BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0030.p (573 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_358173.1| Histidine kinase [Streptococcus pneumoniae R6] ... 139 2e-31 ref|NP_345167.1| sensor histidine kinase, putative [Streptococcu... 138 3e-31 ref|NP_603097.1| Two-component sensor kinase yesM [Fusobacterium... 122 2e-26 ref|NP_623881.1| predicted ATPase [Thermoanaerobacter tengcongen... 112 2e-23 ref|NP_607635.1| putative histidine kinase [Streptococcus pyogen... 111 5e-23 >ref|NP_358173.1| Histidine kinase [Streptococcus pneumoniae R6] pir||C97944 histidine kinase (EC 2.7.3.-) [imported] - Streptococcus pneumoniae (strain R6) gb|AAK99383.1| Histidine kinase [Streptococcus pneumoniae R6] Length = 563 Score = 139 bits (349), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 109/364 (29%), Positives = 182/364 (50%), Gaps = 34/364 (9%) Query: 192 ISADNLIRPLRQINLGANGYASLVDENGTRISSP----LSGSGKL------VQQEQGW-P 240 IS + L L Q+ LG G+A +++EN + P S S K+ ++ QG+ P Sbjct: 183 ISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQHTVYSSSSKMEAMKPYIETGQGYTP 242 Query: 241 GLLRSRTTISSE-FSNTTFSADMVIQFGAFEKI--MIAQLLIMLLFFIVTSTL-CAVMLF 296 G ++ +S E + T ++ V+ + EK+ + +QLL LL VTS L C +++ Sbjct: 243 G---HKSYVSQEKIAGTDWT---VLGVSSLEKLDQVRSQLLWTLLGASVTSLLVCLCLVW 296 Query: 297 FN-KRVLGPIQNFSENLAHMNEDGQPAGFKSSKIIELEQASDQFKDLMEQVKTFKIAIYE 355 F+ KR + P+++ E + + Q K EL + + QF +++Q+ +AI Sbjct: 297 FSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELREVTRQFNAMLDQIDQLMVAIRS 356 Query: 356 QELEKQRIQLDYMKQQIKPHFFLNCLTSIYSMAQIQMYEEIENMAMSTSKYFRYTFQDGG 415 QE ++ QL + QI PHF N L +I MA+ + + + S + YFR G Sbjct: 357 QEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFHDSQRVVQVTKSLATYFRLALNQGK 416 Query: 416 NFVRLEDEIEHIRIYLEIQRSRYRDAFRHHIVQDETAKDVQIPPLVLQTFIENSVKYAIS 475 + + L DEI H+R YL IQ+ RY D + I ++ ++ +P LVLQ +EN++ + I Sbjct: 417 DLICLSDEINHVRQYLFIQKQRYGDKLEYEINENVAFDNLVLPKLVLQPLVENALYHGIK 476 Query: 476 -RDPEVQISLTVTRRESEEEQVTVIQISDNGPGF---APEVLEKLVRG----QPLDQSKG 527 ++ + I L+V +++S VI+I D+G GF +L RG Q +DQ Sbjct: 477 EKEGQGHIKLSVQKQDSG----LVIRIEDDGVGFQDAGDSSQSQLKRGGVGLQNVDQRLK 532 Query: 528 THIG 531 H G Sbjct: 533 LHFG 536 >ref|NP_345167.1| sensor histidine kinase, putative [Streptococcus pneumoniae TIGR4] pir||F95076 sensor histidine kinase, probable [imported] - Streptococcus pneumoniae (strain TIGR4) emb|CAB54581.1| histidine kinase [Streptococcus pneumoniae] gb|AAF31453.1| putative histidine kinase [Streptococcus pneumoniae] gb|AAK74807.1| sensor histidine kinase, putative [Streptococcus pneumoniae TIGR4] Length = 563 Score = 138 bits (348), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 109/364 (29%), Positives = 181/364 (49%), Gaps = 34/364 (9%) Query: 192 ISADNLIRPLRQINLGANGYASLVDENGTRISSP----LSGSGKL------VQQEQGW-P 