BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0006.p (340 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_241232.1| BH0366~unknown conserved protein in others [Bac... 200 4e-50 ref|NP_831696.1| Low-affinity zinc transport protein [Bacillus c... 169 1e-40 ref|NP_978325.1| cobalamin synthesis protein/P47K family protein... 166 5e-40 ref|NP_655789.1| cobW, Cobalamin synthesis protein/P47K [Bacillu... 165 1e-39 ref|NP_763743.1| cobalamin synthesis related protein CobW [Staph... 161 2e-38 >ref|NP_241232.1| BH0366~unknown conserved protein in others [Bacillus halodurans] pir||F83695 hypothetical protein BH0366 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04085.1| BH0366~unknown conserved protein in others [Bacillus halodurans] Length = 311 Score = 200 bits (508), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 121/322 (37%), Positives = 186/322 (57%), Gaps = 19/322 (5%) Query: 7 VYILSGFLGSGKTTLLQRLLDHWKAQGLRPAVVMNELGEVNLDGLLVEQSVPMAEMLGGC 66 +YILSGFLGSGK+TLL+RL++ +A+G + V+MNELGEV++D +V + P+ EML GC Sbjct: 8 LYILSGFLGSGKSTLLKRLIEQEQARGNKIGVLMNELGEVSVDSSIVPKDTPLKEMLNGC 67 Query: 67 ICCSIRGDLSTELATLIKKESPDVVVIEATGAANPLEIVDAVTEISLYQLVELKGLITVV 126 ICC+I+G+LS +L + I++ DV+ IEATGAA+PLE+V+A T V ++G++TVV Sbjct: 68 ICCTIQGELSAQLQSFIEEYELDVIFIEATGAAHPLEVVEACTHPVFAGQVRVRGVLTVV 127 Query: 127 DAAHLIELYRSQQGATYRLMQEQIRCASVLILNKTDRVTPQEADEITVILRKWNAYAEIL 186 +A IE S + +LM+ Q+ A ++++NK D+VT + +++T ++ N A + Sbjct: 128 NARQWIEGKMSIK--IKKLMEAQVTFADLIVVNKVDQVTDAQVEQLTTSIKDLNPKAINI 185 Query: 187 PAVRCALEPEALLEGMGGIHAEARFQEDDEMQAGHAGDSSEAGAAVHGSHDHVMAYTHYF 246 P LEP +H+E R A + SE+ A VH H H+ Sbjct: 186 PTTFSNLEP---------VHSE-RLHH----AAAPSSRGSESKAHVH-EHLHLRTMAVPI 230 Query: 247 KRPVNSEEFEQFVKELPRDVYRAKGIVTFSDTSSRFLFQYAYREADFMKI-TPQGDVPDV 305 + PV+ E F+K + +YR KG + ++ FLF YAY + F + T Q P V Sbjct: 231 QHPVDRMELLSFLKGIQGQIYRVKGFIHLKESPGLFLFNYAYGDPMFERHRTDQAYAP-V 289 Query: 306 AVFIGEHFSSSELRTRLLQLEK 327 VFIG+ + L QL++ Sbjct: 290 LVFIGDDLDHQAIEEGLQQLQQ 311 >ref|NP_831696.1| Low-affinity zinc transport protein [Bacillus cereus ATCC 14579] gb|AAP08897.1| Low-affinity zinc transport protein [Bacillus cereus ATCC 14579] Length = 304 Score = 169 bits (428), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 103/328 (31%), Positives = 173/328 (52%), Gaps = 34/328 (10%) Query: 5 MPVYILSGFLGSGKTTLLQRLLDHWKAQGLRPAVVMNELGEVNLDGLLVEQSVPMAEMLG 64 M ++IL GFLGSGK+TLLQ LL K + + AV+MNE+GE ++D ++ + + E+L Sbjct: 1 MEIHILGGFLGSGKSTLLQNLLLAEKKKNRKVAVLMNEIGEYSVDTDIIGKENVLRELLK 60 Query: 65 GCICCSIRGDLSTELATLIKKESPDVVVIEATGAANPLEIVDAVTEISLYQLVELKGLIT 124 GCICC+++ +L +L +L ++E PDV+ IE TG A+P+E++DA L +E+K ++ Sbjct: 61 GCICCTLKEELEIQLHSLYQQERPDVIYIETTGVAHPIEVLDACVSPILAPFLEVKSIVV 120 Query: 125 