BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0034.p (169 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_420565.1| glycosyl hydrolase, family 30 [Caulobacter cres... 110 2e-23 ref|NP_823885.1| putative glycosyl hydrolase [Streptomyces averm... 84 1e-15 ref|NP_812224.1| glucosylceramidase precursor [Bacteroides theta... 79 5e-14 ref|NP_636452.1| glycosyl hydrolase [Xanthomonas campestris pv. ... 75 7e-13 ref|NP_641512.1| glycosyl hydrolase [Xanthomonas axonopodis pv. ... 70 2e-11 >ref|NP_420565.1| glycosyl hydrolase, family 30 [Caulobacter crescentus CB15] pir||A87467 glycosyl hydrolase, family 30 CC1757 [imported] - Caulobacter crescentus gb|AAK23733.1| glycosyl hydrolase, family 30 [Caulobacter crescentus CB15] Length = 469 Score = 110 bits (274), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 56/133 (42%), Positives = 82/133 (61%), Gaps = 3/133 (2%) Query: 1 VKLAFEDKEVHFTEGSGGEWIPPFEQAFSNVI-RTGIEILRNHSKSFVLWNMALDEQNGP 59 V+ A+ DKEV FTE SGGEW P F+ +F+ ++ + I R ++ ++WN+ALDE+ GP Sbjct: 308 VRAAYPDKEVFFTECSGGEWAPKFDDSFAWMVEQLIIGSTRGGARGVLMWNLALDEKFGP 367 Query: 60 AVPGFGRSTCRGIVRIDQQTRELTYTLDYYALAHFSKVIRPKALRLESHSTHPAVTTAAF 119 G G CRG+V ID QT LT T +YYA H S+ ++P A+R+ S + + T AF Sbjct: 368 HAGGCG--DCRGVVSIDSQTGALTRTQEYYAFGHASRFVKPDAVRIGSPAKVEGLRTVAF 425 Query: 120 RNLDGSVGAVLFN 132 +N DG ++ N Sbjct: 426 QNPDGQRVLIVLN 438 >ref|NP_823885.1| putative glycosyl hydrolase [Streptomyces avermitilis MA-4680] dbj|BAC70420.1| putative glycosyl hydrolase [Streptomyces avermitilis MA-4680] Length = 647 Score = 83.6 bits (205), Expect = 1e-15, Method: Composition-based stats. Identities = 52/154 (33%), Positives = 85/154 (55%), Gaps = 12/154 (7%) Query: 13 TEGSGGEWIPPFEQA-FSNVIRTGIEILRNHSKSFVLWNMALDEQNGPAVPGFGRSTCRG 71 TE SGG WI ++ SN+I + RN +KS W++A+D+ GP G G TC G Sbjct: 364 TEHSGGTWIANQQREDMSNII----DYTRNWAKSVTKWSLAVDQNMGPHNGGCG--TCTG 417 Query: 72 IVRI---DQQTRELTYTLDYYALAHFSKVIRPKALRLESHSTHPAVTTAAFRNLDGSVGA 128 +V + D + + YT++YY + H +K +RP A R+ S ++ +V A+RN DGS Sbjct: 418 LVTVHNGDGASGTVDYTVEYYTMGHLTKFVRPGAQRVASTAS-ASVPNVAWRNPDGSKAL 476 Query: 129 VLFNDGEELETVAVRLPGEEPLTFSMAPKSAVSL 162 + +ND +TV + G + T+S+ K++ + Sbjct: 477 IAYNDASTAKTVTLNW-GSQHATYSLPGKTSATF 509 >ref|NP_812224.1| glucosylceramidase precursor [Bacteroides thetaiotaomicron VPI-5482] gb|AAO78418.1| glucosylceramidase precursor [Bacteroides thetaiotaomicron VPI-5482] Length = 496 Score = 78.6 bits (192), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 49/166 (29%), Positives = 81/166 (48%), Gaps = 7/166 (4%) Query: 1 VKLAFEDKEVHFTEGSGGEWIPPFE---QAFSNVIRTGIEILRNHSKSFVLWNMALDEQN 57 + A+ +KE+ FTE S