BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0018.p (427 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00059803.1| COG1530: Ribonucleases G and E [Clostridium t... 328 2e-88 ref|NP_954280.1| ribonuclease G [Geobacter sulfurreducens PCA] >... 315 1e-84 ref|NP_253167.1| cytoplasmic axial filament protein [Pseudomonas... 310 5e-83 gb|AAR37494.1| cytoplasmic axial filament protein [uncultured ba... 309 7e-83 gb|EAJ72874.1| unknown [environmental sequence] 307 3e-82 >ref|ZP_00059803.1| COG1530: Ribonucleases G and E [Clostridium thermocellum ATCC 27405] Length = 497 Score = 328 bits (840), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 179/416 (43%), Positives = 254/416 (61%), Gaps = 9/416 (2%) Query: 15 MKQMIVHCMQHVTRMALLDNGSLVEYAAERDQQQGLVGSYYKGRVMNVLPGMQAAFVDIG 74 + +++V + R+ALL++ LVE ER+ + LVG+ Y+GRV +VLPGMQAAF+DIG Sbjct: 2 VSEIVVDVGINEKRVALLEDRELVELFIERNDCERLVGNIYRGRVESVLPGMQAAFIDIG 61 Query: 75 QKKNAFLYVDDVLHPHLERQ-------PEVKP-SIETLLQPGQEIVVQVRKEPRGGKGAR 126 +KNAFLYV D + P E +VK +IE +L+PGQEI VQV KEP G KG R Sbjct: 62 YEKNAFLYVGDAI-PQKEFSEDDEEIYSDVKSYNIEEILRPGQEITVQVTKEPIGTKGPR 120 Query: 127 VTTHYTLPGRWMVYMPFADYVGVSKKISRESERARLKAIGERLRRQEEGIIMRTVSEDEP 186 VTTH TLPGR MV +P ADY+G+S++I E ERA+L+ I E+++ + GII+RTVSE + Sbjct: 121 VTTHITLPGRQMVLLPNADYIGISRRIEDEEERAKLRKIAEKIKPKNMGIIVRTVSEGKR 180 Query: 187 VEAVEGDLSFLRSQWEMITRRAQECGAPALLHSDLSIVQRFIRDAFNPQQDELMIDSAKA 246 E + DL+FL W I +R Q P LH DLS++ R +RD F D +I+ + Sbjct: 181 EEDFKSDLNFLVKLWAKIKQREQSGPVPRCLHKDLSVIYRAVRDIFTWNIDRFVINDRQE 240 Query: 247 VREAEAFLNDMAPGKYKPVRFYHGQESIFTAYGVQEQLHKSFGRKITLEGGATLIWDETE 306 + + ++P V +++ +F Y + + K+ +K+ L+ G ++ + TE Sbjct: 241 YNKVLELVEMISPALKMRVEYFNKNIDLFEYYQIDSMIQKALAKKVWLKCGGYIVIERTE 300 Query: 307 ALTVIDVNTAQYTGGTSLEETVTRTNLLAAEEIGRLIRLRDTXXXXXXXXXXMQREEHRK 366 ALTVIDVNT +Y G +LE+TV RTNL A +EIG+ +RLRD M EH+K Sbjct: 301 ALTVIDVNTGKYVGVNNLEDTVLRTNLDAVKEIGKQLRLRDIGGIIIIDFIDMHDPEHQK 360 Query: 367 MVTERLERVISRDRTQTHILGWTHLGLLEMTRKKARHDSAGFAPAICQCCGGTGKV 422 V E L++V+ +DRT+T ++G T LGL+EMTRKK R C C G GK+ Sbjct: 361 QVLEALKQVLKKDRTKTTVVGMTGLGLIEMTRKKVREGLESMMLQDCPYCEGRGKI 416 >ref|NP_954280.1| ribonuclease G [Geobacter sulfurreducens PCA] gb|AAR36630.1| ribonuclease G [Geobacter sulfurreducens PCA] Length = 531 Score = 315 bits (808), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 169/425 (39%), Positives = 253/425 (59%), Gaps = 16/425 (3%) Query: 16 KQMIVHCMQHVTRMALLDNGSLVEYAAERDQQQGLVGSYYKGRVMNVLPGMQAAFVDIGQ 75 +++++ H TR+AL++NG++ E ER +++G+VG+ YKGRV+ VLPGMQAAFVDIG Sbjct: 34 NELVINTSSHETRIALIENGTIAELYIERSREKGIVGNIYKGRVVRVLPGMQAAFVDIGL 93 Query: 76 KKNAFLYVDDVL------HPHLERQPEVKPS----------IETLLQPGQEIVVQVRKEP 119 +K AFLYV DV +E E S IE LLQ GQEI+VQV KEP Sbjct: 94 EKAAFLYVADVFDAMEEFETFMEVNGEESDSERQVLHPLHPIEELLQEGQEILVQVSKEP 153 Query: 120 RGGKGARVTTHYTLPGRWMVYMPFADYVGVSKKISRESERARLKAIGERLRRQEEGIIMR 179 G KGAR+T+H +LPGR +VYMP D+VGVS++I E ER RLK I + + G I+R Sbjct: 154 IGTKGARITSHVSLPGRHLVYMPTVDHVGVSRRIEDEPERERLKEIVGKAKPAGGGFIVR 213 Query: 180 TVSEDEPVEAVEGDLSFLRSQWEMITRRAQECGAPALLHSDLSIVQRFIRDAFNPQQDEL 239 T +E + E + D+++L WE + RR ++ AP L+H+DL + Q+ IRD + Sbjct: 214 TAAEGKTEEDLVADMNYLTKLWEEVVRRNEKAHAPCLIHADLDVTQKVIRDILTENVSRI 273 Query: 240 MIDSAKAVREAEAFLNDMAPGKYKPVRFYHGQESIFTAYGVQEQLHKSFGRKITLEGGAT 299 ++DS + F++ P + Y +E IF +G++ ++ ++ GRK+ L+ G Sbjct: 274 VVDSRHEHDKIVQFISTFMPKMKYSMELYDDEEPIFDHFGLEVEISRALGRKVWLKSGGY 333 Query: 300 LIWDETEALTVIDVNTAQYTGGTSLEETVTRTNLLAAEEIGRLIRLRDTXXXXXXXXXXM 359 +I ++TEALT IDVNT ++ G +LE+T+ +TNL A +EI +RLR+ M Sbjct: 334 IIIEQTEALTAIDVNTGRFVGKHNLEDTILKTNLEAVKEIAYQLRLRNIGGIIIIDFIDM 393 Query: 360 QREEHRKMVTERLERVISRDRTQTHILGWTHLGLLEMTRKKARHDSAGFAPAICQCCGGT 419 ++E +R+ V LE + D+++T+IL + LGL+EMTRK+ R C C G Sbjct: 394 EKEVNREKVFTALEEAMKADKSKTNILKISDLGLVEMTRKRVRESIGRMMCEPCPYCEGR 453 Query: 420 GKVGA 424 G V + Sbjct: 454 GYVKS 458 >ref|NP_253167.1| cytoplasmic axial filament protein [Pseudomonas aeruginosa PA01] pir||G83086 cytoplasmic axial filament protein PA4477 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG07865.1| cytoplasmic axial filament protein [Pseudomonas aeruginosa PAO1] Length = 485 Score = 310 bits (794), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 163/411 (39%), Positives = 251/411 (61%), Gaps = 11/411 (2%) Query: 16 KQMIVHCMQHVTRMALLDNGSLVEYAAERDQQQGLVGSYYKGRVMNVLPGMQAAFVDIGQ 75 ++++++ +R+A+++NG L E ER Q++G+VG+ YKGRV+ VLPGMQAAFVDIG Sbjct: 3 EEILINITPMESRVAVVENGVLQEVHVERTQRRGIVGNIYKGRVVRVLPGMQAAFVDIGL 62 Query: 76 KKNAFLYVDDVLHPHLERQPEVKPSIETLLQPGQEIVVQVRKEPRGGKGARVTTHYTLPG 135 ++ AF++ ++ + R+ SI L+ GQ +VVQV K+P G KGAR+TTH ++P Sbjct: 63 ERAAFIHAAEISN----REGSAVESISALVHEGQALVVQVTKDPIGTKGARLTTHLSIPS 118 Query: 136 