BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0001.p (529 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|P31835|CDG2_PAEMA Cyclomaltodextrin glucanotransferase precur... 818 0.0 gb|AAC04359.1| alpha-cyclodextrin glucanotransferase [Paenibacil... 789 0.0 sp|P04830|CDG1_PAEMA Cyclomaltodextrin glucanotransferase precur... 787 0.0 gb|AAA22298.1| cyclodextrin glucanotransferase 741 0.0 sp|P26827|CDGT_THETU Cyclomaltodextrin glucanotransferase precur... 705 0.0 >sp|P31835|CDG2_PAEMA Cyclomaltodextrin glucanotransferase precursor (Cyclodextrin-glycosyltransferase) (CGTase) pir||ALBSXR cyclomaltodextrin glucanotransferase (EC 2.4.1.19) precursor [validated] - Bacillus macerans Length = 713 Score = 818 bits (2114), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/470 (81%), Positives = 431/470 (91%) Query: 1 MVLGAAVPALADPATSVNNKVNYSTDVIYQIVTDRFVDGNPANNPTGAAFSSDHSNMKLY 60 + LGAA+P A P TSVNNK+N+STD +YQIVTDRFVDGN ANNPTGAAFSSDHSN+KLY Sbjct: 17 IALGAALPVWASPDTSVNNKLNFSTDTVYQIVTDRFVDGNSANNPTGAAFSSDHSNLKLY 76 Query: 61 FGGDWKGIINKINDGYLTGMGVTAIWISQPVENITSVVNYSGVNNTSYHGYWPRDFKKTN 120 FGGDW+GI NKINDGYLTGMG+TA+WISQPVENIT+V+NYSGVNNT+YHGYWPRDFKKTN Sbjct: 77 FGGDWQGITNKINDGYLTGMGITALWISQPVENITAVINYSGVNNTAYHGYWPRDFKKTN 136 Query: 121 AAFGSFTDFQNLISTAHANNIKVIIDFAPNHTSPASFTDPSFAENGALYNNGTLLGNYTN 180 AAFGSFTDF NLI+ AH++NIKV++DFAPNHT+PAS TDPSFAENGALYNNGTLLG Y+N Sbjct: 137 AAFGSFTDFSNLIAAAHSHNIKVVMDFAPNHTNPASSTDPSFAENGALYNNGTLLGKYSN 196 Query: 181 DSNGLFHHKGGTDFSTIEDGIYRNLYDLADINQNNNTIDKYFKEAIDLWLGLGVDGIRFD 240 D+ GLFHH GGTDFST E GIY+NLYDLADINQNNNTID Y KE+I LWL LGVDGIRFD Sbjct: 197 DTAGLFHHNGGTDFSTTESGIYKNLYDLADINQNNNTIDSYLKESIQLWLNLGVDGIRFD 256 Query: 241 AVKHMPFGWQKSLASSMYSSSHPVFTFGEWFLGPDETSPDNVKFANNSGMNLLDFAYAQE 300 AVKHMP GWQKS SS+YSS++PVFTFGEWFLGPDE + DN+ FAN SGM+LLDFA+AQE Sbjct: 257 AVKHMPQGWQKSYVSSIYSSANPVFTFGEWFLGPDEMTQDNINFANQSGMHLLDFAFAQE 316 Query: 301 VREVFKDKSETMTDLNSVLESTASSYNYIHNMVTFIDNHDMDRFQAAGSSTRPTEQALAL 360 +REVF+DKSETMTDLNSV+ ST SSYNYI+NMVTFIDNHDMDRFQ AG+STRPTEQALA+ Sbjct: 317 IREVFRDKSETMTDLNSVISSTGSSYNYINNMVTFIDNHDMDRFQQAGASTRPTEQALAV 376 Query: 361 TLTSRGVPAIYYGTEQYMTGVGDPYNRAMMTGFNTNTTAYKLIKALAPLRKSNPAIAYGT 420 TLTSRGVPAIYYGTEQYMTG GDP NR