BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0348.0013.p (453 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_347483.1| Probable cation efflux pump (multidrug resistan... 291 2e-77 ref|NP_781140.1| Na+ driven multidrug efflux pump [Clostridium t... 265 2e-69 ref|NP_563421.1| conserved hypothetical protein [Clostridium per... 265 2e-69 ref|NP_971235.1| MATE efflux family protein [Treponema denticola... 211 2e-53 ref|NP_561780.1| conserved hypothetical protein [Clostridium per... 209 8e-53 >ref|NP_347483.1| Probable cation efflux pump (multidrug resistance protein) [Clostridium acetobutylicum] pir||D97004 probable cation efflux pump (multidrug resistance protein) [imported] - Clostridium acetobutylicum gb|AAK78823.1| Probable cation efflux pump (multidrug resistance protein) [Clostridium acetobutylicum] Length = 459 Score = 291 bits (746), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 152/452 (33%), Positives = 253/452 (55%), Gaps = 2/452 (0%) Query: 1 MLKDSKADFYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIIT 60 + D K F+ + +AIP+ +Q+ I++ L L D +++G LG+ A+A VG++ + + ++ Sbjct: 6 LFNDKK--FFKAAFGLAIPIIIQNFILSSLNLVDNIIIGGLGETAIAGVGLANQYFFLLD 63 Query: 61 VVLAGLATGVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFT 120 + L G A+G SI+ AQ+WGK+D K+ + +S IFT+ FP +G F+ Sbjct: 64 LALFGTASGASIFTAQYWGKRDTKNIKRVLGICLITTIILSGIFTIGALAFPNIIIGFFS 123 Query: 121 KDQRLITEGDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLL 180 +D+ +I+ G +L I SYI ++ +S ILR G VK+PM+ S A+ IN LN+ L Sbjct: 124 QDKNVISLGANYLRIICFSYIVTGISFAYSIILRTVGQVKIPMIVSGTALAINTFLNFSL 183 Query: 181 IFGNFGFPEWGIKGAATSTLIARLFECCFIIAAAYKLRLPGVTHIRDWFGAPTALLSKFI 240 ++G F G+ G+A +TLIAR E ++ A YKL+LP + + T + +F Sbjct: 184 VYGIGSFHGIGVYGSAIATLIARFIEFFLMLLAIYKLKLPIAAKLHELLDLSTTFVKRFF 243 Query: 241 RTTYPIVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMI 300 + P++L E +W LG T YA +Y+ MGT I A IT + MI Sbjct: 244 KVAVPVMLNESMWGLGVTAYAAVYAHMGTAVIAATNITSTIDRLSMVVFFGFGNAAAIMI 303 Query: 301 GNKLGADEQDAALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIFQVSEEAHHMGTRLVW 360 GN++G ++ A ++KRFI L F+ + +G+++ +P + + ++ + H ++ Sbjct: 304 GNRIGQKDEKTAFLYAKRFIILSPFIGIFMGIILYTSSPAILYAYNINSQVHMYSKEILH 363 Query: 361 VFAGFLWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSFVWKQPVFLVY 420 + FLW+K+ N GIL SGGD+KF ++ WL+GVP L +K P+ +VY Sbjct: 364 IIGMFLWIKIFNYTNVVGILRSGGDTKFCLMLDMGGMWLVGVPFVALTGIYFKLPIGIVY 423 Query: 421 MILSLEEVVRLSIGLARIHSRKWMKNLVAEIS 452 + +EE V+L +GL R+ S+KW+ NLV++ S Sbjct: 424 YFVFMEEFVKLLVGLPRLLSKKWINNLVSKPS 455 >ref|NP_781140.1| Na+ driven multidrug efflux pump [Clostridium tetani E88] gb|AAO35077.1| Na+ driven multidrug efflux pump [Clostridium tetani E88] Length = 456 Score = 265 bits (677), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 145/446 (32%), Positives = 251/446 (56%) Query: 3 KDSKADFYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIITVV 62 K + ++FY + +A+P+ +Q+ I A L + D +MVG+LG+V +ASVG++ + + ++ Sbjct: 8 KVNNSEFYKVLGRLALPIIIQNFITAFLNMVDTVMVGKLGEVEIASVGVANQYFFFFNLL 67 Query: 63 LAGLATGVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFTKD 122 G+++G I+ +QFWGK+DEK+ + IS++ TL P+ + LF KD Sbjct: 68 ALGISSGGGIFISQFWGKRDEKNIRKILGVNVIAGVTISLLMTLIALLMPETIISLFNKD 127 Query: 123 QRLITEGDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLLIF 182 ++I G +L+ ++SYI +T+ +S LR G +PM S +A+ NI NY LIF Sbjct: 128 PQVIHFGAKYLKTIAISYILTAITLAYSISLRCIGEAIIPMAISGIALITNIFFNYGLIF 187 Query: 183 GNFGFPEWGIKGAATSTLIARLFECCFIIAAAYKLRLPGVTHIRDWFGAPTALLSKFIRT 242 GNFG P G+KGAA +T+IARL EC ++ Y + I++ ++K RT Sbjct: 188 GNFGLPALGVKGAALATVIARLVECVALLIYVYSKKGVLAASIKEMTDINMKFINKIYRT 247 Query: 243 TYPIVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMIGN 302 ++ E W L +YA IY R+GT I ++ I +Q MIGN Sbjct: 248 IASVLFNEACWGLAMVMYAAIYGRIGTKAIASIQICTTVQNLFMVVTLGVANASAVMIGN 307 Query: 303 KLGADEQDAALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIFQVSEEAHHMGTRLVWVF 362 K+G +++ +++K+F K+GI + + +GV +A AP +++F VS+E ++++ Sbjct: 308 KIGEGKEEEGKEYAKKFTKMGIILGIILGVSMAISAPFILNLFNVSQEVMRSSLLILYIT 367 Query: 363 AGFLWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSFVWKQPVFLVYMI 422 + + ++V N ++ G+L GGD+K+ EA WL+G+P L+ +F+ K PV++V + Sbjct: 368 SIIMVMRVFNAVMIVGVLRGGGDAKYALISEAVTMWLVGLPLTLIAAFIIKLPVYVVAGM 427 Query: 423 LSLEEVVRLSIGLARIHSRKWMKNLV 448 +EE + + R+ S KW+KN++ Sbjct: 428 AGIEEFGKFILSYKRVKSNKWIKNVI 453 >ref|NP_563421.1| conserved hypothetical protein [Clostridium perfringens] dbj|BAB82211.1| conserved hypothetical protein [Clostridium perfringens str. 13] Length = 463 Score = 265 bits (676), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 142/447 (31%), Positives = 243/447 (54%) Query: 6 KADFYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIITVVLAG 65 +F + +A+P+T+QS I + L L D LMVG+LG+ ++ASVG++ + I T+ + G Sbjct: 6 NKEFLPTMIMMAVPITIQSFITSSLNLVDNLMVGKLGEESIASVGLANQYIFIFTLCILG 65 Query: 66 LATGVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFTKDQRL 125 + G S++ +QFWGKKD K+ + +S +F L FF + + + D + Sbjct: 66 INAGASVFMSQFWGKKDLKNIKKVLGLDISIGLIVSTLFALGSGFFSYEIMEILSNDLNV 125 Query: 126 ITEGDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLLIFGNF 185 I G +L+I S+S I T +S++LR+T +PM SIV V IN LN++ IFGN Sbjct: 126 