BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0018.p (493 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060669.1| COG2710: Nitrogenase molybdenum-iron protein,... 576 e-163 ref|ZP_00014636.1| COG2710: Nitrogenase molybdenum-iron protein,... 459 e-128 ref|NP_947978.1| possible nitrogenase molybdenum-iron protein al... 457 e-127 ref|ZP_00014614.1| COG2710: Nitrogenase molybdenum-iron protein,... 447 e-124 ref|NP_947693.1| possible nitrogenase molybdenum-iron protein al... 425 e-117 >ref|ZP_00060669.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Clostridium thermocellum ATCC 27405] Length = 491 Score = 576 bits (1485), Expect = e-163, Method: Compositional matrix adjust. Identities = 277/488 (56%), Positives = 347/488 (71%), Gaps = 3/488 (0%) Query: 1 MSKVNLNTPEVPVRELRLGSITGFAGTAAGLLSCSNNGGMKDGSRSFSQCMGCSSGNAFC 60 MSK+NL+ PEV +RE+R+ SITG+ G A L+ + G+KD RSFSQC+GC++ A C Sbjct: 1 MSKINLSLPEVQIREIRINSITGYQGDAKELVE-AREFGLKDKERSFSQCLGCATSKAAC 59 Query: 61 QLSMITDAAMVNHAPVGCAGDFFGYNFVYRVGQMERNLP-PVIGRYFNTNIEEMDTVFGG 119 +I DAA+++H PVGCA + F YRV R + P R F+TN++E DTV+GG Sbjct: 60 MTVLIQDAAVISHGPVGCASCLHEFAFTYRVNYPLRGIERPTPRRIFSTNLKEKDTVYGG 119 Query: 120 ARKLEATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKELGIPVVTCFCEGFKS 179 KL TI+ YER NAIFV T+CA+GIIGDDVESV N+ +ELGIPVV FCEGF+S Sbjct: 120 NIKLANTIREVYERTHANAIFVLTTCAAGIIGDDVESVCNEAEEELGIPVVAIFCEGFRS 179 Query: 180 KIWTSGFDSAYHSIVRKIV-KPPEKKTNKVNIINFWGSDVFSGLLNKLGYEADYIVPFST 238 K+WT+GFD+AYH I RK++ KP ++ + +N+INFWGSDVF G + +YI+PFST Sbjct: 180 KVWTTGFDAAYHGIARKLIQKPRRRRDDMINVINFWGSDVFYEWFAPFGAKPNYIIPFST 239 Query: 239 VAQLERISEAAATIQICPTLGTYLGAALEQVYGVPEIKAPVAYGVAGTDAWMRELGRVLD 298 V L+ SEAAAT+Q C TLG+YLGA LEQ +GVPEI A YG+A TD W R LG++L Sbjct: 240 VNGLKYASEAAATVQACSTLGSYLGAVLEQDFGVPEIPAAPPYGIAQTDRWFRALGKILG 299 Query: 299 RXXXXXXXXXXXXXXVLPQLEAYRAKLAGTTCYITAGSAHGHALIALLRELGINVQGAAI 358 + LP++EA R KLAG T Y+TAG+AHGHAL+ +L ELGI GAAI Sbjct: 300 KEEIAEKIIAEKKKEYLPKIEALREKLAGKTAYVTAGAAHGHALLDVLGELGIKAVGAAI 359 Query: 359 FHHDPVYDNGDPAADMLDHTVKTYGDVPNYNVCNKQAYELANILNRVCPDLMIARHGGMT 418 FHHDP+YD+G D L V YG+V NYNVCNKQ +EL N LNR+ PD+++ARHGGMT Sbjct: 360 FHHDPIYDSGREENDQLAQRVADYGNVFNYNVCNKQEFELVNALNRLRPDVLLARHGGMT 419 Query: 419 HWGAKLGIPTLLIGDEHFSWGYRGLLNYAERILETLDNREFVTNLAKHSTMPYTKWWMEQ 478 WGAKLGIP+LLIGDEH+S GY GL+NY ERILE ++N EFV NL KH+ PYTKWW+EQ Sbjct: 420 LWGAKLGIPSLLIGDEHYSMGYEGLVNYGERILEVIENDEFVKNLEKHAINPYTKWWLEQ 479 Query: 479 NPYTFLGG 486 PY FL G Sbjct: 480 PPYYFLKG 487 >ref|ZP_00014636.