BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0023.p (162 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAD56228.1| NifB1 protein [Paenibacillus durus] 122 2e-27 emb|CAC27790.1| NifB2 protein [Paenibacillus durus] 122 3e-27 gb|AAD17269.1| NifB [Frankia sp. EuIK1] 100 8e-21 sp|P46044|NIFB_FRAAL FeMo cofactor biosynthesis protein nifB >gi... 94 8e-19 ref|ZP_00160867.1| hypothetical protein Avar237001 [Anabaena var... 94 1e-18 >emb|CAD56228.1| NifB1 protein [Paenibacillus durus] Length = 320 Score = 122 bits (307), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 67/158 (42%), Positives = 99/158 (62%), Gaps = 10/158 (6%) Query: 3 ALPVYTLEERENELQKLDNELQVRRDRRQN--GTAPGSGGIRIAVASRGGGKVNQHFGHA 60 A PV L+ RE Q+LD +++ R ++N + R+AVASRGG KVNQHFGHA Sbjct: 160 ADPVIDLDAREQFQQELDIKIRQRLIAKENRRTILDENKKTRVAVASRGGDKVNQHFGHA 219 Query: 61 REFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGAGILKETVEMLRDCRLLLCAGIG 120 EF++++ + +G+R+IQAYC+GTA C G++ L+E +L DCR+LL +GIG Sbjct: 220 TEFMIFDTDGTEVKFIGIRKIQAYCHGTADCNGDKDET--LQEITSILSDCRILLSSGIG 277 Query: 121 PVPQEVLHRAEITVIVTKDAIESSL---VKYGRLLSYF 155 P+ VL++A + +V K I+ ++ VKYG SYF Sbjct: 278 DAPRAVLNKAGVLPLVRKGGIQEAILESVKYG---SYF 312 >emb|CAC27790.1| NifB2 protein [Paenibacillus durus] Length = 458 Score = 122 bits (306), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 66/158 (41%), Positives = 99/158 (62%), Gaps = 10/158 (6%) Query: 3 ALPVYTLEERENELQKLDNELQVRRDRRQN--GTAPGSGGIRIAVASRGGGKVNQHFGHA 60 A PV L+ RE Q+LD++++ R ++N + R+AVASRGG KVNQHFGHA Sbjct: 298 ADPVIDLDAREQFQQELDSKIRQRLIAKENRRSILDENKKTRVAVASRGGDKVNQHFGHA 357 Query: 61 REFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGAGILKETVEMLRDCRLLLCAGIG 120 EF++++ + +G+R+IQAYC+GTA C G++ L+E +L DCR+LL +GIG Sbjct: 358 TEFMIFDTDGAEVKFIGIRKIQAYCHGTADCNGDK--GETLQEITSILSDCRILLSSGIG 415 Query: 121 PVPQEVLHRAEITVIVTKDAIESSL---VKYGRLLSYF 155 P+ L++A + +V K I+ ++ VKYG SYF Sbjct: 416 DAPRAALNKAGVLPLVRKGGIQEAILESVKYG---SYF 450 >gb|AAD17269.1| NifB [Frankia sp. EuIK1] Length = 658 Score = 100 bits (250), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 57/152 (37%), Positives = 80/152 (52%), Gaps = 8/152 (5%) Query: 7 YTLEERENELQKLDNELQVRRDRRQNGTAPGSGG-----IRIAVASRGGGKVNQHFGHAR 61 Y LE R+ +++ Q R R+ T G + +AVA++G G VNQHFGHA Sbjct: 339 YDLEGRQAAHPEIERWRQETRAERETVTVAGPATKPDEVVLVAVATKGSGVVNQHFGHAT 398 Query: 62 EFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGAGILKETVEMLRDCRLLLCAGIGP 121 EF +YE ARL+ R +Q YC G C GA +L +T+EML DC +LC+ IGP Sbjct: 399 EFWIYEAGRNWARLVQTRDVQRYCEGPTECG---DGASVLDKTIEMLSDCSAVLCSKIGP 455 Query: 122 VPQEVLHRAEITVIVTKDAIESSLVKYGRLLS 153 P + L A I + + IE ++ G L+ Sbjct: 456 GPYDALEAAGIEPVEVYELIEKAVAAAGARLT 487 >sp|P46044|NIFB_FRAAL FeMo cofactor biosynthesis protein nifB pir||JC4208 nitrogenase NifB chain - Frankia sp pir||T09237 nifB protein - Frankia alni gb|AAC82975.1| NifB [Frankia alni] Length = 510 Score = 94.4 bits (233), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 50/121 (41%), Positives = 71/121 (58%), Gaps = 4/121 (3%) Query: 41 IRIAVASRGGGKVNQHFGHAREFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGAGI 100 + +AVA++G G VNQHFGHA EF +YE ARL+ R + YCNG + C ++ A Sbjct: 390 VLVAVATKGSGVVNQHFGHAGEFWIYEAGPGWARLVQTRDVDRYCNGPSDC---DEDASK 446 Query: 101 LKETVEMLRDCRLLLCAGIGPVPQEVLHRAEITVIVTKDAIESSLVKYG-RLLSYFTPGA 159 L T+EML DC +LC+ IG P+E L A I + D I+ ++ + G RL++ P Sbjct: 447 LDRTIEMLSDCAAVLCSKIGLGPREALEEAGIEPVEIYDLIDKAVAEIGARLVTDRAPAE 506 Query: 160 V 160 V Sbjct: 507 V 507 >ref|ZP_00160867.1| hypothetical protein Avar237001 [Anabaena variabilis ATCC 29413] gb|AAA93017.1| NifB2 Length = 484 Score = 93.6 bits (231), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 54/151 (35%), Positives = 84/151 (55%), Gaps = 13/151 (8%) Query: 5 PVYTLEERENE---LQKLDNELQVRRDR----RQNGTAPGSGGIRIAVASRGGGKVNQHF 57 P Y LE+R+ ++K E+QV +++ Q + S I +AVA++GGG VNQHF Sbjct: 330 PEYNLEQRQTVHAGIEKSQKEIQVVKEKVVETLQTTSFNNSPKILVAVATKGGGLVNQHF 389 Query: 58 GHAREFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGAGILKETVEMLRDCRLLLCA 117 GHA+EF++YEV +SA+ + R+I YC GEE L + + DC+ +L + Sbjct: 390 GHAKEFMIYEVDGKSAKFVSHRKIDHYCQSG---YGEE---ATLDNIIHAISDCQAVLVS 443 Query: 118 GIGPVPQEVLHRAEITVIVTKDAIESSLVKY 148 IG PQE L +A + + D IE +++ Sbjct: 444 KIGNCPQEQLLKAGLQTVEAYDVIEKVALEF 474 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 232,741,361 Number of extensions: 8825926 Number of successful extensions: 23620 Number of sequences better than 1.0e-05: 51 Number of HSP's gapped: 23598 Number of HSP's successfully gapped: 51 Length of query: 162 Length of database: 757,237,578 Length adjustment: 116 Effective length of query: 46 Effective length of database: 442,286,094 Effective search space: 20345160324 Effective search space used: 20345160324 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 121 (51.2 bits)