BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0029.p (518 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061978.1| COG0119: Isopropylmalate/homocitrate/citramal... 450 e-125 ref|NP_228363.1| 2-isopropylmalate synthase [Thermotoga maritima... 444 e-123 gb|EAI15937.1| unknown [environmental sequence] 440 e-122 gb|EAI11615.1| unknown [environmental sequence] 439 e-122 ref|NP_578666.1| 2-isopropylmalate synthase [Pyrococcus furiosus... 429 e-119 >ref|ZP_00061978.1| COG0119: Isopropylmalate/homocitrate/citramalate synthases [Clostridium thermocellum ATCC 27405] Length = 511 Score = 450 bits (1157), Expect = e-125, Method: Compositional matrix adjust. Identities = 225/495 (45%), Positives = 322/495 (65%), Gaps = 4/495 (0%) Query: 7 KRIQIFDTTLRDGEQAPGASLTPEQKIILAYKLAELGVDVLEPGFPVSSPGDFAAVETIS 66 +RI+IFDTTLRDGEQ PG +L ++K+ +A +LA LGVDV+EPGFP++SPGDF AV+ I+ Sbjct: 2 RRIRIFDTTLRDGEQTPGVNLNIQEKVDIAKQLARLGVDVIEPGFPLTSPGDFEAVQRIA 61 Query: 67 RKVQGVEICGFARAVRGDIDAAVRATRDAERRRLHLFISSSDIHLRHQLRMSRAEVVAAA 126 R+V+G ICGF+RA+ DID +A +DA+++ H+FISSSDI ++HQL + +V+ Sbjct: 62 REVEGPYICGFSRAIIRDIDETWKAIKDAQKKCFHIFISSSDIQIKHQLGKTEKDVLEIV 121 Query: 127 REMTAYARQFCEVVEFTAMDAARTGIDDLIEMVEAVIEEGATIINLPDTVGYALPQEYGE 186 + +A+Q+ + VE++ MDA+RT ++ L E++EA I+ GAT+IN+PDTVGYA P E+GE Sbjct: 122 KSTVYHAKQYTDEVEYSPMDASRTRLEFLYEVIEAAIDNGATVINIPDTVGYATPIEFGE 181 Query: 187 MFRRVRQGARGGDTVSYSAHCHNDLGLAVANSLAAIEGGATQIEVTVNGVGERTGNCALE 246 + +++R+ R D S HCHNDLG+AVANS+ A GA QIE T+NGVGER GN ALE Sbjct: 182 LIQKIRKNVRNIDKAIISVHCHNDLGMAVANSIVAAMNGAQQIECTINGVGERAGNAALE 241 Query: 247 ELVMALETRGDALGAETGIVLDKLYDTSRIISGAMHFPIAYNKPVVGRNAFQHESGIHQD 306 E+V + R D LG ETGI L +LY TS+I+S M PI NKP+VG+N F HESGIHQD Sbjct: 242 EVVTHIAARKDYLGFETGIDLSQLYKTSKIVSRYMGIPIPVNKPIVGKNVFTHESGIHQD 301 Query: 307 GLLKDRSTYEIMDPERLGIPRSMIILGKHSGRHALKDRAAKYGIALSPGELDALYESFKE 366 G+LK+RSTYE++DP +G S+I+LGKHSGRHALK A K G L L+ L+ FK+ Sbjct: 302 GVLKERSTYEVIDPRLVGRDDSVILLGKHSGRHALKVEAEKLGYDLDEERLNKLFNDFKK 361 Query: 367 TADRQKAVSDDELLRMVSTTTGQEA-KVYELGEVQVLAGSAQRRVAAVTVRHLKSGEETS 425 D +K V+ +L ++ + + + Y L +++V++G+ + A V ++ K G Sbjct: 362 LTDVKKNVTTADLESLIIESAAKAVEEAYVLEKIRVVSGNIETPSAKVVIKDSK-GNLLE 420 Query: 426 