IELGAKYLQIVSVSCIFTNFTQAYSSVLRSTEQPSVPMYASIVGVLINAFLNWVFIFGNL 185 Query: 186 GFPEWGIKGAATSTLIARLFECCFIIAAAYKLRLPGVTHIRDWFGAPTALLSKFIRTTYP 245 GF G+ GAA +T IARL E FI++ Y + + + + + + RT++ Sbjct: 186 GFEPMGVNGAALATTIARLVEMIFIVSFVYFRKNKVASRLNELISFDLNFVKIYFRTSWS 245 Query: 246 IVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMIGNKLG 305 +++ E++W LG T Y+I Y+R+GT + +M I L MIGNK+G Sbjct: 246 VIVNEIVWSLGMTAYSIAYARIGTNAVASMQIATTLNNMFMVLLIGLATASSIMIGNKIG 305 Query: 306 ADEQDAALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIFQVSEEAHHMGTRLVWVFAGF 365 ++E++ A +++K + + V +G+LI APL + F VSE + +++ + F Sbjct: 306 SNEEEIAREYAKDIAVIAVLVGFILGILIWITAPLALKPFNVSEGTYLDTVKVLRIIGLF 365 Query: 366 LWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSFVWKQPVFLVYMILSL 425 ++ NM++ G+ GGD+ + ++ WL VP L + V+ PV+ VY ++ Sbjct: 366 FTIRAFNMVMIVGVFRGGGDTTYSMLVQCGTIWLYAVPMAFLGATVFSLPVYGVYFLICT 425 Query: 426 EEVVRLSIGLARIHSRKWMKNLVAEIS 452 EE +++ R+ S KW+KN++++I Sbjct: 426 EEFLKIIFEAVRLKSGKWIKNVISDIE 452 >ref|NP_971235.1| MATE efflux family protein [Treponema denticola ATCC 35405] gb|AAS11116.1| MATE efflux family protein [Treponema denticola ATCC 35405] Length = 460 Score = 211 bits (538), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 122/443 (27%), Positives = 227/443 (51%) Query: 5 SKADFYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIITVVLA 64 S F ++ IA+P+ LQ+ I + + + D +M+G+LG + +A+VG+ +++ ++ ++L Sbjct: 16 SDKSFLKNLFIIAVPIILQNFISSFVNILDTIMIGRLGTIELAAVGLGNQLFFLLNLILY 75 Query: 65 GLATGVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFTKDQR 124 G+ +G ++ AQFWGKKD + S IFTL FP+ L L++KD Sbjct: 76 GIGSGGMVFTAQFWGKKDFNGLQKTFALSLMVAVFFSAIFTLACTIFPKEILSLYSKDAA 135 Query: 125 LITEGDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLLIFGN 184 +I +G +L I++ ++P + IF LR+ V++ + ++V++ +N++LN +LIFG Sbjct: 136 VIEKGVDYLGISAFCFLPFAVNFIFMITLRSIEKVRVAVGATLVSLFVNLILNAVLIFGL 195 Query: 185 FGFPEWGIKGAATSTLIARLFECCFIIAAAYKLRLPGVTHIRDWFGAPTALLSKFIRTTY 244 GFP G+KGAA +T+ +R E + + K + P + +++ F + ++ Sbjct: 196 LGFPALGVKGAAIATVASRAAELIILFSVTKKKKYPILGKLKNHFDFDFKFIRQYFAIVM 255 Query: 245 PIVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMIGNKL 304 P+++ E +W LG T + I++ +GT A IT + +IG K+ Sbjct: 256 PVLINESLWSLGITFHHKIFAGIGTFAYAAYNITNTVSMLTWVIFIGFGNGVSVLIGKKI 315 Query: 305 GADEQDAALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIFQVSEEAHHMGTRLVWVFAG 364 G D A ++ + FV++ +G ++ I+ L F V +L + A Sbjct: 316 GERNYDEAKTYAAKVSIFVPFVAVFVGAMLIPISYLTPIFFNVETVVLQTVMKLFIILAC 375 Query: 365 FLWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSFVWKQPVFLVYMILS 424 +K NM + GI+ +GGD++F + A W + +P +S P + +Y+ L Sbjct: 376 CYPLKAFNMCMLVGIIRAGGDTRFGIICDTAVMWCVSIPLAYSLSVYTSIPAWGIYICLF 435 Query: 425 LEEVVRLSIGLARIHSRKWMKNL 447 EE + +GL RI S KW++++ Sbjct: 436 SEEPFKALLGLWRIKSGKWLRSV 458 >ref|NP_561780.1| conserved hypothetical protein [Clostridium perfringens] dbj|BAB80570.1| conserved hypothetical protein [Clostridium perfringens str. 13] Length = 474 Score = 209 bits (533), Expect = 8e-53, Method: Compositional matrix adjust. Identities = 122/453 (26%), Positives = 237/453 (52%), Gaps = 6/453 (1%) Query: 1 MLKDSKADFYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIIT 60 M+ FY +++IA+P+ +Q+LI ++++ D LMVG LG++ +++V I+ ++ ++T Sbjct: 17 MIITRDKRFYRLLFSIALPIAVQNLITFMVSMVDTLMVGDLGEIQLSAVSIANNLFFVLT 76 Query: 61 VVLAGLATGVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFT 120 +++ GLA G +I +Q+WGK + K+ + I+ IF F P++ +G+FT Sbjct: 77 ILMFGLAGGSNIMISQYWGKGNVKTIHKILAIMYRVCLLITGIFIFIALFLPKYFMGIFT 136 Query: 121 KDQRLITEGDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLL 180 D+ +I G +L I + Y+ +T +LR+ V + ++ ++ +N +LN++ Sbjct: 137 TDKAVIDFGASYLRIVCIGYLFYSITNCTIMMLRSVKTVSISIIVYTASLVVNSILNWIF 196 Query: 181 IFGNFGFPEWGIKGAATSTLIARLFECCFIIAAAYKLRLPGVTHIRDWFGAPTALLSKFI 240 IFGN G PE GI+GAA +T+ AR+ E ++ + I +L ++ Sbjct: 197 IFGNLGAPELGIRGAAIATVCARITEFSIVLVFMFIYERKIGLKIEHLLKLDKEILKDYV 256 Query: 241 RTTYPIVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMI 300 P++ EL+W +G ++ ++I RMGT + A +I +I Sbjct: 257 GLCTPVLCNELLWAIGASMISVIVGRMGTEVVAANSINGVAHQFVTVFIFGMSNATAVII 316 Query: 301 GNKLGADEQDAALDFSKRFIKLGIF-VSLGI--GVLIAAIAPLYVSIFQVSEEAHHMGTR 357 GN +G +++ A +++ +GIF V +G G++I I P V+ + VS + Sbjct: 317 GNTIGEGKKEKAKEYAY---SIGIFSVVMGCISGLMILLIKPFVVNFYNVSYSTKLIAME 373 Query: 358 LVWVFAGFLWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSFVWKQPVF 417 ++ V +G + + G+L GGD+KFV + WL+ +P G ++FV + PV Sbjct: 374 IMTVTSGIIVFQSLASNFMMGVLRGGGDAKFVLINDLIFMWLVAIPGGFFVAFVLELPVA 433 Query: 418 LVYMILSLEEVVRLSIGLARIHSRKWMKNLVAE 450 LV++++ +E+++ + R+ S KW+ ++ + Sbjct: 434 LVFLVIKCDEILKSLTSVYRVISGKWVNDVTKD 466 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.328 0.141 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 572,547,140 Number of extensions: 21317498 Number of successful extensions: 70033 Number of sequences better than 1.0e-05: 570 Number of HSP's gapped: 69572 Number of HSP's successfully gapped: 578 Length of query: 453 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 324 Effective length of database: 406,989,807 Effective search space: 131864697468 Effective search space used: 131864697468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)