RWMVYMPFADYVGVSKKISRESERARLKAI------GERLRRQEEGIIMRTVSEDEPVEA 189 R++VYMP +VG+S +I E ER RLK + E + Q G I+RT +E + Sbjct: 119 RYLVYMPRTSHVGISLRIEDEVERERLKKVVADCVAAEGIEGQG-GFILRTAAEGAGEDE 177 Query: 190 VEGDLSFLRSQWEMITRRAQECGAPALLHSDLSIVQRFIRDAFNPQQDELMIDSAKAVRE 249 + D+ +LR W+ I + Q GAP++++ DLS+ R +RD NP+ +++ IDS + ++ Sbjct: 178 ILADIRYLRRLWDQIAAQIQTVGAPSVIYEDLSLAIRTLRDLVNPRIEKIRIDSRENFQK 237 Query: 250 AEAFLNDMAPGKYKPVRFYHGQESIFTAYGVQEQLHKSFGRKITLEGGATLIWDETEALT 309 +F+ ++ P + Y G+ IF YGV++++ K+ RK+ L+ G LI D TEA+T Sbjct: 238 ITSFVEELMPEISDRLEHYPGERPIFDLYGVEDEIQKALERKVLLKSGGYLIVDPTEAMT 297 Query: 310 VIDVNTAQYTGGTSLEETVTRTNLLAAEEIGRLIRLRDTXXXXXXXXXXMQREEHRKMVT 369 IDVNT + G +LEET+ +TNL AA I R +RLR+ M+ EEHR+ V Sbjct: 298 TIDVNTGAFVGHRNLEETIFKTNLEAATAIARQLRLRNLGGIIIIDFIDMEDEEHRRQVL 357 Query: 370 ERLERVISRDRTQTHILGWTHLGLLEMTRKKARHDSAGFAPAICQCCGGTG 420 LE+ + RD +T+I+G T LGL++MTRK+ R C CC G G Sbjct: 358 RTLEKQLERDHAKTNIIGITELGLVQMTRKRTRESLVQILCEPCPCCQGRG 408 >gb|AAR37494.1| cytoplasmic axial filament protein [uncultured bacterium 106] Length = 489 Score = 309 bits (792), Expect = 7e-83, Method: Compositional matrix adjust. Identities = 159/414 (38%), Positives = 257/414 (62%), Gaps = 5/414 (1%) Query: 16 KQMIVHCMQHVTRMALLDNGSLVEYAAERDQQQGLVGSYYKGRVMNVLPGMQAAFVDIGQ 75 K++I++ H TR+A+++ G L E ER++ LV + YKG+V+NVLPGM++AFVDIG Sbjct: 3 KEIIINHTSHETRVAVIEGGELSELYHEREKTIRLVDNIYKGKVLNVLPGMESAFVDIGS 62 Query: 76 KKNAFLYVDDVLHPH-LERQPEV----KPSIETLLQPGQEIVVQVRKEPRGGKGARVTTH 130 +K AFL++DD+L H + +P + I L+Q GQ+++VQV K P G KGAR+T+H Sbjct: 63 EKAAFLHIDDILPEHEIFGEPSNNNGEQQKIGQLIQEGQDVLVQVSKGPIGTKGARITSH 122 Query: 131 YTLPGRWMVYMPFADYVGVSKKISRESERARLKAIGERLRRQEEGIIMRTVSEDEPVEAV 190 ++PGR +VY+P +GVS++I++E ER RLK I +R+R + G I+RTV+E E + Sbjct: 123 VSIPGRNLVYLPATKTLGVSRQINQEKERERLKEIVDRIRPENAGFIIRTVAEGHSEEDL 182 Query: 191 EGDLSFLRSQWEMITRRAQECGAPALLHSDLSIVQRFIRDAFNPQQDELMIDSAKAVREA 250 D+++L S W+ I + + AP+LLHSDL+++ R IRD F+ +L++D + Sbjct: 183 HSDINYLVSIWDDILEKYKTLPAPSLLHSDLNVIFRTIRDLFSADIRKLVVDDKSQFNKV 242 Query: 251 EAFLNDMAPGKYKPVRFYHGQESIFTAYGVQEQLHKSFGRKITLEGGATLIWDETEALTV 310 F+ P + Y E +F YG++ ++ ++ G K+ L G L+ D+TEALT Sbjct: 243 RQFVRSYLPRYGSVIENYTKAEPVFDHYGIENEIDRALGNKVWLRSGGHLVIDQTEALTA 302 Query: 311 IDVNTAQYTGGTSLEETVTRTNLLAAEEIGRLIRLRDTXXXXXXXXXXMQREEHRKMVTE 370 +DVNT ++ G S E+T+ RTN+ AAEEI ++LR+ M+ ++HR+MV + Sbjct: 303 