HTSTGNGPLXXXXXXXXXXXXXXXXFTGLELHSLGSGENAQA--EAAVMVEWEGNKFRGT 483 TGNGP+ T + E ++ E +V + + + G Sbjct: 421 AEQTGNGPVDAVFKAINSVIKETENLTLYKYSVSAVTEEMESLGEVSVTLREKEKLYTGI 480 Query: 484 AIHQDIVMAAGIAYI 498 H DI+ ++ IAYI Sbjct: 481 GTHTDIITSSAIAYI 495 >ref|NP_228363.1| 2-isopropylmalate synthase [Thermotoga maritima] sp|Q9WZ23|LEU1_THEMA 2-isopropylmalate synthase (Alpha-isopropylmalate synthase) (Alpha-IPM synthetase) pir||G72362 2-isopropylmalate synthase - Thermotoga maritima (strain MSB8) gb|AAD35638.1| 2-isopropylmalate synthase [Thermotoga maritima] Length = 513 Score = 444 bits (1141), Expect = e-123, Method: Compositional matrix adjust. Identities = 228/495 (46%), Positives = 313/495 (63%), Gaps = 6/495 (1%) Query: 7 KRIQIFDTTLRDGEQAPGASLTPEQKIILAYKLAELGVDVLEPGFPVSSPGDFAAVETIS 66 +RI+IFDTTLRDGEQ+PGAS++ E+K+ +A L +LGVD++E GFPVSSP F AV+ ++ Sbjct: 2 RRIKIFDTTLRDGEQSPGASMSIEEKVEMALMLEDLGVDLIEAGFPVSSPVQFEAVKRVA 61 Query: 67 RKVQGVEICGFARAVRGDIDAAVRATRD--AERRRLHLFISSSDIHLRHQLRMSRAEVVA 124 VQ + G AR V DIDAA A +D ++R +H+FI++S IH +++LRM + E++ Sbjct: 62 SAVQKPIVVGLARCVEKDIDAAYEALKDRPKDKRMIHVFIATSPIHRKYKLRMEKEEILE 121 Query: 125 AAREMTAYARQFCEVVEFTAMDAARTGIDDLIEMVEAVIEEGATIINLPDTVGYALPQEY 184 R YA+QF ++VEF+A DA+RT + LIE + IE GAT IN+PDTVGYALP E+ Sbjct: 122 RIRRYVGYAKQFFDLVEFSAEDASRTEVPFLIEAYKTAIEAGATTINVPDTVGYALPDEF 181 Query: 185 GEMFRRVRQGARGGDTVSYSAHCHNDLGLAVANSLAAIEGGATQIEVTVNGVGERTGNCA 244 GE+ + +R+G G + V S HCHNDLGLAVANSLAA++ GATQ+EVT+NG+GER GNCA Sbjct: 182 GELIKTLREGVPGIENVDLSVHCHNDLGLAVANSLAAVQNGATQVEVTLNGIGERAGNCA 241 Query: 245 LEELVMALETRGDALGAETGIVLDKLYDTSRIISGAMHFPIAYNKPVVGRNAFQHESGIH 304 LEE VM L+ R D L ETGI + +Y SR+++ + NKP+VG N F HESGIH Sbjct: 242 LEEFVMILKVRKDKLPYETGIKTELIYPASRLLTHITGLIPSRNKPIVGENVFLHESGIH 301 Query: 305 QDGLLKDRSTYEIMDPERLGIPRSMIILGKHSGRHALKDRAAKYGIALSPGELDALYESF 364 QDG+LK R TYEIM P +G ++LG+HSG+HAL+ + YGI L ++E F Sbjct: 302 QDGVLKHRETYEIMKPSDIGRSSETLVLGRHSGKHALRKKLETYGIKLDEETFQKVFEKF 361 Query: 365 KETADRQKAVSDDELLRMVSTTTGQEAKVYELGEVQVLAGSAQRRVAAVTVRHLKSGEET 424 E ADR+K V DD+L +VS + Y+L V G+ AAV L+ G E Sbjct: 362 TELADRKKEVYDDDLFSIVSEVLREPINGYKLVHFHVHTGNTLLPTAAVV---LQVGNEK 418 Query: 425 SHTS-TGNGPLXXXXXXXXXXXXXXXXFTGLELHSLGSGENAQAEAAVMVEWEGNKFRGT 483 + GNGP+ + ++G+G+NAQ E + + + + G Sbjct: 419 REAAEAGNGPVDAIFKAIDKALGIQPKLEEYIIQAVGTGKNAQGEVKLTLRIDNELYSGR 478 Query: 484 AIHQDIVMAAGIAYI 498 + DIV A+ IAYI Sbjct: 479 GVSTDIVEASAIAYI 493 >gb|EAI15937.1| unknown [environmental sequence] Length = 511 Score = 440 bits (1131), Expect = e-122, Method: Compositional matrix adjust. Identities = 222/498 (44%), Positives = 315/498 (63%), Gaps = 10/498 (2%) Query: 7 KRIQIFDTTLRDGEQAPGASLTPEQKIILAYKLAELGVDVLEPGFPVSSPGDFAAVETIS 66 + I IFDTTLRDGEQAPG S+T +K+ +A+ L EL VDV+E GF +SPGDF AV I+ Sbjct: 5 REIIIFDTTLRDGEQAPGCSMTMGEKLKIAHALKELNVDVIEAGFAAASPGDFDAVNAIA 64 Query: 67 RKVQGVEICGFARAVRGDIDAAVRATRDAERRRLHLFISSSDIHLRHQLRMSRAEVVAAA 126 ++ G IC AR DI+ A A + + RR+H+F+++S+IHL+H+L M++ EV+ AA Sbjct: 65 SEIDGPTICSLARCNESDIEKAYEALKVNKNRRVHVFVATSEIHLKHKLNMAKDEVLKAA 124 Query: 127 REMTAYARQFCEVVEFTAMDAARTGIDDLIEMVEAVIEEGATIINLPDTVGYALPQEYGE 186 E A+ FCE VEF+ DA+RT L E+V A IE GAT IN+PDTVGY +P E+GE Sbjct: 125 YEGVKMAKSFCENVEFSPEDASRTDHGFLTEVVNAAIEAGATTINVPDTVGYTIPYEFGE 184 Query: 187 MFRRVRQGARGGDTVSYSAHCHNDLGLAVANSLAAIEGGATQIEVTVNGVGERTGNCALE 246 +F+ + + V +S HCH+DLGL+VANSLAA+E GA QIE T+NG+GER GN +LE Sbjct: 185 LFKHLISNCNDAENVIFSVHCHDDLGLSVANSLAAVESGARQIECTINGIGERAGNTSLE 244 Query: 247 ELVMALETRGDALGAETGIVLDKLYDTSRIISG--AMHFPIAYNKPVVGRNAFQHESGIH 304 E+ MA+ TR + +T I KLY TSR++S MH P NK +VG NAF HE+GIH Sbjct: 245 EVCMAIRTREEYFDFKTSIDSTKLYPTSRLVSSITGMHVP--RNKAIVGDNAFAHEAGIH 302 Query: 305 QDGLLKDRSTYEIMDPERLGIPRSMIILGKHSGRHALKDRAAKYGIALSPGELDALYESF 364 Q G+L+D STYEIM P+ +GI RS ++LGKHSGRHA DR + G AL E+D ++ F Sbjct: 303 QHGMLRDSSTYEIMKPQDVGIARSNLVLGKHSGRHAFLDRINELGFALEKDEIDPAFQEF 362 Query: 365 KETADRQKAVSDDELLRMVSTTTGQEAKVYELGEVQVLAGSAQRRVAAVTVRHLKSGEET 424 K+ ADR+K + D ++ +V + L +++ +GS+ A++ ++ SGEE Sbjct: 363 KKLADRKKEIFDTDIEAIVLNADIANMSRWTLMDLETTSGSSWGSTASLKLK--DSGEEI 420 Query: 425 ----SHTSTGNGPLXXXXXXXXXXXXXXXXFTGLELHSLGSGENAQAEAAVMVEWEGNKF 480 ++++ +GP+ T E+HS+ G++AQ E V V + G + Sbjct: 421 IERDNNSNPASGPIEAVFNIISDITGYKLELTNFEIHSVSVGDDAQGEVTVTVSYNGQNY 480 Query: 481 RGTAIHQDIVMAAGIAYI 498 RG I DI+ + +AY+ Sbjct: 481 RGHGISTDIIESGALAYL 498 >gb|EAI11615.1| unknown [environmental sequence] Length = 511 Score = 439 bits (1130), Expect = e-122, Method: Compositional