BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0024.p (280 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAC27790.1| NifB2 protein [Paenibacillus durus] 260 2e-68 ref|ZP_00112314.1| COG0535: Predicted Fe-S oxidoreductases [Nost... 258 1e-67 ref|ZP_00160867.1| hypothetical protein Avar237001 [Anabaena var... 255 7e-67 ref|NP_485557.1| nitrogen fixation protein [Nostoc sp. PCC 7120]... 251 1e-65 sp|Q43883|NIFB_ANAAZ FeMo cofactor biosynthesis protein nifB >gi... 251 1e-65 >emb|CAC27790.1| NifB2 protein [Paenibacillus durus] Length = 458 Score = 260 bits (665), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 133/251 (52%), Positives = 172/251 (68%) Query: 8 SAMQDIHRHPCYDELAHEVFARMHVAVAPXXXXXXXXXXXXXXXVSESRPGVVSRVLSPG 67 +A DI RHPCY E AH FARMH+ VAP V+ESRPGVVS VL+P Sbjct: 12 AAEDDISRHPCYSEEAHRFFARMHIPVAPACNIQCNYCNRKFDCVNESRPGVVSEVLTPE 71 Query: 68 EAYRQVQDTLKVLPQLTVVGIAGPGDPLANPVQTFETFRLLSREMPDLQLCLSTNGLKLP 127 +A R+V+ L QL+VVGIAGPGDPLAN +TF+TF + + +PD+ CLSTNGL L Sbjct: 72 QAERKVKGVAAQLMQLSVVGIAGPGDPLANADKTFDTFARVKKHVPDVITCLSTNGLTLY 131 Query: 128 DYLEEIKECGIRHVTVTMNAVDPVIGGLIYHSIYYRGKAYRGKDAAEILISRQLEGIRAV 187 +++ I E GI HVT+T+NA+DP +G IY + G Y G++AA +LISRQL+G+ + Sbjct: 132 RHIDRIVELGIGHVTITINAIDPDVGKEIYPWVSDEGVRYEGREAAALLISRQLQGVEEL 191 Query: 188 AALGIKVKVNSVLIPGINDGHLLEVSRVVKAAGAFSHNIMPLIISPGSRFERDGRQPPDP 247 A GI VKVNS+LIPGIND HL+EVS+ VK GA HN+ PLII+PGS++E+DGR+ P P Sbjct: 192 AKRGILVKVNSILIPGINDKHLVEVSKKVKELGATLHNVTPLIIAPGSQYEKDGRKAPRP 251 Query: 248 ELTLKVQEDSS 258 ++QE S Sbjct: 252 RELNQLQEQLS 262 >ref|ZP_00112314.1| COG0535: Predicted Fe-S oxidoreductases [Nostoc punctiforme] Length = 495 Score = 258 bits (658), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 131/248 (52%), Positives = 171/248 (68%), Gaps = 2/248 (0%) Query: 13 IHRHPCYDELAHEVFARMHVAVAPXXXXXXXXXXXXXXXVSESRPGVVSRVLSPGEAYRQ 72 I +HPCY E AH +ARMHVAVAP +ESRPGVVS +L+P EA + Sbjct: 57 IAKHPCYSEDAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSELLTPEEAAHK 116 Query: 73 VQDTLKVLPQLTVVGIAGPGDPLANPVQTFETFRLLSREMPDLQLCLSTNGLKLPDYLEE 132 +PQ+TVVGIAGPGDPLANP +TF TF L++ + PD++LCLSTNGL LPDY++ Sbjct: 117 ALVIGGKIPQMTVVGIAGPGDPLANPEKTFRTFELIAAQAPDIKLCLSTNGLMLPDYIDR 176 Query: 133 IKECGIRHVTVTMNAVDPVIGGLIYHSIYYRGKAYRGKDAAEILISRQLEGIRAVAALGI 192 IK+ I HVT+T+N VDP IG IY ++YR