BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0001.p (287 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAB49832.1| gentamicin resistance protein 306 2e-82 ref|NP_655281.1| APH, Aminoglycoside phosphotransferase [Bacillu... 112 1e-23 ref|NP_977817.1| aminoglycoside phophotransferase family protein... 108 1e-22 ref|NP_978492.1| 6'-aminoglycoside N-acetyltransferase/2''-amino... 105 1e-21 ref|NP_831160.1| Gentamicin resistance protein [Bacillus cereus ... 104 2e-21 >gb|AAB49832.1| gentamicin resistance protein Length = 306 Score = 306 bits (785), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 150/246 (60%), Positives = 188/246 (76%), Gaps = 2/246 (0%) Query: 1 LKEQERRYSALIADQFPELNIRTVESLGEGYRNFAVLVNGEWVFRFPKSQQGADELNKEM 60 +K+ + Y+ +I QFP+++I++VESLGEG+RN+A+LVNG+WVFRFPKSQQGADELNKE+ Sbjct: 1 MKQNKLHYTTMIMTQFPDISIQSVESLGEGFRNYAILVNGDWVFRFPKSQQGADELNKEI 60 Query: 61 HLLPLLSERVKVGIPKFVYRGTQEDGRLFAGYRKVEGEILGEDGIASVSGDARERLAIQL 120 LLPLL VKV IP++VY G + DG F GYRKV+G+ILGEDG+A DA++RLA+QL Sbjct: 61 QLLPLLVGCVKVNIPQYVYIGKRSDGNPFVGYRKVQGQILGEDGMAVFPDDAKDRLALQL 120 Query: 121 GDFMSDLNAFPVETAIQAGVPVQQLADEIQLLKDAAERQVFPHLEPSSRGYLHRRFQAYL 180 +FM++L+AFPVETAI AGVPV L ++I LL +A E QVFP L+ S R YL RFQ+Y+ Sbjct: 121 AEFMNELSAFPVETAISAGVPVTNLKNKILLLSEAVEDQVFPLLDESLRDYLTLRFQSYM 180 Query: 181 ENTEYPRYIPALIHADLSPDHFLT--DSHRSVLTGIIDFGDAAISXXXXXXXXXXEDCGE 238 + Y RY P LIH DLSPDHFLT +S ++ LTGIIDFGDAAIS EDCGE Sbjct: 181 THPVYTRYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYLLEDCGE 240 Query: 239 PFTRQV 244 FTRQV Sbjct: 241 LFTRQV 246 >ref|NP_655281.1| APH, Aminoglycoside phosphotransferase [Bacillus anthracis A2012] ref|NP_843854.1| aminoglycoside phophotransferase family protein [Bacillus anthracis str. Ames] gb|AAP25340.1| aminoglycoside phophotransferase family protein [Bacillus anthracis str. Ames] Length = 300 Score = 112 bits (279), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 66/240 (27%), Positives = 118/240 (49%), Gaps = 3/240 (1%) Query: 8 YSALIADQFPELNIRTVESLGEGYRNFAVLVNGEWVFRFPKSQQGADELNKEMHLLPLLS 67 Y I + P L+I + + EG+ N AV+VN E +FRFP+ Q+ A + E L +L+ Sbjct: 4 YKQYIKEALPNLSIHSYKQNEEGWDNVAVIVNDELLFRFPRKQEYAMRIPLEKELCTILT 63 Query: 68 ERVK-VGIPKF--VYRGTQEDGRLFAGYRKVEGEILGEDGIASVSGDARERLAIQLGDFM 124 + ++ + +P++ +Y+ ++ L + Y + GE L + +A++ R+ + QL F+ Sbjct: 64 QSLQEIEVPQYHLIYKNESDEVPLCSYYTLIHGEPLKTEIVANLDEKERKIIITQLATFL 123 Query: 125 SDLNAFPVETAIQAGVPVQQLADEIQLLKDAAERQVFPHLEPSSRGYLHRRFQAYLENTE 184 + L++ P+++ G P ++ + L+ V L + L+R F+ + Sbjct: 124 AALHSIPLKSVTALGFPTEKTLTYWKELQTKLNEYVTNSLTSFQKSTLNRLFENFFACIA 183 Query: 185 YPRYIPALIHADLSPDHFLTDSHRSVLTGIIDFGDAAISXXXXXXXXXXEDCGEPFTRQV 244 + A+IHAD + H L D +++GIIDFGDA I D G FT V Sbjct: 184 TSAFPNAIIHADFTHHHILFDKQNKIISGIIDFGDAQIGDPAFDFAGLYYDFGHEFTTSV 243 >ref|NP_977817.1| aminoglycoside phophotransferase family protein [Bacillus cereus ATCC 10987] gb|AAS40425.1| aminoglycoside phophotransferase family protein [Bacillus cereus ATCC 10987] Length = 300 Score = 108 bits (270), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 66/240 (27%), Positives = 113/240 (47%), Gaps = 3/240 (1%) Query: 8 YSALIADQFPELNIRTVESLGEGYRNFAVLVNGEWVFRFPKSQQGADELNKEMHLLPLLS 67 Y I + P L+I + + EG+ N A++VN E +FRFP+ Q+ A + E L LLS Sbjct: 4 YKQYIKEALPNLSIHSYKQNEEGWDNVAIIVNDELLFRFPRKQEYAMRIPLEKELCTLLS 63 Query: 68 