BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0015.p (473 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_647136.1| respiratory nitrate reductase alpha chain [Stap... 775 0.0 ref|NP_372921.1| respiratory nitrate reductase alpha chain [Stap... 775 0.0 ref|NP_765530.1| respiratory nitrate reductase alpha chain [Stap... 766 0.0 gb|AAC82542.1| NarG [Staphylococcus carnosus] 747 0.0 ref|NP_785105.1| nitrate reductase, alpha chain [Lactobacillus p... 633 e-180 >ref|NP_647136.1| respiratory nitrate reductase alpha chain [Staphylococcus aureus subsp. aureus MW2] dbj|BAB96184.1| respiratory nitrate reductase alpha chain [Staphylococcus aureus subsp. aureus MW2] Length = 1229 Score = 775 bits (2000), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/476 (75%), Positives = 419/476 (88%), Gaps = 5/476 (1%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 +V+LDFRMT TPLY+DIVLPAATWYEK DLSSTDMHP+VHPFNPA++PLWESRSDWDIY+ Sbjct: 756 VVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFNPAIDPLWESRSDWDIYK 815 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 LA+ FSEMAK LPG +KD+V +PL+HD+ EIS P G+VKDW+KGE+EAVPG+TMPN Sbjct: 816 TLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVVKDWSKGEIEAVPGRTMPNF 875 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYD---ESIKNG 177 +IVERDYTKI+DKY++LGP L GK GAHG+SF V+E+YEELK + G + D +S++ Sbjct: 876 AIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQYEELKSMLGTWSDTNDDSVRAN 935 Query: 178 LPKIQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITA 237 P+I TAR VAD IL +SSATNG++SQK+YE E+ +G+ L+DIS++RAAEKI+F +IT+ Sbjct: 936 RPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQTGMPLKDISSERAAEKISFLNITS 995 Query: 238 QPREVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVY 297 QPREVIPT VF K+ RRYSPFTTNIERLVPFRTLTGRQ +Y+DHE+FQQFGE+LPVY Sbjct: 996 QPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFRTLTGRQSYYVDHEVFQQFGESLPVY 1055 Query: 298 KPTLPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXI 357 KPTLPPMVFG RD+++KGG DALVLRYLTPHGKWNIHS YQDN+HMLTLFRGGPT I Sbjct: 1056 KPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGKWNIHSMYQDNKHMLTLFRGGPT--VWI 1113 Query: 358 NNEDAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDT 417 +NEDAE+H I DNDWLEVYNRNGVVTARAV+SHRMP+GTMFMYHAQDKHIQ PGSEITDT Sbjct: 1114 SNEDAEKHDIQDNDWLEVYNRNGVVTARAVISHRMPKGTMFMYHAQDKHIQTPGSEITDT 1173 Query: 418 RGGSHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLED 473 RGGSHNAPTRIHLKPTQ+VGGYAQ+SY FNYYGPIGNQRD+YVAVR KEVNWLED Sbjct: 1174 RGGSHNAPTRIHLKPTQLVGGYAQISYHFNYYGPIGNQRDLYVAVRKMKEVNWLED 1229 >ref|NP_372921.1| respiratory nitrate reductase alpha chain [Staphylococcus aureus subsp. aureus Mu50] ref|NP_375508.1| respiratory nitrate reductase alpha chain [Staphylococcus aureus subsp. aureus N315] pir||F90040 respiratory nitrate reductase alpha chain [imported] - Staphylococcus aureus (strain N315) dbj|BAB43487.1| respiratory nitrate reductase alpha chain [Staphylococcus aureus subsp. aureus N315] dbj|BAB58559.1| respiratory nitrate reductase alpha chain [Staphylococcus aureus subsp. aureus Mu50] Length = 1229 Score = 775 bits (2000), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/476 (75%), Positives = 419/476 (88%), Gaps = 5/476 (1%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 +V+LDFRMT TPLY+DIVLPAATWYEK DLSSTDMHP+VHPFNPA++PLWESRSDWDIY+ Sbjct: 756 VVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFNPAIDPLWESRSDWDIYK 815 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 LA+ FSEMAK LPG +KD+V +PL+HD+ EIS P G+VKDW+KGE+EAVPG+TMPN Sbjct: 816 TLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVVKDWSKGEIEAVPGRTMPNF 875 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYD---ESIKNG 177 +IVERDYTKI+DKY++LGP L GK GAHG+SF V+E+YEELK + G + D +S++ Sbjct: 876 AIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQYEELKSMLGTWSDTNDDSVRAN 935 Query: 178 LPKIQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITA 237 P+I TAR VAD IL +SSATNG++SQK+YE E+ +G+ L+DIS++RAAEKI+F +IT+ Sbjct: 936 RPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQTGMPLKDISSERAAEKISFLNITS 995 Query: 238 QPREVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVY 297 QPREVIPT VF K+ RRYSPFTTNIERLVPFRTLTGRQ +Y+DHE+FQQFGE+LPVY Sbjct: 996 QPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFRTLTGRQSYYVDHEVFQQFGESLPVY 1055 Query: 298 KPTLPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXI 357 KPTLPPMVFG RD+++KGG DALVLRYLTPHGKWNIHS YQDN+HMLTLFRGGPT I Sbjct: 1056 KPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGKWNIHSMYQDNKHMLTLFRGGPT--VWI 1113 Query: 358 NNEDAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDT 417 +NEDAE+H I DNDWLEVYNRNGVVTARAV+SHRMP+GTMFMYHAQDKHIQ PGSEITDT Sbjct: 1114 SNEDAEKHDIQDNDWLEVYNRNGVVTARAVISHRMPKGTMFMYHAQDKHIQTPGSEITDT 1173 Query: 418 RGGSHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLED 473 RGGSHNAPTRIHLKPTQ+VGGYAQ+SY FNYYGPIGNQRD+YVAVR KEVNWLED Sbjct: 1174 RGGSHNAPTRIHLKPTQLVGGYAQISYHFNYYGPIGNQRDLYVAVRKMKEVNWLED 1229 >ref|NP_765530.1| respiratory nitrate reductase alpha chain [Staphylococcus epidermidis ATCC 12228] gb|AAO05616.1| respiratory nitrate reductase alpha chain [Staphylococcus epidermidis ATCC 12228] Length = 1227 Score = 766 bits (1979), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/476 (75%), Positives = 413/476 (86%), Gaps = 5/476 (1%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 +V+LDFRMT TPLY+DIVLPAATWYEK DLSSTDMHPF+HPFNPA++PLWESRSDWDIY+ Sbjct: 754 LVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPFIHPFNPAIDPLWESRSDWDIYK 813 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 L++ SEMAK LPG +KD+V +PL HDS EIS G+VKDW+KGE+E VPGKTMPN Sbjct: 814 TLSKAVSEMAKDYLPGKFKDVVTTPLGHDSKQEISTEYGIVKDWSKGEIEGVPGKTMPNF 873 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYDE---SIKNG 177 SIVERDYT+I+DK++++GP L GK GAHG+S+SV+EEYEELK + G + D+ S+KN Sbjct: 874 SIVERDYTQIYDKFVTVGPKLEKGKIGAHGVSYSVSEEYEELKSIVGTWNDDNTISVKND 933 Query: 178 LPKIQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITA 237 P+I TAR+VAD IL++SSATNG++SQK+YE E +G+ L+DIS +RA+EKI+F +IT+ Sbjct: 934 RPRIDTARKVADVILNISSATNGKLSQKSYEDLENQTGMELKDISKERASEKISFLNITS 993 Query: 238 QPREVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVY 297 QPREVIPT VF K RRYSPFTTN+ERLVPFRTLTGRQ +YIDHE+FQQFGE+LPVY Sbjct: 994 QPREVIPTAVFPGSNKDGRRYSPFTTNVERLVPFRTLTGRQSYYIDHEVFQQFGESLPVY 1053 Query: 298 KPTLPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXI 357 KPTLPPMVFG RD++VKGGQD LVLRYLTPHGKWNIHSTYQDN+ MLTLFRGGP I Sbjct: 1054 KPTLPPMVFGARDKKVKGGQDTLVLRYLTPHGKWNIHSTYQDNERMLTLFRGGPV--VWI 1111 Query: 358 NNEDAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDT 417 +NEDA HGI+DNDWLEVYNRNGVVTARAV SHRMPRGTMFMYHAQDKHI+ PGSEITDT Sbjct: 1112 SNEDAADHGINDNDWLEVYNRNGVVTARAVTSHRMPRGTMFMYHAQDKHIETPGSEITDT 1171 Query: 418 RGGSHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLED 473 RGGSHNAPTRIHLKPTQ+VGGYAQ+SY FNYYGPIGNQRD YVAVR KEVNWLED Sbjct: 1172 RGGSHNAPTRIHLKPTQLVGGYAQISYHFNYYGPIGNQRDEYVAVRKMKEVNWLED 1227 >gb|AAC82542.1| NarG [Staphylococcus carnosus] Length = 1224 Score = 747 bits (1929), Expect = 0.0, Method: Compositional matrix adjust. Identities = 351/473 (74%), Positives = 402/473 (84%), Gaps = 2/473 (0%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 +VALDFRMT+TPL ADIVLPAATWYEK DLSSTDMHPFVHPFNPA++PLWESRSDWDI++ Sbjct: 754 IVALDFRMTSTPLKADIVLPAATWYEKHDLSSTDMHPFVHPFNPAIDPLWESRSDWDIFK 813 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 L++ SEMAK +PG YKD+V SPLAHDS EI+ P G VKDW KGEVEA+PGKTMP Sbjct: 814 SLSQTVSEMAKVYMPGTYKDVVTSPLAHDSKQEIATPYGKVKDWTKGEVEAIPGKTMPGF 