BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0019.p (280 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_243881.1| protease (processing of pro-sigma-K to active s... 137 2e-31 ref|NP_834152.1| Stage IV sporulation protein FB [Bacillus cereu... 133 5e-30 ref|NP_658478.1| S2Pmetalloprt, S2P metal dependent membrane ass... 132 7e-30 ref|NP_980831.1| stage IV sporulation protein FB [Bacillus cereu... 131 1e-29 ref|ZP_00097333.1| COG1994: Zn-dependent proteases [Desulfitobac... 105 1e-21 >ref|NP_243881.1| protease (processing of pro-sigma-K to active sigma-K) [Bacillus halodurans] pir||G84026 proteinase (processing of pro-sigma-K to active sigma-K) spoIVFB [imported] - Bacillus halodurans (strain C-125) dbj|BAB06734.1| protease (processing of pro-sigma-K to active sigma-K) [Bacillus halodurans] Length = 289 Score = 137 bits (346), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 82/271 (30%), Positives = 138/271 (50%), Gaps = 6/271 (2%) Query: 8 QLALHPLFVLIMLLSVVTGQFLELLTLFTIVFIHEMGHVCAALLCGVQVKSVQLLPFGGV 67 ++ ++PLF ++ + V+TG F E+L +F IVF+HEMGH AA +++ ++LLPFGGV Sbjct: 10 KMKVNPLFWFVIGMGVLTGYFREVLMVFVIVFVHEMGHAVAAHFFRWRIRKIELLPFGGV 69 Query: 68 AVMEDHGRLTAVREMGIAIAGPLQNVLLIVMAYGLRQTGYGTSAFLDYFIQANAIIALFN 127 A +E+ G + E+ I AGPLQ+V ++ +YGL + F+ N I FN Sbjct: 70 AEVEEGGNRSFKEEVIIITAGPLQHVWMMAASYGLVALEMWNVSTYQQFLWYNVTILAFN 129 Query: 128 LLPVLPLDGGKIFQAMXXXXXXXXXXXXWCGRVSIAASVLVVGYAMXXXXXXXXXXXXXX 187 LLP+LPLDGG++ Q + R ++ S L + A+ Sbjct: 130 LLPILPLDGGRLMQLLCMRFWPYRLAL----RRTVGLSFLFLTVAVCVAAILYPFHLNLW 185 Query: 188 MIGAFLLYSNLTDQRNLPYRFVSFLMNREAVYERYQQSGSAARPIVAFSAKPLDDILRLF 247 ++ FL+ +N + + YRF+ FL R Y Q G P+V + + + ++ + F Sbjct: 186 LVLTFLIVNNYLEYKQCHYRFIRFLTER--FYIGLSQQGKPHLPVVVSAHQEVSEVAKQF 243 Query: 248 KRNQYHFIYVMNEDKSIVAVVPEQKLISSYF 278 +R H I V + + +PE +++ + F Sbjct: 244 RRGVIHTIIVNDAKQKRTHYLPETEVLRALF 274 >ref|NP_834152.1| Stage IV sporulation protein FB [Bacillus cereus ATCC 14579] gb|AAP11353.1| Stage IV sporulation protein FB [Bacillus cereus ATCC 14579] Length = 286 Score = 133 bits (334), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 87/287 (30%), Positives = 145/287 (50%), Gaps = 18/287 (6%) Query: 1 LIRIRGI--QLALHPLFVLIMLLSVVTGQFLELLTLFTIVFIHEMGHVCAALLCGVQVKS 58 +I+ R + ++++HPLF +I+++ + T +F ELL LF IV IHE+GH AA ++K Sbjct: 1 