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Rhodospirillum rubrum] Length = 487 Score = 459 bits (1182), Expect = e-128, Method: Compositional matrix adjust. Identities = 227/486 (46%), Positives = 309/486 (63%), Gaps = 3/486 (0%) Query: 1 MSKVNLNTPEVPVRELRLGSITGFAGTAAGLLSCSNNGGMKDGSRSFSQCMGCSSGNAFC 60 M+K+ V +RE RLGSITG++GTA L++ S +G + + RSF Q C+ A Sbjct: 1 MTKIEKPLQPVEIRERRLGSITGYSGTAGDLVARSRDGSLANRERSFQQASMCAGLCATL 60 Query: 61 QLSMITDAAMVNHAPVGCAGDFFGYNFVYRVGQMERNLPPVIGRYFNTNIEEMDTVFGGA 120 L++I DA ++NHAPVGCAGDF N R GQ +R + R +T + E DT+FGGA Sbjct: 61 NLTLIEDAVVINHAPVGCAGDFAQLNRCRRRGQGKRAMEVTNARLLSTALTEHDTIFGGA 120 Query: 121 RKLEATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKELGIPVVTCFCEGFKSK 180 +KL TI A R P AIFVT SCASGI+G+D++SV + + IPVV C GF+++ Sbjct: 121 QKLAETIDQAVARYHPRAIFVTASCASGIVGEDIDSVVTEAEERHAIPVVAIDCVGFRTQ 180 Query: 181 IWTSGFDSAYHSIVRKIVKPP-EKKTNKVNIINFWGSDV-FSGLLNKLGYEADYIVPFST 238 +W +GFD AY +++RKIVKP K+ VNI+ F G FS LL LG A+ IV F T Sbjct: 181 VWATGFDMAYDALLRKIVKPAVTKRPEVVNIVCFSGKKAYFSELLEPLGLVANPIVQFLT 240 Query: 239 VAQLERISEAAATIQICPTLGTYLGAALEQVYGVPEIKAPVAYGVAGTDAWMRELGRVLD 298 VA++E +SEA AT +CPTLGT+LGA LE+ +GVPE+KAP YG+AGTD W+R L + Sbjct: 241 VAEIETLSEAGATAHMCPTLGTWLGAGLERRFGVPEVKAPPPYGLAGTDHWLRALAALTG 300 Query: 299 RXXXXXXXXXXXXXXVLPQLEAYRAKLAGTTCYITAGSAHGHALIALLRELGINVQGAAI 358 R + P L RA+LAG T ++ AGSA GH+ + +LR+LGI+V G Sbjct: 301 RSDRVEAYIAAERAAIAPDLADLRAQLAGRTAFVAAGSAQGHSFMTVLRDLGIDVIGGCA 360 Query: 359 FHHDPVYDNGDPAADMLDHTVKTYGDVPNYNVCNKQAYELANILNRVCPDLMIARHGGMT 418 HHDPV D+G D L H + +VP Y VCNKQ++EL N++ ++ PD+++ RH G+ Sbjct: 361 LHHDPVLDHGHQEEDTLAHATRDKAEVP-YGVCNKQSFELINLVGKLKPDILLIRHPGLV 419 Query: 419 HWGAKLGIPTLLIGDEHFSWGYRGLLNYAERILETLDNREFVTNLAKHSTMPYTKWWMEQ 478 WGAKLGIPTL + DEH GYRGL+ Y +I + + N LA+ S +P+T WW++Q Sbjct: 420 VWGAKLGIPTLYVEDEHAGIGYRGLIRYGRKIADWIANPVLERTLARRSPLPFTPWWLDQ 479 Query: 479 NPYTFL 484 +PY FL Sbjct: 480 SPYQFL 485 >ref|NP_947978.1| possible nitrogenase molybdenum-iron protein alpha chain (nitrogenase component