VDVNTGRFVGKESHEKTILRTNIEAAEEIVYQLQLRNIGGIIIIDFIDMETQQHRQMVYQ 362 Query: 371 RLERVISRDRTQTHILGWTHLGLLEMTRKKARHDSAGFAPAICQCCGGTGKVGA 424 L+ + D+ ++ IL + +GL+EMTRK+ R + + C C GTG++ + Sbjct: 363 TLKDKLRSDKARSKILQISEMGLVEMTRKRDRENLNRYLCDPCPYCDGTGQIKS 416 >gb|EAJ72874.1| unknown [environmental sequence] Length = 490 Score = 307 bits (787), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 159/420 (37%), Positives = 263/420 (62%), Gaps = 16/420 (3%) Query: 16 KQMIVHCMQHVTRMALLDNGSLVEYAAERDQQQGLVGSYYKGRVMNVLPGMQAAFVDIGQ 75 K++I++ H TR+AL+++G L E ER+++Q +VG+ YKGRV+ VLPGM++AF+DIG Sbjct: 3 KEIIINHSHHETRVALMEDGMLTELQYEREREQRIVGNIYKGRVVKVLPGMESAFIDIGA 62 Query: 76 KKNAFLYVDDVL-----------HPHLERQPEVKPSIETLLQPGQEIVVQVRKEPRGGKG 124 ++ AFL++DD++ + E+QP I+ LL+ G I+VQV K P G KG Sbjct: 63 ERAAFLHIDDIIPEEEMDGQGKRNSRKEKQP-----IDKLLKEGNPILVQVSKGPIGTKG 117 Query: 125 ARVTTHYTLPGRWMVYMPFADYVGVSKKISRESERARLKAIGERLRRQEEGIIMRTVSED 184 AR+T H ++PGR +VY+P + +GVS++I+ E ER RLK I RL+ ++ G I+RTV+E+ Sbjct: 118 ARITGHVSMPGRNLVYIPGSKTLGVSRQIADERERDRLKNIVNRLKPEDAGFIIRTVAEN 177 Query: 185 EPVEAVEGDLSFLRSQWEMITRRAQECGAPALLHSDLSIVQRFIRDAFNPQQDELMIDSA 244 + + D+++L S WE I + Q AP+LLHSDL+++ R +RD F+ +L++D Sbjct: 178 RSEDDLHSDINYLISLWEDIREKYQTQEAPSLLHSDLNVIFRTLRDLFSSDIKKLVVDHK 237 Query: 245 KAVREAEAFLNDMAPGKYKPVRFYHGQESIFTAYGVQEQLHKSFGRKITLEGGATLIWDE 304 + +E + F+ P V Y ++ IF Y ++ ++ ++ K+ L G +L+ ++ Sbjct: 238 EEYKEIKNFVRSYLPRYGSVVEHYSRRDPIFDHYNIEMEIERALSPKVWLRSGGSLVINQ 297 Query: 305 TEALTVIDVNTAQYTGGTSLEETVTRTNLLAAEEIGRLIRLRDTXXXXXXXXXXMQREEH 364 TEALT IDVNT ++ G S E+T+ RTNL AAEE+ ++LR+ M+ ++H Sbjct: 298 TEALTAIDVNTGRFVGKESHEKTILRTNLEAAEEVVYQLQLRNIGGIIIIDFIDMEVQQH 357 Query: 365 RKMVTERLERVISRDRTQTHILGWTHLGLLEMTRKKARHDSAGFAPAICQCCGGTGKVGA 424 R+MV + L+ + D+ ++ IL + +GL+EMTRK+ R + + + C C GTG++ + Sbjct: 358 RQMVYQALKDHLRNDKARSKILQISEMGLVEMTRKRDRENLSRYLCDPCPYCEGTGQIKS 417 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 598,208,542 Number of extensions: 23457578 Number of successful extensions: 57552 Number of sequences better than 1.0e-05: 764 Number of HSP's gapped: 56603 Number of HSP's successfully gapped: 767 Length of query: 427 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 299 Effective length of database: 409,704,906 Effective search space: 122501766894 Effective search space used: 122501766894 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)