BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0022.p (189 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_349292.1| Trehalose/maltose hydrolase (phosphorylase) [Cl... 110 1e-23 ref|NP_785303.1| maltose phosphorylase [Lactobacillus plantarum ... 108 6e-23 pdb|1H54|A Chain A, Maltose Phosphorylase From Lactobacillus Bre... 105 4e-22 ref|NP_784013.1| maltose phosphorylase [Lactobacillus plantarum ... 96 4e-19 ref|ZP_00047083.1| COG1554: Trehalose and maltose hydrolases (po... 96 6e-19 >ref|NP_349292.1| Trehalose/maltose hydrolase (phosphorylase) [Clostridium acetobutylicum] pir||E97230 trehalose/maltose hydrolase (phosphorylase) [imported] - Clostridium acetobutylicum gb|AAK80632.1| Trehalose/maltose hydrolase (phosphorylase) [Clostridium acetobutylicum] Length = 757 Score = 110 bits (275), Expect = 1e-23, Method: Composition-based stats. Identities = 56/158 (35%), Positives = 89/158 (56%), Gaps = 2/158 (1%) Query: 21 KYLKVDPWAVIEEGFDPLRNRTSESIFSVGNEYMGVRGYAEEGYSGDSLPGSYFNGLH-- 78 KY KVD W ++E+ F +N+ SESIFS+GN YMG RG EE YSG +L G+Y G++ Sbjct: 3 KYFKVDAWNIVEDNFMEEKNKISESIFSIGNGYMGQRGNFEEDYSGLTLKGTYMAGIYYP 62 Query: 79 EQLEIGNHYKGMIRSLRYMVNAVDWLHTRLTVEGEQLDLAHSRISDYSRRLDFRTGTYSR 138 ++ +G G + ++N+ +W+ ++ V G+ D++ +I + R L+ R G R Sbjct: 63 DRTRVGWWKNGYPKYFAKVLNSTNWIGIKVNVNGKCFDISRCKIESFKRVLNMREGYLYR 122 Query: 139 QFIWLLGSGKKLKLTFTRLVSMTDSHLGLQQVVFEPLN 176 Q I G+++KL R VS+ +G + PLN Sbjct: 123 QVIATFKDGERIKLETKRFVSIVSKEIGAIKYSITPLN 160 >ref|NP_785303.1| maltose phosphorylase [Lactobacillus plantarum WCFS1] emb|CAD64151.1| maltose phosphorylase [Lactobacillus plantarum WCFS1] Length = 748 Score = 108 bits (270), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 56/158 (35%), Positives = 92/158 (58%), Gaps = 3/158 (1%) Query: 21 KYLKVDPWAVIEEGFDPLRNRTSESIFSVGNEYMGVRGYAEEGYSGDSLPGSYFNGL--H 78 + V+PW V+ F P R ES+ S+GN YMG+RG+ EE Y+GD+LPG Y G+ Sbjct: 3 RIFDVNPWHVLTTDFKPENKRLQESMTSLGNGYMGMRGFFEEDYTGDTLPGIYLGGVWYP 62 Query: 79 EQLEIGNHYKGMIRSLRYMVNAVDWLHTRLTVEGEQLDLAHSRISDYSRRLDFRTGTYSR 138 ++ +G G ++NAV+++ + G ++DLA + SD+ LD + GT +R Sbjct: 63 DKTRVGWWKNGYPEYFGKVINAVNFIKINFKLNGTKIDLATANFSDFKLDLDMQHGTLTR 122 Query: 139 QFIWLLGSGKKLKLTFTRLVSMTDSHLGLQQVVFEPLN 176 FI + G+++++TF R +S+ L +Q+V FE L+ Sbjct: 123 SFI-VDQDGQRVRVTFERFLSVAQKELSVQKVTFENLS 159 >pdb|1H54|A Chain A, Maltose Phosphorylase From Lactobacillus Brevis pdb|1H54|B Chain B, Maltose Phosphorylase From Lactobacillus Brevis Length = 754 Score = 105 bits (263), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 59/158 (37%), Positives = 90/158 (56%), Gaps = 3/158 (1%) Query: 21 