BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0032.p (336 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAF18519.1| 2-oxoglutarate synthase, 2-oxoacid-ferredoxin ox... 115 1e-24 ref|NP_276850.1| pyruvate oxidoreductase, gamma subunit [Methano... 112 1e-23 ref|NP_227831.1| pyruvate ferredoxin oxidoreductase, gamma subun... 99 1e-19 emb|CAA59455.1| gamma subunit of pyruvate ferredoxin oxidoreduct... 98 3e-19 ref|ZP_00059749.1| COG1014: Pyruvate:ferredoxin oxidoreductase a... 89 2e-16 >emb|CAF18519.1| 2-oxoglutarate synthase, 2-oxoacid-ferredoxin oxidoreductase CD subunit [Thermoproteus tenax] Length = 311 Score = 115 bits (288), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 94/308 (30%), Positives = 140/308 (45%), Gaps = 20/308 (6%) Query: 21 GGLGANLAGKMLAEAGVVGAGLNGVSFSSYGSEKKGSAVKAHIRFCDL--NTPIRDTSPV 78 GG G A ++A A + G ++ +G+E++G+ VKA +R D + P P+ Sbjct: 11 GGQGIVTAVYIVAHASIAD-GFYALANPEFGAERRGAPVKAFLRISDTFEDAP----EPI 65 Query: 79 ERPHVVGVFHEALAKTVNVT-SGILEHSTVLVNSAKSPAELKELLKMKAGTIAVIDATSI 137 + P V + + L + V + VLV+S K P ++ EL+ K IAV+D I Sbjct: 66 KTPDVAVILDDKLIDPMRVVIDAVKPGGYVLVSSGKDPEKVAELIGRKDVNIAVVDGIGI 125 Query: 138 ALKEKNRV--NMAMLGALFRLCPFLDTETMKGVIEKSLGKKYPQAVQSAISTFERGYNEV 195 ALK N M+G R+ F +++K IE LGK Q +A ++ Sbjct: 126 ALKHVKLAVPNGPMVGVFARVMGFPKLQSIKEAIESQLGKAVEQNFAAAEEAYKTAVIIP 185 Query: 196 EFMQFELAAGDSMPE---YVRSDIGVLGYDTQPMGGSIINPGSTFLKNLSISRSGMLPAF 252 Q A +P ++ D ++G+D P GG + PGS+ L+ + S P Sbjct: 186 ATEQGTAKAAVEIPTTSAFLTGDYELVGWDKIPEGG-LTYPGSS-LRYKTGSWRVEKPII 243 Query: 253 DLEACIHCAQCDTVCPDQCF--VWEERVDRKGRSQMYLTGID--YQYCKGCLKCVGACPT 308 D CI C +C CPD VW E GR + + ID Y+YCKGC C CPT Sbjct: 244 DHSKCIMCRKCWMFCPDDAVLEVWRETKLPSGRV-VRVKAIDFSYEYCKGCGICADVCPT 302 Query: 309 SALSSQRE 316 A+ RE Sbjct: 303 GAIQMVRE 310 >ref|NP_276850.1| pyruvate oxidoreductase, gamma subunit [Methanothermobacter thermautotrophicus] pir||G69099 probable pyruvate synthase (EC 1.2.7.1) gamma chain - Methanobacterium thermoautotrophicum (strain Delta H) gb|AAB86210.1| pyruvate oxidoreductase, gamma subunit [Methanothermobacter thermautotrophicus str. Delta H] Length = 261 Score = 112 bits (280), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 91/314 (28%), Positives = 137/314 (43%), Gaps = 61/314 (19%) Query: 12 FFEIRLESIGGLGANLAGKMLAEAGVVGAGLNGVSFSSYGSEKKGSAVKAHIRFCDLNTP 71 EIR GG GA A ++LA+A G +F +G E++G+ V A R D +P Sbjct: 1 MIEIRFHGRGGQGAVTAAEILAKAAF-EDGKYSQAFPFFGVERRGAPVMAFTRIDD--SP 57 Query: 72 IRDTSPVERPHVVGVFHEALAKTVNVTSGILEHSTVLVNSAKSPAELKELLKMKAGTIAV 131 +R V P V V E L V+V SG+ + V++N + + G + V Sbjct: 58 VRRRYQVYNPDYVVVLDEGLVDVVDVFSGLKDDGVVVLNKSGD---------FQGGDVKV 108 Query: 132 --IDATSIALKEKNR--VNMAMLGALFRLCPFLDTETMKGVIEKS----LGKKYPQAVQS 183 IDAT IAL+ R VN MLGA + + +++ +I+++ +G+K +A + Sbjct: 109 HTIDATGIALETLGRPIVNTVMLGAFAGVTGLVSIDSLIKIIKETFPGKIGEKNAEAARM 168 Query: 184 AISTFERGYNEVEFMQFELAAGDSMPEYVRSDIGVLGYDTQPMGGSIINPGSTFLKNLSI 243 A Y E+E ++ + + +G ++ PGST +KN + Sbjct: 169 A-------YEEIEKLRVNM--------------------MESLGATVKKPGST-VKNKTG 200 Query: 244 SRSGMLPAFDLEACIHCAQCDTVCPDQCFVWEERVDRKGRSQMYLTGIDYQYCKGCLKCV 303 S P D + CI C C CP+ C E IDY YCKGC C Sbjct: 201 SWRTFKPVLDKDKCIDCDNCILFCPEGCINREHE-------------IDYDYCKGCGICA 247 Query: 304 GACPTSALSSQREK 317 CP A+ +REK Sbjct: 248 EKCPVKAIKMEREK 261 >ref|NP_227831.1| pyruvate ferredoxin oxidoreductase, gamma subunit [Thermotoga maritima] sp|O05650|PORC_THEMA Pyruvate synthase subunit PORC (Pyruvate oxidoreductase