BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0060.p (331 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_389710.1| alternate gene name: yotA~similar to hypothetic... 359 4e-98 pir||T44810 conserved hypothetical protein yngK [imported] - Bac... 356 3e-97 ref|NP_656316.1| DUF187, Uncharacterized BCR, COG1649 [Bacillus ... 353 4e-96 gb|AAF32334.1| FenI [Bacillus subtilis] 349 4e-95 ref|NP_832154.1| FenI [Bacillus cereus ATCC 14579] >gi|29896074|... 347 2e-94 >ref|NP_389710.1| alternate gene name: yotA~similar to hypothetical proteins [Bacillus subtilis] pir||H69893 conserved hypothetical protein yngK - Bacillus subtilis emb|CAA74215.1| yngK [Bacillus subtilis] emb|CAB13711.1| yngK [Bacillus subtilis subsp. subtilis str. 168] Length = 510 Score = 359 bits (922), Expect = 4e-98, Method: Compositional matrix adjust. Identities = 168/331 (50%), Positives = 217/331 (65%), Gaps = 6/331 (1%) Query: 1 LLDKLQKTGFNAVFVQVRPSGDSLYPSVLVPWSKVLTGSQGKNPGYDPLEFMISAAHSRG 60 LLD +QK G NAV VQ++P+ D+ YPS PWS+ LTG QGK+PGYDPL FMI H R Sbjct: 67 LLDDVQKMGMNAVIVQIKPTADAFYPSAYGPWSEYLTGVQGKDPGYDPLAFMIEETHKRN 126 Query: 61 MQFHAWFNPFRATTDANTAN-LAGNHVAKAHPEWIVKADSKLYINPGIPEARQHIIDTVM 119 ++FHAWFNP+R T + N L+ +H A+ HP+W+ ++LY +PGIPEAR I+ + Sbjct: 127 LEFHAWFNPYRITMNHTDLNKLSEDHPARKHPDWVAAYGNQLYYHPGIPEARDFIVKGIE 186 Query: 120 EVVKGYDIDGVHLDDYFYP----SGSFADDEAFKTYNSKSISSKGDWRRDNINDFISRLG 175 EVVK YDID VH+DDYFYP F D ++ Y + S+ DWRRDN+N + ++ Sbjct: 187 EVVKHYDIDAVHMDDYFYPYKIAGQEFPDQAQYEQYGKDAFSNIDDWRRDNVNQLVKQIN 246 Query: 176 QSIHGVKSDVSFGVSPFGVWRNKKADSTGSDTTAGVSAYDDMYADTRTWIRSGWIDYIAP 235 Q+I K V FG+SPFGVWRN D TGS+T AGV YDD+YADTR WI+ G IDYIAP Sbjct: 247 QTIKAAKPYVKFGISPFGVWRNAADDPTGSNTKAGVRNYDDLYADTRHWIQEGDIDYIAP 306 Query: 236 QIYWSLSFNAARYDKLVDWWVNEVKGSGVKLYIGQATYKVGTDKSAEWQSSEQIINQLKY 295 QIYWS+ FNAA YD L DWW NEVK V LYIGQA YK+ + W E+ + Q+ Sbjct: 307 QIYWSIGFNAAAYDVLADWWSNEVKNRPVHLYIGQAAYKINNNFDPPWSDPEEYVRQITL 366 Query: 296 NEKYNEVAGSIMFRANDIVSRDPFGLNSLLT 326 N + V GS+ F D+ +++P G+ L+ Sbjct: 367 NRQLELVKGSMHFSLKDL-NKNPLGIKDSLS 396 >pir||T44810 conserved hypothetical protein yngK [imported] - Bacillus subtilis gb|AAF08799.1| YngK [Bacillus subtilis] Length = 510 Score = 356 bits (914), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 168/330 (50%), Positives = 216/330 (65%), Gaps = 6/330 (1%) Query: 1 LLDKLQKTGFNAVFVQVRPSGDSLYPSVLVPWSKVLTGSQGKNPGYDPLEFMISAAHSRG 60 LLD +QK G NAV VQ++P+ D+ YPS PWS+ LTG QGK+PGYDPL FMI H R Sbjct: 67 LLDDVQKMGMNAVIVQIKPTADAFYPSAYGPWSEYLTGVQGKDPGYDPLAFMIEETHKRN 126 Query: 61 MQFHAWFNPFRATTDANTAN-LAGNHVAKAHPEWIVKADSKLYINPGIPEARQHIIDTVM 119 ++FHAWFNP+R T + N L+ +H A+ HP+W+ ++LY +PGI EAR I+ + Sbjct: 127 