ERV-KVGIPKF--VYRGTQEDGRLFAGYRKVEGEILGEDGIASVSGDARERLAIQLGDFM 124 + ++ +PK+ Y + L + Y + GE L + + ++ R+ L QL F+ Sbjct: 64 CSLHEIEVPKYHLFYEKNTDAIPLCSYYTLIHGEPLKTEIVTTLEKQERKALITQLATFL 123 Query: 125 SDLNAFPVETAIQAGVPVQQLADEIQLLKDAAERQVFPHLEPSSRGYLHRRFQAYLENTE 184 + L++ P+++ G P+++ + L+ V L + L+R F+ + Sbjct: 124 AALHSIPLKSVTALGFPIEKTLTYWKELQAKLNEYVTNSLTSFQKSTLNRLFENFFACLA 183 Query: 185 YPRYIPALIHADLSPDHFLTDSHRSVLTGIIDFGDAAISXXXXXXXXXXEDCGEPFTRQV 244 ++ +IHAD + H L D ++G+IDFGDA I D G FT V Sbjct: 184 TSKFQNTIIHADFTHHHILFDKQNKTISGVIDFGDAQIGDPAFDFAGLYYDFGHEFTTSV 243 >ref|NP_978492.1| 6'-aminoglycoside N-acetyltransferase/2''-aminoglycoside phosphotransferase, putative [Bacillus cereus ATCC 10987] gb|AAS41100.1| 6'-aminoglycoside N-acetyltransferase/2''-aminoglycoside phosphotransferase, putative [Bacillus cereus ATCC 10987] Length = 293 Score = 105 bits (262), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 64/225 (28%), Positives = 115/225 (51%), Gaps = 8/225 (3%) Query: 4 QERRYSALIADQFPELNIRTVESLGEGYRNFAVLVNGEWVFRFPKSQQGADELNKEMHLL 63 +E Y A + +PEL I +V G N ++VN VFRFPK ++G +L E LL Sbjct: 3 KENEYVAYLQRLYPELQINSVYINEIGQNNDVLIVNDNIVFRFPKYEKGIQKLRIETQLL 62 Query: 64 PLLSERVKVGIPKFVYRGTQED--GRLFAGYRKVEGEILGEDGIASVSGDAR-ERLAIQL 120 + + + IP Y+G Q + G++FAGY +EG+ ++ ++ + + ++LA L Sbjct: 63 EKIRPFITLQIPNPSYQGFQNEVPGKVFAGYEMIEGDPFWKNVFTEINDEKQLQKLAYTL 122 Query: 121 GDFMSDLNAFPV---ETAIQAGVPVQQLADEIQLLKDAAERQVFPHLEPSSRGYLHRRFQ 177 F+ +L+ P+ E+ +Q + EI L + V+P + +R + F+ Sbjct: 123 ARFLKELHEIPLSTFESIMQCDST--DMYSEINSLYSQLKEHVYPFMRNVARKEVSTSFE 180 Query: 178 AYLENTEYPRYIPALIHADLSPDHFLTDSHRSVLTGIIDFGDAAI 222 YL + + + P+L+H D + L + + ++G+IDFG A+I Sbjct: 181 LYLNESSHFNFTPSLVHGDFGMTNILYSATKKNISGVIDFGGASI 225 >ref|NP_831160.1| Gentamicin resistance protein [Bacillus cereus ATCC 14579] gb|AAP08361.1| Gentamicin resistance protein [Bacillus cereus ATCC 14579] Length = 300 Score = 104 bits (259), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 61/240 (25%), Positives = 115/240 (47%), Gaps = 3/240 (1%) Query: 8 YSALIADQFPELNIRTVESLGEGYRNFAVLVNGEWVFRFPKSQQGADELNKEMHLLPLLS 67 Y I + P L+I + + EG+ N AV+VN E +FRFP+ Q+ A + E L +L+ Sbjct: 4 YKHYIKEALPNLSIHSYKQNEEGWDNVAVIVNDELLFRFPRKQEYAMRIPLEKELCAILT 63 Query: 68 ERVK-VGIPKF--VYRGTQEDGRLFAGYRKVEGEILGEDGIASVSGDARERLAIQLGDFM 124 + ++ + +P++ +Y+ ++ L + Y + GE L + +A++ + + QL F+ Sbjct: 64 QSLQEIEVPQYHLIYKNESDEVPLCSYYTLIHGEPLKTETVATLEDPKLKAIITQLATFL 123 Query: 125 SDLNAFPVETAIQAGVPVQQLADEIQLLKDAAERQVFPHLEPSSRGYLHRRFQAYLENTE 184 + L++ P+ P+++ + L+ + + L + L+R F+ + Sbjct: 124 AVLHSIPLHQVTTLCFPIEKTCTYWKELQTKLNQYLTTSLTSLQKLALNRLFENFFACLA 183 Query: 185 YPRYIPALIHADLSPDHFLTDSHRSVLTGIIDFGDAAISXXXXXXXXXXEDCGEPFTRQV 244 + +IHAD + H L + H ++G+IDFGDA I D G FT V Sbjct: 184 TSTFQNTIIHADFTHHHILFNKHNKSISGVIDFGDAQIGDPAFDFAGLYYDFGHEFTTSV 243 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.140 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 393,748,982 Number of extensions: 15576611 Number of successful extensions: 33544 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 33534 Number of HSP's successfully gapped: 13 Length of query: 287 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 163 Effective length of database: 420,565,302 Effective search space: 68552144226 Effective search space used: 68552144226 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)