MMTGF+TN TAYK+IKALAPLRKSNPA+AYG+ Sbjct: 377 TLTSRGVPAIYYGTEQYMTGNGDPNNRGMMTGFDTNKTAYKVIKALAPLRKSNPALAYGS 436 Query: 421 TTQRWVNNDVYIYERKFGNSVALVAINRNTSTPYPITNLLSSLPAGNYTD 470 TTQRWVN+DVY+YERKFG++VALVA+NR+++T YPI+ L++LP G YTD Sbjct: 437 TTQRWVNSDVYVYERKFGSNVALVAVNRSSTTAYPISGALTALPNGTYTD 486 >gb|AAC04359.1| alpha-cyclodextrin glucanotransferase [Paenibacillus macerans] Length = 714 Score = 789 bits (2038), Expect = 0.0, Method: Compositional matrix adjust. Identities = 375/470 (79%), Positives = 411/470 (87%) Query: 1 MVLGAAVPALADPATSVNNKVNYSTDVIYQIVTDRFVDGNPANNPTGAAFSSDHSNMKLY 60 M LG ++PA A P TSV+NKVN+STDVIYQIVTDRF DG+ NNP G AFS D SN+KLY Sbjct: 17 MALGISLPAWASPDTSVDNKVNFSTDVIYQIVTDRFADGDRTNNPAGDAFSGDRSNLKLY 76 Query: 61 FGGDWKGIINKINDGYLTGMGVTAIWISQPVENITSVVNYSGVNNTSYHGYWPRDFKKTN 120 FGGDW+GII+KINDGYLTGMGVTA+WISQPVENITSV+ YSGVNNTSYHGYW RDFK+TN Sbjct: 77 FGGDWQGIIDKINDGYLTGMGVTALWISQPVENITSVIKYSGVNNTSYHGYWARDFKQTN 136 Query: 121 AAFGSFTDFQNLISTAHANNIKVIIDFAPNHTSPASFTDPSFAENGALYNNGTLLGNYTN 180 AFG F DFQNLI TAHA+NIKV+IDFAPNHTSPA +P FAENGALY+NG+LLG Y+N Sbjct: 137 DAFGDFADFQNLIDTAHAHNIKVVIDFAPNHTSPADRDNPGFAENGALYDNGSLLGAYSN 196 Query: 181 DSNGLFHHKGGTDFSTIEDGIYRNLYDLADINQNNNTIDKYFKEAIDLWLGLGVDGIRFD 240 D+ GLFHH GGTDFSTIEDGIY+NLYDLADIN NNN +D YFK AIDLWLG+GVDGIRFD Sbjct: 197 DTAGLFHHNGGTDFSTIEDGIYKNLYDLADINHNNNAMDAYFKSAIDLWLGMGVDGIRFD 256 Query: 241 AVKHMPFGWQKSLASSMYSSSHPVFTFGEWFLGPDETSPDNVKFANNSGMNLLDFAYAQE 300 AVKHMPFGWQKS SS+Y HPVFTFGEW+LG D+T DN+KFAN SGMNLLDF YAQE Sbjct: 257 AVKHMPFGWQKSFVSSIYGGDHPVFTFGEWYLGADQTDGDNIKFANESGMNLLDFEYAQE 316 Query: 301 VREVFKDKSETMTDLNSVLESTASSYNYIHNMVTFIDNHDMDRFQAAGSSTRPTEQALAL 360 VREVF+DK+ETM DL VL ST S Y+YI+NMVTFIDNHDMDRFQ AGS TR TEQALAL Sbjct: 317 VREVFRDKTETMKDLYEVLASTESQYDYINNMVTFIDNHDMDRFQVAGSGTRATEQALAL 376 Query: 361 TLTSRGVPAIYYGTEQYMTGVGDPYNRAMMTGFNTNTTAYKLIKALAPLRKSNPAIAYGT 420 TLTSRGVPAIYYGTEQYMTG GDP NRAMMT FNT TTAYK+I+ALAPLRKSNPAIAYGT Sbjct: 377 TLTSRGVPAIYYGTEQYMTGDGDPNNRAMMTSFNTGTTAYKVIQALAPLRKSNPAIAYGT 436 Query: 421 TTQRWVNNDVYIYERKFGNSVALVAINRNTSTPYPITNLLSSLPAGNYTD 470 TT+RWVNNDV I ERKFG+S ALVAINRN+S YPI+ LLSSLPAG Y+D Sbjct: 437 TTERWVNNDVLIIERKFGSSAALVAINRNSSAAYPISGLLSSLPAGTYSD 486 >sp|P04830|CDG1_PAEMA Cyclomaltodextrin