BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0014.p (363 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAK79341.1| unknown [environmental sequence] 226 8e-58 ref|NP_635076.1| oxidoreductase [Methanosarcina mazei Goe1] >gi|... 216 7e-55 gb|AAN04236.1| putative bi-domain oxidoreductase [Streptomyces s... 216 9e-55 ref|NP_624709.1| putative bi-domain oxidoreductase [Streptomyces... 209 6e-53 ref|NP_633204.1| oxidoreductase [Methanosarcina mazei Goe1] >gi|... 202 9e-51 >gb|EAK79341.1| unknown [environmental sequence] Length = 301 Score = 226 bits (575), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 109/270 (40%), Positives = 169/270 (62%), Gaps = 4/270 (1%) Query: 60 KPVVLGYSAAGVIRSRGKGMEHWPEGQRVACYGAPYAKHAEWLLMPQHLMVPVPEHVSPE 119 P LGYS+AG + GK + + G RVAC G+ YA H+E++ +P++L +PE+++ + Sbjct: 6 NPTPLGYSSAGTVTETGKDVHQFSPGDRVACIGSGYASHSEFISVPENLCCKIPENLTMQ 65 Query: 120 EASTVGLGAIAVHAVRQADLQFGETLVLIGAGILGQLIAQIARAAGCRVIVYDLLAERCR 179 +A+ +GAIA++ +R A+L FGET+V+IG G+LGQL QI +A GC+VI D+ ++ Sbjct: 66 DAAFGMVGAIALNGIRCANLTFGETVVVIGLGLLGQLTTQILKAYGCKVIAMDINPKKLG 125 Query: 180 TAESLGIRHIAVNPQRVSRHLDNLTAGMGADAVIICAGGKSGGLVDQGLEWVRNQGKVLL 239 + + + + +T G GADA II S G +D LE ++ +G ++L Sbjct: 126 EKNNFTADFSFDSESALIEGTEKITKGYGADAAIITTASSSSGPIDTSLEVIKFKGTIVL 185 Query: 240 VG--DVKPDFSRELMFGKEAQVLISRAGGPGRYDPVYEKQGIDYPYGYVRWTEGRNMAEY 297 VG D+ P+ R M+ KEAQ+++S+A GPG +DP+YE +G+DYP +VRWTE RN+ E Sbjct: 186 VGTADIHPE--RNDMWHKEAQIIVSKAAGPGTFDPIYENKGVDYPLSHVRWTENRNLEEL 243 Query: 298 IRLISEGDIRVKPLISSVFPLERSADAFRQ 327 I LIS+ I K LIS F +E + D ++ Sbjct: 244 IHLISKEKINTKNLISHKFKIEEAEDVYQN 273 >ref|NP_635076.1| oxidoreductase [Methanosarcina mazei Goe1] gb|AAM32748.1| oxidoreductase [Methanosarcina mazei Goe1] Length = 720 Score = 216 bits (550), Expect = 7e-55, Method: Compositional matrix adjust. Identities = 134/372 (36%), Positives = 199/372 (53%), Gaps = 41/372 (11%) Query: 11 MKAVVS--QNGQISVADIPAPVLEDGFVLVETEYSAISPGTE------------------ 50 MK VV ++G ++V ++P P ++ VLV YS IS GTE Sbjct: 1 MKQVVLNLKDGDLTVEEVPIPTIKGSGVLVRNHYSVISAGTESGVVDLADKSLIGKARAR 60 Query: 51 --IMMNGIHRPK---PVV--------------LGYSAAGVIRSRGKGMEHWPEGQRVACY 91 + M I++ K P+ LGYS+AG + S + ++ G RVAC Sbjct: 61 PDLAMKVINKAKQDGPISAFQQAMGRLEKREPLGYSSAGTVISVSEDIKDIKPGDRVACA 120 Query: 92 GAPYAKHAEWLLMPQHLMVPVPEHVSPEEASTVGLGAIAVHAVRQADLQFGETLVLIGAG 151 GA YA HA+ + +P++L VPE+V + A +G+IA+ VR AD++ GE +V+IG G Sbjct: 121 GAGYANHADVVFVPRNLCAKVPENVDFKNACFTTVGSIAMQGVRNADVKIGENIVVIGLG 180 Query: 152 ILGQLIAQIARAAGCRVIVYDLLAERCRTAESLGIR-HIAVNPQRVSRHLDNLTAGMGAD 210 ++G + QI +A+GCRV DL + A LG + N + + + G+GAD Sbjct: 181 LIGLITLQILKASGCRVFGVDLDESKVNLARELGADLSYSRNSPNLEERIKQFSRGIGAD 240 Query: 211 AVIICAGGKSGGLVDQGLEWVRNQGKVLLVGDVKPDFSRELMFGKEAQVLISRAGGPGRY 