LEFHAWFNPYRITMNHTDLNKLSKDHPARQHPDWVAAYGNQLYYHPGISEARDFIVKGIE 186 Query: 120 EVVKGYDIDGVHLDDYFYP----SGSFADDEAFKTYNSKSISSKGDWRRDNINDFISRLG 175 EVVK YDID VH+DDYFYP F D ++ Y + S+ DWRRDN+N + ++ Sbjct: 187 EVVKHYDIDAVHMDDYFYPYKIAGQEFPDHAQYEQYGKGTFSNIDDWRRDNVNQLVKQIN 246 Query: 176 QSIHGVKSDVSFGVSPFGVWRNKKADSTGSDTTAGVSAYDDMYADTRTWIRSGWIDYIAP 235 Q+I K V FG+SPFGVWRN D TGS+T AGV YDD+YADTR WI+ G IDYIAP Sbjct: 247 QTIKTAKPYVKFGISPFGVWRNAADDPTGSNTKAGVRNYDDLYADTRHWIQEGDIDYIAP 306 Query: 236 QIYWSLSFNAARYDKLVDWWVNEVKGSGVKLYIGQATYKVGTDKSAEWQSSEQIINQLKY 295 QIYWS+ FNAA YD L DWW NEVK V LYIGQA YK+ + W E+ + Q+ Sbjct: 307 QIYWSIGFNAAAYDVLADWWSNEVKNRPVHLYIGQAAYKINNNFDPPWSDPEEYVRQITL 366 Query: 296 NEKYNEVAGSIMFRANDIVSRDPFGLNSLL 325 N + V GS+ F D+ +++PFG+ L Sbjct: 367 NRQLELVKGSMHFSLKDL-NKNPFGIKDRL 395 >ref|NP_656316.1| DUF187, Uncharacterized BCR, COG1649 [Bacillus anthracis A2012] Length = 510 Score = 353 bits (905), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 163/331 (49%), Positives = 219/331 (66%), Gaps = 6/331 (1%) Query: 1 LLDKLQKTGFNAVFVQVRPSGDSLYPSVLVPWSKVLTGSQGKNPGYDPLEFMISAAHSRG 60 LLD ++ TG NAV VQ++P+ D+ YPS PWS+ +TG+QGK+PGYDPL FM+ AH R Sbjct: 62 LLDDVKNTGMNAVVVQIKPTADAFYPSNYGPWSEYITGTQGKDPGYDPLAFMVDEAHKRN 121 Query: 61 MQFHAWFNPFRATTDANTAN-LAGNHVAKAHPEWIVKADSKLYINPGIPEARQHIIDTVM 119 ++FHAW NP+R T + N L+ NH AK HP+WIV KLY NPGIPE ++ I + + Sbjct: 122 IEFHAWINPYRITMNHTDINRLSNNHPAKQHPDWIVPYGGKLYYNPGIPEVKKFITEGAL 181 Query: 120 EVVKGYDIDGVHLDDYFYP----SGSFADDEAFKTYNSKSISSKGDWRRDNINDFISRLG 175 E+V+ YDID +H+DDYFYP F D + ++TYN+ ++ DWRR+N+N+ + L Sbjct: 182 EIVQNYDIDALHMDDYFYPYKVAGEQFPDQKTYETYNNGRFTNIEDWRRNNVNELVKDLN 241 Query: 176 QSIHGVKSDVSFGVSPFGVWRNKKADSTGSDTTAGVSAYDDMYADTRTWIRSGWIDYIAP 235 +I KS V FG+SPFGVWRN D TGS+TTAG YDD+YADTR WI+ G+IDYI P Sbjct: 242 TAIKHEKSYVKFGISPFGVWRNIADDPTGSNTTAGQRNYDDLYADTREWIQKGFIDYITP 301 Query: 236 QIYWSLSFNAARYDKLVDWWVNEVKGSGVKLYIGQATYKVGTDKSAEWQSSEQIINQLKY 295 QIYW++ F A YD LVDWWV E + LYIGQA YK+ + W + E+ Q++ Sbjct: 302 QIYWNIGFTPAAYDILVDWWVKETNNKPIHLYIGQAAYKINNNSVPAWSNPEEYPRQIEL 361 Query: 296 NEKYNEVAGSIMFRANDIVSRDPFGLNSLLT 326 N Y E+ GS+ F DI + +P G+ L+ Sbjct: 362 NRLYPEIKGSMHFSLKDI-NNNPLGIKDRLS 391 >gb|AAF32334.1| FenI [Bacillus subtilis] Length = 510 Score = 349 bits (896), Expect = 4e-95, Method: Compositional matrix adjust. Identities = 166/330 (50%), Positives = 213/330 (64%), Gaps = 6/330 (1%) Query: 1 LLDKLQKTGFNAVFVQVRPSGDSLYPSVLVPWSKVLTGSQGKNPGYDPLEFMISAAHSRG 60 LLD +Q NAV VQ++P+ D+ YPS PWS+ LTG QGK+PGYDPL FMI AH R Sbjct: 67 LLDDVQSMRMNAVIVQIKPTADAFYPSAYGPWSEYLTGVQGKDPGYDPLAFMIEEAHKRN 126 Query: 61 MQFHAWFNPFRATTDANTAN-LAGNHVAKAHPEWIVKADSKLYINPGIPEARQHIIDTVM 119 ++FHAW NP+R T + N L+ +H A+ HP+W+V +LY NPGIPEAR I++ + Sbjct: 