BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0049.p (1118 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAO88279.1| endo-beta-N-acetylglucosaminidase [Bacillus circu... 196 2e-48 ref|NP_623945.1| S-layer homology domain [Thermoanaerobacter ten... 177 2e-42 ref|NP_624131.1| Subtilisin-like serine proteases [Thermoanaerob... 169 3e-40 sp|P38536|APU_THETU Amylopullulanase precursor (Alpha-amylase/pu... 165 6e-39 gb|AAB00841.1| pullulanase [Thermoanaerobacterium thermosulfurig... 165 6e-39 >gb|AAO88279.1| endo-beta-N-acetylglucosaminidase [Bacillus circulans] Length = 1936 Score = 196 bits (499), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 124/353 (35%), Positives = 186/353 (52%), Gaps = 14/353 (3%) Query: 768 VEITLGQGQARASLPVNAGALLGTRALVIRDGEASLQIPASVLTELGTKLAEAKRSGAAI 827 V I + G LP A L + ++ +L++P+ +L +L +++A+ S I Sbjct: 1590 VTIVVPAGATEIVLPPQAAELPQQHKVELKTDRVTLEVPSGLLKKLASRIADKDVS---I 1646 Query: 828 MIKLAPVPEGAAP---AGNGS---GIYRQEGTAYEFGLVLRAADGSEITLSSFSQPVRAM 881 +K AP+ A + N S G Y+F L A+GS LS F QP+ Sbjct: 1647 SLKAAPLTAAQAKDAISKNKSVSPSAITLAGGVYDFKLSAAGANGSYAELSEFDQPITIS 1706 Query: 882 LPYADSAVNVELLGVYLKHGDTSQWSYVGGTADKTSKKVVLMLDHFSTYAVLSYDATYTD 941 L +S VN E +G+Y G+ + Y+GG + ++ + HFS YAVL + D Sbjct: 1707 LK-IESGVNPEQVGIYYISGN-GKLDYIGG--EYRDGELAAEVTHFSQYAVLKVVKVFDD 1762 Query: 942 VPPDHWAFAALKSLSAKHVVTGLPDSLFAPAQTTTRAEFTAMLVRALNLKSTGGQLPFQD 1001 VP HWA + L+++ +V G ++ F P + TRAEFTA+L RAL L + GG F D Sbjct: 1763 VPAGHWAEGVISKLTSRLMVDGTSETTFEPERVVTRAEFTALLARALKL-TAGGTPTFAD 1821 Query: 1002 VQDGSWYTGSVAAAYEAGLVQGVSATAFAPDARMTREQMAVLLIKAYEYKQGQAASRGGL 1061 V+ G WY +V AA EAG+ +G SA F P AR+TRE+M V+ ++AY + + S G Sbjct: 1822 VKAGDWYADAVTAAVEAGIAEGKSAGQFEPQARITREEMVVMTMRAYNKAKDKGPSTGVE 1881 Query: 1062 KNLSDSALISVWAKAQVDQAVAHGMMQGTGDGRFDPQGPVSRAQGAQAMYNFL 1114 + +D IS WA QV A A ++QG G+F+PQG +RA+ Q ++N L Sbjct: 1882 ASFTDENQISAWAVEQVKAAAALQLIQGRAQGKFEPQGTATRAEAVQVIFNML 1934 >ref|NP_623945.1| S-layer homology domain [Thermoanaerobacter tengcongensis] gb|AAM25549.1| S-layer homology domain [Thermoanaerobacter tengcongensis] Length = 1321 Score = 177 bits (448), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 103/339 (30%), Positives = 168/339 (49%), Gaps = 17/339 (5%) Query: 781 LPVNAGALL--GTRALVIRDGEASLQIPASVLTELGTKLAEAKRSGAAIMIKLAPVPEGA 838 +PV+ L+ + ++++ E +L+ + VL L + G+ + + + + Sbjct: 995 IPVSVLNLIAENQKDVIVKSDEIALKFESKVLAVSKEVLDLINKEGSITL----TIEKKS 1050 Query: 839 APAGNGSGIYRQEGTAYEFGLVLRAADGSEITLSSFSQPVRAMLPYADSAVNVELLGVYL 898 P N Y+ AY+ + A S+I L S PV+ D A ++ +GVY Sbjct: 1051 KPEANA---YKPVSNAYDITI---KAGESKIKLES---PVKLTFEVKD-AKDIRKVGVYY 