BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0060.p (139 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_349208.1| Predicted membrane protein [Clostridium acetobu... 93 2e-18 >ref|NP_349208.1| Predicted membrane protein [Clostridium acetobutylicum] pir||A97220 probable membrane protein [imported] - Clostridium acetobutylicum gb|AAK80548.1| Predicted membrane protein [Clostridium acetobutylicum] Length = 418 Score = 93.2 bits (230), Expect = 2e-18, Method: Composition-based stats. Identities = 49/140 (35%), Positives = 80/140 (57%), Gaps = 1/140 (0%) Query: 1 VYVELAILALEQDGFGRESIAAVPMEPITRSISLSESICRAEGVSVIDMAAILGTIFAVL 60 VY+ELAI A+E G +E I ++PM+ L +SI ++G+S+ D+ +IL I + Sbjct: 277 VYLELAITAIEMKGIKKERIMSIPMDKKGEERMLFDSIQSSDGLSLFDLPSILAVIGGIF 336 Query: 61 GASFGFELRWGPIPWGLIGLALGVCASLILFLIAGKGLK-PTAAGTKAVVMLIILCEQGD 119 G+ +GF L+WGPI WGLIG+ C ++ LI + + ++ V+LII C+ Sbjct: 337 GSIYGFVLKWGPILWGLIGIVSCSCVGFVIKLIVTRKYRDEQKCSERSEVVLIIECDDSK 396 Query: 120 SHKAIEILKQFSAISIGKLD 139 +IL + +A+ + KLD Sbjct: 397 VEMVKDILWKNNALGVRKLD 416 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.324 0.141 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 179,929,625 Number of extensions: 6468068 Number of successful extensions: 26443 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 26442 Number of HSP's successfully gapped: 1 Length of query: 139 Length of database: 757,237,578 Length adjustment: 100 Effective length of query: 39 Effective length of database: 485,727,678 Effective search space: 18943379442 Effective search space used: 18943379442 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 120 (50.8 bits)