270 + II A +S VD + R +GKV++VG V D RE + KE ++ ISR+ GPGRY Sbjct: 241 STIITAATRSNDPVDFAGKITRERGKVVIVGLVGMDIPREEYYNKELEMRISRSYGPGRY 300 Query: 271 DPVYEKQGIDYPYGYVRWTEGRNMAEYIRLISEGDIRVKPLISSVFPLERSADAFRQYAE 330 D YE+ G DYP YVRWTE RNM ++ LIS I ++P+I+ + +E++ +A+ E Sbjct: 301 DRNYEEYGQDYPAPYVRWTENRNMQAFLDLISMKKISMEPIITHEYEIEQAPEAYNIIKE 360 Query: 331 SPAELLGAVLAY 342 LG VL Y Sbjct: 361 RKP-YLGLVLKY 371 >gb|AAN04236.1| putative bi-domain oxidoreductase [Streptomyces sp. 139] Length = 730 Score = 216 bits (549), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 135/373 (36%), Positives = 196/373 (52%), Gaps = 42/373 (11%) Query: 11 MKAVVS--QNGQISVADIPAPVLEDGFVLVETEYSAISPGTEIM--------MNGIHRPK 60 MK VV ++G++++ D+P P + G VLV T +S IS GTE+M M G R + Sbjct: 1 MKQVVQNYKSGELALLDVPEPGCKPGGVLVRTAFSLISTGTELMKVSEAGMSMLGKARSR 60 Query: 61 P-----------------------------VVLGYSAAGVIRSRGKGMEHWPEGQRVACY 91 P LGYS GV+ G G++ G VAC Sbjct: 61 PDQVAKVVQSVAANGVPATYRKVMGKLDSYTPLGYSLCGVVEQVGAGIDDVKAGDLVACA 120 Query: 92 GAPYAKHAEWLLMPQHLMVPVPEHVSPEEASTVGLGAIAVHAVRQADLQFGETLVLIGAG 151 G +A HAE +P++L VPVP+ V+P A+ +G+IA+ VRQ + + GE ++IG G Sbjct: 121 GNEHALHAELNWVPKNLYVPVPDGVAPRHAAFGTVGSIALQGVRQGESRLGEVALVIGLG 180 Query: 152 ILGQLIAQIARAAGCRVIVYDLLAERCRTAESLGIRHIAVNP--QRVSRHLDNLTAGMGA 209 ++GQL+ Q+ A+G RV+ D RC AE LG +P V + LT GMG Sbjct: 181 LIGQLVVQLLNASGVRVVGVDPDPARCELAERLGAAACG-DPGSASVESAVAELTGGMGV 239 Query: 210 DAVIICAGGKSGGLVDQGLEWVRNQGKVLLVGDVKPDFSRELMFGKEAQVLISRAGGPGR 269 D V + AGG S V+ R++G+V+ +G + D + KE V SR+ GPGR Sbjct: 240 DQVYLAAGGSSNQPVELAARLCRDRGRVVDIGKCRLDLPWNAYYEKELDVRFSRSYGPGR 299 Query: 270 YDPVYEKQGIDYPYGYVRWTEGRNMAEYIRLISEGDIRVKPLISSVFPLERSADAFRQYA 329 YDP YE QG DYP GYVRWTE RN+A ++ LI+ G + V+PL+S V E + + +R+ Sbjct: 300 YDPEYELQGRDYPIGYVRWTERRNLACFLDLIARGRVDVEPLVSHVSGFEDAVETYRRLE 359 Query: 330 ESPAELLGAVLAY 342 + + + + Y Sbjct: 360 DGELKAVAVLFRY 372 >ref|NP_624709.1| putative bi-domain oxidoreductase [Streptomyces coelicolor A3(2)] emb|CAB58328.1| putative bi-domain oxidoreductase [Streptomyces coelicolor A3(2)] Length = 726 Score = 209 bits (533), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 128/366 (34%), Positives = 192/366 (52%), Gaps = 40/366 (10%) Query: 17 QNGQISVADIPAPVLEDGFVLVETEYSAISPGTEIM--------MNGIHRPKP------- 61 ++G++++ D+P P + G VLV T YS IS GTE+M M G R +P Sbjct: 5 KSGELALLDVPVPGCKPGGVLVRTAYSLISTGTELMKVSEAGMSMLGKARSRPDQVAKVV 64 Query: 62 ----------------------VVLGYSAAGVIRSRGKGMEHWPEGQRVACYGAPYAKHA 99 LGYS GV+ G+G++ G VAC G +A HA Sbjct: 65 QSVATNGVPATYRKVMGKLDSYTPLGYSLCGVVEQVGEGIDDVKAGDLVACAGNEHALHA 124 Query: 100 EWLLMPQHLMVPVPEHVSPEEASTVGLGAIAVHAVRQADLQFGETLVLIGAGILGQLIAQ 159 E +P++L PVP+ ++ +A+ +G+IA+ VRQ + + GET ++IG G++GQL+ Q Sbjct: 125 