTPFYTWKSAKSYPGDSTNDYASNKASVEQ 554 +FF+FGEVA D WN G S FYTWK K Y D KA++E Sbjct: 659 HGAEAAKNFFVFGEVAAFVNDKWNRGSVNHSAQFYTWKERKEYSADDA------KAALEM 712 Query: 555 -EWADNSTTAGQPTSNNAFLNGNTYHAPDYSMKSGMDVIDFPMHWAFKTAQEAF-SMRSG 612 E+ T QPTS NAFL GN+YH PD+S SGM+VID MH F A AF + + Sbjct: 713 YEYEQQQGTGNQPTSANAFLEGNSYHVPDHSRFSGMNVIDMRMHMNFGDASNAFHNGKDS 772 Query: 613 DQFYNDATWNVTYVDSHDYAPDQAPENQRFAGTQDTWAENLALMFTFRGIPAIYYGSEIE 672 D YNDAT+NV YVDSHDY P+++ E RF+G D WAEN++LM+TFRGIP +YYGSEIE Sbjct: 773 DDSYNDATYNVVYVDSHDYGPNKSSE--RFSGGTDAWAENMSLMWTFRGIPTLYYGSEIE 830 Query: 673 FKKGVVLDVGPNAPLSATGRAYYGDHIEGSVTVQDYGKYTNATGTLAESLNYPLAKHIRQ 732 F+KG +D GP PL+ TGRAYYGDH+ G VT D+ + +ATG +A +L PL KH+++ Sbjct: 831 FQKGRKIDCGPTCPLATTGRAYYGDHLAGDVTASDFSRVDSATGAVATTLAQPLVKHVQR 890 Query: 733 LNLIRRAVPALQKGQYSTENVSGNLAFKRRYTDSAKGIDSFALVTISGNATFTGIPNGTY 792 LN IRRAVPALQ GQYST+ ++G++AFKRRYTD G DSFALVT+SG A + GIPNGTY Sbjct: 891 LNQIRRAVPALQMGQYSTDGITGDMAFKRRYTDG--GTDSFALVTVSGGAAYDGIPNGTY 948 Query: 793 VDAVTGDTKTVTNGTITLTCSGKGNARVYVLNGKGGIGETGPYLK 837 DAVTGDT+TVT+G +T+ GKGN RVYVLNG G IG GPYLK Sbjct: 949 RDAVTGDTRTVTDGRLTVAAPGKGNLRVYVLNGPGKIGADGPYLK 993 >ref|ZP_00178179.1| hypothetical protein Cwat143301 [Crocosphaera watsonii WH 8501] Length = 406 Score = 198 bits (504), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 152/435 (34%), Positives = 218/435 (50%), Gaps = 82/435 (18%) Query: 421 NMYHTEKSLS---WESYTVQTGQIAGDCVDLNTENPVVDQYLIDSYNQYIDMGVDAFRLD 477 N YH + +S WES +QT +AGD +DL T V YLI++ QY+DMGVDA R+D Sbjct: 34 NWYHQDGFMSGGDWESVAIQTKHLAGDTIDLATRRDNVKDYLINAICQYLDMGVDALRID 93 Query: 478 TVKHVSRYIFNKYFIPAWKTRGGSDFFMFGE---VATRYRDVW-NSGIPAISTPFYTWKS 533 TVKHV R +Y I AWK S F+FGE T + D++ + P+ P++ Sbjct: 94 TVKHVERNNLLEY-IDAWKAHKPS-LFVFGENLVKGTGWGDLFGDDNAPSFLRPWWY--- 148 Query: 534 AKSYPGDSTNDYASNKASVEQEWADNSTTAGQPTSNNAFLNGNTYHAPDYSMKSGMDVID 593 + GD D P SG V+D Sbjct: 149 --TRLGDDPKD------------------------------------PHSGGDSGFSVLD 170 Query: 594 FPMHWAFK--TAQEAF----SMRSGDQFYNDATWNVTYVDSHDYAPDQAPENQRFAGTQD 647 F + F+ ++ +F ++ + D Y DAT VT++ +HD PD + RF G + Sbjct: 171 FSLFSTFRDNLSRGSFNGIGAILANDWVYGDATTLVTFLQNHDVGPDNDFRD-RFRGNTE 229 Query: 648 TWAENLALMFTFRGIPAIYYGSEIEFKKGVVLDV-GPNAPLSATGRAYYGDHIEGSVTVQ 