BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0057.p (361 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAB61395.1| germination protein 119 1e-25 ref|NP_388254.1| gerKB [Bacillus subtilis] >gi|1708044|sp|P49940... 118 2e-25 ref|NP_242802.1| spore germination protein KB [Bacillus halodura... 114 4e-24 ref|NP_149186.1| Spore germination protein, GRKB [Clostridium ac... 80 7e-14 ref|NP_349894.1| Spore germination protein GerKB, membrane prote... 80 9e-14 >gb|AAB61395.1| germination protein Length = 329 Score = 119 bits (298), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 92/325 (28%), Positives = 139/325 (42%), Gaps = 1/325 (0%) Query: 3 KISAYQLXXXXXXXXXXXXXXXXXXXXXXREAWIAELISCSLGVILILIYTALMRMNPGL 62 KISA QL ++AW+A LI LIY L P L Sbjct: 5 KISATQLFILMVLFELGSALLVPLAMGAKQDAWLAILIGMLCSFFFFLIYHKLYSYYPDL 64 Query: 63 SLVEWFPAQLGRWIGTPIAFLYPLLFLYTVGRVVADIRDMVSXXXXXXXXXXXXXGLFVI 122 E+ LG+ +GT +AF+Y + FLY RV+ D +M+ L ++ Sbjct: 65 LPTEYMQKILGKVLGTLLAFIYIVYFLYDASRVLRDFGEMLLTFAYPDTPLFIANALLML 124 Query: 123 IIAYCVYGGALIIARLGEIFXXXXXXXXXXXXXXXXXXGVMHIRNIQPVMEHGFGPIWNV 182 +I Y G IAR GE+ G++ +N+QPV++ G P+ V Sbjct: 125 VIIYITRKGIEAIARSGELLFIFMYFLAIAGFILIISSGLIDFKNLQPVLKEGILPVAKV 184 Query: 183 VYPAGITQPFGETLALAMIWPLSKTPSKVMKITVLSTLLSGIMIACLDIFVISVFG-GVF 241 + PF E + M+ P P K + +T LSGI + I ISV G + Sbjct: 185 ALTETLYFPFTEAIVFTMLLPYLSNPKKANVTMLCATGLSGINLIITMIINISVLGVDLT 244 Query: 242 SRFLYPLYTLLSLISVGKFIENLQMFGVLYFLMTALLKGAIYMYAALKGVQQLTKLKSYH 301 +R +PL + + I V F+E L +F + ++ K +I YA++ L K+KS Sbjct: 245 TRSQFPLLSTVQTIQVADFLERLDVFFMFAMVIGIYFKVSILFYASIISTATLFKIKSPS 304 Query: 302 TLMIPGCIIAWLLGMTMSRNIAEHI 326 L P II L +T++ N EH+ Sbjct: 305 RLAYPLGIIVLFLSITIASNFQEHL 329 >ref|NP_388254.1| gerKB [Bacillus subtilis] sp|P49940|GRKB_BACSU Spore germination protein KB pir||I39860 germination response protein gerKB - Bacillus subtilis dbj|BAA11256.1| GerKB [Bacillus subtilis] dbj|BAA09004.1| spore germination protein GerKB [Bacillus subtilis] emb|CAB12180.1| gerKB [Bacillus subtilis subsp. subtilis str. 168] Length = 373 Score = 118 bits (295), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 78/296 (26%), Positives = 140/296 (47%), Gaps = 1/296 (0%) Query: 32 REAWIAELISCSLGVILILIYTALMRMNPGLSLVEWFPAQLGRWIGTPIAFLYPLLFLYT 91 R+AWIA L+ C++G+ L +Y + + P S E+ LG + +FLY L F Y Sbjct: 34 RDAWIAVLLGCAIGLFLFYLYQGIYQCYPNSSPKEYMDDMLGTKLSWLFSFLYILYFAYI 93 Query: 92 VGRVVADIRDMVSXXXXXXXXXXXXXGLFVIIIAYCVYGGALIIARLGEIFXXXXXXXXX 151 RV+ D +M+ L +++ Y V G ++AR E+ Sbjct: 94 AARVLRDFGEMLLTFAYHDTPIIIVNALLMVVSIYAVRKGIEVLARAAELLFGAMYLLGA 153 Query: 152 