1100 Query: 899 KHGDTSQWSYVGGTADKTSKKVVLMLDHFSTYAVLSYDATYTDVPPDHWAFAALKSLSAK 958 + T +W YVGG DK + V + HFSTY YD + DVP D WA+ + L++K Sbjct: 1101 LNEATGKWEYVGGKVDKNTNTVTAEVKHFSTYGAFEYDKQFKDVPKDFWAYDVINVLASK 1160 Query: 959 HVVTGLPDSLFAPAQTTTRAEFTAMLVRALNLKSTGGQLPFQDVQDGSWYTGSVAAAYEA 1018 H++ G+ D FAP TRAEF A+++RAL + + F+DV+ G+WY ++ AAY A Sbjct: 1161 HIIKGMDDDNFAPNAKITRAEFAALMIRALGIPEEPYKGEFEDVKAGAWYANAIEAAYRA 1220 Query: 1019 GLVQGVSATAFAPDARMTREQMAVLLIKAYEYKQGQAASRGGLKNLSDSALISVWAKAQV 1078 G++ G PD +TRE+MA ++++ Y G G D+ IS WA+ V Sbjct: 1221 GIMMG-DGKKMRPDDPITREEMAAVIMRVYAKMTGYKEENIGNTVFKDNDSISEWARNVV 1279 Query: 1079 DQAVAHGMMQGTGDGRFDPQGPVSRAQGAQAMYNFLRAV 1117 A G+++G D F + +RA+G +Y L + Sbjct: 1280 ANAAKLGIVKGYEDNTFRAKNNATRAEGVTLLYRILEKL 1318 >ref|NP_624131.1| Subtilisin-like serine proteases [Thermoanaerobacter tengcongensis] gb|AAM25735.1| Subtilisin-like serine proteases [Thermoanaerobacter tengcongensis] Length = 1999 Score = 169 bits (429), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 95/327 (29%), Positives = 157/327 (48%), Gaps = 21/327 (6%) Query: 792 RALVIRDGEASLQIPASVLTELGTKLAEAKRSGAAIMI---KLAPVPEGAAPAGNGSGIY 848 + +V++ GE +L I +T L ++G+ ++ K V P N I Sbjct: 1686 KNVVVKSGEVALSIDPKAMTVSEETLNLINKTGSVKLVVEDKGKKVANSLIPVSNAYDIT 1745 Query: 849 RQEGTAYEFGLVLRAADGSEITLSSFSQPVRAMLPYADSAVNVELLGVYLKHGDTSQWSY 908 + G ++I L S PV+ D ++ +G+Y + T +W Y Sbjct: 1746 IKTGE-------------NKIKLDS---PVKLTFEVKDVK-DIRKVGIYYLNEVTGKWEY 1788 Query: 909 VGGTADKTSKKVVLMLDHFSTYAVLSYDATYTDVPPDHWAFAALKSLSAKHVVTGLPDSL 968 VGG DK + + HFSTY YD + DV D W + + L+++H++ G+ + Sbjct: 1789 VGGKIDKNANTITFEAKHFSTYGAFEYDKQFKDVEKDFWGYDVINVLASRHIIKGMTEDT 1848 Query: 969 FAPAQTTTRAEFTAMLVRALNLKSTGGQLPFQDVQDGSWYTGSVAAAYEAGLVQGVSATA 1028 FAP TRAEF A+++RAL + + F+DV++G WY ++ AAY+ G++ G Sbjct: 1849 FAPNAKITRAEFAALMIRALGIPEEPYKGEFEDVKEGMWYANAIEAAYQEGIMLG-DGKK 1907 Query: 1029 FAPDARMTREQMAVLLIKAYEYKQGQAASRGGLKNLSDSALISVWAKAQVDQAVAHGMMQ 1088 PD +TRE+MA ++++ Y G G +D+ IS WA+ V AV G+++ Sbjct: 1908 MRPDDPITREEMAAVIMRVYSKLAGYKEENIGNTTFADNNKISEWARNVVANAVKLGIVR 1967 Query: 1089 GTGDGRFDPQGPVSRAQGAQAMYNFLR 1115 G D F P+ +RA+ A +Y L Sbjct: 1968 GYDDNTFKPKDNATRAEAAAMLYRILE 1994 >sp|P38536|APU_THETU Amylopullulanase precursor (Alpha-amylase/pullulanase) (Pullulanase type II) [Includes: Alpha-amylase (1,4-alpha-D-glucan glucanohydrolase); Pullulanase (1,4-alpha-D-glucan glucanohydrolase) (Alpha-dextrin endo-1,6-alpha-glucosidase)] Length = 1861 Score = 165 bits (418), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 90/254 (35%), Positives = 137/254 (53%), Gaps = 