BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0032.p (452 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00180650.1| hypothetical protein Mther134901 [Moorella th... 398 e-109 ref|ZP_00060410.1| COG0773: UDP-N-acetylmuramate-alanine ligase ... 395 e-108 ref|NP_954110.1| UDP-N-acetylmuramate--alanine ligase [Geobacter... 386 e-106 ref|ZP_00080495.1| COG0773: UDP-N-acetylmuramate-alanine ligase ... 382 e-104 ref|NP_624095.1| UDP-N-acetylmuramate-alanine ligase [Thermoanae... 370 e-101 >ref|ZP_00180650.1| hypothetical protein Mther134901 [Moorella thermoacetica ATCC 39073] Length = 468 Score = 398 bits (1023), Expect = e-109, Method: Compositional matrix adjust. Identities = 205/451 (45%), Positives = 299/451 (66%), Gaps = 5/451 (1%) Query: 2 HFIGIGGYGMSAIARVMLEMGYTVTGSDVAAQELTEKLIAKGAKVYIGHTAEQV-KGADL 60 HF+GIGG GMSA+AR++L GY V+GSD + T L A GA +Y H A + G Sbjct: 11 HFVGIGGVGMSALARILLAQGYRVSGSDPKENQFTRSLEAAGAIIYHQHDAANLAPGVQE 70 Query: 61 VVYSTALASDNVEWVEAERLNIPVLHRSQMLARLLNERKGVAIAGAHGKTTTSSMIALIM 120 VV S+A+ S N E V A + ++PV+ R ++LARL N R+G+A+AGAHGKTTTS+++AL+M Sbjct: 71 VVISSAVPSSNPEVVAARQRSLPVVKRGELLARLFNARRGIAVAGAHGKTTTSALVALVM 130 Query: 121 EECGVDPTYIIGGEIMNVGTNAKAGQGEYVVAEADESDGSFLQYHPWLGIVTNIEADHLE 180 +E G++P +IGG + +NA G+G+++VAEADESDGSFL P + ++TNIEADHLE Sbjct: 131 KEGGLEPAAVIGGYVREFASNAYPGRGDFLVAEADESDGSFLWLKPEIALITNIEADHLE 190 Query: 181 NYGGDFGRLKAAYVQFMNQMQEDGTAIVCADDETAASLLPEVKGNVITYGIHSPSADYTA 240 +Y G R+ AA+ F++Q++ G AI+CA+D A L+ VITYG++ DY A Sbjct: 191 HY-GSLDRIVAAFKDFIDQIRPGGKAILCAEDPRVAGLVACSPRQVITYGLNG-RPDYRA 248 Query: 241 TDIVLGDRQVAYTMNFGSAVLGRIELSIPGKYNLYNSMAAVIACLKAGIPFQGIAESILK 300 T + + + + LG++ +++PG++N+ N++ A+ A + GIPF +A ++ + Sbjct: 249 TGVQMAGMGGRAAIYYREQYLGQLTMAVPGRHNILNALGAIAAGHQLGIPFAVMARALGQ 308 Query: 301 FHGAKRRFQVLGEANDILVIDDYAHHPTEIQATISAAKATG-KRIIAVFQPQRYTRTFFL 359 F G RRF++L + V+DDYAHHPTEI+AT++AA G KR++AVFQP RYTRT L Sbjct: 309 FRGVGRRFEILWDDGTTRVVDDYAHHPTEIRATLAAASQVGAKRVVAVFQPHRYTRTHHL 368 Query: 360 LDAFSRAFSEADEVILTDIYSPAGEKQIEGVSSAKLVELIVQNSNANARHLPTKDAVLAD 419 F +AF +AD VI+ DIY PAGE + GV+S + I + + +LPT + LA Sbjct: 369 YREFGQAFRQADVVIVNDIY-PAGEAPLPGVNSQLITGEIKGSGHQQVYYLPTLEETLAF 427 Query: 420 LQDRIAPGDLVITMGAGDIWKVGYALAEGLK 450 L+ PGDLV+T+GAGD+W+VG LA+ L+ Sbjct: 428 LKKSCRPGDLVLTLGAGDVWRVGMGLAQYLE 458 >ref|ZP_00060410.1| COG0773: UDP-N-acetylmuramate-alanine ligase [Clostridium thermocellum ATCC 27405] Length = 472 Score = 395 bits (1014), Expect = e-108, Method: Compositional matrix adjust. Identities = 202/445 (45%), Positives = 297/445 (66%), Gaps = 6/445 (1%) Query: 1 VHFIGIGGYGMSAIARVMLEMGYTVTGSDVAAQELTEKLIAKGAKVYIGHTAEQVKGADL 60 VHFIGIGG MS +A ++L +G+TV+GSD+ + +T KL KG K+Y GH+ E +K ADL Sbjct: 18 VHFIGIGGISMSGLAEILLNLGFTVSGSDIRSSNITSKLEKKGIKIYTGHSEENIKDADL 77 Query: 61 VVYSTALASDNVEWVEAERLNIPVLHRSQMLARLLNERK-GVAIAGAHGKTTTSSMIALI 119 VVY+ A+ SDN E +A+ L IP L R+ +L ++ + VA++G HGKTTT+SMI+LI Sbjct: 78 VVYTAAVKSDNPELAKAKSLGIPTLDRATLLGEIMKKYPFSVAVSGTHGKTTTTSMISLI 137 Query: 120 MEECGVDPTYIIGGEIMNVGTNAKAGQGEYVVAEADESDGSFLQYHPWLGIVTNIEADHL 179 M + +DPT IGGE+ +G N + G +Y VAEA E SFL++HP+L +V NIEADHL Sbjct: 138 MLKSALDPTIHIGGELDAIGGNTRIGSNKYFVAEACEYVESFLKFHPYLAVVLNIEADHL 197 Query: 180 ENYGGDFGRLKAAYVQFMNQMQEDGTAIVCADDETAASLLPEVKGNVITYGIHSPSADYT 239 + Y D +K A+++F+ + E+G + CADD+ S+L +V N+IT+G++S +A +T Sbjct: 198 D-YFKDIEHIKNAFLKFIRLVPENGYVVACADDQNVLSILKDVHCNIITFGLNSKNAMWT 256 Query: 240 ATDIVLGDRQV-AYTMNFGSAVLGRIELSIPGKYNLYNSMAAVIACLKAGIPFQGIAESI 298 A DI + A+T+ + + I+L +PG +N+ N++A++ AC G + I + + Sbjct: 257 AEDISFNENGFAAFTVLKDNERITNIQLKVPGIHNISNALASIAACYALGCSIENIKKGL 316 Query: 299 LKFHGAKRRFQVLGEANDILVIDDYAHHPTEIQATISAAKATG-KRIIAVFQPQRYTRTF 357 + G RRF++ G N+I V+DDYAHHP+EI+AT+ AA++ RI VFQP YTRT Sbjct: 317 ESYTGIHRRFELKGIENNIKVVDDYAHHPSEIKATLKAARSGNYPRIWTVFQPHTYTRTK 376 Query: 358 FLLDAFSRAFSEADEVILTDIYSPAGEKQIEGVSSAKLVELIVQNSNANARHLPTKDAVL 417 FLLD FS+AF + D+VI+TDIYS A EK + S L E I +N +A +LP + ++ Sbjct: 377 FLLDEFSKAFKDTDKVIITDIYS-AREKDTGEIHSRILAEKIRENGQ-DAIYLPDFEGIV 434 Query: 418 ADLQDRIAPGDLVITMGAGDIWKVG 442 L+ +PGDL+ITMGAGD++KVG Sbjct: 435 EYLKKNASPGDLIITMGAGDVYKVG 459 >ref|NP_954110.1| UDP-N-acetylmuramate--alanine ligase [Geobacter sulfurreducens PCA] gb|AAR36460.1| UDP-N-acetylmuramate--alanine ligase [Geobacter sulfurreducens PCA] Length = 462 Score = 386 bits (991), Expect = e-106, Method: Compositional matrix adjust. Identities = 201/453 (44%), Positives = 297/453 (65%), Gaps = 4/453 (0%) Query: 1 VHFIGIGGYGMSAIARVMLEMGYTVTGSDVAAQELTEKLIAKGAKVYIGHTAEQVKGADL 60 +HF+GIGG GMS IA V+L +GY V+GSD+ + T++L + G ++ GH E V D+ Sbjct: 8 IHFVGIGGIGMSGIAEVLLNLGYKVSGSDLKQSDTTDRLASLGGEIRFGHGRENVADVDV 67 Query: 61 VVYSTALASDNVEWVEAERLNIPVLHRSQMLARLLNERKGVAIAGAHGKTTTSSMIALIM 120 VV STA+ DN E VEA+R IPV+ R++MLA L+ + G+AIAG HGKTTT+SM+A ++ Sbjct: 68 VVTSTAVRDDNPEVVEAKRRMIPVIPRAEMLAELMRMKYGIAIAGTHGKTTTTSMVATVL 127 Query: 121 EECGVDPTYIIGGEIMNVGTNAKAGQGEYVVAEADESDGSFLQYHPWLGIVTNIEADHLE 180 G+DPT +IGG++ +GTNAK GQG+++VAEADESDGSFL+ P + +VTNI+ADHL+ Sbjct: 128 THGGIDPTIVIGGKLNTLGTNAKLGQGQFLVAEADESDGSFLKLSPTIAVVTNIDADHLD 187 Query: 181 NYGGDFGRLKAAYVQFMNQMQEDGTAIVCADDETAASLLPEVKGNVITYGIHSPSADYTA 240 Y G ++K +V F+N++ G A++C +D A +LP VK +TYG+ S AD A Sbjct: 188 FYTGGIEQIKDTFVDFINKIPFYGLAVLCLEDRNIAEILPRVKKRFVTYGL-SSQADIRA 246 Query: 241 TDIVLGDRQVAYTMNFGSAVLGRIELSIPGKYNLYNSMAAVIACLKAGIPFQGIAESILK 300 T I L ++ ++ +G + S+PG +N+ N++A + ++ +PF I E Sbjct: 247 THIRLEGGTTSFVAHYKGYRMGEVSFSMPGAHNVLNALACIAVAMELDVPFSQIQEGFAT 306 Query: 301 FHGAKRRFQVLGEANDILVIDDYAHHPTEIQATISAAKA--TGKRIIAVFQPQRYTRTFF 358 F G RRFQV GE N I V+DDY HHP EI+AT++A K+ +R++ FQP R++RT Sbjct: 307 FGGVGRRFQVKGEVNGITVVDDYGHHPAEIRATLAAGKSGWPERRLVVAFQPHRFSRTRE 366 Query: 359 LLDAFSRAFSEADEVILTDIYSPAGEKQIEGVSSAKLVELIVQNSNANARHLPTKDAVLA 418 L F F +AD ++LTDIY PAGE IEGVS+ +L E I ++ + ++ ++A+ Sbjct: 367 LFAEFVTCFYDADVLVLTDIY-PAGEAPIEGVSAERLAEEIRKHGQRDVTYVADREALPE 425 Query: 419 DLQDRIAPGDLVITMGAGDIWKVGYALAEGLKK 451 L + PGD+V+T+GAG+IW+ G A L++ Sbjct: 426 HLLTIVQPGDIVLTLGAGNIWQSGEAFLAKLEE 458 >ref|ZP_00080495.1| COG0773: UDP-N-acetylmuramate-alanine ligase [Geobacter metallireducens] Length = 461 Score = 382 bits (980), Expect = e-104, Method: Compositional matrix adjust. Identities = 199/452 (44%), Positives = 296/452 (65%), Gaps = 4/452 (0%) Query: 1 VHFIGIGGYGMSAIARVMLEMGYTVTGSDVAAQELTEKLIAKGAKVYIGHTAEQVKGADL 60 +HF+GIGG GMS IA V+L +GY V+GSD+ + T++L + G + GH E + D+ Sbjct: 8 IHFVGIGGIGMSGIAEVLLNLGYKVSGSDLKQSDTTDRLASLGGDIRFGHARENLADVDV 67 Query: 61 VVYSTALASDNVEWVEAERLNIPVLHRSQMLARLLNERKGVAIAGAHGKTTTSSMIALIM 120 VV STA+ DN E +EA+R IPV+ R++MLA L+ + G+AIAG HGKTTT+SM+A + Sbjct: 68 VVTSTAVHDDNPEVMEAKRRMIPVIPRAEMLAELMRMKYGIAIAGTHGKTTTTSMVATAL 127 Query: 121 EECGVDPTYIIGGEIMNVGTNAKAGQGEYVVAEADESDGSFLQYHPWLGIVTNIEADHLE 180 G+DPT +IGG++ +GTNAK GQG+++VAEADESDGSFL+ P + +VTNI+ADHL+ Sbjct: 128 THGGIDPTIVIGGKLNTLGTNAKLGQGQFLVAEADESDGSFLKLSPTIAVVTNIDADHLD 187 Query: 181 NYGGDFGRLKAAYVQFMNQMQEDGTAIVCADDETAASLLPEVKGNVITYGIHSPSADYTA 240 Y G ++K +V F+N++ G A++C +D A +LP VK +TYG+ S AD A Sbjct: 188 FYTGGIEQIKDTFVDFINKIPFYGLAVLCLEDRNIAEILPRVKKRFVTYGL-SSQADIRA 246 Query: 241 TDIVLGDRQVAYTMNFGSAVLGRIELSIPGKYNLYNSMAAVIACLKAGIPFQGIAESILK 300 T I L ++ ++ +G + ++PG +N+ N++A + ++ +PF I E K Sbjct: 247 THIRLEGGTTSFVAHYKGYRMGEVSFAMPGAHNVLNALACIAVAMELDVPFAQIQEGFAK 306 Query: 301 FHGAKRRFQVLGEANDILVIDDYAHHPTEIQATISAAKA--TGKRIIAVFQPQRYTRTFF 358 F G RRFQV GE N I V+DDY HHP EI+AT++A K+ +R++ FQP R++RT Sbjct: 307 FGGVGRRFQVKGEVNGITVVDDYGHHPAEIRATLAAGKSGWPDRRLVVAFQPHRFSRTKE 366 Query: 359 LLDAFSRAFSEADEVILTDIYSPAGEKQIEGVSSAKLVELIVQNSNANARHLPTKDAVLA 418 L F F +AD ++LTDIYS AGE IEGV++ +L E I ++ + ++ ++A+ Sbjct: 367 LFAEFVTCFYDADVLVLTDIYS-AGEAPIEGVTAERLAENIRKHGQRDVTYIADREALPD 425 Query: 419 DLQDRIAPGDLVITMGAGDIWKVGYALAEGLK 450 L + PGD+V+T+GAG+IW+ G AL L+ Sbjct: 426 HLLQIVRPGDIVLTLGAGNIWQTGEALLRKLE 457 >ref|NP_624095.1| UDP-N-acetylmuramate-alanine ligase [Thermoanaerobacter tengcongensis] sp|Q8R749|MURC_THETN UDP-N-acetylmuramate--L-alanine ligase (UDP-N-acetylmuramoyl-L-alanine synthetase) gb|AAM25699.1| UDP-N-acetylmuramate-alanine ligase [Thermoanaerobacter tengcongensis] Length = 460 Score = 370 bits (949), Expect = e-101, Method: Compositional matrix adjust. Identities = 191/454 (42%), Positives = 295/454 (64%), Gaps = 7/454 (1%) Query: 1 VHFIGIGGYGMSAIARVMLEMGYTVTGSDVAAQELTEKLIAKGAKVYIGHTAEQVKGADL 60 +HFIGIGG MS +A ++LE G+T+TGSD+ L E+L +GAK+ I H AE V GADL Sbjct: 11 IHFIGIGGISMSGLAHILLEEGHTITGSDIKNSHLIERLEKEGAKINIPHMAESVIGADL 70 Query: 61 VVYSTALASDNVEWVEAERLNIPVLHRSQMLARLLNERK-GVAIAGAHGKTTTSSMIALI 119 VVY+ A+ DNVE+ +A+ L IP++ R+ +L ++ + K GVA+AG+HGKTTT+S+I++I Sbjct: 71 VVYTAAIHDDNVEYQKAKELGIPIIDRATLLGLIMKKYKFGVAVAGSHGKTTTTSLISVI 130 Query: 120 MEECGVDPTYIIGGEIMNVGTNAKAGQGEYVVAEADESDGSFLQYHPWLGIVTNIEADHL 179 ++ G DPT ++GGEI +G N + G+ EY V EA E SFL+++P++ ++ N+++DHL Sbjct: 131 LDRAGYDPTVLVGGEIDAIGGNVRVGKSEYFVTEACEYTDSFLKFYPYIAVILNVDSDHL 190 Query: 180 ENYGGDFGRLKAAYVQFMNQMQEDGTAIVCADDETAASLLPEVKGNVITYGIHSPSADYT 239 +Y + +K ++ QF + + DG + C DD ++ ++ N++TYGI+ S D+ Sbjct: 191 -DYFKNIDNIKQSFRQFASLVPSDGFVVACKDDANTMHVIKGLEKNIVTYGINEKS-DWQ 248 Query: 240 ATDIVLGDRQVA-YTMNFGSAVLGRIELSIPGKYNLYNSMAAVIACLKAGIPFQGIAESI 298 A DI ++ A + + + +G +LSIPGK+N+YN++A++ G+ + E I Sbjct: 249 AKDITYDEKGCASFNVYYRGKFIGHFKLSIPGKHNIYNALASLAVTYLLGVDTEKAKEYI 308 Query: 299 LKFHGAKRRFQVLGEANDILVIDDYAHHPTEIQATISAAKA-TGKRIIAVFQPQRYTRTF 357 +F G RRF+V G + V+DDYAHHP EI AT+ AAK KRIIA+FQP Y+RT Sbjct: 309 KEFKGTHRRFEVKGVVEGVTVVDDYAHHPAEIHATLEAAKNYPHKRIIAIFQPHTYSRTK 368 Query: 358 FLLDAFSRAFSEADEVILTDIYSPAGEKQIEGVSSAKLVELIVQNSNANARHLPTKDAVL 417 LL F+ +F AD++I+TDIY+ A EK VSS LV+LI + + ++ D+++ Sbjct: 369 ALLSDFAESFDAADKIIITDIYA-AREKDTGIVSSKDLVDLIFKRGK-DVLYIKDFDSIV 426 Query: 418 ADLQDRIAPGDLVITMGAGDIWKVGYALAEGLKK 451 L++ GDLV+T+GAG+I++VG E KK Sbjct: 427 EYLKENTEEGDLVLTIGAGNIYEVGEKFLEENKK 460 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 635,145,759 Number of extensions: 25270416 Number of successful extensions: 73015 Number of sequences better than 1.0e-05: 1248 Number of HSP's gapped: 71060 Number of HSP's successfully gapped: 1261 Length of query: 452 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 323 Effective length of database: 406,989,807 Effective search space: 131457707661 Effective search space used: 131457707661 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)