I) (dinitrogenase) [Rhodopseudomonas palustris CGA009] emb|CAE28077.1| possible nitrogenase molybdenum-iron protein alpha chain (nitrogenase component I) (dinitrogenase) [Rhodopseudomonas palustris CGA009] Length = 502 Score = 457 bits (1175), Expect = e-127, Method: Compositional matrix adjust. Identities = 224/495 (45%), Positives = 307/495 (62%), Gaps = 15/495 (3%) Query: 4 VNLNTPEVPVRELRLGSITGFAGTAAGL-----LSCSNNGGMKDGSRS-------FSQCM 51 +NL T V RE RLG+I G+ GTA+ L S + GG +R F+Q Sbjct: 3 LNLKTSSVETREQRLGTIIGWDGTASKLADESAFSRGDCGGCGTKARRVCELEGPFTQGS 62 Query: 52 GCSSGNAFCQLSMITDAAMVNHAPVGCAGDFFGYNFVYRVGQMERNLPPVIGRYFNTNIE 111 CS CQ I DA ++ H+P+GC YN +YR G R LP R+ +TN+ Sbjct: 63 VCSEQMVECQAGNIRDAVLIQHSPIGCGAGQVIYNSIYRNGLAMRGLPVQNIRFISTNLR 122 Query: 112 EMDTVFGGARKLEATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKELGIPVVT 171 E D V+GGA KL I+LA ER +P A+F+ TSCASGIIGDD+ESV E GIP++ Sbjct: 123 ERDMVYGGAEKLAEAIRLAVERHQPKAVFIATSCASGIIGDDIESVARTAEAEFGIPIIP 182 Query: 172 CFCEGFKSKIWTSGFDSAYHSIVRKIVK--PPEKKTNKVNIINFWGSDVFSGLLNKLGYE 229 CEGFKSK W++GFD+ H I+R+IV+ P +++ + +N+IN WG+DVFS +L +LG Sbjct: 183 LHCEGFKSKHWSTGFDATQHGILRQIVRRNPDKRQDDLINVINLWGTDVFSPMLGELGLR 242 Query: 230 ADYIVPFSTVAQLERISEAAATIQICPTLGTYLGAALEQVYGVPEIKAPVAYGVAGTDAW 289 +Y+V +TV +L ++SEA AT+ C TLGTYL ALEQ +GV +IKAP YG AGTDAW Sbjct: 243 VNYVVDMATVEELAQMSEARATVSFCYTLGTYLATALEQEFGVEQIKAPQPYGFAGTDAW 302 Query: 290 MRELGRVLDRXXXXXXXXXXXXXXVLPQLEAYRAKLAGTTCYITAGSAHGHALIALLREL 349 +REL RV R V P L+ R KL G Y+ GSA+ H LI +LRE+ Sbjct: 303 LRELARVTGREAQAEDYIAREHARVKPLLDELRPKLKGLKGYVATGSAYSHGLIGVLREI 362 Query: 350 GINVQGAAIFHHDPVYDNGDPAADMLDHTVKTYGDVPNYNVCNKQAYELANILNRVCPDL 409 G+ V G+ +FHHDPVYD+ DP D L H ++ YGDV ++ V N+Q Y+ N+L RV PD Sbjct: 363 GVEVDGSLVFHHDPVYDSEDPRQDSLGHLIENYGDVRHFTVGNRQQYQFYNLLQRVKPDF 422 Query: 410 MIARHGGMTHWGAKLGIPTLLIGDEHFSWGYRGLLNYAERILETLDNREFVTNLAKHSTM 469 +I RH G+ A+LGIP + +GDEH + GY+G++N E IL L +R+F ++AK ++ Sbjct: 423 IIIRHNGLAPLAARLGIPAIPLGDEHHALGYQGMVNLGESILAALQHRKFHKDIAKRVSL 482 Query: 470 PYTKWWMEQ-NPYTF 483 PY + W+ Q +P+ Sbjct: 483 PYKQSWLAQKDPFAL 497 >ref|ZP_00014614.