240 IS + L L Q+ LG G+A +++EN + P S S K+ + QG+ P Sbjct: 183 ISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQHTVYSSSSKMEAMKPYIDTGQGYTP 242 Query: 241 GLLRSRTTISSE-FSNTTFSADMVIQFGAFEKI--MIAQLLIMLLFFIVTSTL-CAVMLF 296 G ++ +S E + T ++ V+ + EK+ + +QLL LL VTS L C +++ Sbjct: 243 G---HKSYVSQEKIAGTDWT---VLGVSSLEKLDQVRSQLLWTLLGASVTSLLVCLCLVW 296 Query: 297 FN-KRVLGPIQNFSENLAHMNEDGQPAGFKSSKIIELEQASDQFKDLMEQVKTFKIAIYE 355 F+ KR + P+++ E + + Q K EL + + QF +++Q+ +AI Sbjct: 297 FSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELREVTRQFNAMLDQIDQLMVAIRS 356 Query: 356 QELEKQRIQLDYMKQQIKPHFFLNCLTSIYSMAQIQMYEEIENMAMSTSKYFRYTFQDGG 415 QE ++ QL + QI PHF N L +I MA+ + + + S + YFR G Sbjct: 357 QEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFHDSQRVVQVTKSLATYFRLALNQGK 416 Query: 416 NFVRLEDEIEHIRIYLEIQRSRYRDAFRHHIVQDETAKDVQIPPLVLQTFIENSVKYAIS 475 + + L DEI H+R YL IQ+ RY D + I ++ ++ +P LVLQ +EN++ + I Sbjct: 417 DLICLSDEINHVRQYLFIQKQRYGDKLEYEINENVAFDNLVLPKLVLQPLVENALYHGIK 476 Query: 476 -RDPEVQISLTVTRRESEEEQVTVIQISDNGPGF---APEVLEKLVRG----QPLDQSKG 527 ++ + I L+V +++S VI+I D+G GF +L RG Q +DQ Sbjct: 477 EKEGQGHIKLSVQKQDSG----LVIRIEDDGVGFQDAGDSSQSQLKRGGVGLQNVDQRLK 532 Query: 528 THIG 531 H G Sbjct: 533 LHFG 536 >ref|NP_603097.1| Two-component sensor kinase yesM [Fusobacterium nucleatum subsp. nucleatum ATCC 25586] gb|AAL94396.1| Two-component sensor kinase yesM [Fusobacterium nucleatum subsp. nucleatum ATCC 25586] Length = 552 Score = 122 bits (306), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 85/299 (28%), Positives = 151/299 (50%), Gaps = 31/299 (10%) Query: 269 FEKIMIAQLLIMLLFFIVTSTLCAVMLFFNKRVLGPIQNFSENLAHMNEDGQPAGFKSSK 328 FE I+I+ L +L+ ++ ++ +R+ PI+ +++ + N D K Sbjct: 282 FEMIVISCLASLLITVLINISVL-------RRITKPIKELEQHMNNFNNDLSKINLKGDV 334 Query: 329 IIELEQASDQFKDLMEQVKTFKIAIYEQELEKQRIQLDYMKQQIKPHFFLNCLTSIYSMA 388 IE+ + F ++++++K Y +E E ++ + QI PHF N L +I MA Sbjct: 335 SIEILSLQNHFNEMIDKIK------YLREYE-----INALYSQINPHFLYNTLDTIIWMA 383 Query: 389 QIQMYEEIENMAMSTSKYFRYTFQDGGNFVRLEDEIEHIRIYLEIQRSRYRDAFRHHIVQ 448 + Q E++ ++ + S +FR + +G + L++EI HI+ YL IQ+ RY D + I Sbjct: 384 EFQDTEKVISITKALSNFFRISLSNGKEKIPLKEEINHIKEYLYIQKQRYEDKLEYKISI 443 Query: 449 DETAKDVQIPPLVLQTFIENSVKYAISR-DPEVQISLTVTRRESEEEQVTVIQISDNGPG 507 E +++++P ++LQ F+EN++ + I D IS+ E++ E + I DNG G Sbjct: 444 QEELENIEVPKIILQPFVENAIYHGIKNLDTTGIISIYSQIVENKIELI----IEDNGIG 499 Query: 508 FAPEVLEKLVRGQPLDQSKGTHIGIMNTLKRLEYLYYKKAAVHFSNIEGGGASVIITLP 566 F + Q L + G +GI N KR++Y Y K+ + GA +IITLP Sbjct: 500 FEA------AKKQALMKMGG--VGIKNVNKRIQYYYGKEYGAKIDSSFKTGARIIITLP 550 >ref|NP_623881.1| predicted ATPase [Thermoanaerobacter tengcongensis] gb|AAM25485.1| predicted ATPase [Thermoanaerobacter tengcongensis] Length = 593 Score = 112 bits (281), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 