BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0348.0018.p (358 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_790679.1| alcohol dehydrogenase, zinc-containing [Pseudom... 269 9e-71 ref|NP_693674.1| sorbitol dehydrogenase [Oceanobacillus iheyensi... 144 4e-33 gb|EAH89505.1| unknown [environmental sequence] 144 5e-33 ref|NP_927827.1| hypothetical protein [Photorhabdus luminescens ... 140 6e-32 gb|AAO42466.1| xylitol dehydrogenase [Hypocrea jecorina] 139 8e-32 >ref|NP_790679.1| alcohol dehydrogenase, zinc-containing [Pseudomonas syringae pv. tomato str. DC3000] gb|AAO54374.1| alcohol dehydrogenase, zinc-containing [Pseudomonas syringae pv. tomato str. DC3000] Length = 319 Score = 269 bits (687), Expect = 9e-71, Method: Compositional matrix adjust. Identities = 133/324 (41%), Positives = 198/324 (61%), Gaps = 16/324 (4%) Query: 36 LATD-VKVKIQMTGICGTDLAVITGKEPGVPGVIRGHEAVGTVIETGSNVTSLKAGDRVV 94 LA D V++ I ++GICGTDLAV++G+E G G+IRGHEAVG +I+ G +V L+ G RVV Sbjct: 2 LAPDGVEIDIVLSGICGTDLAVLSGREGGEVGIIRGHEAVGIIIDVGKDVVHLQKGMRVV 61 Query: 95 VDPNHSCGKCRFCLQGKLHLCTGTDGQGMPIAGLNKNGTFAPWFVSEEKFLHKLPEQMSW 154 VDPN CG C C K HLC G G+ IAG+NK+GTFA FV+ E+F+++LP+ MSW Sbjct: 62 VDPNEYCGVCEPCRLAKTHLCNGGVNAGLDIAGVNKHGTFAERFVTRERFVYQLPDDMSW 121 Query: 155 EAALATEPLACVLHNFREGRVKTGDSVLILGSGPMGLLCQFVAKRMGCTTVSIEINAGRL 214 A + EP+AC+L+N + ++ G+ VLILGSGPM L+ Q V + MG T++ + N R+ Sbjct: 122 AAGVLVEPVACILNNIDQAFIRAGERVLILGSGPMSLIAQIVLRSMGVDTLATDRNTHRI 181 Query: 215 --AKAQQISDYACPPEELTAERVFRILGEKKFDFVIDTVGTQMEMAEQWVERGGTIIPFG 272 ++Q + EL + ++KFD VIDTVG Q++ A +++ RGG I+ FG Sbjct: 182 QFGRSQSLDVIHADDLELQMQH------QEKFDVVIDTVGNQIDTASRYIGRGGRIVLFG 235 Query: 273 INGRYQYTISPTYYTQHAVRLLGAGEYLNTFEDALQFAKDVPEVASLITRKYSLDQFETT 332 + Y Y + Y+ +A+ ++ AGEY F A++ + +PE+ L+T +Y L+ Sbjct: 236 FDSDYHYMLPVKYFLVNAISIISAGEYNQHFPRAIRLVQKLPELGRLVTHRYVLENHSEV 295 Query: 333 IQELLGNSGNSAVSSEPPVLKSAF 356 LL + + P +KS F Sbjct: 296 FDALLND-------ASAPNIKSVF 312 >ref|NP_693674.1| sorbitol dehydrogenase [Oceanobacillus iheyensis HTE831] dbj|BAC14708.1| sorbitol dehydrogenase (L-iditol 2-dehydrogenase) [Oceanobacillus iheyensis HTE831] Length = 344 Score = 144 bits (362), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 97/323 (30%), Positives = 164/323 (50%), Gaps = 17/323 (5%) Query: 25 NLSEQDIPVITLAT-DVKVKIQMTGICGTDLAVITGKEPGVPGVIRGHEAVGTVIETGSN 83 NL Q++ ++++ VK+K+ GICG+D+ G V GHE G ++E G Sbjct: 13 NLHIQNVEEPSVSSHQVKIKVAYAGICGSDIHTYEGHYKVAAPVTLGHEFSGRIVEVGEG 72 Query: 84 VTSLKAGDRVVVDPNHS-CGKCRFCLQGKLHLCTGTDGQGMPIAGLNKNGTFAPWFVSEE 142 VT K GDRV + S CG+CR+C G +LC G G +NG F + ++ E Sbjct: 73 VTDFKVGDRVTSETTFSICGECRYCQTGDYNLCNHRKG-----LGTQQNGGFTNYLIARE 127 