VVDAAHLIELYRSQQGATY-RLMQEQIRCASVLILNKTDRVTPQEADEITVILRKWNAYA 183 V+DA L RS A +L+ EQ++ S +++NK D +T ++ + + ++ N +A Sbjct: 121 VLDAVRW--LNRSILSANVQQLLHEQLKFGSHILVNKADLLTDEDKNTVFEEVKAINDHA 178 Query: 184 EILPAVRCALEPEALLEGMGGIHAEARFQEDDEMQAGHAGDSSEAGAAVHGSHDHVMAYT 243 ++ C + + + EA F D+E + H H H+ T Sbjct: 179 KMYETKYCNISLKDI--------EEAEFPNDEEHETLHV-----------KQHLHIQTMT 219 Query: 244 HYFKRPVNSEEFEQFVKELPRDVYRAKGIVTFSDTSSRFLFQYAY-----READFMKITP 298 + F +P++ ++ +++ LP +YR KG V F LFQY++ E DF Sbjct: 220 YQFTKPIDQDKLYEWLSNLPDSIYRVKGFVKFHGDKYPHLFQYSFGVPTLLEQDF----- 274 Query: 299 QGDVPDVAVFIGEHFSSSELRTRLLQLE 326 P V IGE +L L ++E Sbjct: 275 --GFPTNLVVIGEGLDKKQLAEGLEKVE 300 >ref|NP_978325.1| cobalamin synthesis protein/P47K family protein [Bacillus cereus ATCC 10987] gb|AAS40933.1| cobalamin synthesis protein/P47K family protein [Bacillus cereus ATCC 10987] Length = 318 Score = 166 bits (421), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 102/328 (31%), Positives = 171/328 (52%), Gaps = 34/328 (10%) Query: 7 VYILSGFLGSGKTTLLQRLLDHWKAQGLRPAVVMNELGEVNLDGLLVEQSVPMAEMLGGC 66 ++IL GFLGSGK+TLLQ LL K + + AV+MNE+GE ++D ++ + + E+L GC Sbjct: 19 IHILGGFLGSGKSTLLQNLLLAEKKKNRKVAVLMNEIGEYSVDTDIIGKENVLRELLKGC 78 Query: 67 ICCSIRGDLSTELATLIKKESPDVVVIEATGAANPLEIVDAVTEISLYQLVELKGLITVV 126 ICC+++ +L +L +L ++E PDV+ IE TG A+P+E++DA L +E+K ++ V+ Sbjct: 79 ICCTLKEELEIQLHSLYQQERPDVIYIETTGVAHPIEVLDACVSPILAPFLEVKSIVVVL 138 Query: 127 DAAHLIELYRSQQGATY-RLMQEQIRCASVLILNKTDRVTPQEADEITVILRKWNAYAEI 185 DA L RS A +L+ EQ++ S +++NK+D +T + +++ ++ N +A++ Sbjct: 139 DAVRW--LNRSVLSANVQQLLHEQLKFGSHILINKSDLLTGADKNKVIEEVKVINNHAKL 196 Query: 186 LPAVRCALEPEALLEGMGGIHAEARFQEDDEMQAGHAGDSSEAGAAVHGSHDHVMAYTHY 245 C + E + EA F D E + H H H+ T+ Sbjct: 197 FETKYCNISLEDI--------EEAEFTNDAEHETLHV-----------KQHLHIQTMTYQ 237 Query: 246 FKRPVNSEEFEQFVKELPRDVYRAKGIVTFSDTSSRFLFQYAY-----READFMKITPQG 300 F + ++ ++ +++ LP +YR KG V F LFQY++ E DF Sbjct: 238 FTKSIDQDKLYEWLSNLPDSIYRVKGFVKFHGDKYPHLFQYSFGVPTLLEQDF------- 290 Query: 301 DVPDVAVFIGEHFSSSELRTRLLQLEKR 328 P V IGE +L L ++E Sbjct: 291 GFPTNLVVIGEGLDKKQLAEGLEEVENN 318 >ref|NP_655789.1| cobW, Cobalamin synthesis protein/P47K [Bacillus anthracis A2012] ref|NP_844334.1| cobalamin synthesis protein/P47K family protein [Bacillus anthracis str. Ames] gb|AAP25820.1| cobalamin synthesis protein/P47K family protein [Bacillus anthracis str. Ames] Length = 307 Score = 165 bits (418), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 101/326 (30%), Positives = 170/326 (52%), Gaps = 34/326 (10%) Query: 7 VYILSGFLGSGKTTLLQRLLDHWKAQGLRPAVVMNELGEVNLDGLLVEQSVPMAEMLGGC 66 ++IL GFLGSGK+TLLQ LL K + + AV+MNE+GE ++D ++ + + E+L GC Sbjct: 6 IHILGGFLGSGKSTLLQNLLLAEKKKNRKVAVLMNEIGEYSVDTDIIGKENVLRELLKGC 65 Query: 67 