BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0349.0014.p (77 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060351.1| COG0564: Pseudouridylate synthases, 23S RNA-s... 77 9e-14 ref|ZP_00099063.1| COG0564: Pseudouridylate synthases, 23S RNA-s... 71 7e-12 gb|EAH13683.1| unknown [environmental sequence] 58 5e-08 gb|EAJ04590.1| unknown [environmental sequence] 57 2e-07 gb|EAK61481.1| unknown [environmental sequence] 55 5e-07 >ref|ZP_00060351.1| COG0564: Pseudouridylate synthases, 23S RNA-specific [Clostridium thermocellum ATCC 27405] Length = 243 Score = 77.4 bits (189), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 33/53 (62%), Positives = 46/53 (86%) Query: 19 FEILYEDNHLLGIVKPVNIPVQEDATGDPDLLSLLKEDVKERFHKPGNVYMGL 71 +I++EDNHLL + KPVNIP Q+D + D D+L+LLKED+K+R++KPGNVY+GL Sbjct: 21 IDIIFEDNHLLVVKKPVNIPTQKDKSNDSDMLTLLKEDIKKRYNKPGNVYLGL 73 >ref|ZP_00099063.1| COG0564: Pseudouridylate synthases, 23S RNA-specific [Desulfitobacterium hafniense] Length = 241 Score = 71.2 bits (173), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 33/51 (64%), Positives = 44/51 (86%) Query: 21 ILYEDNHLLGIVKPVNIPVQEDATGDPDLLSLLKEDVKERFHKPGNVYMGL 71 I+YEDNHLL + KP NI Q+D+TGD D+L+LLK+D+K R++KPGNVY+GL Sbjct: 14 IVYEDNHLLVVDKPPNILSQKDSTGDMDILTLLKQDIKIRYNKPGNVYLGL 64 >gb|EAH13683.1| unknown [environmental sequence] Length = 167 Score = 58.2 bits (139), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 30/52 (57%), Positives = 38/52 (73%) Query: 20 EILYEDNHLLGIVKPVNIPVQEDATGDPDLLSLLKEDVKERFHKPGNVYMGL 71 +I+YEDNHLL I K V VQ D TGD LL L+K+ +K+R KPGNV++GL Sbjct: 4 QIVYEDNHLLVINKKVGQLVQGDKTGDDSLLELIKDFIKKRDDKPGNVFLGL 55 >gb|EAJ04590.1| unknown [environmental sequence] Length = 228 Score = 56.6 bits (135), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 26/52 (50%), Positives = 39/52 (75%) Query: 20 EILYEDNHLLGIVKPVNIPVQEDATGDPDLLSLLKEDVKERFHKPGNVYMGL 71 EILYEDNHL+ I K + VQ D TGD L+ ++K+ +K++++K GNV++GL Sbjct: 7 EILYEDNHLIIINKKTGVLVQGDITGDLSLIEIVKDYLKKKYNKKGNVFLGL 58 >gb|EAK61481.1| unknown [environmental sequence] Length = 229 Score = 55.1 bits (131), Expect = 5e-07, Method: Compositional matrix adjust. Identities = 27/52 (51%), Positives = 37/52 (71%) Query: 20 EILYEDNHLLGIVKPVNIPVQEDATGDPDLLSLLKEDVKERFHKPGNVYMGL 71 EIL+EDNHL+ I K I VQ D TGD L+ + K+ +K +++K GNVY+GL Sbjct: 5 EILFEDNHLIIINKKPGILVQGDITGDISLIEVTKDYIKNKYNKKGNVYLGL 56 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.145 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 94,637,628 Number of extensions: 3115396 Number of successful extensions: 6817 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 6818 Number of HSP's successfully gapped: 12 Length of query: 77 Length of database: 757,237,578 Length adjustment: 47 Effective length of query: 30 Effective length of database: 629,627,925 Effective search space: 18888837750 Effective search space used: 18888837750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)