matrix adjust. Identities = 222/498 (44%), Positives = 315/498 (63%), Gaps = 10/498 (2%) Query: 7 KRIQIFDTTLRDGEQAPGASLTPEQKIILAYKLAELGVDVLEPGFPVSSPGDFAAVETIS 66 + I IFDTTLRDGEQAPG S+T +K+ +A+ L EL VDV+E GF +SPGDF AV I+ Sbjct: 5 REIIIFDTTLRDGEQAPGCSMTMGEKLKIAHALKELNVDVIEAGFAAASPGDFDAVNAIA 64 Query: 67 RKVQGVEICGFARAVRGDIDAAVRATRDAERRRLHLFISSSDIHLRHQLRMSRAEVVAAA 126 ++ G IC AR DI+ A A + + RR+H+F+++S+IHL+H+L M++ EV+ AA Sbjct: 65 SEIDGPTICSLARCNESDIEKAYEALKVNKNRRVHVFVATSEIHLKHKLNMAKDEVLKAA 124 Query: 127 REMTAYARQFCEVVEFTAMDAARTGIDDLIEMVEAVIEEGATIINLPDTVGYALPQEYGE 186 E A+ FCE VEF+ DA+RT L E+V A IE GAT IN+PDTVGY +P E+GE Sbjct: 125 YEGVKMAKSFCENVEFSPEDASRTDHGFLTEVVNAAIEAGATTINVPDTVGYTIPYEFGE 184 Query: 187 MFRRVRQGARGGDTVSYSAHCHNDLGLAVANSLAAIEGGATQIEVTVNGVGERTGNCALE 246 +F+ + + V +S HCH+DLGL+VANSLAA+E GA QIE T+NG+GER GN +LE Sbjct: 185 LFKHLISNCNDAENVIFSVHCHDDLGLSVANSLAAVESGARQIECTINGIGERAGNTSLE 244 Query: 247 ELVMALETRGDALGAETGIVLDKLYDTSRIISG--AMHFPIAYNKPVVGRNAFQHESGIH 304 E+ MA+ TR + +T I KLY TSR++S MH P NK +VG NAF HE+GIH Sbjct: 245 EVCMAIRTREEYFDFKTSIDSTKLYPTSRLVSSITGMHVP--RNKAIVGDNAFAHEAGIH 302 Query: 305 QDGLLKDRSTYEIMDPERLGIPRSMIILGKHSGRHALKDRAAKYGIALSPGELDALYESF 364 Q G+L+D STYEIM P+ +GI RS ++LGKHSGRHA DR + G AL E+D ++ F Sbjct: 303 QHGMLRDSSTYEIMKPQDVGIARSNLVLGKHSGRHAFLDRIDELGFALEKDEIDPAFQEF 362 Query: 365 KETADRQKAVSDDELLRMVSTTTGQEAKVYELGEVQVLAGSAQRRVAAVTVRHLKSGEET 424 K+ ADR+K + D ++ +V + L +++ +GS+ A++ ++ SGEE Sbjct: 363 KKLADRKKEIFDTDIEAIVLNADIANMSRWTLMDLETTSGSSWGSTASLKLK--DSGEEI 420 Query: 425 ----SHTSTGNGPLXXXXXXXXXXXXXXXXFTGLELHSLGSGENAQAEAAVMVEWEGNKF 480 ++++ +GP+ T E+HS+ G++AQ E V V + G + Sbjct: 421 IERDNNSNPASGPIEAVFNIISDITGYKLELTNFEIHSVSVGDDAQGEVTVTVSYNGQNY 480 Query: 481 RGTAIHQDIVMAAGIAYI 498 RG I DI+ + +AY+ Sbjct: 481 RGHGISTDIIESGALAYL 498 >ref|NP_578666.1| 2-isopropylmalate synthase [Pyrococcus furiosus DSM 3638] sp|Q8U2A2|LEU1_PYRFU 2-isopropylmalate synthase (Alpha-isopropylmalate synthase) (Alpha-IPM synthetase) gb|AAL81061.1| 2-isopropylmalate synthase; (leuA) [Pyrococcus furiosus DSM 3638] Length = 499 Score = 429 bits (1103), Expect = e-119, Method: Compositional matrix adjust. Identities = 223/493 (45%), Positives = 320/493 (64%), Gaps = 