127 LEFHAWVNPYRITMNHTDLNKLSDDHPARKHPDWVVAYGKQLYYNPGIPEARDFIVEGIE 186 Query: 120 EVVKGYDIDGVHLDDYFYP----SGSFADDEAFKTYNSKSISSKGDWRRDNINDFISRLG 175 EVVK YD+D VH+DDYFYP F D ++ Y + DWRRDN+N +S++ Sbjct: 187 EVVKHYDVDAVHMDDYFYPYKIAGQEFPDTVQYEQYGKYTFPDIDDWRRDNVNQLVSQIN 246 Query: 176 QSIHGVKSDVSFGVSPFGVWRNKKADSTGSDTTAGVSAYDDMYADTRTWIRSGWIDYIAP 235 Q+I K V FG+SPFGVWRN + D +GS+T AGV YDD+YADTR WI+ G IDYIAP Sbjct: 247 QTIKAEKPYVKFGISPFGVWRNAEDDPSGSNTKAGVRNYDDLYADTRHWIQQGDIDYIAP 306 Query: 236 QIYWSLSFNAARYDKLVDWWVNEVKGSGVKLYIGQATYKVGTDKSAEWQSSEQIINQLKY 295 QIYWS+ FNAA YD L DWW NEVK V LYIGQA YK+ + W E Q+ Sbjct: 307 QIYWSIGFNAAAYDVLADWWSNEVKNRPVHLYIGQAAYKINNNFDPPWSDPEGYGRQINL 366 Query: 296 NEKYNEVAGSIMFRANDIVSRDPFGLNSLL 325 N + V GS+ F D+ +++P G+ L Sbjct: 367 NRQLELVKGSMHFSLKDL-NKNPLGIKDRL 395 >ref|NP_832154.1| FenI [Bacillus cereus ATCC 14579] gb|AAP09355.1| FenI [Bacillus cereus ATCC 14579] Length = 519 Score = 347 bits (891), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 160/331 (48%), Positives = 216/331 (65%), Gaps = 6/331 (1%) Query: 1 LLDKLQKTGFNAVFVQVRPSGDSLYPSVLVPWSKVLTGSQGKNPGYDPLEFMISAAHSRG 60 LLD ++ TG NAV VQ++P+ D+ YPS PWS+ +TG+QGK+PGYDPL FMI H R Sbjct: 71 LLDDVKSTGMNAVVVQIKPTADAFYPSNYGPWSEYITGTQGKDPGYDPLAFMIEETHKRN 130 Query: 61 MQFHAWFNPFRATTDANTAN-LAGNHVAKAHPEWIVKADSKLYINPGIPEARQHIIDTVM 119 ++FHAW NP+R T + N L+ NH A+ HP+W+V KLY NPGIPE ++ I + + Sbjct: 131 IEFHAWINPYRITMNHTDINRLSNNHPARQHPDWVVTYGGKLYYNPGIPEVKKFITEGAL 190 Query: 120 EVVKGYDIDGVHLDDYFYP----SGSFADDEAFKTYNSKSISSKGDWRRDNINDFISRLG 175 E+V+ YDID +H+DDYFYP F D + ++TYN+ ++ DWRR+N+N+ + L Sbjct: 191 EIVENYDIDALHMDDYFYPYKVAGEEFPDQKTYETYNNGRFTNIEDWRRNNVNELVKDLN 250 Query: 176 QSIHGVKSDVSFGVSPFGVWRNKKADSTGSDTTAGVSAYDDMYADTRTWIRSGWIDYIAP 235 +I KS V FG+SPFGVWRN D TGS+TTAG YDD+YADTR WI+ G+IDYI P Sbjct: 251 TAIKQEKSYVKFGISPFGVWRNIADDPTGSNTTAGQRNYDDLYADTREWIQKGYIDYITP 310 Query: 236 QIYWSLSFNAARYDKLVDWWVNEVKGSGVKLYIGQATYKVGTDKSAEWQSSEQIINQLKY 295 QIYW++ F A YD LVDWWV E + LYIGQA YK+ + W E+ Q+ Sbjct: 311 QIYWNIGFTPAAYDILVDWWVKETNNKPLHLYIGQAAYKINNNSVPAWSDPEEYPKQIAL 370 Query: 296 NEKYNEVAGSIMFRANDIVSRDPFGLNSLLT 326 N Y ++ GS+ F DI + +P G+ L+ Sbjct: 371 NRLYPDIKGSMHFSLKDI-NNNPLGVKDRLS 400 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 530,901,823 Number of extensions: 22679897 Number of successful extensions: 51935 Number of sequences better than 1.0e-05: 92 Number of HSP's gapped: 51748 Number of HSP's successfully gapped: 92 Length of query: 331 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 205 Effective length of database: 415,135,104 Effective search space: 85102696320 Effective search space used: 85102696320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)