76/295 (25%), Positives = 146/295 (49%), Gaps = 12/295 (4%) Query: 278 LIMLLFFIVTSTLCAVMLFFNKRVLGPIQNFSENLAHMNEDG--QPAGFKSSKIIELEQA 335 L +L+F ++ + L V ++F++ + P++ + ++ + + + F+ E+ Sbjct: 303 LRILVFCLLFAFL--VSMYFSRALTHPLERLANSMKKVRQGNLEEKVEFEYHSKDEIAIV 360 Query: 336 SDQFKDLMEQVKTFKIAIYEQELEKQRIQLDYMKQQIKPHFFLNCLTSIYSMAQIQMYEE 395 ++ ++E++ I + E+ K+ +L ++ QI PHF N L +I+ A+ E Sbjct: 361 IKEYNRMVERINDLINRILKLEINKREAELKALEAQINPHFLYNTLDTIFWKAEKAKDRE 420 Query: 396 IENMAMSTSKYFRYTFQDGGNFVRLEDEIEHIRIYLEIQRSRYRDAFRHHIVQDETAKDV 455 + M S S+ FR T G F+++ E E I YL +Q RY++ F++ + + D Sbjct: 421 LSEMIYSLSRLFRLTLNRGEEFIQVRGEKELIERYLFLQSKRYKNKFKYSVKISDEILDY 480 Query: 456 QIPPLVLQTFIENSVKYAISRDPEVQISLTVTRRESEEEQVTVIQISDNGPGFAPEVLEK 515 IP L+LQ F+EN++ + + + E + +E E + I DNGPG + E LEK Sbjct: 481 YIPKLILQPFVENAIVHGLENNSEESFVEVIGEKEGENLKFI---IKDNGPGMSEETLEK 537 Query: 516 LVR---GQPLDQSKGTHIGIMNTLKRLEYLYYKKAAVHFSNIEGGGASVIITLPD 567 L R + +++ G I N +RL Y K + + +G G ++ + LP+ Sbjct: 538 LKRFIESENIEEKVGY--AIRNVNERLRLYYGDKYELKIESRQGEGTTISLILPE 590 >ref|NP_607635.1| putative histidine kinase [Streptococcus pyogenes MGAS8232] gb|AAL98134.1| putative histidine kinase [Streptococcus pyogenes MGAS8232] Length = 577 Score = 111 bits (277), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 74/267 (27%), Positives = 130/267 (48%), Gaps = 12/267 (4%) Query: 278 LIMLLFFIVTSTLCAVMLFFNKRVLGPIQNFSENLAHMNEDGQPAGFKSSKIIELEQASD 337 L M L + L +++ ++ + PIQ + + + + + + + EL + Sbjct: 285 LGMGLLACLICGLTTILVL--RKWIRPIQQLQQVILAIQKGNRQLRAQETGSPELTDLAH 342 Query: 338 QFKDLMEQVKTFKIAIYEQELEKQRIQLDYMKQQIKPHFFLNCLTSIYSMAQIQMYEEIE 397 QF L++Q+ + +A+ ++E E + +L + QI PHF N L +I MA+ + + Sbjct: 343 QFNALLDQIDSLMVAVADKEKEIGQYRLQALASQINPHFLYNTLDTIIWMAEFNDSKRVV 402 Query: 398 NMAMSTSKYFRYTFQDGGNFVRLEDEIEHIRIYLEIQRSRYRDAFRHHIVQDETAKDVQI 457 + S +KYFR G +++RL DE++H+ YL IQ+ RY D + + + D I Sbjct: 403 EVTKSLAKYFRLALNQGNDYIRLADELDHVSQYLFIQKQRYGDKLTYDVQGLDAYADFII 462 Query: 458 PPLVLQTFIENSVKYAISR-DPEVQISLTVTRRESEEEQVTVIQISDNGPGFAPEVLEKL 516 P L+LQ +EN++ + I D + I +TV SE Q ++ + DNG G L Sbjct: 463 PKLILQPLVENAIYHGIKEVDRKGMIKVTV----SETAQHLILTVWDNGKGIEASA---L 515 Query: 517 VRGQPLDQSKGTHIGIMNTLKRLEYLY 543 Q L G +G+ N +RL+ Y Sbjct: 516 TNSQSLLTRGG--VGLKNVDQRLKLQY 540 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 815,535,384 Number of extensions: 34673737 Number of successful extensions: 108577 Number of sequences better than 1.0e-05: 158 Number of HSP's gapped: 108438 Number of HSP's successfully gapped: 158 Length of query: 573 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 442 Effective length of database: 401,559,609 Effective search space: 177489347178 Effective search space used: 177489347178 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)