BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0022.p (342 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060673.1| COG2873: O-acetylhomoserine sulfhydrylase [Cl... 393 e-108 ref|NP_228690.1| O-acetylhomoserine sulfhydrylase [Thermotoga ma... 301 1e-80 ref|ZP_00082400.1| COG2873: O-acetylhomoserine sulfhydrylase [Ge... 284 3e-75 ref|NP_952236.1| O-acetyl-L-homoserine sulfhydrylase [Geobacter ... 282 8e-75 ref|NP_962391.1| MetC [Mycobacterium avium subsp. paratuberculos... 278 1e-73 >ref|ZP_00060673.1| COG2873: O-acetylhomoserine sulfhydrylase [Clostridium thermocellum ATCC 27405] Length = 451 Score = 393 bits (1010), Expect = e-108, Method: Compositional matrix adjust. Identities = 185/326 (56%), Positives = 237/326 (72%), Gaps = 1/326 (0%) Query: 10 FDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTRIGNPTVAV 69 FDT ++RAGYD +EHNYAV+ PIYQT S+D VE + LFG+ E G LYTRIGNPTVAV Sbjct: 25 FDTQRLRAGYDPKEHNYAVSPPIYQTTSFDFRDVEHAKALFGLRELGNLYTRIGNPTVAV 84 Query: 70 LEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDALKHLYPKFG 129 LEQR+ ALD +Y LLN+AEGGGRILT+P LYGG+ D+ K +YPKFG Sbjct: 85 LEQRVAALDGASGAVALASGMAAISYTLLNVAEGGGRILTSPYLYGGSADSFKKVYPKFG 144 Query: 130 VHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDNGIPLVIDNT 189 + DF ++ ++PE +I PDTKAI VESISNPN+ +LDI+AIA+ AH++GIPL++DNT Sbjct: 145 ITFDFAKNIENPEKLSEEIKPDTKAIYVESISNPNAALLDIDAIAKVAHEHGIPLIVDNT 204 Query: 190 FGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGRFPHFEEPQYLLR 249 TPYL++ +HGADIV+YSATK + GHG + G++LE+G FNW + +FP F E Y LR Sbjct: 205 VATPYLYNPISHGADIVVYSATKGLTGHGNVIAGLVLESGKFNWQSDKFPQFSEKYYTLR 264 Query: 250 EAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLSERISKQVAN 309 + RS LEVFP+APFT R+R NYL YFGA L PFDA+L+L G+ETLSER+ KQV N Sbjct: 265 DINDNY-RSFLEVFPEAPFTGRIRFNYLNYFGAALSPFDAYLVLIGLETLSERVEKQVRN 323 Query: 310 ALRIVDYLQKHDQVSWVSHPVAEGNP 335 A + +YL+ H V WV +P + +P Sbjct: 324 ASILAEYLKSHKSVEWVRYPGLKDSP 349 >ref|NP_228690.1| O-acetylhomoserine sulfhydrylase [Thermotoga maritima] pir||D72324 O-acetylhomoserine sulfhydrylase - Thermotoga maritima (strain MSB8) gb|AAD35963.1| O-acetylhomoserine sulfhydrylase [Thermotoga maritima] Length = 430 Score = 301 bits (771), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 156/334 (46%), Positives = 215/334 (64%), Gaps = 10/334 (2%) Query: 3 MTDKKLGFDTLKVRAGYDSREHNY-AVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTR 61 M KK G++T + AGY+ E + AVPIYQT SY + +LF +EEPG++YTR Sbjct: 1 MDWKKYGYNTRALHAGYEPPEQATGSRAVPIYQTTSYVFRDSDHAARLFALEEPGFIYTR 60 Query: 62 IGNPTVAVLEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDAL 121 IGNPTV+VLE+R+ AL++ TYA+LN+A G I++ LYGGT++ Sbjct: 61 IGNPTVSVLEERIAALEEGVGALAVASGQAAITYAILNIAGPGDEIVSGSALYGGTYNLF 120 Query: 122 KH-LYPKFGVHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDN 180 +H LY K G+ V FV D DP+ I TKA+ +E+I NP TV D EAIAE AH + Sbjct: 121 RHTLYKKSGIIVKFV-DETDPKNIEEAITEKTKAVYLETIGNPGLTVPDFEAIAEIAHRH 179 Query: 181 GIPLVIDNTFGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGRFPH 240 G+PL++DNT PY+F F HGADIV+YSATK IGGHGT++GG+I+++G F+W NG+FP Sbjct: 180 GVPLIVDNTVA-PYIFRPFEHGADIVVYSATKFIGGHGTSIGGLIVDSGKFDWTNGKFPE 238 Query: 241 FEEPQYLLREAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLS 300 EP + S +E F +A + A+ R L G+ + PF+AFL + G+ETLS Sbjct: 239 LVEPD------PSYHGVSYVETFKEAAYIAKCRTQLLRDLGSCMSPFNAFLFILGLETLS 292 Query: 301 ERISKQVANALRIVDYLQKHDQVSWVSHPVAEGN 334 R+ K NAL+IV++L+ H VSWV++P+AEGN Sbjct: 293 LRMKKHCENALKIVEFLKSHPAVSWVNYPIAEGN 326 >ref|ZP_00082400.1| COG2873: O-acetylhomoserine sulfhydrylase [Geobacter metallireducens] Length = 428 Score = 284 bits (726), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 146/327 (44%), Positives = 195/327 (59%), Gaps = 7/327 (2%) Query: 3 MTDKKLGFDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTRI 62 MTD K+ FDTL + AG + + AVPIYQTASY S E LFG++E G +YTR+ Sbjct: 1 MTDTKISFDTLALHAGQEPDPTTLSRAVPIYQTASYAFKSSEHAANLFGLKEFGNIYTRL 60 Query: 63 GNPTVAVLEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDALK 122 NPT VLE+RL LD TYA+LN+A+ G I++T LYGGT++ Sbjct: 61 MNPTSDVLEKRLAELDGGVGALAVASGQAAITYAVLNIAQAGQNIVSTSYLYGGTYNLFH 120 Query: 123 HLYPKFGVHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDNGI 182 + PK G+ V FV D+ DPE RR I T+ + ES+ NP + V D EAIA AH+ GI Sbjct: 121 YTLPKLGITVKFV-DTSDPENVRRAIDDKTRLVYTESVGNPKNNVDDFEAIARIAHEAGI 179 Query: 183 PLVIDNTFGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGRFPHFE 242 P V+DNT T YLF HGADIV+YS TK IGGHGT++GG +++ G F W NGRFP Sbjct: 180 PFVVDNTVTTSYLFKPLEHGADIVVYSLTKFIGGHGTSIGGAVVDGGKFPWNNGRFPEMT 239 Query: 243 EPQYLLREAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLSER 302 EP + E + + ++R+ L GA + PF++FL LQG+ETL R Sbjct: 240 EPD------PSYHGLCYWEALGNLSYILKMRITLLRDMGACISPFNSFLFLQGLETLPVR 293 Query: 303 ISKQVANALRIVDYLQKHDQVSWVSHP 329 +++ V NA + +L+KH VSWV++P Sbjct: 294 MARHVENARAVAQWLEKHPLVSWVNYP 320 >ref|NP_952236.1| O-acetyl-L-homoserine sulfhydrylase [Geobacter sulfurreducens PCA] gb|AAR34559.1| O-acetyl-L-homoserine sulfhydrylase [Geobacter sulfurreducens PCA] Length = 428 Score = 282 bits (722), Expect = 8e-75, Method: Compositional matrix adjust. Identities = 144/327 (44%), Positives = 198/327 (60%), Gaps = 7/327 (2%) Query: 3 MTDKKLGFDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTRI 62 M+D + GFDTL + AG D + AVPIYQT+SY S E LFG+ E G +YTRI Sbjct: 1 MSDIQKGFDTLALHAGQDPDPTTLSRAVPIYQTSSYAFRSSEHAANLFGLREFGNIYTRI 60 Query: 63 GNPTVAVLEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDALK 122 NPT VLE+RL LD TYA+LN+A G I++T LYGGT++ Sbjct: 61 