ICCSIRGDLSTELATLIKKESPDVVVIEATGAANPLEIVDAVTEISLYQLVELKGLITVV 126 ICC+++ +L +L +L ++E PDV+ IE TG A+P+E++DA L +E+K ++ V+ Sbjct: 66 ICCTLKEELEIQLHSLYQQERPDVIYIETTGVAHPIEVLDACVSPILAPFLEVKSIVVVL 125 Query: 127 DAAHLIELYRSQQGATY-RLMQEQIRCASVLILNKTDRVTPQEADEITVILRKWNAYAEI 185 DA L RS A +L+ EQ++ S +++NK+D + + +++ ++ N +A++ Sbjct: 126 DAVRW--LNRSVLSANVQQLLHEQLKFGSHILINKSDLLIEADKNKVIEEVKAINNHAKL 183 Query: 186 LPAVRCALEPEALLEGMGGIHAEARFQEDDEMQAGHAGDSSEAGAAVHGSHDHVMAYTHY 245 C + E + EA F D E + H H H+ T+ Sbjct: 184 FETKYCNISLEDI--------EEAEFTNDTEHETLHV-----------KQHLHIQTMTYQ 224 Query: 246 FKRPVNSEEFEQFVKELPRDVYRAKGIVTFSDTSSRFLFQYAY-----READFMKITPQG 300 F + ++ ++ +++ LP +YR KG V F LFQY++ E DF Sbjct: 225 FTKSIDQDKLYEWLSNLPDSIYRVKGFVKFHGDKYPHLFQYSFGVPTLLEQDF------- 277 Query: 301 DVPDVAVFIGEHFSSSELRTRLLQLE 326 P V IGE +L L ++E Sbjct: 278 GFPTNLVVIGEGLDKKQLAEGLEKVE 303 >ref|NP_763743.1| cobalamin synthesis related protein CobW [Staphylococcus epidermidis ATCC 12228] gb|AAO03785.1| cobalamin synthesis related protein CobW [Staphylococcus epidermidis ATCC 12228] Length = 293 Score = 161 bits (407), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 102/317 (32%), Positives = 158/317 (49%), Gaps = 29/317 (9%) Query: 5 MPVYILSGFLGSGKTTLLQRLLDHWKAQGLRPAVVMNELGEVNLDGLLVEQ-SVPMAEML 63 M + I+ GFLGSGKT+ + L+ L PAV+MNE G+ ++DG L+E+ VPM+E+ Sbjct: 1 MEIIIIGGFLGSGKTSAINHLIADALENNLNPAVIMNEFGKRSVDGQLIERPEVPMSEIT 60 Query: 64 GGCICCSIRGDLSTELATLIKKESPDVVVIEATGAANPLEIVDAVTEISLYQLVELKGLI 123 GCICC+++ D+S +L L K PD++ IE +G A PL +VDA L + L+ ++ Sbjct: 61 EGCICCAMKSDVSQQLHELYLKYQPDIIFIECSGVAEPLAVVDACFTPVLAPFITLRSMV 120 Query: 124 TVVDAAHLIELYRSQQGATYRLMQEQIRCASVLILNKTDRVTPQEADEITVILRKWNAYA 183 ++DA+ + +S L EQ+R + L +NK D++ +E + L + N+ A Sbjct: 121 GIIDAS-MYSRIKSYPQDIAALFYEQLRHCATLFVNKIDKIEVEETARLLRQLERLNSDA 179 Query: 184 EILPAVRCALEPEALLEGMGGIHAEARFQEDDEMQAGHAGDSSEAGAAVHGSHDHVMAYT 243 I L ++LLE H +S A +H + +H Sbjct: 180 NIQVGQFGELNLKSLLE------------------PTHI--NSNACGTLHSNINH----- 214 Query: 244 HYFKRP--VNSEEFEQFVKELPRDVYRAKGIVTFSDTSSRFLFQYAYREADFMKITPQGD 301 + + P EE + LP+DVYR KG V FSD +L QYA + I + D Sbjct: 215 QFIENPRLQTKEEMISALDNLPQDVYRVKGFVRFSDQQHVYLVQYAQGNIELSPIQLKND 274 Query: 302 VPDVAVFIGEHFSSSEL 318 VP + IG+H +L Sbjct: 275 VPLYLIVIGKHLKQIQL 291 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 465,334,759 Number of extensions: 18296089 Number of successful extensions: 63514 Number of sequences better than 1.0e-05: 575 Number of HSP's gapped: 61808 Number of HSP's successfully gapped: 577 Length of query: 340 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 214 Effective length of database: 415,135,104 Effective search space: 88838912256 Effective search space used: 88838912256 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)