706 A L++T RGIP +YYG EIEF KG D+ G + L TGRAY+GDH+E Sbjct: 230 WAAAAYNLLWTIRGIPCLYYGEEIEFMKGKPQDIMGNDDTLDQTGRAYFGDHLEDD---- 285 Query: 707 DYGKYTNATGTLAESLNYPLAKHIRQLNLIRRAVPALQKGQYSTENVSGN-LAFKRRYTD 765 T+ ++ ++PL +HI++LN+IR +PALQK S N G+ ++F R Sbjct: 286 ----------TIQKTQSHPLYQHIKRLNIIRSRIPALQKAPMSQVNEWGSGMSFVRE--- 332 Query: 766 SAKGIDSFALV--TISGNA--TFTGIPNGTYVDAVTGDTKTVTNGTITLTCSGKGNARVY 821 +G +S+ +V TI G T + NGTY DAVTG++ V + +I+ +A +Y Sbjct: 333 -DQGSNSYVVVGLTIGGQQQITINDVHNGTYRDAVTGNSIDVNSNSISFDVKAN-SAGIY 390 Query: 822 VLNGKGGIGETGPYL 836 VLNG+G IGE G YL Sbjct: 391 VLNGEGKIGEDGVYL 405 >prf||2020344A alpha amylase Length = 453 Score = 148 bits (374), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 165/589 (28%), Positives = 235/589 (39%), Gaps = 160/589 (27%) Query: 240 DFREDSIYFVITSRFYDGDPSNNEHAWDDAKANNPDSDPAWRGDFKGLIQKLDYIKALGF 299 D++E SIYF++T RF +GDPSN+ + +A ++P GDF+G+I +LDYIK +GF Sbjct: 5 DWQEKSIYFIMTDRFDNGDPSNDNYGGFNADKSDPRKYHG--GDFQGIINRLDYIKNMGF 62 Query: 300 SAIWITPVVQNASGYDYHGYHAINFAKVDPRYESAGASYQDLINAAHAKGIKIIQDVVVN 359 +AIWITPV S YHGY +F VD S A Q+L+ AAHAKGI ++ DVV N Sbjct: 63 TAIWITPVTMQKSANAYHGYWTYDFYSVDGHLGSM-AKLQELVRAAHAKGISVMLDVVAN 121 Query: 360 HTGNFGEENLFPMFKKDETKPDTVSNLLKITDKLPSNYDSMTPDQQYQARIALMKTAETN 419 HTG+F PS++ D+ Sbjct: 122 HTGDF----------------------------QPSSFAKAPFDKA-------------- 139 Query: 420 NNMYHTEKSLSW-ESYTVQTGQIAGDCVDLNTENPVVDQYLIDSYNQYID-MGVDAFRLD 477 + +H + W + + V+ G IAG DLN +NP V L + + GVD R+D Sbjct: 140 DWYHHYGDTQDWNDQWWVENGDIAG-LDDLNQDNPAVATELKNWIAWLVQTTGVDGLRVD 198 Query: 478 TVKHVSRYIFNKYFIPAWKTRGGSDFFMFGEVATRYRDVWNSGIPAISTPFYTWKSAKSY 537 TVKHV ++ + ++ G ++ F GEV G PA Y Sbjct: 199 TVKHVPKWFWREF-------DGAANTFTLGEVF--------HGDPA-------------Y 230 Query: 538 PGDSTNDYASNKASVEQEWADNSTTAGQPTSNNAFLNGNTYHAPDYSMKSGMDVIDFPMH 597 GD TN Y + T N F D SM+ D Sbjct: 231 VGDYTN-YLDAVLDFPMYY----------TIKNVF-------GQDQSMRQIAD------- 265 Query: 598 WAFKTAQEAFSMRSGDQFYNDATWNVTYVDSHDYAPDQAPENQRFAGTQDTWAE---NLA 654 + AQ D Y + N ++D+HD + + + D W + L Sbjct: 266 ---RYAQ--------DYRYKNPMTNGLFIDNHDVPRFLNEASGKPGASWDKWPQLKAALG 314 Query: 655 LMFTFRGIPAIYYGSEIEFKKGVVLDVGPNAPLSATGRAYYGDHIEGSVTVQDYGKYTNA 714 MFT RGIP +Y G+E F G P + + H Sbjct: 