gamma chain) (POR) (Pyruvic-ferredoxin oxidoreductase gamma subunit) pir||A72427 pyruvate synthase (EC 1.2.7.1) gamma chain [validated] - Thermotoga maritima (strain MSB8) gb|AAD35109.1| pyruvate ferredoxin oxidoreductase, gamma subunit [Thermotoga maritima] Length = 192 Score = 99.0 bits (245), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 76/191 (39%), Positives = 99/191 (51%), Gaps = 14/191 (7%) Query: 12 FFEIRLESIGGLGANLAGKMLAEAGVVGAGLNGVSFSSYGSEKKGSAVKAHIRFCDLNTP 71 +FEIR G GA A +MLAEA + AG +F YG+E+ G+ ++A R D Sbjct: 7 YFEIRWHGRAGQGAKSASQMLAEAAL-EAGKYVQAFPEYGAERTGAPMRAFNRIGD--EY 63 Query: 72 IRDTSPVERPHVVGVFHEALAKTVNVTSGILEHSTVLVNSAKSPAELKELLKMKAG---T 128 IR S VE P VV V E L + G+ E +LVN+ K E ++ K G Sbjct: 64 IRVRSAVENPDVVVVIDETLLSPA-IVEGLSEDGILLVNTVKD----FEFVRKKTGFNGK 118 Query: 129 IAVIDATSIALKEKNR--VNMAMLGALFRLCPFLDTETMKGVIEKSLGKKYPQAVQSA-I 185 I V+DAT IAL+E R N MLGAL R+ + E ++ IEK GKK+PQ V A Sbjct: 119 ICVVDATDIALQEIKRGIPNTPMLGALVRVTGIVPLEAIEKRIEKMFGKKFPQEVIDANK 178 Query: 186 STFERGYNEVE 196 RGY EV+ Sbjct: 179 RALRRGYEEVK 189 >emb|CAA59455.1| gamma subunit of pyruvate ferredoxin oxidoreductase [Thermotoga maritima] Length = 213 Score = 97.8 bits (242), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 76/200 (38%), Positives = 102/200 (51%), Gaps = 16/200 (8%) Query: 3 QLPIVNDLGFFEIRLESIGGLGANLAGKMLAEAGVVGAGLNGVSFSSYGSEKKGSAVKAH 62 ++P+ +FEIR G GA +MLAEA + AG +F YG+E+ G+ ++A Sbjct: 21 RMPVAKK--YFEIRWHGRAGQGAKSVSQMLAEAAL-EAGKYVQAFPEYGAERTGAPMRAF 77 Query: 63 IRFCDLNTPIRDTSPVERPHVVGVFHEALAKTVNVTSGILEHSTVLVNSAKSPAELKELL 122 R D IR S VE P VV V E L + G+ E +LVN+ K E + Sbjct: 78 NRIGD--EYIRVRSAVENPDVVVVIDETLLSPA-IVEGLSEDGILLVNTVKD----FEFV 130 Query: 123 KMKAG---TIAVIDATSIALKEKNR--VNMAMLGALFRLCPFLDTETMKGVIEKSLGKKY 177 + K G I V+DAT IAL+E R N MLGAL R+ + E ++ IEK GKK+ Sbjct: 131 RKKTGFNGKICVVDATDIALQEIKRGIPNTPMLGALVRVTGIVPLEAIEKRIEKMFGKKF 190 Query: 178 PQAVQSA-ISTFERGYNEVE 196 PQ V A RGY EV+ Sbjct: 191 PQEVIDANKRALRRGYEEVK 210 >ref|ZP_00059749.1| COG1014: Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:ferredoxin oxidoreductases, gamma subunit [Clostridium thermocellum ATCC 27405] Length = 192 Score = 88.6 bits (218), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 61/189 (32%), Positives = 95/189 (50%), Gaps = 10/189 (5%) Query: 14 EIRLESIGGLGANLAGKMLAEAGV-VGAGLNGVSFSSYGSEKKGSAVKAHIRFCDLNTPI 72 EIR GG GA A +LA+A G + G F YG E+ G+ + A+ R D I Sbjct: 6 EIRWHGRGGQGAKTASLLLADAAFNTGKYIQG--FPEYGPERMGAPITAYNRISDEKLTI 63 Query: 73 RDTSPVERPHVVGVFHEALAKTVNVTSGILEHSTVLVNSAKSPAELKELLKMKAGTIAVI 132 S + P V V + L +V+VT+G+ E ++VN+ K+P E++ LLK G + I Sbjct: 64 H--SNIYEPDYVVVVDDTLLTSVDVTAGLKEDGAIIVNTPKTPDEIRPLLKGYKGKVCTI 121 Query: 133 DATSIALKEKNRV--NMAMLGALFRLCPFLDTETMKGVIEKSLGKKY---PQAVQSAIST 187 DA I+++ + N MLGA+ ++ +D E + +S K+ P+ V+ I Sbjct: 122 DARKISIETLGKYFPNTPMLGAVVKVSKIMDEEEFLKDMVESFKHKFANKPEVVEGNIKA 181 Query: 188 FERGYNEVE 196 ER EV+ Sbjct: 182 LERSMQEVK 190 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 471,430,229 Number of extensions: 18933449 Number of successful extensions: 61361 Number of sequences better than 1.0e-05: 79 Number of HSP's gapped: 61612 Number of HSP's successfully gapped: 79 Length of query: 336 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 210 Effective length of database: 415,135,104 Effective search space: 87178371840 Effective search space used: 87178371840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)