G W + + ++ + + N K ++WN+ LD Sbjct: 326 IHQAYPEKELLFTETSIGTWNSGRDLSKRLMEDMEEVALGTINNWCKGVIVWNLMLDNDR 385 Query: 58 GPAVPGFGRSTCRGIVRIDQQT-RELTYTLDYYALAHFSKVIRPKALRLESHS-THPAVT 115 GP G G TC G V I+ + + YY +AH S V++P A+R+ + T +T Sbjct: 386 GPNREG-GCQTCYGAVDINNSDYKTIIRNSHYYIIAHLSSVVKPGAVRIATTGYTDNGIT 444 Query: 116 TAAFRNLDGSVGAVLFNDGEELETVAVRLPGEEPLTFSMAPKSAVS 161 +AF N DG+ VL N+ E+ + + V G+ + + KS S Sbjct: 445 CSAFENTDGTYAFVLINNNEKSKKITVS-DGQRHFAYDVPGKSVTS 489 >ref|NP_636452.1| glycosyl hydrolase [Xanthomonas campestris pv. campestris str. ATCC 33913] gb|AAM40376.1| glycosyl hydrolase [Xanthomonas campestris pv. campestris str. ATCC 33913] Length = 548 Score = 74.7 bits (182), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 51/146 (34%), Positives = 74/146 (50%), Gaps = 10/146 (6%) Query: 1 VKLAFEDKEVHFTEGSGGEWIPPFEQAFSNVIRTGIEILR--NHSK-SFVLWNMALDEQN 57 V A+ DK + TE + ++ P Q + N R G I+ NH + WN+ LDE Sbjct: 384 VAQAYPDKHLLLTEAAVEKFDPAKLQHWPNGERYGTAIINDLNHGAVGWTDWNILLDEHG 443 Query: 58 GPAVPGFGRSTCRGIVRIDQQTRELTYTLDYYALAHFSKVIRPKALRLESHSTHPAVTTA 117 GP G + C V + +T E+ YT Y+ + HFSK IRP A R+ + S+ + T Sbjct: 444 GPNHVG---NYCFAPVHANTRTGEVIYTPSYWYIGHFSKFIRPGAQRVSAASSRSNLATT 500 Query: 118 AFRNLDGSVGAVLFNDGEELETVAVR 143 AF N DGS+ V+ N + VA+R Sbjct: 501 AFVNSDGSLATVVMNATD----VAIR 522 >ref|NP_641512.1| glycosyl hydrolase [Xanthomonas axonopodis pv. citri str. 306] gb|AAM36048.1| glycosyl hydrolase [Xanthomonas axonopodis pv. citri str. 306] Length = 458 Score = 70.1 bits (170), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 50/138 (36%), Positives = 71/138 (51%), Gaps = 6/138 (4%) Query: 1 VKLAFEDKEVHFTEGSGGEWIPPFEQAFSNVIRTGIEILR--NHSK-SFVLWNMALDEQN 57 V A+ DK + TE + ++ P Q + N R G I+ NH + WN+ LD+ Sbjct: 294 VAQAYPDKHLLLTEAAVEKFDPAKLQYWPNGERYGTAIINDLNHGAVGWTDWNILLDQHG 353 Query: 58 GPAVPGFGRSTCRGIVRIDQQTRELTYTLDYYALAHFSKVIRPKALRLESHSTHPAVTTA 117 GP G + C V D +T E+ YT Y+ + HFSK IRP A R+ S S+ + T Sbjct: 354 GPNHVG---NYCFAPVHADTRTGEVIYTPSYWYIGHFSKFIRPGARRVSSASSRSNLATT 410 Query: 118 AFRNLDGSVGAVLFNDGE 135 AF N DGS+ V+ N G+ Sbjct: 411 AFLNTDGSLAMVVMNAGD 428 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 253,223,374 Number of extensions: 9383299 Number of successful extensions: 20805 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 20810 Number of HSP's successfully gapped: 15 Length of query: 169 Length of database: 757,237,578 Length adjustment: 117 Effective length of query: 52 Effective length of database: 439,570,995 Effective search space: 22857691740 Effective search space used: 22857691740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 121 (51.2 bits)