KYLKVDPWAVIEEGFDPLRNRTSESIFSVGNEYMGVRGYAEEGYSGDSLPGSYFNGL--H 78 + +V PW VI FDP R ES+ S+GN YMG+RG EEGYSGDSL G Y G+ Sbjct: 3 RIFEVQPWNVITHTFDPKDKRLQESMTSLGNGYMGMRGDFEEGYSGDSLQGIYLGGVWYP 62 Query: 79 EQLEIGNHYKGMIRSLRYMVNAVDWLHTRLTVEGEQLDLAHSRISDYSRRLDFRTGTYSR 138 ++ +G G + +VNAV+++ + + GE +DLA +ISD++ LD G +R Sbjct: 63 DKTRVGWWKNGYPKYFGKVVNAVNFIKLPIEINGEPVDLAKDKISDFTLDLDMHQGVLNR 122 Query: 139 QFIWLLGSGKKLKLTFTRLVSMTDSHLGLQQVVFEPLN 176 F+ G+ ++ L F R +S+ L +Q+V + L+ Sbjct: 123 SFVVERGA-VRVALNFQRFLSVAQPELSVQKVTVKNLS 159 >ref|NP_784013.1| maltose phosphorylase [Lactobacillus plantarum WCFS1] emb|CAD62851.1| maltose phosphorylase [Lactobacillus plantarum WCFS1] Length = 756 Score = 95.9 bits (237), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 55/162 (33%), Positives = 86/162 (53%), Gaps = 8/162 (4%) Query: 21 KYLKVDPWAVIEEGFDPLRNRTSESIFSVGNEYMGVRGYAEEGYSGDSLPGSYFNGL--H 78 + +VDPW VI P R ES+ S+GN YMG+RG EE YS D+L G Y G+ Sbjct: 3 RIFEVDPWHVISHELVPTDKRLQESMTSIGNGYMGMRGMFEEHYSNDTLKGIYIGGVWYP 62 Query: 79 EQLEIGNHYKGMIRSLRYMVNAVDWLHTRLTVEGEQLDLAHSRISDYSRRLDFRTGTYSR 138 ++ +G G ++N+V+++ +T++G+Q+DLA +SD++ LD TG R Sbjct: 63 DKTRVGWWKNGYPDYFGKVINSVNFIKVNITIDGDQVDLAKDTVSDFTLDLDMHTGILRR 122 Query: 139 QFIWLLGSGKKLKLTFTRLVSMT-----DSHLGLQQVVFEPL 175 FI G K+++ R VS+ D H + + EP+ Sbjct: 123 SFIITKGE-KRVQFMIDRFVSVAQKELFDVHYSVHNLGAEPV 163 >ref|ZP_00047083.1| COG1554: Trehalose and maltose hydrolases (possible phosphorylases) [Lactobacillus gasseri] Length = 758 Score = 95.5 bits (236), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 56/152 (36%), Positives = 80/152 (52%), Gaps = 3/152 (1%) Query: 21 KYLKVDPWAVIEEGFDPLRNRTSESIFSVGNEYMGVRGYAEEGYSGDSLPGSYFNGL--H 78 + +VDPW V F R ESI ++GN+YMG+RG EEGYSGD+L G+Y G+ Sbjct: 5 RIFEVDPWKVTTHEFSKEDKRLQESITAIGNDYMGMRGNFEEGYSGDTLQGTYLAGVWFP 64 Query: 79 EQLEIGNHYKGMIRSLRYMVNAVDWLHTRLTVEGEQLDLAHSRISDYSRRLDFRTGTYSR 138 ++ +G G + NA ++ +TV GE+LDLA + SD+ LD G SR Sbjct: 65 DKTVVGWWKNGYPKYFGKTPNAPSFIGIGITVNGEKLDLAKVKFSDFELSLDMHQGLLSR 124 Query: 139 QFIWLLGSGKKLKLTFTRLVSMTDSHLGLQQV 170 FI+ G K+K F R + + L +V Sbjct: 125 SFIY-EGKDVKVKFEFERFLHIIQKEAALIKV 155 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 279,051,864 Number of extensions: 10886252 Number of successful extensions: 22711 Number of sequences better than 1.0e-05: 46 Number of HSP's gapped: 22673 Number of HSP's successfully gapped: 46 Length of query: 189 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 70 Effective length of database: 434,140,797 Effective search space: 30389855790 Effective search space used: 30389855790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 122 (51.6 bits)