BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0352.0004.p (484 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_813034.1| exo-poly-alpha-D-galacturonosidase precursor [B... 464 e-129 ref|NP_813098.1| exo-poly-alpha-D-galacturonosidase precursor [B... 446 e-124 ref|NP_693009.1| hypothetical protein OB2088 [Oceanobacillus ihe... 268 2e-70 ref|ZP_00067822.1| hypothetical protein [Microbulbifer degradans... 228 2e-58 ref|NP_809931.1| polygalacturonase (Pectinase) [Bacteroides thet... 226 9e-58 >ref|NP_813034.1| exo-poly-alpha-D-galacturonosidase precursor [Bacteroides thetaiotaomicron VPI-5482] gb|AAO79228.1| exo-poly-alpha-D-galacturonosidase precursor [Bacteroides thetaiotaomicron VPI-5482] Length = 528 Score = 464 bits (1193), Expect = e-129, Method: Compositional matrix adjust. Identities = 226/497 (45%), Positives = 321/497 (64%), Gaps = 18/497 (3%) Query: 3 SNPET-----------IAYPVPEL--PRIPGRTFVITDYGAEEGGFTLCTSAIQAALDAC 49 SNPE+ + + +PE+ P P I D+GA G TL T AI A+ A Sbjct: 26 SNPESGTKEQGDVYRNLPFSMPEVSQPSFPDYEVNIRDFGALSDGVTLNTEAINNAIKAV 85 Query: 50 AAMGGGKVVIPAGIWRTGPLTLHSRINLSADQGALVQFDPDPALYPLLSSQYEGNAGWRC 109 ++ GGGKV+IP G+W TGP+ L S +NL A++ AL+ F D +LYP++ + +EG RC Sbjct: 86 SSKGGGKVIIPEGLWLTGPVVLLSNVNLYAEKNALIVFSSDTSLYPIIDTSFEGLDTKRC 145 Query: 110 QAPLDGEGLSDVAITGEGVFDGSGEGWRPVKRFKMTEKQWNHLIESGGVLDPQGEVWWPS 169 Q+P+ ++AITG GVFDG+G+ WRPVK+ KMTE+QW +L+ SGG +D G+VW+P Sbjct: 146 QSPISAMNAENIAITGNGVFDGAGDRWRPVKKDKMTERQWKNLVSSGGKVDENGKVWYPD 205 Query: 170 REAMEGEGFCRKLAD-KGDTRMEAFLPARAYLRPTLLSLRNCRRVLLDGPTFQNSPAWCL 228 A++ + + + E + +++LRP +LS+ +R+LL+G TF+NSP WC+ Sbjct: 206 AGALKASVLMTGQNNGQKEITDEEWTYMKSWLRPVMLSIVKSKRILLEGVTFKNSPGWCI 265 Query: 229 HPFGCEQITVRNVHVRNPWYSQNGDGLDLESCTHALVEHSSFDVGDDAICLKSGKDEEGR 288 HP CE +T+ +V V NPWYSQNGD LD+ESC + LV + FD GDDAICLKSGKDE+GR Sbjct: 266 HPLSCESLTLNDVKVFNPWYSQNGDALDVESCKNVLVTNCFFDAGDDAICLKSGKDEDGR 325 Query: 289 KRGLPCRYITIRNCTVYHGHGGVVIGSEMSGGVHAVRVSDCLFIGTDIGLRFKSTRGRGG 348 +RG PC + I+N TV HGHGG VIGSEMSGGV V VS C F+GTD+GLRFKSTRGRGG Sbjct: 326 RRGEPCENVIIKNNTVLHGHGGFVIGSEMSGGVRNVYVSGCSFVGTDVGLRFKSTRGRGG 385 Query: 349 VVEDIVMENISMTGIIHEAVSFHMFYAGVEGSEGYDEQYFSVTEETPQFRKITLKNLVCH 408 VVE+I ++NI+M I ++A++ ++YA + E V EETP FR I + N++C Sbjct: 386 VVENIFIDNINMIDISNDALTMDLYYAVNDFPE---TPIPDVNEETPVFRNIYISNVLCR 442 Query: 409 GAATALLVNGLPEMPLADLTIEQFRAV-SERGIVLRHATGLTLDRIWLQTAETPEITAHR 467 GA A+ NGLPEMPL ++ I+ +++GIV+ A+ + ++ I ++ E P I Sbjct: 443 GAERAVYFNGLPEMPLKNIFIKNMTVTNAKKGIVINQASQVNMENIKVEDPEAPGIQIKN 502 Query: 468 CSQVVLTQSEDLTITGK 484 + +++ E +GK Sbjct: 503 ATGIIINGKEYKKDSGK 519 >ref|NP_813098.1| exo-poly-alpha-D-galacturonosidase precursor [Bacteroides thetaiotaomicron VPI-5482] gb|AAO79292.1| exo-poly-alpha-D-galacturonosidase precursor [Bacteroides thetaiotaomicron VPI-5482] Length = 538 Score = 446 bits (1147), Expect = e-124, Method: Compositional matrix adjust. Identities = 220/477 (46%), Positives = 307/477 (64%), Gaps = 10/477 (2%) Query: 11 PVPELPRIPGRTFVITDYGAEEGGFTLCTSAIQAALDACAAMGGGKVVIPAGIWRTGPLT 70 P + P P I+ +GA+ G TL T AI A+ GGGKV+IP G W TGP+ Sbjct: 47 PKVQQPVFPAYEVNISKFGAKGDGMTLNTKAINDAIKEVNQRGGGKVIIPEGTWLTGPIE 106 Query: 71 LHSRINLSADQGALVQFDPDPALYPLLSSQYEGNAGWRCQAPLDGEGLSDVAITGEGVFD 130 L S +NL ++ AL+ F D YP++ + +EG RCQ+P+ ++AITG G+FD Sbjct: 107 LLSNVNLYTERNALILFTGDFEAYPIIPTSFEGLETRRCQSPISARNAENIAITGYGIFD 166 Query: 131 GSGEGWRPVKRFKMTEKQWNHLIESGGVLDPQGEVWWPSREAMEGEGFCRKL-ADKGDTR 189 G+G+ WRPVK+ K+T QWN L++SGGVLD Q +W+P+ +++G C+ +G Sbjct: 167 GNGDCWRPVKKEKLTASQWNKLVKSGGVLDEQERIWYPTAGSLKGAMACKDFNVPEGINT 226 Query: 190 MEAFLPARAYLRPTLLSLRNCRRVLLDGPTFQNSPAWCLHPFGCEQITVRNVHVRNPWYS 249 E + RA+LRP LL+ +R+LL+G TF+NSP+WCLHP CE TV N+ V NPWYS Sbjct: 227 DEEWNEIRAWLRPVLLNFVKSKRILLEGVTFKNSPSWCLHPLSCEDFTVNNIQVINPWYS 286 Query: 250 QNGDGLDLESCTHALVEHSSFDVGDDAICLKSGKDEEGRKRGLPCRYITIRNCTVYHGHG 309 QNGD LDLESC +AL+ +S FD GDDAIC+KSGKDE GR+RG PC+ + ++N TV HGHG Sbjct: 287 QNGDALDLESCKNALILNSVFDAGDDAICIKSGKDENGRRRGEPCQNVIVKNNTVLHGHG 346 Query: 310 GVVIGSEMSGGVHAVRVSDCLFIGTDIGLRFKSTRGRGGVVEDIVMENISMTGIIHEAVS 369 G V+GSEMSGGV + V DC F+GTD+GLRFKSTRGRGGVVE+I + NI+M I +E + Sbjct: 347 GFVVGSEMSGGVKNIYVEDCTFLGTDVGLRFKSTRGRGGVVENIYINNINMINIPNEPLL 406 Query: 370 FHMFYAGVEGSEGYDEQYFS--------VTEETPQFRKITLKNLVCHGAATALLVNGLPE 421 F +FY G E +E S VTEETP FR I + N++C G+ A+ NGLPE Sbjct: 407 FDLFYGGKGAGEESEEDLLSRMKTAIPPVTEETPAFRDIHISNVICRGSGRAMFFNGLPE 466 Query: 422 MPLADLTIEQFRAV-SERGIVLRHATGLTLDRIWLQTAETPEITAHRCSQVVLTQSE 477 MP+ ++T++ + G+V+ G+TL+ I++++ + I + ++ + E Sbjct: 467 MPIRNVTVKNVVMTEATDGVVISQVDGVTLENIYVESTKGKNILNVKSAKNLKVDGE 523 >ref|NP_693009.1| hypothetical protein OB2088 [Oceanobacillus iheyensis HTE831] dbj|BAC14044.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 495 Score = 268 bits (686), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 153/464 (32%), Positives = 248/464 (53%), Gaps = 46/464 (9%) Query: 25 ITDYGAEEGGFTLCTSAIQAALDACAAMGGGKVVIPAGIWRTGPLTLHSRINLSADQGAL 84 IT++GA+ T T +I +A+ CA GGG + IPAG + TGP++L S + + + GA Sbjct: 5 ITEFGAKGDSNTDNTESISSAIKRCADSGGGTLYIPAGTYLTGPISLISNLTIYIESGAK 64 Query: 85 VQFDPDPALYPLLSSQYEGNAGWRCQAPLDGEGLSDVAITGEGVFDGSGEGWRPVKRFKM 144 + F D + YP + +++ G + L G GL++V+I G G DG GE W V Sbjct: 65 