XXXXXXXXXGVMHIRNIQPVMEHGFGPIWNVVYPAGITQPFGETLALAMIWPLSKTPSKV 211 G + N++PV+ +G P+ + V+ + PFGE + MI+P V Sbjct: 154 IGLVLIIVSGTIDPHNLKPVLANGISPVLHSVFTQTMYVPFGEVVLFVMIFPNLNDRKDV 213 Query: 212 MKITVLSTLLSGIMIACLDIFVISVFGGVFS-RFLYPLYTLLSLISVGKFIENLQMFGVL 270 K+ +++ +SG+++A ISV + R +PL + + I V +F++ L +F +L Sbjct: 214 KKMGMIAMAISGLIVALTVAINISVLDVDLTLRSQFPLLSTIQTIKVEEFLDRLDVFFML 273 Query: 271 YFLMTALLKGAIYMYAALKGVQQLTKLKSYHTLMIPGCIIAWLLGMTMSRNIAEHI 326 ++ K ++Y+YA + G L K K+ L P + +L +T++ N +EH+ Sbjct: 274 ALIIGGFFKVSLYLYATVVGTSTLFKEKNPSQLAYPMGLGILILSITIATNFSEHL 329 >ref|NP_242802.1| spore germination protein KB [Bacillus halodurans] pir||H83891 spore germination protein KB gerKB [imported] - Bacillus halodurans (strain C-125) dbj|BAB05655.1| spore germination protein KB [Bacillus halodurans] Length = 373 Score = 114 bits (285), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 84/328 (25%), Positives = 155/328 (47%), Gaps = 4/328 (1%) Query: 32 REAWIAELISCSLGVILILIYTALMRMNPGLSLVEWFPAQLGRWIGTPIAFLYPLLFLYT 91 ++AW+ LI+ G++L + L P + + + L IG I FLY L F+Y Sbjct: 35 QDAWMTVLIAMLAGLVLYCVLYYLHSHFPSDTWIAYLQKVLTVPIGWFIGFLYCLYFIYI 94 Query: 92 VGRVVADIRDMVSXXXXXXXXXXXXXGLFVIIIAYCVYGGALIIARLGEIFXXXXXXXXX 151 RV+ D D+++ L V +I Y ++ G ++AR GE F Sbjct: 95 ATRVLRDFGDLLTTSMLTDTPLMVINLLMVFVIVYVLFHGINVLARTGEFFLIILFFIGF 154 Query: 152 XXXXXXXXXGVMHIRNIQPVMEHGFGPIWNVVYPAGITQPFGETLALAMIWPLSKTPSKV 211 + +I+N+ PV+E+G+ P+ +P T PFGE + M++ Sbjct: 155 FSVTLIFSTDLYNIKNLMPVLENGWMPVLREAFPVTFTFPFGEIIVFTMLFHFLNKKEMG 214 Query: 212 MKITVLSTLLSGIMIACLDIFVISVFG-GVFSRFLYPLYTLLSLISVGKFIENLQMFGVL 270 ++I +L+ +SG+++A I I V G + +R +PL T++ I FIE L V Sbjct: 215 LRIGLLAIGMSGLLLALTTILYIIVLGVDITNRATFPLLTMVGKIQAADFIERLDALAVT 274 Query: 271 YFLMTALLKGAIYMYAALKGVQQLTKLKSYHTLM-IPGCIIAWLLGMTMSRNIAEHIYYH 329 ++ K AI+ YA + G +L K+ + L+ + G I+ + + ++ N +EH+ Sbjct: 275 VLVVAMFFKVAIFFYAGVAGFSELFKVDKFQKLLPLFGAILVF-NSVHIAANFSEHL-KE 332 Query: 330 HFKILVPYVWVPLFIILPTVLLLVAWFR 357 +++ Y+ +P + +P ++L + R Sbjct: 333 GLEVVPYYLHLPFQVGIPLIVLAIVLIR 360 >ref|NP_149186.1| Spore germination protein, GRKB [Clostridium acetobutylicum] gb|AAK76768.1| Spore germination protein, GRKB [Clostridium acetobutylicum] Length = 361 Score = 80.1 bits (196), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 65/295 (22%), Positives = 120/295 (40%), Gaps = 2/295 (0%) Query: 32 REAWIAELISCSLGVILILIYTALMRMNPGLSLVEWFPAQLGRWIGTPIAFLYPLLFLYT 91 + AWI L++ +G+ ++ Y L + P LVE F LG+ +G P+ LY + F++ Sbjct: 32 QNAWIVVLLALLIGLFILWSYLELQKAFPTKGLVEIFTNILGKPLGLPLCILYAMGFIWG 91 Query: 92 VGRVVADIRDMVSXXXXXXXXXXXXXGLFVIIIAYCVYGGALIIARLGEIFXXXXXXXXX 