K YRG + A+IL RQ+EG++A+ I Sbjct: 177 IKQLNIDHVTITINMVDPEIGAKIYPWVHYRRKRYRGLEGAKILHERQMEGLQALKDADI 236 Query: 193 KVKVNSVLIPGINDGHLLEVSRVVKAAGAFSHNIMPLIISP--GSRFERDGRQPPDPELT 250 KVNSV+IPGIND HL+EV+RV++ GAF HNIMPLI +P G+ F G++ P P+ Sbjct: 237 LCKVNSVMIPGINDHHLVEVNRVIREKGAFLHNIMPLISAPEHGTHFGLIGQRGPTPKEL 296 Query: 251 LKVQEDSS 258 +VQ++ S Sbjct: 297 KEVQDNCS 304 >ref|ZP_00160867.1| hypothetical protein Avar237001 [Anabaena variabilis ATCC 29413] gb|AAA93017.1| NifB2 Length = 484 Score = 255 bits (652), Expect = 7e-67, Method: Compositional matrix adjust. Identities = 126/248 (50%), Positives = 173/248 (69%), Gaps = 2/248 (0%) Query: 13 IHRHPCYDELAHEVFARMHVAVAPXXXXXXXXXXXXXXXVSESRPGVVSRVLSPGEAYRQ 72 I +HPCY E AH +ARMHVAVAP +ESRPGVVS +L+P EA + Sbjct: 45 IEKHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSELLTPEEAAHK 104 Query: 73 VQDTLKVLPQLTVVGIAGPGDPLANPVQTFETFRLLSREMPDLQLCLSTNGLKLPDYLEE 132 V +PQ+TV+GIAGPGDPLANP +TF TF L++ + PD++LCLSTNGL LPDY++ Sbjct: 105 VLVIAGKIPQMTVLGIAGPGDPLANPEKTFRTFELIAEKAPDIKLCLSTNGLMLPDYVDR 164 Query: 133 IKECGIRHVTVTMNAVDPVIGGLIYHSIYYRGKAYRGKDAAEILISRQLEGIRAVAALGI 192 IK+ I HVT+T+N VDP IG IY ++YR K Y+G +AA+IL +Q+EG++A+ I Sbjct: 165 IKQLNIDHVTITINMVDPEIGTKIYPWVHYRRKRYKGIEAAQILHEKQMEGLQALQEADI 224 Query: 193 KVKVNSVLIPGINDGHLLEVSRVVKAAGAFSHNIMPLIISP--GSRFERDGRQPPDPELT 250 KVNSV+IPGIND HL+EV++V+++ GAF HNIMPLI +P G+ F G++ P + Sbjct: 225 LCKVNSVMIPGINDEHLVEVNQVIRSKGAFLHNIMPLISAPEHGTHFGLTGQRGPTAKEL 284 Query: 251 LKVQEDSS 258 ++Q++ + Sbjct: 285 KQIQDNCA 292 >ref|NP_485557.1| nitrogen fixation protein [Nostoc sp. PCC 7120] sp|P20627|NIFB_ANASP FeMo cofactor biosynthesis protein nifB pir||A34443 nitrogen fixation protein nifB - Anabaena sp pir||AG1995 nitrogen fixation protein [imported] - Nostoc sp. (strain PCC 7120) gb|AAA22004.1| nitrogenase [Nostoc sp. PCC 7120] dbj|BAB77883.1| nitrogen fixation protein [Nostoc sp. PCC 7120] Length = 475 Score = 251 bits (642), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 123/248 (49%), Positives = 169/248 (68%), Gaps = 2/248 (0%) Query: 13 IHRHPCYDELAHEVFARMHVAVAPXXXXXXXXXXXXXXXVSESRPGVVSRVLSPGEAYRQ 72 I +HPCY E AH +ARMHVAVAP +ESRPGVVS +L+P EA + Sbjct: 41 IAKHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSELLTPEEAAHK 100 Query: 73 VQDTLKVLPQLTVVGIAGPGDPLANPVQTFETFRLLSREMPDLQLCLSTNGLKLPDYLEE 