BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0056.p (201 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|Q45633|YMA3_BACST Hypothetical 35.7 kDa protein in MALA 3'reg... 101 1e-20 ref|NP_834405.1| 2'-5' RNA ligase [Bacillus cereus ATCC 14579] >... 97 2e-19 ref|ZP_00019417.1| hypothetical protein [Chloroflexus aurantiacus] 93 3e-18 ref|NP_658732.1| 2_5_ligase, 2',5' RNA ligase family [Bacillus a... 91 2e-17 gb|EAJ55483.1| unknown [environmental sequence] 89 5e-17 >sp|Q45633|YMA3_BACST Hypothetical 35.7 kDa protein in MALA 3'region (ORF3) pir||S43916 hypothetical protein 3 - Bacillus stearothermophilus gb|AAA71981.1| [malA] gene products Length = 314 Score = 101 bits (251), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 65/189 (34%), Positives = 87/189 (46%), Gaps = 10/189 (5%) Query: 10 RERLFIALKLPFALCEKLEKERSVLSGKLNFAKWTHSEDYHITLQFLGDTPKRSIPGLLE 69 R FIA+ L + + + S L F W H EDYHITL FLGD P + L E Sbjct: 3 RSHYFIAVPLTSEAKQAISRFSGDASSSLPFRTWVHEEDYHITLAFLGDVPPGKMAPLCE 62 Query: 70 ALKEIPAACQPFQLQLSEWGVFGRPESPKVLWAGVSGEMEKLKDLQKRVISVTRPLGFIP 129 A+ + A PF L L+ G FG +P++ W GV E L +L++ V LGF Sbjct: 63 AMAAVAAKSAPFSLALAGLGTFGERTAPRIFWQGVKEEA-ALNELRRDVYEACLSLGFSL 121 Query: 130 ETREFKPHLTLARKYRNELPFSXXXXXXXXXXXXXXXXKTGIADWTVDGFVVYATRMHAI 189 + R F PH+T+ARK++ E PF ++V V+Y T M Sbjct: 122 DRRPFAPHITIARKWQGEAPFQPEALRSLPAASTV---------FSVPEIVLYRTNMEKT 172 Query: 190 PMYEMIEKF 198 P YE I F Sbjct: 173 PKYETIAAF 181 >ref|NP_834405.1| 2'-5' RNA ligase [Bacillus cereus ATCC 14579] gb|AAP11606.1| 2'-5' RNA ligase [Bacillus cereus ATCC 14579] Length = 181 Score = 97.1 bits (240), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 52/137 (37%), Positives = 73/137 (53%), Gaps = 2/137 (1%) Query: 14 FIALKLPFALCEKLEKERSVLSGKLNFAKWTHSEDYHITLQFLGDTPKRSIPGLLEALKE 73 F+A+ LP + E L + + +L F W H EDYHITL FLG + G+ LK Sbjct: 6 FVAITLPNHIKEMLSNYKEDMQEELQFRSWVHKEDYHITLSFLGSATGEQLEGIKNGLKT 65 Query: 74 IPAACQPFQLQLSEWGVFGRPESPKVLWAGVSGEMEKLKDLQKRVISVTRPLGFIPETRE 133 I A + L + FGR + P++ WA VS E L LQK++ ++ GF ETR Sbjct: 66 I-AETKELSFTLQGFSTFGREDQPRIFWAKVS-ENHDLFQLQKQIHAICEENGFSLETRP 123 Query: 134 FKPHLTLARKYRNELPF 150 + PH+T+ARK+ E F Sbjct: 124 YHPHITVARKWIGEEKF 140 >ref|ZP_00019417.1| hypothetical protein [Chloroflexus aurantiacus] Length = 190 Score = 93.2 bits (230), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 51/135 (37%), Positives = 76/135 (56%), Gaps = 2/135 (1%) Query: 12 RLFIALKLPFALCEKLEKERSVLSGKLNFAKWTHSEDYHITLQFLGDTPKRSIPGLLEAL 71 RLFIAL+LP + ++L++ + L G A+WT H+TL FLG+TP+R + LL L Sbjct: 12 RLFIALELPQPVRDELKRIQQRLQGHSRAARWTDPTGMHLTLHFLGETPERLVEPLLTGL 71 Query: 72 KEIPAACQPFQLQLSEWGVFGRPESPKVLWAGVSGEMEKLKDLQKRVISVTRPLGFIPET 131 + + A L L G F + +V+W G+ G++ L L + + +V + LG ET Sbjct: 72 RSVSAPA--LSLTLDRLGCFPANGALRVVWVGLRGDLAALATLHRSLATVIQSLGLPTET 129 Query: 132 REFKPHLTLARKYRN 146 R F PH+TLAR N Sbjct: 130 RPFVPHITLARARPN 144 >ref|NP_658732.1| 2_5_ligase, 2',5' RNA ligase family [Bacillus anthracis A2012] ref|NP_847152.1| 2'-5' RNA ligase family protein, putative [Bacillus anthracis str. Ames] gb|AAP28638.1| 2'-5' RNA ligase family protein, putative [Bacillus anthracis str. Ames] Length = 181 Score = 90.5 bits (223), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 50/138 (36%), Positives = 74/138 (53%), Gaps = 2/138 (1%) Query: 14 FIALKLPFALCEKLEKERSVLSGKLNFAKWTHSEDYHITLQFLGDTPKRSIPGLLEALKE 73 F+A+ LP + E L R + +L F W H EDYHITL FLG + + G+ L+ Sbjct: 6 FVAITLPNHIKEVLSNYREEMQEELPFRSWVHKEDYHITLSFLGSATEEQLEGIQNGLQT 65 Query: 74 IPAACQPFQLQLSEWGVFGRPESPKVLWAGVSGEMEKLKDLQKRVISVTRPLGFIPETRE 133 + + L + FG + P++ WA VS E + L LQK+V ++ GF ETR Sbjct: 66 L-IETKELSFTLQGFSTFGMEDRPRIFWAKVS-ENQDLFPLQKQVHAICEGNGFSLETRP 123 Query: 134 FKPHLTLARKYRNELPFS 151 + PH+T+ARK+ E F+ Sbjct: 124 YHPHITVARKWVGEEKFN 141 >gb|EAJ55483.1| unknown [environmental sequence] Length = 192 Score = 89.4 bits (220), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 43/131 (32%), Positives = 74/131 (56%) Query: 12 RLFIALKLPFALCEKLEKERSVLSGKLNFAKWTHSEDYHITLQFLGDTPKRSIPGLLEAL 71 R FIA+ P + K S + W + H+T++FLG TP+ +I ++E + Sbjct: 7 RTFIAIPTPVEVRSKKNMLYSTIEDSPATINWVKNMQLHLTIKFLGHTPESTINKIIEDV 66 Query: 72 KEIPAACQPFQLQLSEWGVFGRPESPKVLWAGVSGEMEKLKDLQKRVISVTRPLGFIPET 131 +++ + +PF++ + G F PE P+ LW GV+G ++ L +L ++ + LGF E Sbjct: 67 EKLTSGLKPFEITIESTGCFPVPERPRTLWMGVTGNLDPLNELFMKIENSLELLGFPKEE 126 Query: 132 REFKPHLTLAR 142 +E+KPH+TLAR Sbjct: 127 QEYKPHITLAR 137 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.138 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 276,495,816 Number of extensions: 10247191 Number of successful extensions: 20159 Number of sequences better than 1.0e-05: 77 Number of HSP's gapped: 20117 Number of HSP's successfully gapped: 77 Length of query: 201 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 81 Effective length of database: 431,425,698 Effective search space: 34945481538 Effective search space used: 34945481538 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 123 (52.0 bits)