MIKYRDVLTKISVHPLFWVIIVIGIFTARFKELLLLFCIVLIHELGHAFAAAHYNWRIKR 60 Query: 59 VQLLPFGGVAVMEDHGRLTAVREMGIAIAGPLQNVLLIVMAYGLRQTGYGTSAFLDYFIQ 118 +QLLPFGGVA +E+HG + E+ + IAGP+Q+V ++++ Y L + G+ + +F+ Sbjct: 61 IQLLPFGGVAELEEHGNKSLKEELVVVIAGPIQHVWMMLVGYMLFKAGWLNADLYYFFMW 120 Query: 119 ANAIIALFNLLPVLPLDGGKIFQAMXXXXXXXXXXXXWCGRVSIAASVLVVGYAMXXXXX 178 N II FNLLP+ PLDGGK+ + ++S +++G+ + Sbjct: 121 NNIIILAFNLLPIWPLDGGKVLFNVLSYRFPYLQAHEKMMQLSCVFFSVILGWQLLWNSN 180 Query: 179 XXXXXXXXXMIGAFLLYSNLTDQRNLPYRFVSFLMNREAVYERYQQSGSAARPIVAFSAK 238 + A LY +R Y F+ FL+ R Y ++ PI + Sbjct: 181 NIMMWVLLIFL-AVSLYQEWKQRR---YAFMRFLLER---YYGNKRGIEKIAPIEVQTED 233 Query: 239 PLDDILRLFKRNQYHFI---------YVMNEDKSIVAVVPEQKLISS 276 L I F+R H I Y ++E++ + A E++ SS Sbjct: 234 RLYTIFTKFRRGYKHSIIVRGKYKEHYTLDENELLYAYFTEKRTTSS 280 >ref|NP_658478.1| S2Pmetalloprt, S2P metal dependent membrane associated protease domain [Bacillus anthracis A2012] ref|NP_846892.1| stage IV sporulation protein FB [Bacillus anthracis str. Ames] gb|AAP28378.1| stage IV sporulation protein FB [Bacillus anthracis str. Ames] Length = 286 Score = 132 bits (333), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 87/290 (30%), Positives = 142/290 (48%), Gaps = 24/290 (8%) Query: 1 LIRIRGI--QLALHPLFVLIMLLSVVTGQFLELLTLFTIVFIHEMGHVCAALLCGVQVKS 58 +I+ R + ++++HPLF +I+++ + T +F ELL LF IV IHE+GH AA ++K Sbjct: 1 MIKYREVLTKISVHPLFWVIIVIGIFTARFKELLLLFCIVLIHELGHAFAAAHYNWRIKK 60 Query: 59 VQLLPFGGVAVMEDHGRLTAVREMGIAIAGPLQNVLLIVMAYGLRQTGYGTSAFLDYFIQ 118 +QLLPFGGVA +E+HG + E + IAGP+Q++ ++ + Y L + G+ + +F+ Sbjct: 61 IQLLPFGGVAELEEHGNKSLKEEFVVVIAGPIQHIWMMAIGYMLFEAGWLHAELYYFFMW 120 Query: 119 ANAIIALFNLLPVLPLDGGKIFQAMXXXXXXXXXXXXWCGRVSIAASVLVVGYAMXXXXX 178 N II FNLLP+ PLDGGK+ + ++S +++G+ + Sbjct: 121 NNIIILAFNLLPIWPLDGGKVLFNVLSYRFPYLQAHEKMMKLSCVFFSVILGWQLLWNSN 180 Query: 179 XXXXXXXXXMIGAFLLYSNLTDQRNLPYRFVSFLMNREAVYERYQQSGSAARPIVAFSAK 238 + A LY +R Y F+ FL+ ERY + I K Sbjct: 181 NIMMWVLLIFL-AVSLYQEWKQRR---YAFMRFLL------ERYYGNKRGIEKIAPIEVK 230 Query: 239 PLD---DILRLFKRNQYHFI---------YVMNEDKSIVAVVPEQKLISS 276 D I F+R H I Y ++E++ + A E++ SS Sbjct: 231 TEDRLYTIFTKFRRGYKHSIIVHGKYKEHYTLDENELLYAYFTEKRTTSS 280 >ref|NP_980831.1| stage IV sporulation protein FB [Bacillus cereus ATCC 10987] gb|AAS43439.1| stage IV sporulation protein FB [Bacillus cereus ATCC 10987] Length = 286 Score = 131 bits (330), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 86/291 (29%), Positives = 143/291 (49%), Gaps = 26/291 (8%) Query: 1 LIRIRGI--QLALHPLFVLIMLLSVVTGQFLELLTLFTIVFIHEMGHVCAALLCGVQVKS 58 +I+ R + ++++HPLF +I+++ + T +F ELL LF IV IHE+GH AA ++K Sbjct: 1 MIKYRDVLTKISVHPLFWVIIVIGIFTARFKELLLLFCIVLIHELGHAFAAAHYNWRIKK 60 Query: 59 VQLLPFGGVAVMEDHGRLTAVREMGIAIAGPLQNVLLIVMAYGLRQTGYGTSAFLDYFIQ 118 +QLLPFGGVA +E+HG + E+ + IAGP+Q++ ++ + Y L + G+ + +F+ Sbjct: 61 IQLLPFGGVAELEEHGNKSLKEELVVVIAGPIQHIWMMAVGYMLFEAGWLHAELYYFFMW 120 Query: 119 ANAIIALFNLLPVLPLDGGKI-FQAMXXXXXXXXXXXXWCGRVSIAASVLVVGYAMXXXX 177 N II FNLLP+ PLDGGK+ F + + SV++ G + Sbjct: 121 NNIIILAFNLLPIWPLDGGKVLFNVLSYRFPYLQAHEKMMKLSCVFFSVILGGQLLWNSN 180 Query: 178 XXXXXXXXXXMIGAFLLYSNLTDQRNLPYRFVSFLMNREAVYERYQQSGSAARPIVAFSA 237 + A LY +R Y F+ FL+ ERY + I Sbjct: 181 NIMMWVLLIFL--AVSLYQEWKQRR---YAFMRFLL------ERYYGNKRGIEKIAPIEV 229 Query: 238 KPLDDILRLFK------------RNQYHFIYVMNEDKSIVAVVPEQKLISS 276 + D + ++F R +Y Y ++E++ + A E++ SS Sbjct: 230 QSEDHLYKIFTKFRRGYKHSIIVRGKYKEHYTLDENELLYAYFTEKRTTSS 280 >ref|ZP_00097333.1| COG1994: Zn-dependent proteases [Desulfitobacterium hafniense] Length = 291 Score = 105 bits (262), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 58/141 (41%), Positives = 86/141 (60%) Query: 3 RIRGIQLALHPLFVLIMLLSVVTGQFLELLTLFTIVFIHEMGHVCAALLCGVQVKSVQLL 62 RI+G+ + +HP F L++L V G F + L +F +V HEM H+ A G +V ++L Sbjct: 5 RIQGLSIKVHPTFWLVLLAYGVLGLFTQALLIFLLVMGHEMAHLLTAKAYGFRVNGLELY 64 Query: 63 PFGGVAVMEDHGRLTAVREMGIAIAGPLQNVLLIVMAYGLRQTGYGTSAFLDYFIQANAI 122 PFGGVA ED + E +A+AGP+ N++L+ A LR G T + F+Q N Sbjct: 65 PFGGVAHCEDLFEGRRLEESMMALAGPVFNLILLFGAQALRWEGIWTGPTAEAFVQLNFW 124 Query: 123 IALFNLLPVLPLDGGKIFQAM 143 +A+FNL PVLPLDGG++ +A+ Sbjct: 125 LAVFNLTPVLPLDGGRVVRAL 145 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.331 0.144 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 325,392,871 Number of extensions: 10914512 Number of successful extensions: 33475 Number of sequences better than 1.0e-05: 63 Number of HSP's gapped: 33452 Number of HSP's successfully gapped: 63 Length of query: 280 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 156 Effective length of database: 420,565,302 Effective search space: 65608187112 Effective search space used: 65608187112 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 125 (52.8 bits)