BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0016.p (196 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00014637.1| COG2710: Nitrogenase molybdenum-iron protein,... 151 9e-36 ref|ZP_00014615.1| COG2710: Nitrogenase molybdenum-iron protein,... 144 9e-34 ref|NP_947979.1| possible nitrogenase iron-molybdenum protein su... 141 1e-32 ref|ZP_00060670.1| COG2710: Nitrogenase molybdenum-iron protein,... 139 5e-32 ref|NP_947692.1| possible vanadium nitrogenase associated protei... 136 3e-31 >ref|ZP_00014637.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Rhodospirillum rubrum] Length = 540 Score = 151 bits (381), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 75/181 (41%), Positives = 110/181 (60%), Gaps = 2/181 (1%) Query: 1 VSAWPGLKSAELLENKYGTPFYHFPYLPVGGIETGRFLREVADFSSVDRAAAEAFILDEE 60 +S+W GL A+ LE ++GTP+ H+P LP+G +ETGRFLR VA F+ + A AE I +E Sbjct: 319 LSSWTGLAPAQALEKRFGTPWLHYPVLPIGALETGRFLRAVAAFAGLAPARAETVIAAQE 378 Query: 61 RKFYAHIERTADFMLEFRYGLPRVFYTMLDATYAVGFAKYLLNELGILPAETQYVVDNTP 120 +Y H +R A+F+LE R LP F T+ D+ +G A+ L NE GILP Q+V D P Sbjct: 379 TIYYDHYQRLAEFLLEARCDLPARFVTVTDSFQGLGIARLLTNEFGILPGP-QFVTDTPP 437 Query: 121 EEYRAGIAEQFRKISSRRSAKVEFVEDGGAIQEDIRQKKPKQRA-LILGSGWERDLADEL 179 E R I E + ++ + A V F EDG I++ +R + A ++ GS W+R++ +L Sbjct: 438 EALRPAIIEGYASLADKIGASVHFTEDGEVIRQTLRAAHHRFGAPMVFGSAWDREITQDL 497 Query: 180 G 180 G Sbjct: 498 G 498 >ref|ZP_00014615.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Rhodospirillum rubrum] Length = 470 Score = 144 bits (364), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 77/180 (42%), Positives = 104/180 (57%), Gaps = 2/180 (1%) Query: 1 VSAWPGLKSAELLENKYGTPFYHFPYLPVGGIETGRFLREVADFSSVDRAAAEAFILDEE 60 +S W GL +A L+ KYG P H P +P+G ETG FLREVA F+ +D A EAFI EE Sbjct: 249 LSPWLGLDTARHLDRKYGQPTLHRPIIPIGAKETGAFLREVAAFAGLDSAVVEAFITAEE 308 Query: 61 RKFYAHIERTADFMLEFRYGLPRVFYTMLDATYAVGFAKYLLNELGILPAETQYVVDNTP 120 +Y ++E DF E+ +GLP F + D+ Y + K+L+N+LG++P Q + DN P Sbjct: 309 AVYYRYLEDFTDFYAEYWWGLPAKFAVIGDSAYNLALTKFLVNQLGLIPG-LQIITDNPP 367 Query: 121 EEYRAGIAEQFRKISSRRSAKVEFVEDGGAIQEDIRQKKPKQRALIL-GSGWERDLADEL 179 EE R I + I+ + V F ED I + IR +A IL G+ WERDLA EL Sbjct: 368 EEVREDIRAHYHAIADDVATDVSFEEDSYTIHQKIRATDFGHKAPILFGTTWERDLAKEL 427 >ref|NP_947979.1| possible nitrogenase iron-molybdenum protein subunit nifK homolog [Rhodopseudomonas palustris CGA009] emb|CAE28078.1| possible nitrogenase iron-molybdenum protein subunit nifK homolog [Rhodopseudomonas palustris CGA009] Length = 460 Score = 141 bits (355), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 71/180 (39%), Positives = 107/180 (59%), Gaps = 2/180 (1%) Query: 1 VSAWPGLKSAELLENKYGTPFYHFPYLPVGGIETGRFLREVADFSSVDRAAAEAFILDEE 60 +S W GL +AELL +Y P+ H P +P+G E+G FLR+VA F+ + EAFI EE Sbjct: 239 LSPWLGLSTAELLARRYEQPYLHIPVVPIGARESGAFLRKVAAFAGIPADVVEAFIAKEE 298 Query: 61 RKFYAHIERTADFMLEFRYGLPRVFYTMLDATYAVGFAKYLLNELGILPAETQYVVDNTP 120 +++Y +I+ DF E+ +GLP F + D+ Y + K+L+ +LG++PA+ Q V ++ P Sbjct: 299 KRYYNYIDGFTDFYSEYWWGLPARFAVIGDSAYNLALTKFLVTQLGLIPAK-QIVTEDPP 357 Query: 121 EEYRAGIAEQFRKISSRRSAKVEFVEDGGAIQEDIRQKKPKQR-ALILGSGWERDLADEL 179 E+R I +FR I S VEFVED I + +R + +I G+ WERDL E+ Sbjct: 358 VEHREAIQAEFRAIDEDVSVDVEFVEDSYTIHQALRNADFGHKPPIIFGTTWERDLVKEM 417 >ref|ZP_00060670.