LVFIDDFSAYPPVKTRWSGYECYGFSPLLYGNGLTNVSIIGSGCIDGQGEAWWEV----- 119 Query: 145 TEKQWNHLIESGGVLD-PQGEVWWPSREAMEGEGFCRKLADKGDTRMEAFLPARAYLRPT 203 NHL+ G D PQ ++E E + + + +T + + P++ +LRP Sbjct: 120 -----NHLLRKGEEYDHPQ------TKEIAE---LNKSITEPKNTNLVEW-PSQ-FLRPP 163 Query: 204 LLSLRNCRRVLLDGPTFQNSPAWCLHPFGCEQITVRNVHVRNPWYSQNGDGLDLESCTHA 263 LL + +C V LDG T +NSP W H C+ +T+ NV +NPW + NGDGLDL+SC++ Sbjct: 164 LLQMYDCDSVTLDGITLENSPFWNTHFVYCDNVTIHNVKFKNPWDTPNGDGLDLDSCSNV 223 Query: 264 LVEHSSFDVGDDAICLKSGKDEEGRKRGLPCRYITIRNCTVYHGHGGVVIGSEMSGGVHA 323 V + FDVGDD + KSG +E+GR+ P + + NCT+ +GHGG+V+GSE SGG+ Sbjct: 224 RVSNCHFDVGDDCLAFKSGINEDGRRVARPTENVAVTNCTMKNGHGGIVMGSENSGGIRN 283 Query: 324 VRVSDCLFIGTDIGLRFKSTRGRGGVVEDIVMENISMTGIIHEAVSFHMFYAGVEGSEGY 383 + VS+C+FIGTD G+R K+ R RG + DI+++NI M G++ + G++ S+ Sbjct: 284 IAVSNCVFIGTDRGIRLKTNRARGSYIRDILIDNIYMDGVLCPLAINSFYRHGLDKSDSL 343 Query: 384 DEQY--FSVTEETPQFRKITLKNLVCHGAATAL-LVNGLPEMPLADLTIEQFRA------ 434 + ++E+TP+ I + N+ +A + GLPE + D+ + Sbjct: 344 INELSPIEISEKTPEIEYIHISNVTARNCRSAAGFIYGLPEKYVKDIILRHVLIEMTINP 403 Query: 435 ---------------VSERGIVLRHATGLTLDRIWLQTAETPEI 463 ++ GI ++ G+ R+ ++T + P + Sbjct: 404 DEQGGEPDMVKEPIFMAGEGIYCKYVDGIEFHRVRVETRQGPAL 447 >ref|ZP_00067822.1| hypothetical protein [Microbulbifer degradans 2-40] Length = 463 Score = 228 bits (582), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 145/456 (31%), Positives = 227/456 (49%), Gaps = 57/456 (12%) Query: 15 LPRIPGRTFVITDYGAEEGGFTLCTSAIQAALDACAAMGGGKVVIPAGIWRTGPLTLHSR 74 +P P + F + ++G +EG T A + A+ AC+ GGGKVV+PAG + TG + L S Sbjct: 45 IPTFPNKEFNLLEFGGKEGSDN--TLAFKKAIAACSKAGGGKVVVPAGRFETGAIHLESN 102 Query: 75 INLSADQGALVQFDPDPALY-PLLSSQYEGNAGWRCQAPLDGEGLSDVAITGEGVFDGSG 133 +NL +GA + F DP Y P + +++EG + G +++AITG+G DG Sbjct: 103 VNLHISEGATIAFFTDPKYYLPAVFTRWEGMECMGYSPLIYAYGKTNIAITGKGTLDGQA 162 Query: 134 E-----GWRPVKRFKMTEKQWNHLIESGGVLDPQGEVWWPSREAMEGEGFCRKLADKGDT 188 + W+ K+W G +PS++ + F + A+ GD Sbjct: 163 DPTHWWAWK-------GNKEW-------------GVEGYPSQKESRNQLFAQ--AEAGDP 200 Query: 189 RMEAFLPARAYLRPTLLSLRNCRRVLLDGPTFQNSPAWCLHPFGCEQITVRNVHVRNPWY 248 E YLRP+ + C VL++ T N+P W LHP + +TVR VH+ + Sbjct: 201 VRERVYADGHYLRPSFVQPYKCENVLIEDITIINAPFWLLHPTLSQNVTVRGVHLES--L 258 Query: 249 SQNGDGLDLESCTHALVEHSSFDVGDDAICLKSGKDEEGRKRGLPCRYITIRNCTVYHGH 308 N DG D ESC + ++E+ F+ GDD I +KSG++ +GR+ P + IRNC + GH Sbjct: 259 GPNSDGCDPESCKNVVIENCFFNTGDDCIAIKSGRNNDGRRLATPTENVIIRNCKMEAGH 318 Query: 309 GGVVIGSEMSGGVHAVRVSDCLFIGTDI--GLRFKSTRGRGGVVEDIVMENISMTGIIHE 366 GGVVIGSE+SGGV V + + D+ G+R K+ RGG++E+I + N ++ G + + Sbjct: 319 GGVVIGSEISGGVRNVFAENNVMSSPDLEKGIRIKTNSVRGGLLENIYVRNCTI-GEVQQ 377 