BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0352.0014.p (432 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAR10426.1| TnpA [Enterococcus faecium] 450 e-125 ref|NP_688255.1| transposase, ISL3 family [Streptococcus agalact... 440 e-122 ref|NP_927597.1| Transposase TnpA, ISL3 family [Photorhabdus lum... 439 e-122 gb|AAL02177.1| transposase TnpA [Enterococcus faecium] 437 e-121 ref|NP_689008.1| transposase, ISL3 family [Streptococcus agalact... 428 e-118 >gb|AAR10426.1| TnpA [Enterococcus faecium] Length = 440 Score = 450 bits (1157), Expect = e-125, Method: Compositional matrix adjust. Identities = 232/440 (52%), Positives = 302/440 (68%), Gaps = 11/440 (2%) Query: 2 DILNLSKF-NILSISENEFDFLIQVVTSSPPLACPHCGCIANLYKHDSREQICMDLPIHG 60 D+L+L I S ENE D + +V PP CP CG LYKH SR Q+ MDLPI Sbjct: 3 DLLSLPDIKTIESPQENETDMMFKVEAVGPPERCPECG-FDKLYKHSSRNQLIMDLPIRL 61 Query: 61 KRVGLLIKRQRYRCRDCNRTFWEHLDHTIDEKRNCTKRLLSYIEKQSLQRTFVSISEDVG 120 KRVGL + R+RY+CR+C TFWE L ++DEKR+ TKRLL I++QS+ +TFV ++E VG Sbjct: 62 KRVGLQLNRRRYKCRECGSTFWERL-VSVDEKRSMTKRLLKSIQEQSMSKTFVEVAESVG 120 Query: 121 LHEKTIRNIFRDYINRLKETLRFETPNWLGIDEIHIIK-PRCVLTNIEERTLLDVLPNRN 179 + EKTIRN+F+DY+ + +FETP WLGIDEIHII+ PR VLTNIE RT+ D+ PNRN Sbjct: 121 VDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRPRLVLTNIERRTIYDIKPNRN 180 Query: 180 KETVVGYLSGLPNRGEIQYVTMNMWQPYKDAVRAILPKALIIVDKFHVVRMANQALETIR 239 KETV+ LS + +R I+YVTM+MW+PYKDAV ILP A ++VDKFHVVRMANQAL+ +R Sbjct: 181 KETVIQRLSEISDRTYIEYVTMDMWKPYKDAVNTILPHAKVVVDKFHVVRMANQALDNVR 240 Query: 240 KQLREGLSPKERRGLMHDRFILLKRHTALTEMDKITLDLWTKNHPSLGTAYDLKESFFDI 299 K L+ +S KERR LM +RFILLKR L E + L+ W N P+L AY+LKE F+ I Sbjct: 241 KSLKAHMSQKERRTLMRERFILLKRKHDLNERESFLLETWLGNLPALKEAYELKEEFYWI 300 Query: 300 WECDSRQTAFLKYNEWKAK-IPTELQSAFEPLTKAMKNWEEEIFAYFDHRITNAYMESLN 358 W+ + L+Y++W+ + + + + A++ L +A+ NW EIF YFD R+TNAY ES+N Sbjct: 301 WDTPNSDEGRLRYSQWRHRCMSSNSKDAYKDLVRAVDNWHVEIFNYFDKRLTNAYTESIN 360 Query: 359 SLIRVINRLGRGYSFEALRAKILFTEGLKKQRKPEENFPMFNLPPIGI------VREPEE 412 S+IR + R+GRGYSF+ALRAKILF E L K+RKP N FN + V + Sbjct: 361 SIIRQVERMGRGYSFDALRAKILFNEKLHKKRKPRFNSSAFNKAMLHDNFNWYEVNDHNI 420 Query: 413 IILPGIDFSTLIEKLEKGQL 432 G+DFSTLI+ LEKG L Sbjct: 421 TDNFGVDFSTLIKNLEKGDL 440 >ref|NP_688255.1| transposase, ISL3 family [Streptococcus agalactiae 2603V/R] gb|AAN00128.1| transposase, ISL3 family [Streptococcus agalactiae 2603V/R] Length = 435 Score = 440 bits (1131), Expect = e-122, Method: Compositional matrix adjust. Identities = 224/425 (52%), Positives = 292/425 (68%), Gaps = 15/425 (3%) Query: 16 ENEFDFLIQVVTSSPPLACPHCGCIANLYKHDSREQICMDLPIHGKRVGLLIKRQRYRCR 75 ENE D + +V PP CP CG LYKH SR Q+ MDLPI KRVGL + R+RY+CR Sbjct: 18 ENETDMMFKVEAVGPPERCPECG-FDKLYKHSSRNQLIMDLPIRLKRVGLHLNRRRYKCR 76 Query: 76 DCNRTFWEHLDHTIDEKRNCTKRLLSYIEKQSLQRTFVSISEDVGLHEKTIRNIFRDYIN 135 +C T ++DEKR+ TKRLL I++QS+ +TFV ++E VG+ EKTIRN+F+DY+ Sbjct: 77 ECGSTI------SVDEKRSMTKRLLKSIQEQSMSKTFVEVAESVGVDEKTIRNVFKDYVA 130 Query: 136 RLKETLRFETPNWLGIDEIHIIK-PRCVLTNIEERTLLDVLPNRNKETVVGYLSGLPNRG 194 + +FETP WLGIDEIHII+ PR VLTNIE RT+ D+ PNRNKETV+ LS + +R Sbjct: 131 LKEREYQFETPKWLGIDEIHIIRRPRLVLTNIERRTIYDIKPNRNKETVIQRLSEISDRT 190 Query: 195 EIQYVTMNMWQPYKDAVRAILPKALIIVDKFHVVRMANQALETIRKQLREGLSPKERRGL 254 I+YVTM+MW+PYKDAV ILP+A ++VDKFHVVRMANQAL+ +RK L+ +S KERR L Sbjct: 191 YIEYVTMDMWKPYKDAVNTILPQAKVVVDKFHVVRMANQALDNVRKSLKAHMSQKERRTL 250 Query: 255 MHDRFILLKRHTALTEMDKITLDLWTKNHPSLGTAYDLKESFFDIWECDSRQTAFLKYNE 314 M +RFILLKR L E + LD W N P+L AY+LKE F+ IW+ L+Y++ Sbjct: 251 MRERFILLKRKHDLNERESFLLDTWLGNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQ 310 Query: 315 WKAK-IPTELQSAFEPLTKAMKNWEEEIFAYFDHRITNAYMESLNSLIRVINRLGRGYSF 373 W+ + + + + A++ L +A+ NW EIF YFD R+TNAY ES+NS+IR + R+GRGYSF Sbjct: 311 WRHRCMSSNSKDAYKDLVRAVDNWHVEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSF 370 Query: 374 EALRAKILFTEGLKKQRKPEENFPMFNLPPIGI------VREPEEIILPGIDFSTLIEKL 427 +ALRAKILF E L K+RKP N FN + V + + G+DFSTLI+ L Sbjct: 371 DALRAKILFNEKLHKKRKPRFNSSAFNKAMLYDTFNWYEVNDHDITDNLGVDFSTLIKNL 430 Query: 428 EKGQL 432 EKG L Sbjct: 431 EKGDL 435 >ref|NP_927597.1| Transposase TnpA, ISL3 family [Photorhabdus luminescens subsp. laumondii TTO1] emb|CAE12529.1| Transposase TnpA, ISL3 family [Photorhabdus luminescens subsp. laumondii TTO1] Length = 429 Score = 439 bits (1128), Expect = e-122, Method: Compositional matrix adjust. Identities = 211/431 (48%), Positives = 301/431 (69%), Gaps = 4/431 (0%) Query: 2 DILNLSKFNILSISENEFDFLIQVVTSSPPLACPHCGCIANLYKHDSREQICMDLPIHGK 61 +ILN + +LS+SE E D+ + V T++PP C HC + R+++ MD P++GK Sbjct: 3 NILNWPDYIVLSVSELEHDYRVHVGTANPPTHCIHCKH-PEIVGFGRRDEVIMDTPVNGK 61 Query: 62 RVGLLIKRQRYRCRDCNRTFWEHLDHTIDEKRNCTKRLLSYIEKQSLQRTFVSISEDVGL 121 R G+++ R+RYRC+ C +TF E + H D KR T RL+ YIE++SL+RTF S++EDVG+ Sbjct: 62 RTGIILNRRRYRCQICCKTFMEPVPHK-DGKRQMTHRLIQYIERESLRRTFSSVAEDVGV 120 Query: 122 HEKTIRNIFRDYINRLKETLRFETPNWLGIDEIHIIKPRCVLTNIEERTLLDVLPNRNKE 181 EKT+RNIF D RL++T FE P WLGIDEIHIIKPRCV+ NIE +T++D+L N NK Sbjct: 121 