132 V +PQ+TV+GIAGPGDPLANP +TF TF L++ + PD++LCLSTNGL LP+Y++ Sbjct: 101 VLVIAGKIPQMTVLGIAGPGDPLANPEKTFRTFELIADKAPDIKLCLSTNGLMLPEYVDR 160 Query: 133 IKECGIRHVTVTMNAVDPVIGGLIYHSIYYRGKAYRGKDAAEILISRQLEGIRAVAALGI 192 IK+ I HVT+T+N +DP IG IY ++Y+ + YRG + A IL+ +Q+EG++A+ I Sbjct: 161 IKQLNIDHVTITLNTIDPEIGAQIYSWVHYKRRRYRGAEGARILLEKQMEGLQALREADI 220 Query: 193 KVKVNSVLIPGINDGHLLEVSRVVKAAGAFSHNIMPLIISP--GSRFERDGRQPPDPELT 250 KVNSV+IPGIND HL+EV+++++ GAF HNIMPLI +P G+ F G++ P + Sbjct: 221 LCKVNSVMIPGINDQHLVEVNKMIREQGAFLHNIMPLISAPEHGTHFGLTGQRGPSQKEL 280 Query: 251 LKVQEDSS 258 VQ+ S Sbjct: 281 KSVQDQCS 288 >sp|Q43883|NIFB_ANAAZ FeMo cofactor biosynthesis protein nifB gb|AAA87247.1| NifB protein Length = 475 Score = 251 bits (641), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 123/248 (49%), Positives = 169/248 (68%), Gaps = 2/248 (0%) Query: 13 IHRHPCYDELAHEVFARMHVAVAPXXXXXXXXXXXXXXXVSESRPGVVSRVLSPGEAYRQ 72 I +HPCY E AH +ARMHVAVAP +ESRPGVVS +L+P EA + Sbjct: 41 IAKHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSELLTPEEAAHK 100 Query: 73 VQDTLKVLPQLTVVGIAGPGDPLANPVQTFETFRLLSREMPDLQLCLSTNGLKLPDYLEE 132 V +PQ+TV+GIAGPGDPLANP +TF TF L++ + PD++LCLSTNGL LP+Y++ Sbjct: 101 VLVIAGKIPQMTVLGIAGPGDPLANPEKTFRTFELIADKAPDIKLCLSTNGLMLPEYVDR 160 Query: 133 IKECGIRHVTVTMNAVDPVIGGLIYHSIYYRGKAYRGKDAAEILISRQLEGIRAVAALGI 192 IK+ I HVT+T+N +DP IG IY ++Y+ + YRG + A IL+ +Q+EG++A+ I Sbjct: 161 IKQLNIDHVTITLNTIDPEIGAQIYSWVHYKRRRYRGAEGARILLEKQMEGLQALREADI 220 Query: 193 KVKVNSVLIPGINDGHLLEVSRVVKAAGAFSHNIMPLIISP--GSRFERDGRQPPDPELT 250 KVNSV+IPGIND HL+EV+++++ GAF HNIMPLI +P G+ F G++ P + Sbjct: 221 LCKVNSVMIPGINDQHLVEVNKMIREQGAFLHNIMPLISAPEHGTHFGLTGQRGPSQKEL 280 Query: 251 LKVQEDSS 258 VQ+ S Sbjct: 281 KSVQDQCS 288 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.139 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 384,495,639 Number of extensions: 14503583 Number of successful extensions: 31697 Number of sequences better than 1.0e-05: 76 Number of HSP's gapped: 31766 Number of HSP's successfully gapped: 76 Length of query: 280 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 156 Effective length of database: 420,565,302 Effective search space: 65608187112 Effective search space used: 65608187112 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)