Query: 367 AVSFHMFYAGVEGSEGYDEQYFSVTEETPQFRKITLKNLVCHGAATALLVNGLPEMPLAD 426 A+ + Y EG G + P R + ++NLVC A + G P Sbjct: 378 AIVINFQYE--EGDAGKFD---------PTVRNVEIRNLVCQHALQVFNIRGFERAP--- 423 Query: 427 LTIEQFRAVSERGI------VLRHATGLTLDRIWLQ 456 I+ FR + + V+ H TGL +D + + Sbjct: 424 --IQNFRIIDSTFVRGDNPGVIEHTTGLVIDNVQVN 457 >ref|NP_809931.1| polygalacturonase (Pectinase) [Bacteroides thetaiotaomicron VPI-5482] gb|AAO76125.1| polygalacturonase (Pectinase) [Bacteroides thetaiotaomicron VPI-5482] Length = 445 Score = 226 bits (576), Expect = 9e-58, Method: Compositional matrix adjust. Identities = 140/434 (32%), Positives = 213/434 (49%), Gaps = 25/434 (5%) Query: 29 GAEEGGFTLCTSAIQAALDACAAMGGGKVVIPAGIWRTGPLTLHSRINLSADQGALVQFD 88 GA+ G L T I + +D GGG + PAG + TG + L S I L + GA + F Sbjct: 30 GAKANGKALNTKLINSTIDRLNRGGGGTLFFPAGTYLTGSIHLKSNITLELEAGATLLFS 89 Query: 89 PDPALY-PLLSSQYEGNAGWRCQAPLDGEGLSDVAITGEGVFDGSGEG-WRPVKRFKMTE 146 + Y P + ++EG Q + ++ I GEG DG G+ W R + Sbjct: 90 DNFDDYLPFVEVRHEGVMMKSFQPLIYAVDAENITIKGEGTLDGQGKKWWMEFFRVMID- 148 Query: 147 KQWNHLIESGGVLDPQGEVWWPSREAMEGEGFCRKLADKGDTRMEAFLPARAYLRPTLLS 206 ++ G+ D + P +A +K R + RP + Sbjct: 149 ------LKDNGMRDINK--YQPMWDAQNDTTAIYAETNKD----YVSTLQRRFFRPPFIQ 196 Query: 207 LRNCRRVLLDGPTFQNSPAWCLHPFGCEQITVRNVHVRNPWYSQNGDGLDLESCTHALVE 266 C++V ++G NSP W ++P C+ +T++ + + N S N DG++ ESC + + Sbjct: 197 PVRCKKVKIEGVKIVNSPFWTVNPEFCDNVTIKGITINN-VPSPNTDGINPESCRNVHIS 255 Query: 267 HSSFDVGDDAICLKSGKDEEGRKRGLPCRYITIRNCTVYHGHGGVVIGSEMSGGVHAVRV 326 VGDD I +KSG+D + R+ G+PC ITI NCT+ GHGGVVIGSEMSG V V + Sbjct: 256 DCHISVGDDCITIKSGRDAQARRLGVPCENITITNCTMLSGHGGVVIGSEMSGSVRKVTI 315 Query: 327 SDCLFIGTDIGLRFKSTRGRGGVVEDIVMENISMTGIIHEAVSFHMFYAGVEGSEGYDEQ 386 S+C+F GTD G+R KSTRGRGGVVEDI + NI M+ I EAV ++ Y+ + Sbjct: 316 SNCVFDGTDRGIRIKSTRGRGGVVEDIRVSNIVMSNIKQEAVVLNLKYSQMPAE------ 369 Query: 387 YFSVTEETPQFRKITLKNLVCHGAATALLVNGLPEMPLADLTIEQFRAVSER-GIVLRHA 445 + +E TP FR + + + T + + GL E P++D+ + R + Sbjct: 370 --AKSERTPIFRNVHISGMTVTDVKTPIKIVGLEEAPISDIVLRDIHIQGARQKCIFEDC 427 Query: 446 TGLTLDRIWLQTAE 459 +T+D + + E Sbjct: 428 ERITMDDVIINGEE 441 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 772,150,559 Number of extensions: 34826133 Number of successful extensions: 62532 Number of sequences better than 1.0e-05: 215 Number of HSP's gapped: 62308 Number of HSP's successfully gapped: 248 Length of query: 484 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 354 Effective length of database: 404,274,708 Effective search space: 143113246632 Effective search space used: 143113246632 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)