MNPTCDVLEKRLAELDGGVGALALASGQAAITYAVLNIAGAGQNIVSTSYLYGGTYNLFH 120 Query: 123 HLYPKFGVHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDNGI 182 + P+ G+ V FV D+ DPE RR + +T+ + ES+ NP + V D E+IA AH+ GI Sbjct: 121 YTLPRLGISVRFV-DTSDPENVRRAMDENTRLVYTESVGNPKNNVDDFESIARIAHEAGI 179 Query: 183 PLVIDNTFGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGRFPHFE 242 P ++DNT TPYLF F HGADI +YS TK IGGHGT++GG ++++G F W NGRFP F Sbjct: 180 PFIVDNTVTTPYLFRPFDHGADIAVYSLTKFIGGHGTSIGGAVVDSGRFPWNNGRFPEFT 239 Query: 243 EPQYLLREAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLSER 302 EP + E + + ++R+ L GA L PF+AFL LQG+ETL R Sbjct: 240 EPD------PSYHGLRYWEALGNLSYILKMRITLLRDMGACLAPFNAFLFLQGLETLPVR 293 Query: 303 ISKQVANALRIVDYLQKHDQVSWVSHP 329 +++ V NA + ++L++H V+WV++P Sbjct: 294 MARHVDNARTVAEWLERHPLVTWVNYP 320 >ref|NP_962391.1| MetC [Mycobacterium avium subsp. paratuberculosis str. k10] gb|AAS06007.1| MetC [Mycobacterium avium subsp. paratuberculosis str. k10] Length = 456 Score = 278 bits (711), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 142/326 (43%), Positives = 196/326 (60%), Gaps = 7/326 (2%) Query: 10 FDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTRIGNPTVAV 69 F+T +V AG A A+PIYQT SY LFG+E PG +YTRIGNPT V Sbjct: 18 FETKQVHAGQHPDSATNARALPIYQTTSYTFDDTTHAAALFGLEVPGNIYTRIGNPTTDV 77 Query: 70 LEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDALKHLYPKFG 129 +EQR+ AL+ T+A+LNLA G I+++PRLYGGT++ + K G Sbjct: 78 VEQRIAALEGGVAALFLSSGQAAETFAILNLASAGDHIVSSPRLYGGTYNLFHYSLAKLG 137 Query: 130 VHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDNGIPLVIDNT 189 + V FVED DD ++++ + P+TKA E+ISNP +LDI ++ AH NG+PL++DNT Sbjct: 138 IEVSFVEDPDDLDSWQAAVRPNTKAFFAETISNPQIDILDIPGVSGVAHANGVPLIVDNT 197 Query: 190 FGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGRFPHFEEPQYLLR 249 TPYL FAHGADIV++SATK +GGHG+ + GVI++ G F+W GRFP F P Sbjct: 198 IATPYLIQPFAHGADIVVHSATKYLGGHGSAIAGVIVDGGTFDWTQGRFPGFTTPD---- 253 Query: 250 EAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLSERISKQVAN 309 + E+ P A + + R+ L G+ PF+AFL+ QGIETLS R+ + VAN Sbjct: 254 --PSYHGVVFAELGPPA-YALKARVQLLRDLGSAAAPFNAFLVAQGIETLSLRMERHVAN 310 Query: 310 ALRIVDYLQKHDQVSWVSHPVAEGNP 335 A R+ ++L HD V V++ G+P Sbjct: 311 AQRVAEFLASHDGVLSVNYAGLPGSP 336 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.139 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 518,088,013 Number of extensions: 21659463 Number of successful extensions: 50082 Number of sequences better than 1.0e-05: 1539 Number of HSP's gapped: 47550 Number of HSP's successfully gapped: 1552 Length of query: 342 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 216 Effective length of database: 415,135,104 Effective search space: 89669182464 Effective search space used: 89669182464 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)