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Rhodospirillum rubrum] Length = 511 Score = 447 bits (1150), Expect = e-124, Method: Compositional matrix adjust. Identities = 220/496 (44%), Positives = 304/496 (61%), Gaps = 16/496 (3%) Query: 4 VNLNTPEVPVRELRLGSITGFAGTAAGLLS---------CSNNGGMK-----DGSRSFSQ 49 +NL T V RE RLG+I + G A+ L C + G K + FSQ Sbjct: 3 INLKTSVVESREQRLGTIIAWDGKASDLSKESAYARSEGCGSACGAKARRVCEMRSPFSQ 62 Query: 50 CMGCSSGNAFCQLSMITDAAMVNHAPVGCAGDFFGYNFVYRVGQMERNLPPVIGRYFNTN 109 CS CQ + A +V H+P+GC YN ++R G R LP +TN Sbjct: 63 GSVCSEQMVECQAGNVRGAVLVQHSPIGCGAGQVIYNSIFRNGLAIRGLPVENLHLISTN 122 Query: 110 IEEMDTVFGGARKLEATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKELGIPV 169 + E D V+GG KLE TI+ A+ER P AIF+ TSC + IIGDD+ESV +++ E GIPV Sbjct: 123 LRERDMVYGGLDKLERTIRDAWERHHPQAIFIATSCPTAIIGDDIESVASQLEAEFGIPV 182 Query: 170 VTCFCEGFKSKIWTSGFDSAYHSIVRKIVK--PPEKKTNKVNIINFWGSDVFSGLLNKLG 227 + CEGFKSK W++GFD+ H I+R+IV+ P K+ + VN+IN WGSDVF +L +LG Sbjct: 183 IPLHCEGFKSKHWSTGFDATQHGILRQIVRKNPERKQEDLVNVINLWGSDVFGPMLGELG 242 Query: 228 YEADYIVPFSTVAQLERISEAAATIQICPTLGTYLGAALEQVYGVPEIKAPVAYGVAGTD 287 +Y+V +TV L ++SEAAAT+ C TL TY+ AALEQ +GVPE+KAP+ YG AGTD Sbjct: 243 LRVNYVVDLATVEDLAQMSEAAATVGFCYTLSTYMAAALEQEFGVPEVKAPMPYGFAGTD 302 Query: 288 AWMRELGRVLDRXXXXXXXXXXXXXXVLPQLEAYRAKLAGTTCYITAGSAHGHALIALLR 347 AW+RE+ RV R V PQLEA R KL G +++ GSA+ H +I +LR Sbjct: 303 AWLREIARVTHREEQAEAYIAREHARVKPQLEALREKLKGIKGFVSTGSAYAHGMIQVLR 362 Query: 348 ELGINVQGAAIFHHDPVYDNGDPAADMLDHTVKTYGDVPNYNVCNKQAYELANILNRVCP 407 ELG+ V G+ +FHHDPVYD+ DP D L H V YGDV +++V N+Q ++ +L RV P Sbjct: 363 ELGVTVDGSLVFHHDPVYDSQDPRQDSLAHLVDNYGDVGHFSVGNRQQFQFYGLLQRVKP 422 Query: 408 DLMIARHGGMTHWGAKLGIPTLLIGDEHFSWGYRGLLNYAERILETLDNREFVTNLAKHS 467 D +I RH G+ ++LGIP + +GDEH + GY+G+LN E IL+ L +R+F ++A H Sbjct: 423 DFIIIRHNGLAPLASRLGIPAIPLGDEHIAVGYQGILNLGESILDVLAHRKFHEDIAAHV 482 Query: 468 TMPYTKWWMEQNPYTF 483 +PY + W+ ++P+ Sbjct: 483 RLPYRQDWLARDPFDL 498 >ref|NP_947693.1| possible nitrogenase molybdenum-iron protein alpha chain (nitrogenase component I) (dinitrogenase) [Rhodopseudomonas palustris CGA009] emb|CAE27789.1| possible nitrogenase molybdenum-iron protein alpha chain (nitrogenase component I) (dinitrogenase) [Rhodopseudomonas palustris CGA009] Length = 511 Score = 425 bits (1092), Expect = e-117, Method: Compositional matrix adjust. Identities = 220/501 (43%), Positives = 305/501 (60%), Gaps = 18/501 (3%) Query: 4 