glucanotransferase precursor (Cyclodextrin-glycosyltransferase) (CGTase) pir||ALBSGR cyclomaltodextrin glucanotransferase (EC 2.4.1.19) precursor - Bacillus macerans emb|CAA41773.1| cyclomaltodextrin glucanotransferase [Paenibacillus macerans] Length = 714 Score = 787 bits (2032), Expect = 0.0, Method: Compositional matrix adjust. Identities = 373/470 (79%), Positives = 410/470 (87%) Query: 1 MVLGAAVPALADPATSVNNKVNYSTDVIYQIVTDRFVDGNPANNPTGAAFSSDHSNMKLY 60 M LG ++PA A P TSV+NKVN+STDVIYQIVTDRF DG+ NNP G AFS D SN+KLY Sbjct: 17 MALGISLPAWASPDTSVDNKVNFSTDVIYQIVTDRFADGDRTNNPAGDAFSGDRSNLKLY 76 Query: 61 FGGDWKGIINKINDGYLTGMGVTAIWISQPVENITSVVNYSGVNNTSYHGYWPRDFKKTN 120 FGGDW+GII+KINDGYLTGMGVTA+WISQPVENITSV+ YSGVNNTSYHGYW RDFK+TN Sbjct: 77 FGGDWQGIIDKINDGYLTGMGVTALWISQPVENITSVIKYSGVNNTSYHGYWARDFKQTN 136 Query: 121 AAFGSFTDFQNLISTAHANNIKVIIDFAPNHTSPASFTDPSFAENGALYNNGTLLGNYTN 180 AFG F DFQNLI TAHA+NIKV+IDFAPNHTSPA +P FAENG +Y+NG+LLG Y+N Sbjct: 137 DAFGDFADFQNLIDTAHAHNIKVVIDFAPNHTSPADRDNPGFAENGGMYDNGSLLGAYSN 196 Query: 181 DSNGLFHHKGGTDFSTIEDGIYRNLYDLADINQNNNTIDKYFKEAIDLWLGLGVDGIRFD 240 D+ GLFHH GGTDFSTIEDGIY+NLYDLADIN NNN +D YFK AIDLWLG+GVDGIRFD Sbjct: 197 DTAGLFHHNGGTDFSTIEDGIYKNLYDLADINHNNNAMDAYFKSAIDLWLGMGVDGIRFD 256 Query: 241 AVKHMPFGWQKSLASSMYSSSHPVFTFGEWFLGPDETSPDNVKFANNSGMNLLDFAYAQE 300 AVKHMPFGWQKS SS+Y HPVFTFGEW+LG D+T DN+KFAN SGMNLLDF YAQE Sbjct: 257 AVKHMPFGWQKSFVSSIYGGDHPVFTFGEWYLGADQTDGDNIKFANESGMNLLDFEYAQE 316 Query: 301 VREVFKDKSETMTDLNSVLESTASSYNYIHNMVTFIDNHDMDRFQAAGSSTRPTEQALAL 360 VREVF+DK+ETM DL VL ST S Y+YI+NMVTFIDNHDMDRFQ AGS TR TEQALAL Sbjct: 317 VREVFRDKTETMKDLYEVLASTESQYDYINNMVTFIDNHDMDRFQVAGSGTRATEQALAL 376 Query: 361 TLTSRGVPAIYYGTEQYMTGVGDPYNRAMMTGFNTNTTAYKLIKALAPLRKSNPAIAYGT 420 TLTSRGVPAIYYGTEQYMTG GDP NRAMMT FNT TTAYK+I+ALAPLRKSNPAIAYGT Sbjct: 377 TLTSRGVPAIYYGTEQYMTGDGDPNNRAMMTSFNTGTTAYKVIQALAPLRKSNPAIAYGT 436 Query: 421 TTQRWVNNDVYIYERKFGNSVALVAINRNTSTPYPITNLLSSLPAGNYTD 470 TT+RWVNNDV I ERKFG+S ALVAINRN+S YPI+ LLSSLPAG Y+D Sbjct: 437 TTERWVNNDVLIIERKFGSSAALVAINRNSSAAYPISGLLSSLPAGTYSD 486 >gb|AAA22298.1| cyclodextrin glucanotransferase Length = 714 Score = 741 bits (1914), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/470 (76%), Positives = 394/470 (83%) Query: 1 MVLGAAVPALADPATSVNNKVNYSTDVIYQIVTDRFVDGNPANNPTGAAFSSDHSNMKLY 60 M LG ++PA A P TSV+NKVN+STDVIYQIVTDRF DG+ NNP G AFS D SN+KLY Sbjct: 17 MALGISLPAWASPDTSVDNKVNFSTDVIYQIVTDRFADGDRTNNPAGDAFSGDRSNLKLY 76 Query: 61 FGGDWKGIINKINDGYLTGMGVTAIWISQPVENITSVVNYSGVNNTSYHGYWPRDFKKTN 120 FGGDW+GII+KINDGYLTGMGVTA+WISQPVENITSV+ YSGVNNTSYHGYW RDFK+TN Sbjct: 77 FGGDWQGIIDKINDGYLTGMGVTALWISQPVENITSVIKYSGVNNTSYHGYWARDFKQTN 136 Query: 121 AAFGSFTDFQNLISTAHANNIKVIIDFAPNHTSPASFTDPSFAENGALYNNGTLLGNYTN 180 AFG F DFQNLI T + H S + T+P FAENGALY+NG+LLG Y+N Sbjct: 137 DAFGDFADFQNLIDTLTLITSRSDRLRPQPHVSGRAGTNPGFAENGALYDNGSLLGAYSN 196 Query: 181 DSNGLFHHKGGTDFSTIEDGIYRNLYDLADINQNNNTIDKYFKEAIDLWLGLGVDGIRFD 240 D+ GLFHH GGTDFSTIEDGIY+NLYDLADIN NNN +D YFK AIDLWLG+GVDGIRFD Sbjct: 197 DTAGLFHHNGGTDFSTIEDGIYKNLYDLADINHNNNAMDAYFKSAIDLWLGMGVDGIRFD 256 Query: 241 AVKHMPFGWQKSLASSMYSSSHPVFTFGEWFLGPDETSPDNVKFANNSGMNLLDFAYAQE 300 AVK PFGWQKS SS+Y HPVFTFGEW+LG D+T DN+KFAN SGMNLLDF YAQE Sbjct: 257 AVKQYPFGWQKSFVSSIYGGDHPVFTFGEWYLGADQTDGDNIKFANESGMNLLDFEYAQE 316 Query: 301 VREVFKDKSETMTDLNSVLESTASSYNYIHNMVTFIDNHDMDRFQAAGSSTRPTEQALAL 360 VREVF+DK+ETM DL VL ST S Y+YI+NMVTFIDNHDMDRFQ AGS TR TEQALAL Sbjct: 317 VREVFRDKTETMKDLYEVLASTESQYDYINNMVTFIDNHDMDRFQVAGSGTRATEQALAL 376 Query: 361 TLTSRGVPAIYYGTEQYMTGVGDPYNRAMMTGFNTNTTAYKLIKALAPLRKSNPAIAYGT 420 TLTSRGVPAIYYGTEQYMTG GDP NRAMMT FNT TTAYK+I+ALAPLRKSNPAIAYGT Sbjct: 377 TLTSRGVPAIYYGTEQYMTGDGDPNNRAMMTSFNTGTTAYKVIQALAPLRKSNPAIAYGT 436 Query: 421 TTQRWVNNDVYIYERKFGNSVALVAINRNTSTPYPITNLLSSLPAGNYTD 470 TT+RWVNNDV I ERKFG+S ALVAINRN+S YPI+ LLSSLPAG Y+D Sbjct: 437 TTERWVNNDVLIIERKFGSSAALVAINRNSSAAYPISGLLSSLPAGTYSD 486 >sp|P26827|CDGT_THETU Cyclomaltodextrin glucanotransferase precursor (Cyclodextrin-glycosyltransferase) (CGTase) pir||S63598 cyclomaltodextrin glucanotransferase (EC 2.4.1.19) precursor [validated] - Thermoanaerobacterium thermosulfurigenes (strain EM1) gb|AAB00845.1| alpha-cyclodextrin glycosyltransferase [Thermoanaerobacterium thermosulfurigenes] Length = 710 Score = 705 bits (1819), Expect = 0.0, Method: Compositional matrix adjust. Identities = 344/471 (73%), Positives = 395/471 (83%), Gaps = 3/471 (0%) Query: 1 