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Clostridium thermocellum ATCC 27405] Length = 447 Score = 139 bits (349), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 71/185 (38%), Positives = 109/185 (58%), Gaps = 6/185 (3%) Query: 1 VSAWPGLKSAELLENKYGTPFYHFPYLPVGGIETGRFLREVADFS-----SVDRAAAEAF 55 VS W G A+ L++KYG + F ++P+G +T FL +V +F+ +D+ +A+ F Sbjct: 222 VSPWYGKPIADHLKSKYGQEYTWFHHIPIGANQTEAFLNQVVEFAIEQGADIDKESAQEF 281 Query: 56 ILDEERKFYAHIERTADFMLEFRYGLPRVFYTMLDATYAVGFAKYLLNELGILPAETQYV 115 I E +Y I+ A F+LEFRYGLP + + DA Y V +K+LL+E+GI+P E Q++ Sbjct: 282 IRHESHAYYEEIDNLATFLLEFRYGLPNHAHILHDAGYVVALSKFLLHEVGIVPKE-QFI 340 Query: 116 VDNTPEEYRAGIAEQFRKISSRRSAKVEFVEDGGAIQEDIRQKKPKQRALILGSGWERDL 175 D TPE++ I + S ++ + F D G QE +R K R LI+GSGW+++L Sbjct: 341 TDATPEKFHEAIRADLKSTSDKKEIPLYFEPDAGKAQEILRGIHHKGRGLIIGSGWDKEL 400 Query: 176 ADELG 180 A E G Sbjct: 401 AKEKG 405 >ref|NP_947692.1| possible vanadium nitrogenase associated protein N (U51863) [Rhodopseudomonas palustris CGA009] emb|CAE27788.1| possible vanadium nitrogenase associated protein N (U51863) [Rhodopseudomonas palustris CGA009] Length = 463 Score = 136 bits (343), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 74/183 (40%), Positives = 106/183 (57%), Gaps = 8/183 (4%) Query: 1 VSAWPGLKSAELLENKYGTPFYHFPYLPVGGIETGRFLREVADFSSVDRAAAEAFILDEE 60 +S W GL +A+ LENK+G P+ H P P+GG +T FLR+VA F+ + A A AFI EE Sbjct: 242 LSPWLGLATAQHLENKFGQPYLHLPVPPIGGRQTSEFLRQVAAFAGIPDAQAGAFIAAEE 301 Query: 61 RKFYAHIERTADFMLEFRYGLPRVFYTMLDATYAVGFAKYLLNELGILPAETQYVVDNTP 120 R +Y +IE ++F ++R+GLP F + DA + + + + +LG++PA + DNTP Sbjct: 302 RDYYGYIEAFSEFYSQYRWGLPARFAAIGDAATTLSISSFAVRQLGLVPA-VVVITDNTP 360 Query: 121 EEYRAGIAEQFRKISSRRSAKVEFVEDGGAIQEDIR----QKKPKQRALILGSGWERDLA 176 E+R I F K+ + F ED A+ IR +KP LI G+ WERDLA Sbjct: 361 PEHRDAIRAAFAKLDHDVGIEPVFAEDSYAVHAAIRGADFGRKPP---LIFGTTWERDLA 417 Query: 177 DEL 179 EL Sbjct: 418 KEL 420 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 283,974,913 Number of extensions: 10774600 Number of successful extensions: 24376 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 24366 Number of HSP's successfully gapped: 8 Length of query: 196 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 77 Effective length of database: 434,140,797 Effective search space: 33428841369 Effective search space used: 33428841369 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 122 (51.6 bits)