BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0026.p (120 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_953847.1| dinitrogenase iron-molybdenum cofactor family p... 60 2e-08 ref|ZP_00082049.1| COG0535: Predicted Fe-S oxidoreductases [Geob... 56 3e-07 ref|NP_616160.1| conserved hypothetical protein [Methanosarcina ... 55 5e-07 sp|P46044|NIFB_FRAAL FeMo cofactor biosynthesis protein nifB >gi... 52 4e-06 ref|NP_662424.1| NifB protein [Chlorobium tepidum TLS] >gi|21647... 52 4e-06 >ref|NP_953847.1| dinitrogenase iron-molybdenum cofactor family protein [Geobacter sulfurreducens PCA] gb|AAR36197.1| dinitrogenase iron-molybdenum cofactor family protein [Geobacter sulfurreducens PCA] Length = 109 Score = 59.7 bits (143), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 40/108 (37%), Positives = 54/108 (50%), Gaps = 4/108 (3%) Query: 7 IAVGSADGYAINEHFGRCGRFLIYEIEQDGTYSQIENRSCE-WRRGQDGHD--VNHLQTA 63 IAV S DG IN+HFG RFLIYE++ G ++ R E + G+ H + L + Sbjct: 3 IAVTSRDGREINQHFGHAERFLIYEVD-GGDARLVDERKIERYCTGESDHSQRAHILSSI 61 Query: 64 ATVLADCAFVLVGQIGPGARAVLYDNGIQAMAVQSPIELALARLALFL 111 A L C ++ QIGPG L G++A PI+ L LA L Sbjct: 62 AGALVGCRALVCAQIGPGPLMELERLGLEAFTADGPIKATLVELAKVL 109 >ref|ZP_00082049.1| COG0535: Predicted Fe-S oxidoreductases [Geobacter metallireducens] Length = 126 Score = 55.8 bits (133), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 43/121 (35%), Positives = 53/121 (43%), Gaps = 20/121 (16%) Query: 4 SWR----IAVGSADGYAINEHFGRCGRFLIYEIEQDGTYSQIEN---RSCEWRRGQDGHD 56 WR IAV S DG IN+HFG RFLIYE+E D E R C + D Sbjct: 13 KWRFPMLIAVASKDGREINQHFGHAERFLIYEVEGDDAKLVDEKKVERYCSY-------D 65 Query: 57 VNH------LQTAATVLADCAFVLVGQIGPGARAVLYDNGIQAMAVQSPIELALARLALF 110 V+H L+ A LA C V+ QIG + + GI A I+ L +A Sbjct: 66 VDHPLRSHILEAIACALAGCRAVVCAQIGQAPQMAMERLGIDAYVADGLIKPTLVEIARV 125 Query: 111 L 111 L Sbjct: 126 L 126 >ref|NP_616160.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] gb|AAM04640.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] Length = 185 Score = 55.1 bits (131), Expect = 5e-07, Method: Compositional matrix adjust. Identities = 37/110 (33%), Positives = 59/110 (53%), Gaps = 6/110 (5%) Query: 4 SWRIAVGSADGYAINEHFGRCGRFLIYEIE-QDGTYSQIENRSC-EWRRGQDGHDVNHLQ 61 +++AV S G +++HFG+ F+I+EIE QD + +E RS ++ G G V L+ Sbjct: 68 KFKVAVTSRGGKLVDQHFGKATTFMIFEIEGQDCKF--LETRSTKKYCNGGSGIGVEPLE 125 Query: 62 TAATV--LADCAFVLVGQIGPGARAVLYDNGIQAMAVQSPIELALARLAL 109 T+ ++DC VL +IG GA+ L + GI + IE L L + Sbjct: 126 KNETIKSISDCDAVLTMKIGSGAQKKLQEYGIDCVEYCYTIECGLKYLQM 175 >sp|P46044|NIFB_FRAAL FeMo cofactor biosynthesis protein nifB pir||JC4208 nitrogenase NifB chain - Frankia sp pir||T09237 nifB protein - Frankia alni gb|AAC82975.1| NifB [Frankia alni] Length = 510 Score = 52.0 bits (123), Expect = 4e-06, Method: Composition-based stats. Identities = 35/114 (30%), Positives = 56/114 (49%), Gaps = 8/114 (7%) Query: 7 IAVGSADGYAINEHFGRCGRFLIYEIEQDGTYSQIENRSCEWRRGQDG-----HDVNHLQ 61 +AV + +N+HFG G F IYE G ++ R + R +G D + L Sbjct: 392 VAVATKGSGVVNQHFGHAGEFWIYE-AGPGWARLVQTRDVD--RYCNGPSDCDEDASKLD 448 Query: 62 TAATVLADCAFVLVGQIGPGARAVLYDNGIQAMAVQSPIELALARLALFLISGR 115 +L+DCA VL +IG G R L + GI+ + + I+ A+A + L++ R Sbjct: 449 RTIEMLSDCAAVLCSKIGLGPREALEEAGIEPVEIYDLIDKAVAEIGARLVTDR 502 >ref|NP_662424.1| NifB protein [Chlorobium tepidum TLS] gb|AAM72766.1| NifB protein [Chlorobium tepidum TLS] Length = 424 Score = 52.0 bits (123), Expect = 4e-06, Method: Composition-based stats. Identities = 33/102 (32%), Positives = 50/102 (49%), Gaps = 11/102 (10%) Query: 7 IAVGSADGYAINEHFGRCGRFLIYEIEQDGTYSQIENRSCEWRRGQD----GHDVNHLQT 62 IAV S +G IN+H G RFL+Y +++ E +SC + G + Sbjct: 297 IAVASLEGVLINQHLGEADRFLVYALDE-------EKKSCTLVDSRQAPPPGGGKLRWEA 349 Query: 63 AATVLADCAFVLVGQIGPGARAVLYDNGIQAMAVQSPIELAL 104 A L+DC VLV G ++VL +GI M+++ IE A+ Sbjct: 350 LAAKLSDCRAVLVNSAGDSPQSVLKASGIDVMSIEGVIEEAV 391 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.324 0.138 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 170,101,899 Number of extensions: 5986402 Number of successful extensions: 13711 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 13738 Number of HSP's successfully gapped: 6 Length of query: 120 Length of database: 757,237,578 Length adjustment: 84 Effective length of query: 36 Effective length of database: 529,169,262 Effective search space: 19050093432 Effective search space used: 19050093432 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 120 (50.8 bits)