315 FMFTTRGIPILYQGTEQGFSGGA-------DPYNRDDMVFNDKH---------------- 351 Query: 715 TGTLAESLNYPLAKHIRQLNLIRRAVPALQKGQYSTENVSGNL-AFKRRYTDSAKGIDSF 773 L +I +LN IR AL+KG + + V AF+R Y G + Sbjct: 352 ----------ELYGYIAKLNYIRNTHVALRKGTQAEKWVDDTFYAFQRSYN----GDEVV 397 Query: 774 ALVTISGNATFTGIPN-------GTYVDAVTGDTKTVTNGTITLTCSGK 815 ++ S N+ +PN T + + D TV NG+IT T K Sbjct: 398 VMINNSWNSQTRTVPNIDRVANGQTLYNRMGTDKVTVNNGSITATLGPK 446 >sp|P21543|AMYB_PAEPO Beta/alpha-amylase precursor [Includes: Beta-amylase ; Alpha-amylase ] pir||A29130 beta-amylase (EC 3.2.1.2) / alpha-amylase (EC 3.2.1.1) precursor - Bacillus polymyxa gb|AAA85446.1| beta-amylase Length = 1196 Score = 135 bits (341), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 93/246 (37%), Positives = 124/246 (50%), Gaps = 41/246 (16%) Query: 240 DFREDSIYFVITSRFYDGDPSNNEHAWDDAKANNPDSDPAWRGDFKGLIQKLDYIKALGF 299 D+ + SIYF++T RF +GDPSN+ + +NN D GDF+G+I KLDYIK +GF Sbjct: 743 DWSKQSIYFIMTDRFSNGDPSNDNYG--GFNSNNSDQRKWHGGDFQGIINKLDYIKNMGF 800 Query: 300 SAIWITPVVQNASGYDYHGYHAINFAKVDPRYESAGASYQDLINAAHAKGIKIIQDVVVN 359 +AIWITPV S Y YHGYH +F VD + Q+L+ AH K I ++ DVVVN Sbjct: 801 TAIWITPVTMQKSEYAYHGYHTYDFYAVDGHLGTM-DKLQELVRKAHDKNIAVMVDVVVN 859 Query: 360 HTGNFGEENLFPMFKKDETKPDTVSNLLKITDKLPSNYDSMTPDQQYQARIALMKTAETN 419 HTG+F N F D K D + ITD +Y+S Sbjct: 860 HTGDFQPGNGFAKAPFD--KADWYHHNGDITD---GDYNS-------------------- 894 Query: 420 NNMYHTEKSLSWESYTVQTGQIAGDCVDLNTENPVVDQYLIDSYNQYI-DMGVDAFRLDT 478 NN + ++ G +AG DLN ENP L + + + G+D RLDT Sbjct: 895 NNQWK-----------IENGDVAG-LDDLNHENPATANELKNWIKWLLNETGIDGLRLDT 942 Query: 479 VKHVSR 484 VKHV + Sbjct: 943 VKHVPK 948 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.130 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,358,866,867 Number of extensions: 61074445 Number of successful extensions: 121287 Number of sequences better than 1.0e-05: 669 Number of HSP's gapped: 120611 Number of HSP's successfully gapped: 683 Length of query: 837 Length of database: 757,237,578 Length adjustment: 134 Effective length of query: 703 Effective length of database: 393,414,312 Effective search space: 276570261336 Effective search space used: 276570261336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)