MVLGAAVPALADPATSVNNKVNYSTDVIYQIVTDRFVDGNPANNPTGAAFSSDHSNMKLY 60 +V G P A T+V+N VNYSTDVIYQIVTDRFVDGN +NNPTG + H+++K Y Sbjct: 17 LVFGLTAPIQAASDTAVSNVVNYSTDVIYQIVTDRFVDGNTSNNPTGDLYDPTHTSLKKY 76 Query: 61 FGGDWKGIINKINDGYLTGMGVTAIWISQPVENITSVVNYSGVN-NTSYHGYWPRDFKKT 119 FGGDW+GIINKINDGYLTGMGVTAIWISQPVENI +V+ S +TSYHGYW RDFK+T Sbjct: 77 FGGDWQGIINKINDGYLTGMGVTAIWISQPVENIYAVLPDSTFGGSTSYHGYWARDFKRT 136 Query: 120 NAAFGSFTDFQNLISTAHANNIKVIIDFAPNHTSPASFTDPSFAENGALYNNGTLLGNYT 179 N FGSFTDFQNLI+TAHA+NIKVIIDFAPNHTSPAS TDP++AENG LY+NGTLLG YT Sbjct: 137 NPYFGSFTDFQNLINTAHAHNIKVIIDFAPNHTSPASETDPTYAENGRLYDNGTLLGGYT 196 Query: 180 NDSNGLFHHKGGTDFSTIEDGIYRNLYDLADINQNNNTIDKYFKEAIDLWLGLGVDGIRF 239 ND+NG FHH GGTDFS+ EDGIYRNL+DLAD+NQ N+TID Y K AI +WL +G+DGIR Sbjct: 197 NDTNGYFHHYGGTDFSSYEDGIYRNLFDLADLNQQNSTIDSYLKSAIKVWLDMGIDGIRL 256 Query: 240 DAVKHMPFGWQKSLASSMYSSSHPVFTFGEWFLGPDETSPDNVKFANNSGMNLLDFAYAQ 299 DAVKHMPFGWQK+ S+ S PVFTFGEWFLG +E +N FAN SGM+LLDF ++Q Sbjct: 257 DAVKHMPFGWQKNFMDSIL-SYRPVFTFGEWFLGTNEIDVNNTYFANESGMSLLDFRFSQ 315 Query: 300 EVREVFKDKSETMTDLNSVLESTASSYNYIHNMVTFIDNHDMDRFQAAGSSTRPTEQALA 359 +VR+VF+D ++TM L+S+++STAS YN+I++MVTFIDNHDMDRF G STRP EQALA Sbjct: 316 KVRQVFRDNTDTMYGLDSMIQSTASDYNFINDMVTFIDNHDMDRFYNGG-STRPVEQALA 374 Query: 360 LTLTSRGVPAIYYGTEQYMTGVGDPYNRAMMTGFNTNTTAYKLIKALAPLRKSNPAIAYG 419 TLTSRGVPAIYYGTEQYMTG GDPYNRAMMT FNT+TTAY +IK LAPLRKSNPAIAYG Sbjct: 375 FTLTSRGVPAIYYGTEQYMTGNGDPYNRAMMTSFNTSTTAYNVIKKLAPLRKSNPAIAYG 434 Query: 420 TTTQRWVNNDVYIYERKFGNSVALVAINRNTSTPYPITNLLSSLPAGNYTD 470 TT QRW+NNDVYIYERKFGN+VALVAINRN ST Y IT L ++LPAG YTD Sbjct: 435 TTQQRWINNDVYIYERKFGNNVALVAINRNLSTSYNITGLYTALPAGTYTD 485 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.132 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 818,338,964 Number of extensions: 36312472 Number of successful extensions: 107426 Number of sequences better than 1.0e-05: 620 Number of HSP's gapped: 106553 Number of HSP's successfully gapped: 658 Length of query: 529 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 399 Effective length of database: 404,274,708 Effective search space: 161305608492 Effective search space used: 161305608492 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)