9/254 (3%) Query: 868 EITLSSFS------QPVRAMLPYADSAVNVELLGVYLKHGDTSQWSYVGGTADKTSKKVV 921 +IT+S S +PV L + A + + VY + T+QW YVGG D +S + Sbjct: 1606 DITISGISGNVTLAKPVEVTLNIS-KANDPRKVAVYYYNPTTNQWEYVGGKVDASSGTIT 1664 Query: 922 LMLDHFSTYAVLSYDATYTDVPPDHWAFAALKSLSAKHVVTGLPDSLFAPAQTTTRAEFT 981 HFS YA YD T+ D+ D+WA ++ L+++H+V G+ D+ + P +T TRAEFT Sbjct: 1665 FNATHFSQYAAFEYDKTFNDIK-DNWAKDVIEVLASRHIVEGMTDTQYEPNKTVTRAEFT 1723 Query: 982 AMLVRALNLKSTGGQLPFQDVQDGSWYTGSVAAAYEAGLVQGVSATAFAPDARMTREQMA 1041 AM++R LN+K F DV+ G WY ++ AAY+AG+++G A P+ +TRE+M Sbjct: 1724 AMILRLLNIKDETYSGEFSDVKSGDWYANAIEAAYKAGIIEGDGKNA-RPNDSITREEMT 1782 Query: 1042 VLLIKAYEYKQGQAASRGGLKNLSDSALISVWAKAQVDQAVAHGMMQGTGDGRFDPQGPV 1101 + ++AYE G SD IS WA+ V A G++ G + F P+G Sbjct: 1783 AIAMRAYEMLTQYKEENIGATTFSDDKSISDWARNVVANAAKLGIVNGEPNNVFAPKGNA 1842 Query: 1102 SRAQGAQAMYNFLR 1115 +RA+ A +Y L Sbjct: 1843 TRAEAAAIIYGLLE 1856 >gb|AAB00841.1| pullulanase [Thermoanaerobacterium thermosulfurigenes] Length = 1861 Score = 165 bits (418), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 90/254 (35%), Positives = 137/254 (53%), Gaps = 9/254 (3%) Query: 868 EITLSSFS------QPVRAMLPYADSAVNVELLGVYLKHGDTSQWSYVGGTADKTSKKVV 921 +IT+S S +PV L + A + + VY + T+QW YVGG D +S + Sbjct: 1606 DITISGISGNVTLAKPVEVTLNIS-KANDPRKVAVYYYNPTTNQWEYVGGKVDASSGTIT 1664 Query: 922 LMLDHFSTYAVLSYDATYTDVPPDHWAFAALKSLSAKHVVTGLPDSLFAPAQTTTRAEFT 981 HFS YA YD T+ D+ D+WA ++ L+++H+V G+ D+ + P +T TRAEFT Sbjct: 1665 FNATHFSQYAAFEYDKTFNDIK-DNWAKDVIEVLASRHIVEGMTDTQYEPNKTVTRAEFT 1723 Query: 982 AMLVRALNLKSTGGQLPFQDVQDGSWYTGSVAAAYEAGLVQGVSATAFAPDARMTREQMA 1041 AM++R LN+K F DV+ G WY ++ AAY+AG+++G A P+ +TRE+M Sbjct: 1724 AMILRLLNIKEETYSGEFSDVKSGDWYANAIEAAYKAGIIEGDGKNA-RPNDSITREEMT 1782 Query: 1042 VLLIKAYEYKQGQAASRGGLKNLSDSALISVWAKAQVDQAVAHGMMQGTGDGRFDPQGPV 1101 + ++AYE G SD IS WA+ V A G++ G + F P+G Sbjct: 1783 AIAMRAYEMLTQYKEENIGATTFSDDKSISDWARNVVANAAKLGIVNGEPNNVFAPKGNA 1842 Query: 1102 SRAQGAQAMYNFLR 1115 +RA+ A +Y L Sbjct: 1843 TRAEAAAIIYGLLE 1856 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.131 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,521,279,025 Number of extensions: 62807059 Number of successful extensions: 108267 Number of sequences better than 1.0e-05: 182 Number of HSP's gapped: 107816 Number of HSP's successfully gapped: 209 Length of query: 1118 Length of database: 757,237,578 Length adjustment: 136 Effective length of query: 982 Effective length of database: 387,984,114 Effective search space: 381000399948 Effective search space used: 381000399948 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)