Query: 143 KFLHKLPEQMSWEAALATEPLACVLHNFREGRVKTGDSVLILGSGPMGLLCQFVAKRMGC 202 + LH LP+++ +A TEPLAC H ++ +K GD V++LG GP+GLL VAK G Sbjct: 128 ESLHHLPDEVDDRSASMTEPLACTYHAIQKINIKPGDLVVVLGPGPIGLLAAQVAKSYGA 187 Query: 203 TTV--SIEINAGRLAKAQQIS-DYACP-PEELTAERVFRILGEKKFDFVIDTVGTQMEMA 258 T + ++ + RL KA+++ DY EE E++ + D V++ G+ + A Sbjct: 188 TVMMSGLDNDKIRLDKAKEVGIDYVINIQEEDIKEKIAALTDGYGVDVVLECSGS-IHAA 246 Query: 259 EQWVE---RGGTIIPFGINGRYQYTISPTYYTQHAVRLLGA-GEYLNTFEDALQFAKDVP 314 Q ++ + G+ GI Q + ++G+ + +E +LQ K+ Sbjct: 247 NQGLDLLRKKGSYAQVGIFPDSHVKFDMEKVIQKEIHVVGSRSQKSADWEPSLQLMKEKK 306 Query: 315 -EVASLITRKYSLDQFETTIQEL 336 + +L+T ++++DQ++ Q + Sbjct: 307 VDAKALVTHEFTIDQWDEAYQAI 329 >gb|EAH89505.1| unknown [environmental sequence] Length = 373 Score = 144 bits (362), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 106/359 (29%), Positives = 162/359 (45%), Gaps = 31/359 (8%) Query: 14 MPALVWNSQR-LNLSEQDIPVITLATDVKVKIQMTGICGTDLAVITGKEPGVP------- 65 M AL W+ R + L E D P + DV++ I GICG+DL +P Sbjct: 1 MRALQWHGPRDVRLVEIDPPRVG-PGDVRIAIAYCGICGSDLHEYADGPHAIPVDTPHPL 59 Query: 66 -----GVIRGHEAVGTVIETGSNVTSLKAGDRVVVDPNHSCGKCRFCLQGKLHLCTGTDG 120 + GHE GTV+E G +VT+L+AGDRV V+P + C +C +C G +LC Sbjct: 60 SRRTAPLTLGHEFCGTVVEVGGDVTALRAGDRVAVEPEYRCHQCAYCRSGSYNLCVS--- 116 Query: 121 QGMPIAGLNKNGTFAPWFVSEEKFLHKLPEQMSWEAALATEPLACVLHNFREGRVKTGDS 180 M AGL +G A + V LH+LP+ +S E A EP A LH R G ++ G++ Sbjct: 117 --MGFAGLMGDGGMADFAVVPAYMLHRLPDAVSLEQAAVMEPAAVALHALRRGELRLGET 174 Query: 181 VLILGSGPMGLLCQFVAKRMGCTT-VSIEINAGRLAKAQQIS-----DYACPPEELTAER 234 + G GP+GLL +AK G TT V+++++ RLA A + D P + Sbjct: 175 CAVFGLGPIGLLLIMLAKLQGATTIVAVDVSPERLAAATRFGATHTFDARAPGAPALQDA 234 Query: 235 VFRILGEKKFDFVIDTVG--TQMEMAEQWVERGGTIIPFGINGRYQYTISPTYYTQHAVR 292 + G D + G E A Q + +GG ++ G+ + + + Sbjct: 235 IRAATGGLGVDASFEAAGLPATFEAAMQALRKGGRVVMVGLMPHAGFDAFRAVNDE--LT 292 Query: 293 LLGAGEYLNTFEDALQF-AKDVPEVASLITRKYSL-DQFETTIQELLGNSGNSAVSSEP 349 + Y + +ED L+ A ++ S++TR SL D LL + + P Sbjct: 293 FTASVGYRHAYEDLLRIVASGALDLTSIVTRTVSLEDAVADGFDALLADRAQIKILVSP 351 >ref|NP_927827.1| hypothetical protein [Photorhabdus luminescens subsp. laumondii TTO1] emb|CAE12769.1| unnamed protein product [Photorhabdus luminescens subsp. laumondii TTO1] Length = 353 Score = 140 bits (352), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 104/351 (29%), Positives = 171/351 (48%), Gaps = 24/351 (6%) Query: 14 MPALVWNSQRLNLSEQDIPVITLA--TDVKVKIQMTGICGTDLAVITGKEP-GVPGVIRG 70 M AL +N + ++ QD+ + A DV V I + G+CGTD+ +ITG P +PG G Sbjct: 1 MKALKFN-RVWDVQIQDVEPLRCAEPDDVVVDIAVCGVCGTDVGIITGSYPVAIPGTTLG 59 Query: 71 HEAVGTVIETGSNVTSLKAGDRVVVDPNHSCGKCRFCLQGKLHLCTGTDGQGMPIAGLNK 130 HE G + + G +VT GDRVV++P +SCG CR C G + C G AG++ Sbjct: 60 HETTGVITQIGKSVTRFNVGDRVVINPTYSCGHCRMCQTGSPNHCEKKLGTE---AGVSY 116 Query: 131 NGTFAPWFVSEEKFLHKLPEQMSWEAALATEPLACVLHNFRE-GRVKTGDSVLILGSGPM 189 +G FA ++++E L KL + +S E A TEPL+C L + G T + G+GPM Sbjct: 117 DGAFAEQYLAKESSLIKLDDHVSMEEASLTEPLSCTLTGVDKLGITHTNIRAAVAGAGPM 176 Query: 190 GLLCQFVAKRMGCTTVSIEINAGRLAKAQQISDYACPPE-ELTA---ERVFRILGEKK-- 243 G+L + G +E N R+ A++ PPE EL E + + G+K Sbjct: 177 GMLYIWALHARGVKAFMVEKNESRIQFAKE----NLPPECELYTDFDEALIQKYGDKSAL 232 Query: 244 FDFVIDTVGTQMEMAEQWVERGGTIIPFGINGRYQYTISPTYYTQHAVRLLGAGEYL-NT 302 D +DT G E + + GG ++ + ++ + ++ ++G+ + L N+ Sbjct: 233 IDLCVDTTGQLTEYVFEHLAPGGKLLNVALKDKHA-NLDILKIADKSLSVIGSIDSLNNS 291 Query: 303 FEDALQFAKD-VPEVASLITRKYSLDQFETTIQELLGNSGNSAVSSEPPVL 352 FE A +D V L+++ + + + +Q L + A S+ P+ Sbjct: 292 FERAYTMIRDGVIPAGRLVSQVFDFNDY---LQAFLTVGCDIANKSQKPLF 339 >gb|AAO42466.1| xylitol dehydrogenase [Hypocrea jecorina] Length = 363 Score = 139 bits (351), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 104/332 (31%), Positives = 162/332 (48%), Gaps = 28/332 (8%) Query: 24 LNLSEQDIPVITLATDVKVKIQMTGICGTD--------LAVITGKEPGVPGVIRGHEAVG 75 + E+ P IT DV V + TGICG+D + K+P ++ GHE+ G Sbjct: 24 VTFEERPKPTITDPNDVLVAVNYTGICGSDVHYWVHGAIGHFVVKDP----MVLGHESAG 79 Query: 76 TVIETGSNVTSLKAGDRVVVDPNHSCGKCRFCLQGKLHLCTGTDGQGMPIAGLNKNGTFA 135 TV+E G V SLK GDRV ++P + C +C FC GK +LC P +GT Sbjct: 80 TVVEVGPAVKSLKPGDRVALEPGYPCRRCSFCRAGKYNLCPDMVFAATP----PYHGTLT 135 Query: 136 PWFVSEEKFLHKLPEQMSWEAALATEPLACVLHNFREGRVKTGDSVLILGSGPMGLLCQF 195 + + F +KLP+ +S + EPLA +H ++ RV+ G SV+++G+GP+GLLC Sbjct: 136 GLWAAPADFCYKLPDGVSLQEGALIEPLAVAVHIVKQARVQPGQSVVVMGAGPVGLLCAA 195 Query: 196 VAKRMGCTT-VSIEINAGRLAKAQQI-SDYACPPEELTAERVFRILGEKK-----FDFVI 248 VAK G +T VS++I +L A+ S + + ++AE + + E D VI Sbjct: 196 VAKAYGASTIVSVDIVQSKLDFARGFCSTHTYVSQRISAEDNAKAIKELAGLPGGADVVI 255 Query: 249 DTVGTQ--MEMAEQWVERGGTIIPFGINGRYQYTISPTYYTQHAVRLLGAGEY-LNTFED 305 D G + ++ + V GGT + G+ G+ T V + G+ Y +E Sbjct: 256 DASGAEPSIQTSIHVVRMGGTYVQGGM-GKSDITFPIMAMCLKEVTVRGSFRYGAGDYEL 314 Query: 306 ALQFAKD-VPEVASLITRKYSLDQFETTIQEL 336 A++ + +V LIT S Q E Q++ Sbjct: 315 AVELVRTGRVDVKKLITGTVSFKQAEEAFQKV 346 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 537,175,680 Number of extensions: 22489154 Number of successful extensions: 73187 Number of sequences better than 1.0e-05: 1848 Number of HSP's gapped: 71109 Number of HSP's successfully gapped: 1849 Length of query: 358 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 231 Effective length of database: 412,420,005 Effective search space: 95269021155 Effective search space used: 95269021155 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)