873 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYDESIKNGLPK 180 ++V+RDYT I+DKY++ GP L K GAHG+SF V ++YEE K + G + D +IK+G P+ Sbjct: 874 AVVDRDYTLIYDKYVTAGPLLETAKLGAHGVSFPVKKQYEEAKDIVGTWSDGTIKDGRPR 933 Query: 181 IQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITAQPR 240 I TAR+ AD +L++SSATNG +SQ +Y+ E+ +G+ L DIS+DR+AEKITF +IT+QPR Sbjct: 934 IDTARRAADVVLNISSATNGELSQASYKDLEEKTGMPLTDISSDRSAEKITFMNITSQPR 993 Query: 241 EVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVYKPT 300 EVIPT VF K +RYSPFTTNIERLVPFRTLTGRQ +Y+DHEIFQQFGE+LPVYKPT Sbjct: 994 EVIPTAVFSGSNKGNKRYSPFTTNIERLVPFRTLTGRQSYYLDHEIFQQFGESLPVYKPT 1053 Query: 301 LPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXINNE 360 LPPMVFG +D++V GGQD LVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPT I E Sbjct: 1054 LPPMVFGTKDKQVIGGQDNLVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPT--VWIAKE 1111 Query: 361 DAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDTRGG 420 DAE H I DNDWLEVYNRNGVVTARAV SHRMP+GTMFMYHAQDKHIQ PGSEIT RGG Sbjct: 1112 DAEAHDIHDNDWLEVYNRNGVVTARAVTSHRMPKGTMFMYHAQDKHIQTPGSEITGLRGG 1171 Query: 421 SHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLED 473 SHNAPTRIHLKPTQ+VGGYAQ+SY FNYYGPIGNQRDVYVAVR KEV+WLED Sbjct: 1172 SHNAPTRIHLKPTQLVGGYAQISYSFNYYGPIGNQRDVYVAVRKMKEVDWLED 1224 >ref|NP_785105.1| nitrate reductase, alpha chain [Lactobacillus plantarum WCFS1] emb|CAD63953.1| nitrate reductase, alpha chain [Lactobacillus plantarum WCFS1] Length = 1230 Score = 633 bits (1632), Expect = e-180, Method: Compositional matrix adjust. Identities = 296/473 (62%), Positives = 359/473 (75%), Gaps = 4/473 (0%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 ++ +DFRM TP+Y+D+VLPAATWYEK DLSSTDMHPF+HPFN A+NP+WES+SDW ++ Sbjct: 762 VIDMDFRMVATPMYSDVVLPAATWYEKADLSSTDMHPFIHPFNAAINPMWESKSDWQQFK 821 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 LA+ FS MAK P DL PL HDS EISQP+G ++DW KGE++A+PG+TMP L Sbjct: 822 TLAKTFSTMAKKYYPEPQYDLKTQPLGHDSKGEISQPLGEIRDWKKGEIDAIPGQTMPAL 881 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYDESIKNGLPK 180 S+++RDYT+++DKYI+LGPN GK G G S+ V EY+ELK + GV YD + G PK Sbjct: 882 SLIKRDYTQVYDKYIALGPN-ARGKEGGLGFSYDVTTEYDELKDILGV-YDRGVNAGCPK 939 Query: 181 IQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITAQPR 240 + ++V D ILH+SS +NG V++KA+ AE +G L DISA R E+++F+SIT QP Sbjct: 940 LDQDKRVVDAILHISSTSNGHVAKKAWANAETVTGRSLSDISAGRTEEQMSFKSITVQPA 999 Query: 241 EVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVYKPT 300 E IPTP+F RYSPFT NIERLVPFRTLTGRQ FY+DHEIFQ++GE+L YKPT Sbjct: 1000 EAIPTPIFTSAKHDGDRYSPFTVNIERLVPFRTLTGRQQFYLDHEIFQEYGESLANYKPT 1059 Query: 301 LPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXINNE 360 LPP V P D E+ + L LRY+TPHGKWNIH+ Y DN MLTLFRGGP ++ Sbjct: 1060 LPPQVMAPGDTEIAPADNELTLRYMTPHGKWNIHTMYYDNLEMLTLFRGGPN--VWLSPV 1117 Query: 361 DAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDTRGG 420 DA++ GI DNDWLE+YNRNGVVTARAVVSHRMP G M+MYHAQD IQ P S IT RGG Sbjct: 1118 DADKAGIKDNDWLEIYNRNGVVTARAVVSHRMPAGAMYMYHAQDMEIQEPLSTITGNRGG 1177 Query: 421 SHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLED 473 SHNAPT+IH+KPTQMVGGY QLSYGFNYYGPIGNQRD+YV VR K+VNW ED Sbjct: 1178 SHNAPTQIHVKPTQMVGGYGQLSYGFNYYGPIGNQRDLYVNVRKLKKVNWNED 1230 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 732,043,077 Number of extensions: 31221597 Number of successful extensions: 65111 Number of sequences better than 1.0e-05: 99 Number of HSP's gapped: 64987 Number of HSP's successfully gapped: 111 Length of query: 473 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 344 Effective length of database: 406,989,807 Effective search space: 140004493608 Effective search space used: 140004493608 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)