151 GR + + ++++ F+ + Y G + AR E Sbjct: 92 CGRNLREFIELIAMTVIPHTPLWIILFFFIGLSLYVSVKGFEVFARTTEFIFPTIIFFLI 151 Query: 152 XXXXXXXXXGVMHIRNIQPVMEHGFGPIWNVVYPAGITQPFGETLALAMIWPLSKTPSKV 211 G +H +N+ P++ G PI + P + PFGE M W + + Sbjct: 152 ILFALISISGEVHFKNLTPILGDGLKPILKSL-PGVVWFPFGEIYVFLMYWHYVNDINTI 210 Query: 212 MKITVLSTLLSGIMIACLDIFVISVFGGVFSRF-LYPLYTLLSLISVGKFIENLQMFGVL 270 K + + +LSGI++ + I G ++ PL L LI++G I +L + GV+ Sbjct: 211 KKASFKAVILSGILLCFTTMINICTLGAKYTSIATIPLVETLRLINIGDIITHLDVIGVI 270 Query: 271 YFLMTALLKGAIYMYAALKGVQQLTKLKSYHTLMIPGCIIAWLLGMTMSRNIAEH 325 + K +Y+ + + +K ++I I + + A H Sbjct: 271 IIFLGGFFKMTLYLNGIAIIINSVFSIKKSQLVLILTGIFMMYFSIYFEPSFAYH 325 >ref|NP_349894.1| Spore germination protein GerKB, membrane protein [Clostridium acetobutylicum] pir||G97305 spore germination protein GerKB, membrane protein [imported] - Clostridium acetobutylicum gb|AAK81234.1| Spore germination protein GerKB, membrane protein [Clostridium acetobutylicum] Length = 366 Score = 79.7 bits (195), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 63/270 (23%), Positives = 125/270 (46%), Gaps = 3/270 (1%) Query: 32 REAWIAELISCSLGVILILIYTALMRMNPGLSLVEWFPAQLGRWIGTPIAFLYPLLFLYT 91 + AWI L+ +I Y L + + S++++ P G+++G+ +F Y F+YT Sbjct: 33 QNAWIGVLMCIIPALIFQSTYIYLWQNHKDDSIIQYMPKIWGKFLGSIFSFFYLTFFIYT 92 Query: 92 VGRVVADIRDMVSXXXXXXXXXXXXXGLFVIIIAYCVYGGALIIARLGEIFXXXXXXXXX 151 RV+ ++ ++ + + + Y VY G I+RL +F Sbjct: 93 ASRVLRNLSVLIITSSMPRMPIFLICMILMFVCWYGVYLGIENISRLMYVFLFAWISFFA 152 Query: 152 XX-XXXXXXXGVMHIRNIQPVMEHGFGPIWNVVYPAGITQPFGETLALAMIWPLSKTPSK 210 + +++ P++E+G P+ I PFGE + M++PL K Sbjct: 153 FEWIFFIIPPNQIEPKHMFPILENGIVPVIKKSLSV-IAFPFGENIISTMLFPLVSDQKK 211 Query: 211 VMKITVLSTLLSGIMIACLDIFVISVFGGVFS-RFLYPLYTLLSLISVGKFIENLQMFGV 269 + K + + ++ GI+I+ + I V G FS L+P L ++ V ++ + L +F + Sbjct: 212 LKKYALSAVIVQGILISLSTLLCILVLGLKFSISALFPFLQTLRVLKVTEYFDRLDIFAI 271 Query: 270 LYFLMTALLKGAIYMYAALKGVQQLTKLKS 299 + ++ + K +++ Y A+KG QL K+K+ Sbjct: 272 IIVVIVSFTKVSVFCYGAVKGTAQLFKIKN 301 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.332 0.147 0.464 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 425,641,175 Number of extensions: 14851260 Number of successful extensions: 51661 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 51641 Number of HSP's successfully gapped: 23 Length of query: 361 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 234 Effective length of database: 412,420,005 Effective search space: 96506281170 Effective search space used: 96506281170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 126 (53.1 bits)