VNLNTPEVPVRELRLGSITGFAGTAAGLLS--------CSNNGGMKDGSR------SFSQ 49 +NL +P RE RLG+IT + G A+ L + C G K G R FSQ Sbjct: 3 INLRSPSAETREQRLGTITSWDGPASRLANESAFSRGDCGPGCGGK-GQRICELESPFSQ 61 Query: 50 CMGCSSGNAFCQLSMITDAAMVNHAPVGCAGDFFGYNFVYRVGQMERNLPPVIGRYFNTN 109 CS A Q S + DA +V H+P+GCA N +R G M R L P ++N Sbjct: 62 GSTCSEQIAESQASNVRDAVLVQHSPIGCAAGHINSNAFFRNGLMRRGLAPRNVTGLSSN 121 Query: 110 IEEMDTVFGGARKLEATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKELGIPV 169 + E D ++GGA KL ATIK A ER +P A+FV TSCA+GIIGDDVESV ELG+PV Sbjct: 122 LIERDMIYGGAEKLRATIKAAVERHQPKAVFVATSCATGIIGDDVESVVRDCEDELGVPV 181 Query: 170 VTCFCEGFKSKIWTSGFDSAYHSIVRKIVKPPE--KKTNKVNIINFWGSDVFSGLLNKLG 227 V +CEGFKSK W+SGFD+ H ++R +VKP ++ N VNII WGSDVF+ +L +LG Sbjct: 182 VAMYCEGFKSKHWSSGFDAIQHGVLRHVVKPKPGPRQDNLVNIIVLWGSDVFTPMLAELG 241 Query: 228 YEADYIVPFSTVAQLERISEAAATIQICPTLGTYLGAALEQVYGVPEIKAPVAYGVAGTD 287 EA+ I+ ++V ++ R SEAAA+ C ++G YLGAALE+ YGV EIKAP YG GTD Sbjct: 242 LEANNILTVASVDEIARASEAAASATFCYSVGGYLGAALEEQYGVQEIKAPQPYGFDGTD 301 Query: 288 AWMRELGRVLDRXXXXXXXXXXXXXXVLPQLEAYRAKLAGTTCYITAGSAHGHALIALLR 347 AW+R L + R V PQ+E R +L G Y+ G+A+ H +I +LR Sbjct: 302 AWLRALAKATGREERAEAYIAREHARVRPQIERLRERLKGVRGYVAMGAAYAHGVIGVLR 361 Query: 348 ELGINVQGAAIFHHDPVYDNGDPAADMLDHTVKTYGDVPNYNVCNKQAYELANILNRVCP 407 ELG+ V G+ +FHHDPVYD+ D D L H + YGDV ++++ N+Q Y+ N+L R P Sbjct: 362 ELGVEVPGSLVFHHDPVYDSHDVRQDTLGHMIDAYGDVEHFSISNRQPYQFYNLLKRSDP 421 Query: 408 DLMIARHGGMTHWGAKLGIPTLLIGDEHFSWGYRGLLNYAERILETLDNREFVTNLAKHS 467 D +I RHGG+ ++LG+P + +GD + GY+G+++ E I++ L R+F +LA H+ Sbjct: 422 DFIIIRHGGLAGLASRLGVPAIALGDASTAIGYQGMIDLGEEIVDALAQRKFHQDLAGHT 481 Query: 468 TMPYTKWWMEQ-NPYTFLGGN 487 ++PY +WW++Q +PY N Sbjct: 482 SLPYKQWWLDQADPYLLARQN 502 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.137 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 747,679,796 Number of extensions: 31080071 Number of successful extensions: 74068 Number of sequences better than 1.0e-05: 266 Number of HSP's gapped: 73598 Number of HSP's successfully gapped: 266 Length of query: 493 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 363 Effective length of database: 404,274,708 Effective search space: 146751719004 Effective search space used: 146751719004 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)