ELNWVPRNLYAPVPDGLAARDAAFGTVGSIALQGVRQGESRLGETALVIGLGLIGQLVVQ 184 Query: 160 IARAAGCRVIVYDLLAERCRTAESLGIRHIAVNPQR--VSRHLDNLTAGMGADAVIICAG 217 + A+G RV+ D AERC AE LG +P V + LT G G D V + AG Sbjct: 185 LLVASGVRVVGADPDAERCELAERLGAAACG-DPASPAVESAVVELTDGHGVDQVYLAAG 243 Query: 218 GKSGGLVDQGLEWVRNQGKVLLVGDVKPDFSRELMFGKEAQVLISRAGGPGRYDPVYEKQ 277 G S V+ R++G+V+ +G + D + KE V SR+ GPGRYDP YE + Sbjct: 244 GGSNQPVELAARLCRDRGRVVDIGKCRLDLPWNAYYEKELDVRFSRSYGPGRYDPEYELE 303 Query: 278 GIDYPYGYVRWTEGRNMAEYIRLISEGDIRVKPLISSVFPLERSADAFRQYAESPAELLG 337 G DYP GYVRWTE RN+A ++ L++ G + V PLI+ + + + +R + + + Sbjct: 304 GRDYPIGYVRWTERRNLACFLDLVARGRVDVAPLITRTADFDDAVETYRSLQDGALKAVA 363 Query: 338 AVLAYP 343 + YP Sbjct: 364 VLFRYP 369 >ref|NP_633204.1| oxidoreductase [Methanosarcina mazei Goe1] gb|AAM30876.1| oxidoreductase [Methanosarcina mazei Goe1] Length = 724 Score = 202 bits (514), Expect = 9e-51, Method: Compositional matrix adjust. Identities = 136/373 (36%), Positives = 200/373 (53%), Gaps = 41/373 (10%) Query: 10 NMKAVVS--QNGQISVADIPAPVLEDGFVLVETEYSAISPGTE----------------- 50 +MK VV ++G ++V D+P P L+ VLV YS IS GTE Sbjct: 6 SMKQVVLNLKDGDLTVEDVPIPTLKGSGVLVRNYYSVISAGTESGLVDLADKSLIGKARA 65 Query: 51 ---IMMNGIHRPK---PVV--------------LGYSAAGVIRSRGKGMEHWPEGQRVAC 90 + M I++ K P+ LGYS+AG + S + + G RVAC Sbjct: 66 RPDLAMKVINKAKQDGPISAFQQAMGRLEKREPLGYSSAGTVISVSEDIASIKVGDRVAC 125 Query: 91 YGAPYAKHAEWLLMPQHLMVPVPEHVSPEEASTVGLGAIAVHAVRQADLQFGETLVLIGA 150 GA YA HA+ +P++L V VP++V + A +G+IA+ VR AD++ GE +V++G Sbjct: 126 AGAGYANHADVAFVPKNLCVKVPDNVDLKNACFTTVGSIAMQGVRNADVRVGENVVVVGL 185 Query: 151 GILGQLIAQIARAAGCRVIVYDLLAERCRTAESLGIRH-IAVNPQRVSRHLDNLTAGMGA 209 G++G + QI +A+GCRV DL + + A+ LG ++ N + + + G+GA Sbjct: 186 GLIGLITVQILKASGCRVFGIDLDESKVKLAKELGADFALSRNTPNIEESVRQFSRGIGA 245 Query: 210 DAVIICAGGKSGGLVDQGLEWVRNQGKVLLVGDVKPDFSRELMFGKEAQVLISRAGGPGR 269 D II A +S VD + R +GKV++VG V D RE + KE ++ +SR+ GPGR Sbjct: 246 DVTIIAAATRSNDPVDFAGKITRERGKVVIVGLVGMDIPREEYYNKEIELRVSRSYGPGR 305 Query: 270 YDPVYEKQGIDYPYGYVRWTEGRNMAEYIRLISEGDIRVKPLISSVFPLERSADAFRQYA 329 YD YE+ G DYP YVRWTE RNM ++ L+S I + P+IS F +E++ DA+ Sbjct: 306 YDRAYEEFGRDYPAPYVRWTENRNMQAFLDLLSMKKISMDPIISHEFAIEQAPDAY-DII 364 Query: 330 ESPAELLGAVLAY 342 E LG VL Y Sbjct: 365 EQRKPYLGIVLRY 377 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 533,427,489 Number of extensions: 23457002 Number of successful extensions: 63722 Number of sequences better than 1.0e-05: 744 Number of HSP's gapped: 63871 Number of HSP's successfully gapped: 744 Length of query: 363 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 236 Effective length of database: 412,420,005 Effective search space: 97331121180 Effective search space used: 97331121180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)