5/493 (1%) Query: 7 KRIQIFDTTLRDGEQAPGASLTPEQKIILAYKLAELGVDVLEPGFPVSSPGDFAAVETIS 66 ++I+IFDTTLRDGEQ PG SLT E+KI +A +LA L VDV+E GFP+SSPG+F AV+ I+ Sbjct: 3 RKIKIFDTTLRDGEQTPGVSLTVEEKIEIAKQLARLNVDVIEAGFPISSPGEFEAVKRIA 62 Query: 67 RKVQGVEICGFARAVRGDIDAAVRATRDAERRRLHLFISSSDIHLRHQLRMSRAEVVAAA 126 R+V+G I ARAV+ DIDAA A +DAE +R+H FI++S IH++++LR + EV A Sbjct: 63 REVRGPTIAALARAVKKDIDAAGEALKDAESKRIHTFIATSLIHMKYKLRKTPEEVKKMA 122 Query: 127 REMTAYARQFCEVVEFTAMDAARTGIDDLIEMVEAVIEEGATIINLPDTVGYALPQEYGE 186 E YA ++ + +EF+A DA R+ + L+E+ EAVI+ GAT IN+PDTVGY P+E+ E Sbjct: 123 VEAVEYATKYTDDIEFSAEDATRSDWNFLVEVYEAVIDAGATTINVPDTVGYTTPEEFYE 182 Query: 187 MFRRVRQGARGGDTVSYSAHCHNDLGLAVANSLAAIEGGATQIEVTVNGVGERTGNCALE 246 + R +++ + V+ S HCHNDLGLAVANSL+A+ GA Q+EVTVNG+GER GN ALE Sbjct: 183 LIRYLKRNITNLNGVTISVHCHNDLGLAVANSLSAVRAGADQVEVTVNGIGERAGNAALE 242 Query: 247 ELVMALETRGDALGAETGIVLDKLYDTSRIISGAMHFPIAYNKPVVGRNAFQHESGIHQD 306 E+V+AL+ R D ETGI L ++ TS++++ + NK VVG NAF HESGIHQD Sbjct: 243 EVVVALDVRKDFYNVETGINLGEIARTSKLVARLTGIEVPPNKAVVGANAFAHESGIHQD 302 Query: 307 GLLKDRSTYEIMDPERLGIPRSMIILGKHSGRHALKDRAAKYGIALSPGELDALYESFKE 366 G+LK+R+TYEI+DP +LG S I+LGKHSGRHA + + + G LS E++ L+ FKE Sbjct: 303 GVLKERTTYEIIDPRKLGFSGSKIVLGKHSGRHAFRKKLEEMGYRLSEEEINRLFAKFKE 362 Query: 367 TADRQKAVSDDELLRMVSTTTGQEAKVYELGEVQVLAGSAQRRVAAVTVRHLKSG-EETS 425 ADR+K +++ ++ +V G+ Y + + V++G +++ TVR G ++ Sbjct: 363 IADRKKGLTELDIEAIVQEELGKGKGKYSVEVLHVISG----KISTATVRVQGDGIDKIE 418 Query: 426 HTSTGNGPLXXXXXXXXXXXXXXXXFTGLELHSLGSGENAQAEAAVMVEWEGNKFRGTAI 485 + NGP+ + S+ SG +A E V VE+ G + G + Sbjct: 419 SAWSSNGPIDALFAAINRALGIDCKLKEYRVSSVTSGRDALGEVLVRVEYNGEIYVGRGL 478 Query: 486 HQDIVMAAGIAYI 498 DI+ A+ AY+ Sbjct: 479 STDIIEASAQAYL 491 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 663,508,749 Number of extensions: 26121253 Number of successful extensions: 72325 Number of sequences better than 1.0e-05: 1630 Number of HSP's gapped: 70333 Number of HSP's successfully gapped: 1630 Length of query: 518 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 388 Effective length of database: 404,274,708 Effective search space: 156858586704 Effective search space used: 156858586704 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)