DEKTVRNIFHDSCERLEKTFNFEMPKWLGIDEIHIIKPRCVIANIELQTIVDMLDNNNKA 180 Query: 182 TVVGYLSGLPNRGEIQYVTMNMWQPYKDAVRAILPKALIIVDKFHVVRMANQALETIRKQ 241 TV+ YL+ +R +++YV MNMW+PY++AV ++P A +I+DKFHVVRMAN++LE K Sbjct: 181 TVMRYLTKRKDRYQVRYVAMNMWRPYREAVATMIPDATVIIDKFHVVRMANESLERALKA 240 Query: 242 LREGLSPKERRGLMHDRFILLKRHTALTEMDKITLDLWTKNHPSLGTAYDLKESFFDIWE 301 +R GL+ ++RR LM + F+LLKR T+ + + W N+P LG AY+LKESFF IW+ Sbjct: 241 IRSGLTAQQRRRLMKNHFVLLKRRHEQTDAEYMRFSDWILNYPDLGYAYELKESFFGIWD 300 Query: 302 CDSRQTAFLKYNEWKAKIPTELQSAFEPLTKAMKNWEEEIFAYFDHRITNAYMESLNSLI 361 C +R A Y W +I E++ F+PL KA+ NW +++FAYFDH ITNAY+E+LN+LI Sbjct: 301 CKNRYEAQQAYYVWLRQITPEMKVYFDPLVKAIDNWHDDVFAYFDHPITNAYIENLNNLI 360 Query: 362 RVINRLGRGYSFEALRAKILFTEGLKKQRKPEENFPMFNLPPIGIVREPEEIILPGIDFS 421 RV+N +GRGYSFEALRA+ILFT+G ++ + P ++ +P E E I G+D S Sbjct: 361 RVVNHVGRGYSFEALRAEILFTKGFQQSKNP--HYQHQRIPEQACGPESETSINYGVDIS 418 Query: 422 TLIEKLEKGQL 432 L+ ++E G+L Sbjct: 419 ALVREIEMGRL 429 >gb|AAL02177.1| transposase TnpA [Enterococcus faecium] Length = 446 Score = 437 bits (1124), Expect = e-121, Method: Compositional matrix adjust. Identities = 222/447 (49%), Positives = 308/447 (68%), Gaps = 24/447 (5%) Query: 2 DILNLSKFNILSI--SENEFDFLIQVVTSSPPLACPHCGCIANLYKHDSREQICMDLPIH 59 ++LNLS F IL + SEN++ FL++ S PP C CG IANLYKH + Q+ DLP+H Sbjct: 4 NMLNLSNFKILDMQESENDYRFLVEP-KSPPPTRCQKCGTIANLYKHGKKRQLFFDLPMH 62 Query: 60 GKRVGLLIKRQRYRCRDCNRTFWEHLDHTIDEKRNCTKRLLSYIEKQSLQRTFVSISEDV 119 KRVG+ +KRQRY+CRDCN TF+E L +D+ R+ TKRL ++I++ SL++TF S++E++ Sbjct: 63 AKRVGIYLKRQRYKCRDCNETFFEKLPD-LDDARSVTKRLNNFIQEVSLEKTFTSVAEEI 121 Query: 120 GLHEKTIRNIFRDYINRLKETLRFETPNWLGIDEIHIIKP-RCVLTNIEERTLLDVLPNR 178 G+ EKT+RNIF DY+ L+ TP WLGIDE+H++K RCV+T+++ ++++D+L R Sbjct: 122 GVDEKTVRNIFNDYVAELESQTDLRTPKWLGIDEVHLLKNYRCVITDVKNKSVIDILRKR 181 Query: 179 NKETVVGYLSGLPNRGEIQYVTMNMWQPYKDAVRAILPKALIIVDKFHVVRMANQALETI 238 NK+TV+ YLS L + +I+ V M+MW PYK AV ++P A I++DKFHVV++AN+ALE I Sbjct: 182 NKDTVISYLSKLQDIDKIELVAMDMWNPYKSAVNTVIPHAKIVIDKFHVVKLANEALEKI 241 Query: 239 RKQLREGLSPKERRGLMHDRFILLKRHTALTEMD-KITLDLWTKNHPSLGTAYDLKESFF 297 RK R+ ++ KER+ LM DR++LL R L + D +I L +WT+N P LG AY+LKE FF Sbjct: 242 RKANRQNVTAKERKQLMRDRYVLLTRSKDLIDFDDQIKLQVWTQNFPLLGQAYELKEQFF 301 Query: 298 DIWECDSRQTAFLKYNEWKAKIPTELQSAFEPLTKAMKNWEEEIFAYFDHRITNAYMESL 357 DI+E +S A+ Y W + +P EL + FE L KAM NWEEEIF YF+ ITNAY ESL Sbjct: 302 DIYEVESINDAYKLYQNWLSNVPKELMTYFEDLIKAMNNWEEEIFNYFNTPITNAYTESL 361 Query: 358 NSLIRVINRLGRGYSFEALRAKILFTEGLKKQRKPE--------------ENFPMFNLPP 403 N LI+ +N +GRGYSFEALRAKILFT+G +K +K + + FP N Sbjct: 362 NRLIKTMNHVGRGYSFEALRAKILFTQGYRKVKKKKKFKEVEVTFGKMLPDQFP--NWSQ 419 Query: 404 IGIVREPEEIILPGIDFSTLIEKLEKG 430 G EEI G +FSTL + +E+G Sbjct: 420 TGYEWVYEEIY--GAEFSTLTKAMEEG 444 >ref|NP_689008.1| transposase, ISL3 family [Streptococcus agalactiae 2603V/R] gb|AAN00881.1| transposase, ISL3 family [Streptococcus agalactiae 2603V/R] Length = 417 Score = 428 bits (1101), Expect = e-118, Method: Compositional matrix adjust. Identities = 211/387 (54%), Positives = 274/387 (70%), Gaps = 9/387 (2%) Query: 16 ENEFDFLIQVVTSSPPLACPHCGCIANLYKHDSREQICMDLPIHGKRVGLLIKRQRYRCR 75 ENE D + +V PP CP CG LYKH SR Q+ MDLPI KRVGL + R+RY+CR Sbjct: 18 ENETDMMFKVEAVGPPERCPECG-FDKLYKHSSRNQLIMDLPIRLKRVGLHLNRRRYKCR 76 Query: 76 DCNRTFWEHLDHTIDEKRNCTKRLLSYIEKQSLQRTFVSISEDVGLHEKTIRNIFRDYIN 135 +C T ++DEKR+ TKRLL I++QS+ +TFV ++E VG+ EKTIRN+F+DY+ Sbjct: 77 ECGSTI------SVDEKRSMTKRLLKSIQEQSMSKTFVEVAESVGVDEKTIRNVFKDYVA 130 Query: 136 RLKETLRFETPNWLGIDEIHIIK-PRCVLTNIEERTLLDVLPNRNKETVVGYLSGLPNRG 194 + +FETP WLGIDEIHII+ PR VLTNIE RT+ D+ PNRNKETV+ LS + +R Sbjct: 131 LKEREYQFETPKWLGIDEIHIIRRPRLVLTNIERRTIYDIKPNRNKETVIQRLSEISDRT 190 Query: 195 EIQYVTMNMWQPYKDAVRAILPKALIIVDKFHVVRMANQALETIRKQLREGLSPKERRGL 254 I+YVTM+MW+PYKDAV ILP+A ++VDKFHVVRMANQAL+ +RK L+ +S KERR L Sbjct: 191 YIEYVTMDMWKPYKDAVNTILPQAKVVVDKFHVVRMANQALDNVRKSLKAHMSQKERRTL 250 Query: 255 MHDRFILLKRHTALTEMDKITLDLWTKNHPSLGTAYDLKESFFDIWECDSRQTAFLKYNE 314 M +RFILLKR L E + LD W N P+L AY+LKE F+ IW+ L+Y++ Sbjct: 251 MRERFILLKRKHDLNERESFLLDTWLGNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQ 310 Query: 315 WKAK-IPTELQSAFEPLTKAMKNWEEEIFAYFDHRITNAYMESLNSLIRVINRLGRGYSF 373 W+ + + + + A++ L +A+ NW EIF YFD R+TNAY ES+NS+IR + R+GRGYSF Sbjct: 311 WRHRCMSSNSKDAYKDLVRAVDNWHVEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSF 370 Query: 374 EALRAKILFTEGLKKQRKPEENFPMFN 400 +ALRAKILF E L K+RKP N FN Sbjct: 371 DALRAKILFNEKLHKKRKPRFNSSAFN 397 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.323 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 641,174,885 Number of extensions: 26492155 Number of successful extensions: 72643 Number of sequences better than 1.0e-05: 162 Number of HSP's gapped: 72419 Number of HSP's successfully gapped: 162 Length of query: 432 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 303 Effective length of database: 406,989,807 Effective search space: 123317911521 Effective search space used: 123317911521 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)