BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0348.0002.p (115 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061491.1| hypothetical protein [Clostridium thermocellu... 107 1e-22 ref|ZP_00061713.1| hypothetical protein [Clostridium thermocellu... 92 3e-18 ref|NP_832225.1| S-layer protein / Peptidoglycan endo-beta-N-ace... 78 7e-14 ref|ZP_00181876.1| hypothetical protein Mther016001 [Moorella th... 77 1e-13 ref|ZP_00062117.1| hypothetical protein [Clostridium thermocellu... 77 1e-13 >ref|ZP_00061491.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 4596 Score = 107 bits (266), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 46/110 (41%), Positives = 65/110 (59%) Query: 2 DKIPLWARPYAAQAVSAGIVSGYADGTFRPEQKLTRSELTVMIVRALGIKVDTQAKLTFT 61 D +P WA+PY A A+ G+V GY DG+FRP +LTR E V+++RA GI+ L F Sbjct: 4483 DSVPAWAKPYVATAMHKGVVKGYEDGSFRPSNRLTRQETVVLVLRAFGIEEAQDKTLAFA 4542 Query: 62 DAGDVPKWAAPYIAAAVDKSLVNGTGQNKFAPHRVATRAEAVALIVNLLE 111 D+ +P W+ YI AV+ ++ G N F P R TRAE V +I ++ Sbjct: 4543 DSDKIPAWSRGYIKKAVELGIIKGYSDNTFGPQREITRAEVVTIISKCIQ 4592 Score = 82.4 bits (202), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 42/101 (41%), Positives = 55/101 (54%) Query: 7 WARPYAAQAVSAGIVSGYADGTFRPEQKLTRSELTVMIVRALGIKVDTQAKLTFTDAGDV 66 W + A + IV+GY D T RP +TR+E TVM+V+A G +V LT TD V Sbjct: 4426 WTAEFFADLLRREIVNGYPDRTLRPNAPITRAEATVMVVKAAGFEVSDSIPLTCTDKDSV 4485 Query: 67 PKWAAPYIAAAVDKSLVNGTGQNKFAPHRVATRAEAVALIV 107 P WA PY+A A+ K +V G F P TR E V L++ Sbjct: 4486 PAWAKPYVATAMHKGVVKGYEDGSFRPSNRLTRQETVVLVL 4526 Score = 57.0 bits (136), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 22/50 (44%), Positives = 36/50 (72%) Query: 2 DKIPLWARPYAAQAVSAGIVSGYADGTFRPEQKLTRSELTVMIVRALGIK 51 DKIP W+R Y +AV GI+ GY+D TF P++++TR+E+ +I + + +K Sbjct: 4545 DKIPAWSRGYIKKAVELGIIKGYSDNTFGPQREITRAEVVTIISKCIQLK 4594 >ref|ZP_00061713.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 710 Score = 92.0 bits (227), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 41/105 (39%), Positives = 60/105 (57%) Query: 2 DKIPLWARPYAAQAVSAGIVSGYADGTFRPEQKLTRSELTVMIVRALGIKVDTQAKLTFT 61 D+IP WA Y V AGI++GY D TFRP + LTR E+ V+I++A + +F Sbjct: 585 DQIPAWAAGYVQAGVEAGIIAGYEDNTFRPSRNLTREEMVVLIMKAYEFGAVENPEFSFI 644 Query: 62 DAGDVPKWAAPYIAAAVDKSLVNGTGQNKFAPHRVATRAEAVALI 106 DA ++ W+ P++ AV+ V G N F P + TRAEA ++ Sbjct: 645 DASEIGDWSKPFVGKAVELGFVVGYPDNTFKPKKSVTRAEAFTVL 689 Score = 85.1 bits (209), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 39/105 (37%), Positives = 62/105 (59%) Query: 7 WARPYAAQAVSAGIVSGYADGTFRPEQKLTRSELTVMIVRALGIKVDTQAKLTFTDAGDV 66 WA + A+ + +VSGY DG+ +P+ ++TR+E+ V++V++ G++ L F DA + Sbjct: 528 WAEEFIAKLAARNVVSGYPDGSVKPDIEITRAEMAVIVVKSAGLEPVENVSLKFKDADQI 587 Query: 67 PKWAAPYIAAAVDKSLVNGTGQNKFAPHRVATRAEAVALIVNLLE 111 P WAA Y+ A V+ ++ G N F P R TR E V LI+ E Sbjct: 588 PAWAAGYVQAGVEAGIIAGYEDNTFRPSRNLTREEMVVLIMKAYE 632 >ref|NP_832225.1| S-layer protein / Peptidoglycan endo-beta-N-acetylglucosaminidase [Bacillus cereus ATCC 14579] gb|AAP09426.1| S-layer protein / Peptidoglycan endo-beta-N-acetylglucosaminidase [Bacillus cereus ATCC 14579] Length = 483 Score = 77.8 bits (190), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 41/105 (39%), Positives = 56/105 (53%), Gaps = 2/105 (1%) Query: 4 IPLWARPYAAQAVSAGIVSGYADGTFRPEQKLTRSELTVMIVRALGIKVDTQAKLTFTDA 63 +P WA V +++GY DGTF L R+ T ++ + LGIK+D AK +FTD+ Sbjct: 43 VPTWADKSVNYLVDKQVLNGYPDGTFGSNDSLDRASATKIMTKVLGIKIDPNAKPSFTDS 102 Query: 64 GDVPKWAAPYIAAAVDKSLVNGTGQNKFAPHRVATRAEAVALIVN 108 + WA PYIAAA +V G G F P TRA ++VN Sbjct: 103 QN--HWATPYIAAAEKAGIVKGEGNGIFNPSGKVTRAAMATMLVN 145 Score = 61.2 bits (147), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 36/101 (35%), Positives = 52/101 (51%), Gaps = 5/101 (4%) Query: 7 WARPYAAQAVSAGIVSGYADGTFRPEQKLTRSELTVMIVRALGIK--VDTQAKLTFTDAG 64 WA PY A A AGIV G +G F P K+TR+ + M+V A ++ + ++ F D Sbjct: 106 WATPYIAAAEKAGIVKGEGNGIFNPSGKVTRAAMATMLVNAYKLQSTANHNEQVRFEDLK 165 Query: 65 DVPKWAAPYIAAAVDKSLVNGTGQNKFAPHRVATRAEAVAL 105 W + +D + NGT +N + P+R TRAEA L Sbjct: 166 G--HWGEKHANILIDLKISNGT-ENGWQPNRFITRAEAAQL 203 >ref|ZP_00181876.1| hypothetical protein Mther016001 [Moorella thermoacetica ATCC 39073] Length = 430 Score = 77.0 bits (188), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 39/105 (37%), Positives = 58/105 (55%) Query: 7 WARPYAAQAVSAGIVSGYADGTFRPEQKLTRSELTVMIVRALGIKVDTQAKLTFTDAGDV 66 WA + + G+V+GY DG+FRP+ +TR EL +VR G +V ++ FTD + Sbjct: 223 WAENDINKMAARGLVAGYEDGSFRPDNPVTRVELIAFLVRLAGWRVPAGSQPDFTDRQII 282 Query: 67 PKWAAPYIAAAVDKSLVNGTGQNKFAPHRVATRAEAVALIVNLLE 111 P WA +A A+++ LV+G F P +V +R EA A LE Sbjct: 283 PDWARATVAVALERGLVSGYPDGSFRPGQVVSRVEAAAFFTRYLE 327 >ref|ZP_00062117.1| hypothetical protein [Clostridium thermocellum ATCC 27405] ref|ZP_00062252.1| hypothetical protein [Clostridium thermocellum ATCC 27405] ref|ZP_00062263.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 223 Score = 77.0 bits (188), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 43/113 (38%), Positives = 60/113 (53%), Gaps = 5/113 (4%) Query: 7 WARPYAAQAVSAGIVSGYADGTFRPEQKLTRSELTVMIVRALGIKVDTQA-KLTFTDAGD 65 W A A AG +SGY DGTFRP + +TR E +V++ + + T A + FTD+ Sbjct: 80 WFFADVATAEKAGYISGYPDGTFRPNELITREEASVILCKLINSDPRTHADNIEFTDSDS 139 Query: 66 VPKWAAPYIAAAVDKSLVNGTGQNKFAPHRVATRAEAVALIVN----LLENPI 114 + WA Y+ + V ++ G N F P TRAEAV L+ N L+E PI Sbjct: 140 ISPWAYGYVNSLVYNHIIKGYPDNTFRPKNPVTRAEAVVLLDNALMRLMEPPI 192 Score = 58.5 bits (140), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 30/104 (28%), Positives = 49/104 (47%), Gaps = 1/104 (0%) Query: 7 WARPYAAQAVSAGIVSGYADGTFRPEQKLTRSELTVMIVRALGIKVDTQAKLTFTDAGDV 66 WA+ + G ++GY D +FRP Q +TR+E M R I+ + +FTD + Sbjct: 19 WAQFWIELNAKLGNIAGYEDSSFRPSQSVTRAEFVTMANRFFDIRGSVEFGCSFTDVNEN 78 Query: 67 PKWAAPYIAAAVDKSLVNGTGQNKFAPHRVATRAEAVALIVNLL 110 W +A A ++G F P+ + TR EA ++ L+ Sbjct: 79 -DWFFADVATAEKAGYISGYPDGTFRPNELITREEASVILCKLI 121 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.131 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 160,392,402 Number of extensions: 5334361 Number of successful extensions: 11604 Number of sequences better than 1.0e-05: 133 Number of HSP's gapped: 11397 Number of HSP's successfully gapped: 193 Length of query: 115 Length of database: 757,237,578 Length adjustment: 80 Effective length of query: 35 Effective length of database: 540,029,658 Effective search space: 18901038030 Effective search space used: 18901038030 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)